id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342785-55r01n0x	Lemmon, Gordon H	Predicting the sensitivity and specificity of published real-time PCR assays	2008-09-25		.txt	text/plain	4317	239	52	METHODS: We assessed the quality of a signature by predicting the number of true positive, false positive and false negative hits against all available public sequence data. This analysis must include the predicted false negative and false positive rates for the developed signatures, and consider all available public sequence data. A freely available real time PCR analysis tool called TaqSim [4] was used to find public sequences that would match the primer/probe assay in question. However, according to the genomic data available, a better match of primers and probes to target is possible and is usually desired for high sensitivity detection. Current real-time PCR assay design approaches produce signatures with sensitivities generally too low for clinical use. Fifty Seven TaqMan PCR primer/probe combinations we predict to have higher sensitivity/specificity than current published assays. Development of quantitative gene-specific real-time RT-PCR assays for the detection of measles virus in clinical specimens	./cache/cord-342785-55r01n0x.txt	./txt/cord-342785-55r01n0x.txt