id	sid	tid	token	lemma	pos
cord-342785-55r01n0x	1	1	key	key	JJ
cord-342785-55r01n0x	1	2	:	:	:
cord-342785-55r01n0x	1	3	cord-342785	cord-342785	NNP
cord-342785-55r01n0x	1	4	-	-	HYPH
cord-342785-55r01n0x	1	5	55r01n0x	55r01n0x	CD
cord-342785-55r01n0x	1	6	authors	author	NNS
cord-342785-55r01n0x	1	7	:	:	:
cord-342785-55r01n0x	1	8	Lemmon	Lemmon	NNP
cord-342785-55r01n0x	1	9	,	,	,
cord-342785-55r01n0x	1	10	Gordon	Gordon	NNP
cord-342785-55r01n0x	1	11	H	H	NNP
cord-342785-55r01n0x	1	12	;	;	:
cord-342785-55r01n0x	1	13	Gardner	Gardner	NNP
cord-342785-55r01n0x	1	14	,	,	,
cord-342785-55r01n0x	1	15	Shea	Shea	NNP
cord-342785-55r01n0x	1	16	N	N	NNP
cord-342785-55r01n0x	1	17	title	title	NN
cord-342785-55r01n0x	1	18	:	:	:
cord-342785-55r01n0x	1	19	Predicting	predict	VBG
cord-342785-55r01n0x	1	20	the	the	DT
cord-342785-55r01n0x	1	21	sensitivity	sensitivity	NN
cord-342785-55r01n0x	1	22	and	and	CC
cord-342785-55r01n0x	1	23	specificity	specificity	NN
cord-342785-55r01n0x	1	24	of	of	IN
cord-342785-55r01n0x	1	25	published	publish	VBN
cord-342785-55r01n0x	1	26	real	real	JJ
cord-342785-55r01n0x	1	27	-	-	HYPH
cord-342785-55r01n0x	1	28	time	time	NN
cord-342785-55r01n0x	1	29	PCR	PCR	NNP
cord-342785-55r01n0x	1	30	assays	assay	NNS
cord-342785-55r01n0x	1	31	date	date	NN
cord-342785-55r01n0x	1	32	:	:	:
cord-342785-55r01n0x	1	33	2008	2008	CD
cord-342785-55r01n0x	1	34	-	-	SYM
cord-342785-55r01n0x	1	35	09	09	CD
cord-342785-55r01n0x	1	36	-	-	SYM
cord-342785-55r01n0x	1	37	25	25	CD
cord-342785-55r01n0x	1	38	journal	journal	NN
cord-342785-55r01n0x	1	39	:	:	:
cord-342785-55r01n0x	2	1	Ann	Ann	NNP
cord-342785-55r01n0x	2	2	Clin	Clin	NNP
cord-342785-55r01n0x	2	3	Microbiol	Microbiol	NNP
cord-342785-55r01n0x	2	4	Antimicrob	Antimicrob	NNP
cord-342785-55r01n0x	2	5	DOI	DOI	NNP
cord-342785-55r01n0x	2	6	:	:	:
cord-342785-55r01n0x	2	7	10.1186/1476	10.1186/1476	CD
cord-342785-55r01n0x	2	8	-	-	HYPH
cord-342785-55r01n0x	2	9	0711	0711	CD
cord-342785-55r01n0x	2	10	-	-	HYPH
cord-342785-55r01n0x	2	11	7	7	CD
cord-342785-55r01n0x	2	12	-	-	SYM
cord-342785-55r01n0x	2	13	18	18	CD
cord-342785-55r01n0x	2	14	sha	sha	NNP
cord-342785-55r01n0x	2	15	:	:	:
cord-342785-55r01n0x	3	1	2a98b0a9e3e89d1aff14a6f64e25374b4e2855a8	2a98b0a9e3e89d1aff14a6f64e25374b4e2855a8	CD
cord-342785-55r01n0x	3	2	doc_id	doc_id	CD
cord-342785-55r01n0x	3	3	:	:	:
cord-342785-55r01n0x	4	1	342785	342785	CD
cord-342785-55r01n0x	4	2	cord_uid	cord_uid	NN
cord-342785-55r01n0x	4	3	:	:	:
cord-342785-55r01n0x	5	1	55r01n0x	55r01n0x	CD
cord-342785-55r01n0x	5	2	BACKGROUND	background	NN
cord-342785-55r01n0x	5	3	:	:	:
cord-342785-55r01n0x	6	1	In	in	IN
cord-342785-55r01n0x	6	2	recent	recent	JJ
cord-342785-55r01n0x	6	3	years	year	NNS
cord-342785-55r01n0x	6	4	real	real	JJ
cord-342785-55r01n0x	6	5	-	-	HYPH
cord-342785-55r01n0x	6	6	time	time	NN
cord-342785-55r01n0x	6	7	PCR	PCR	NNP
cord-342785-55r01n0x	6	8	has	have	VBZ
cord-342785-55r01n0x	6	9	become	become	VBN
cord-342785-55r01n0x	6	10	a	a	DT
cord-342785-55r01n0x	6	11	leading	leading	JJ
cord-342785-55r01n0x	6	12	technique	technique	NN
cord-342785-55r01n0x	6	13	for	for	IN
cord-342785-55r01n0x	6	14	nucleic	nucleic	JJ
cord-342785-55r01n0x	6	15	acid	acid	NN
cord-342785-55r01n0x	6	16	detection	detection	NN
cord-342785-55r01n0x	6	17	and	and	CC
cord-342785-55r01n0x	6	18	quantification	quantification	NN
cord-342785-55r01n0x	6	19	.	.	.
cord-342785-55r01n0x	7	1	These	these	DT
cord-342785-55r01n0x	7	2	assays	assay	NNS
cord-342785-55r01n0x	7	3	have	have	VBP
cord-342785-55r01n0x	7	4	the	the	DT
cord-342785-55r01n0x	7	5	potential	potential	NN
cord-342785-55r01n0x	7	6	to	to	TO
cord-342785-55r01n0x	7	7	greatly	greatly	RB
cord-342785-55r01n0x	7	8	enhance	enhance	VB
cord-342785-55r01n0x	7	9	efficiency	efficiency	NN
cord-342785-55r01n0x	7	10	in	in	IN
cord-342785-55r01n0x	7	11	the	the	DT
cord-342785-55r01n0x	7	12	clinical	clinical	JJ
cord-342785-55r01n0x	7	13	laboratory	laboratory	NN
cord-342785-55r01n0x	7	14	.	.	.
cord-342785-55r01n0x	8	1	Choice	choice	NN
cord-342785-55r01n0x	8	2	of	of	IN
cord-342785-55r01n0x	8	3	primer	primer	NN
cord-342785-55r01n0x	8	4	and	and	CC
cord-342785-55r01n0x	8	5	probe	probe	NN
cord-342785-55r01n0x	8	6	sequences	sequence	NNS
cord-342785-55r01n0x	8	7	is	be	VBZ
cord-342785-55r01n0x	8	8	critical	critical	JJ
cord-342785-55r01n0x	8	9	for	for	IN
cord-342785-55r01n0x	8	10	accurate	accurate	JJ
cord-342785-55r01n0x	8	11	diagnosis	diagnosis	NN
cord-342785-55r01n0x	8	12	in	in	IN
cord-342785-55r01n0x	8	13	the	the	DT
cord-342785-55r01n0x	8	14	clinic	clinic	NN
cord-342785-55r01n0x	8	15	,	,	,
cord-342785-55r01n0x	8	16	yet	yet	CC
cord-342785-55r01n0x	8	17	current	current	JJ
cord-342785-55r01n0x	8	18	primer	primer	NN
cord-342785-55r01n0x	8	19	/	/	SYM
cord-342785-55r01n0x	8	20	probe	probe	NN
cord-342785-55r01n0x	8	21	signature	signature	NN
cord-342785-55r01n0x	8	22	design	design	NN
cord-342785-55r01n0x	8	23	strategies	strategy	NNS
cord-342785-55r01n0x	8	24	are	be	VBP
cord-342785-55r01n0x	8	25	limited	limited	JJ
cord-342785-55r01n0x	8	26	,	,	,
cord-342785-55r01n0x	8	27	and	and	CC
cord-342785-55r01n0x	8	28	signature	signature	NN
cord-342785-55r01n0x	8	29	evaluation	evaluation	NN
cord-342785-55r01n0x	8	30	methods	method	NNS
cord-342785-55r01n0x	8	31	are	be	VBP
cord-342785-55r01n0x	8	32	lacking	lack	VBG
cord-342785-55r01n0x	8	33	.	.	.
cord-342785-55r01n0x	9	1	METHODS	methods	NN
cord-342785-55r01n0x	9	2	:	:	:
cord-342785-55r01n0x	10	1	We	-PRON-	PRP
cord-342785-55r01n0x	10	2	assessed	assess	VBD
cord-342785-55r01n0x	10	3	the	the	DT
cord-342785-55r01n0x	10	4	quality	quality	NN
cord-342785-55r01n0x	10	5	of	of	IN
cord-342785-55r01n0x	10	6	a	a	DT
cord-342785-55r01n0x	10	7	signature	signature	NN
cord-342785-55r01n0x	10	8	by	by	IN
cord-342785-55r01n0x	10	9	predicting	predict	VBG
cord-342785-55r01n0x	10	10	the	the	DT
cord-342785-55r01n0x	10	11	number	number	NN
cord-342785-55r01n0x	10	12	of	of	IN
cord-342785-55r01n0x	10	13	true	true	JJ
cord-342785-55r01n0x	10	14	positive	positive	JJ
cord-342785-55r01n0x	10	15	,	,	,
cord-342785-55r01n0x	10	16	false	false	JJ
cord-342785-55r01n0x	10	17	positive	positive	JJ
cord-342785-55r01n0x	10	18	and	and	CC
cord-342785-55r01n0x	10	19	false	false	JJ
cord-342785-55r01n0x	10	20	negative	negative	JJ
cord-342785-55r01n0x	10	21	hits	hit	NNS
cord-342785-55r01n0x	10	22	against	against	IN
cord-342785-55r01n0x	10	23	all	all	DT
cord-342785-55r01n0x	10	24	available	available	JJ
cord-342785-55r01n0x	10	25	public	public	JJ
cord-342785-55r01n0x	10	26	sequence	sequence	NN
cord-342785-55r01n0x	10	27	data	datum	NNS
cord-342785-55r01n0x	10	28	.	.	.
cord-342785-55r01n0x	11	1	We	-PRON-	PRP
cord-342785-55r01n0x	11	2	found	find	VBD
cord-342785-55r01n0x	11	3	real	real	JJ
cord-342785-55r01n0x	11	4	-	-	HYPH
cord-342785-55r01n0x	11	5	time	time	NN
cord-342785-55r01n0x	11	6	PCR	PCR	NNP
cord-342785-55r01n0x	11	7	signatures	signature	NNS
cord-342785-55r01n0x	11	8	described	describe	VBN
cord-342785-55r01n0x	11	9	in	in	IN
cord-342785-55r01n0x	11	10	recent	recent	JJ
cord-342785-55r01n0x	11	11	literature	literature	NN
cord-342785-55r01n0x	11	12	and	and	CC
cord-342785-55r01n0x	11	13	used	use	VBD
cord-342785-55r01n0x	11	14	a	a	DT
cord-342785-55r01n0x	11	15	BLAST	blast	NN
cord-342785-55r01n0x	11	16	search	search	NN
cord-342785-55r01n0x	11	17	based	base	VBN
cord-342785-55r01n0x	11	18	approach	approach	NN
cord-342785-55r01n0x	11	19	to	to	TO
cord-342785-55r01n0x	11	20	collect	collect	VB
cord-342785-55r01n0x	11	21	all	all	DT
cord-342785-55r01n0x	11	22	hits	hit	NNS
cord-342785-55r01n0x	11	23	to	to	IN
cord-342785-55r01n0x	11	24	the	the	DT
cord-342785-55r01n0x	11	25	primer	primer	NN
cord-342785-55r01n0x	11	26	-	-	HYPH
cord-342785-55r01n0x	11	27	probe	probe	NN
cord-342785-55r01n0x	11	28	combinations	combination	NNS
cord-342785-55r01n0x	11	29	that	that	WDT
cord-342785-55r01n0x	11	30	should	should	MD
cord-342785-55r01n0x	11	31	be	be	VB
cord-342785-55r01n0x	11	32	amplified	amplify	VBN
cord-342785-55r01n0x	11	33	by	by	IN
cord-342785-55r01n0x	11	34	real	real	JJ
cord-342785-55r01n0x	11	35	-	-	HYPH
cord-342785-55r01n0x	11	36	time	time	NN
cord-342785-55r01n0x	11	37	PCR	PCR	NNP
cord-342785-55r01n0x	11	38	chemistry	chemistry	NN
cord-342785-55r01n0x	11	39	.	.	.
cord-342785-55r01n0x	12	1	We	-PRON-	PRP
cord-342785-55r01n0x	12	2	then	then	RB
cord-342785-55r01n0x	12	3	compared	compare	VBD
cord-342785-55r01n0x	12	4	our	-PRON-	PRP$
cord-342785-55r01n0x	12	5	hits	hit	NNS
cord-342785-55r01n0x	12	6	with	with	IN
cord-342785-55r01n0x	12	7	the	the	DT
cord-342785-55r01n0x	12	8	sequences	sequence	NNS
cord-342785-55r01n0x	12	9	in	in	IN
cord-342785-55r01n0x	12	10	the	the	DT
cord-342785-55r01n0x	12	11	NCBI	NCBI	NNP
cord-342785-55r01n0x	12	12	taxonomy	taxonomy	NN
cord-342785-55r01n0x	12	13	tree	tree	NN
cord-342785-55r01n0x	12	14	that	that	WDT
cord-342785-55r01n0x	12	15	the	the	DT
cord-342785-55r01n0x	12	16	signature	signature	NN
cord-342785-55r01n0x	12	17	was	be	VBD
cord-342785-55r01n0x	12	18	designed	design	VBN
cord-342785-55r01n0x	12	19	to	to	TO
cord-342785-55r01n0x	12	20	detect	detect	VB
cord-342785-55r01n0x	12	21	.	.	.
cord-342785-55r01n0x	13	1	RESULTS	result	NNS
cord-342785-55r01n0x	13	2	:	:	:
cord-342785-55r01n0x	14	1	We	-PRON-	PRP
cord-342785-55r01n0x	14	2	found	find	VBD
cord-342785-55r01n0x	14	3	that	that	IN
cord-342785-55r01n0x	14	4	many	many	JJ
cord-342785-55r01n0x	14	5	published	publish	VBN
cord-342785-55r01n0x	14	6	signatures	signature	NNS
cord-342785-55r01n0x	14	7	have	have	VBP
cord-342785-55r01n0x	14	8	high	high	JJ
cord-342785-55r01n0x	14	9	specificity	specificity	NN
cord-342785-55r01n0x	14	10	(	(	-LRB-
cord-342785-55r01n0x	14	11	almost	almost	RB
cord-342785-55r01n0x	14	12	no	no	DT
cord-342785-55r01n0x	14	13	false	false	JJ
cord-342785-55r01n0x	14	14	positives	positive	NNS
cord-342785-55r01n0x	14	15	)	)	-RRB-
cord-342785-55r01n0x	14	16	but	but	CC
cord-342785-55r01n0x	14	17	low	low	JJ
cord-342785-55r01n0x	14	18	sensitivity	sensitivity	NN
cord-342785-55r01n0x	14	19	(	(	-LRB-
cord-342785-55r01n0x	14	20	high	high	JJ
cord-342785-55r01n0x	14	21	false	false	JJ
cord-342785-55r01n0x	14	22	negative	negative	JJ
cord-342785-55r01n0x	14	23	rate	rate	NN
cord-342785-55r01n0x	14	24	)	)	-RRB-
cord-342785-55r01n0x	14	25	.	.	.
cord-342785-55r01n0x	15	1	Where	where	WRB
cord-342785-55r01n0x	15	2	high	high	JJ
cord-342785-55r01n0x	15	3	sensitivity	sensitivity	NN
cord-342785-55r01n0x	15	4	is	be	VBZ
cord-342785-55r01n0x	15	5	needed	need	VBN
cord-342785-55r01n0x	15	6	,	,	,
cord-342785-55r01n0x	15	7	we	-PRON-	PRP
cord-342785-55r01n0x	15	8	offer	offer	VBP
cord-342785-55r01n0x	15	9	a	a	DT
cord-342785-55r01n0x	15	10	revised	revise	VBN
cord-342785-55r01n0x	15	11	methodology	methodology	NN
cord-342785-55r01n0x	15	12	for	for	IN
cord-342785-55r01n0x	15	13	signature	signature	NN
cord-342785-55r01n0x	15	14	design	design	NN
cord-342785-55r01n0x	15	15	which	which	WDT
cord-342785-55r01n0x	15	16	may	may	MD
cord-342785-55r01n0x	15	17	designate	designate	VB
cord-342785-55r01n0x	15	18	that	that	IN
cord-342785-55r01n0x	15	19	multiple	multiple	JJ
cord-342785-55r01n0x	15	20	signatures	signature	NNS
cord-342785-55r01n0x	15	21	are	be	VBP
cord-342785-55r01n0x	15	22	required	require	VBN
cord-342785-55r01n0x	15	23	to	to	TO
cord-342785-55r01n0x	15	24	detect	detect	VB
cord-342785-55r01n0x	15	25	all	all	DT
cord-342785-55r01n0x	15	26	sequenced	sequence	VBN
cord-342785-55r01n0x	15	27	strains	strain	NNS
cord-342785-55r01n0x	15	28	.	.	.
cord-342785-55r01n0x	16	1	We	-PRON-	PRP
cord-342785-55r01n0x	16	2	use	use	VBP
cord-342785-55r01n0x	16	3	this	this	DT
cord-342785-55r01n0x	16	4	methodology	methodology	NN
cord-342785-55r01n0x	16	5	to	to	TO
cord-342785-55r01n0x	16	6	produce	produce	VB
cord-342785-55r01n0x	16	7	new	new	JJ
cord-342785-55r01n0x	16	8	signatures	signature	NNS
cord-342785-55r01n0x	16	9	that	that	WDT
cord-342785-55r01n0x	16	10	are	be	VBP
cord-342785-55r01n0x	16	11	predicted	predict	VBN
cord-342785-55r01n0x	16	12	to	to	TO
cord-342785-55r01n0x	16	13	have	have	VB
cord-342785-55r01n0x	16	14	higher	high	JJR
cord-342785-55r01n0x	16	15	sensitivity	sensitivity	NN
cord-342785-55r01n0x	16	16	and	and	CC
cord-342785-55r01n0x	16	17	specificity	specificity	NN
cord-342785-55r01n0x	16	18	.	.	.
cord-342785-55r01n0x	17	1	CONCLUSION	conclusion	NN
cord-342785-55r01n0x	17	2	:	:	:
cord-342785-55r01n0x	18	1	We	-PRON-	PRP
cord-342785-55r01n0x	18	2	show	show	VBP
cord-342785-55r01n0x	18	3	that	that	IN
cord-342785-55r01n0x	18	4	current	current	JJ
cord-342785-55r01n0x	18	5	methods	method	NNS
cord-342785-55r01n0x	18	6	for	for	IN
cord-342785-55r01n0x	18	7	real	real	JJ
cord-342785-55r01n0x	18	8	-	-	HYPH
cord-342785-55r01n0x	18	9	time	time	NN
cord-342785-55r01n0x	18	10	PCR	PCR	NNP
cord-342785-55r01n0x	18	11	assay	assay	NN
cord-342785-55r01n0x	18	12	design	design	NN
cord-342785-55r01n0x	18	13	have	have	VBP
cord-342785-55r01n0x	18	14	unacceptably	unacceptably	RB
cord-342785-55r01n0x	18	15	low	low	JJ
cord-342785-55r01n0x	18	16	sensitivities	sensitivity	NNS
cord-342785-55r01n0x	18	17	for	for	IN
cord-342785-55r01n0x	18	18	most	most	JJS
cord-342785-55r01n0x	18	19	clinical	clinical	JJ
cord-342785-55r01n0x	18	20	applications	application	NNS
cord-342785-55r01n0x	18	21	.	.	.
cord-342785-55r01n0x	19	1	Additionally	additionally	RB
cord-342785-55r01n0x	19	2	,	,	,
cord-342785-55r01n0x	19	3	as	as	IN
cord-342785-55r01n0x	19	4	new	new	JJ
cord-342785-55r01n0x	19	5	sequence	sequence	NN
cord-342785-55r01n0x	19	6	data	data	NN
cord-342785-55r01n0x	19	7	becomes	become	VBZ
cord-342785-55r01n0x	19	8	available	available	JJ
cord-342785-55r01n0x	19	9	,	,	,
cord-342785-55r01n0x	19	10	old	old	JJ
cord-342785-55r01n0x	19	11	assays	assay	NNS
cord-342785-55r01n0x	19	12	must	must	MD
cord-342785-55r01n0x	19	13	be	be	VB
cord-342785-55r01n0x	19	14	reassessed	reassess	VBN
cord-342785-55r01n0x	19	15	and	and	CC
cord-342785-55r01n0x	19	16	redesigned	redesign	VBN
cord-342785-55r01n0x	19	17	.	.	.
cord-342785-55r01n0x	20	1	A	a	DT
cord-342785-55r01n0x	20	2	standard	standard	JJ
cord-342785-55r01n0x	20	3	protocol	protocol	NN
cord-342785-55r01n0x	20	4	for	for	IN
cord-342785-55r01n0x	20	5	both	both	CC
cord-342785-55r01n0x	20	6	generating	generate	VBG
cord-342785-55r01n0x	20	7	and	and	CC
cord-342785-55r01n0x	20	8	assessing	assess	VBG
cord-342785-55r01n0x	20	9	the	the	DT
cord-342785-55r01n0x	20	10	quality	quality	NN
cord-342785-55r01n0x	20	11	of	of	IN
cord-342785-55r01n0x	20	12	these	these	DT
cord-342785-55r01n0x	20	13	assays	assay	NNS
cord-342785-55r01n0x	20	14	is	be	VBZ
cord-342785-55r01n0x	20	15	therefore	therefore	RB
cord-342785-55r01n0x	20	16	of	of	IN
cord-342785-55r01n0x	20	17	great	great	JJ
cord-342785-55r01n0x	20	18	value	value	NN
cord-342785-55r01n0x	20	19	.	.	.
cord-342785-55r01n0x	21	1	Real	real	JJ
cord-342785-55r01n0x	21	2	-	-	HYPH
cord-342785-55r01n0x	21	3	time	time	NN
cord-342785-55r01n0x	21	4	PCR	PCR	NNP
cord-342785-55r01n0x	21	5	has	have	VBZ
cord-342785-55r01n0x	21	6	the	the	DT
cord-342785-55r01n0x	21	7	capacity	capacity	NN
cord-342785-55r01n0x	21	8	to	to	TO
cord-342785-55r01n0x	21	9	greatly	greatly	RB
cord-342785-55r01n0x	21	10	improve	improve	VB
cord-342785-55r01n0x	21	11	clinical	clinical	JJ
cord-342785-55r01n0x	21	12	diagnostics	diagnostic	NNS
cord-342785-55r01n0x	21	13	.	.	.
cord-342785-55r01n0x	22	1	The	the	DT
cord-342785-55r01n0x	22	2	improved	improved	JJ
cord-342785-55r01n0x	22	3	assay	assay	NN
cord-342785-55r01n0x	22	4	design	design	NN
cord-342785-55r01n0x	22	5	and	and	CC
cord-342785-55r01n0x	22	6	evaluation	evaluation	NN
cord-342785-55r01n0x	22	7	methods	method	NNS
cord-342785-55r01n0x	22	8	presented	present	VBN
cord-342785-55r01n0x	22	9	herein	herein	RB
cord-342785-55r01n0x	22	10	will	will	MD
cord-342785-55r01n0x	22	11	expedite	expedite	VB
cord-342785-55r01n0x	22	12	adoption	adoption	NN
cord-342785-55r01n0x	22	13	of	of	IN
cord-342785-55r01n0x	22	14	this	this	DT
cord-342785-55r01n0x	22	15	technique	technique	NN
cord-342785-55r01n0x	22	16	in	in	IN
cord-342785-55r01n0x	22	17	the	the	DT
cord-342785-55r01n0x	22	18	clinical	clinical	JJ
cord-342785-55r01n0x	22	19	lab	lab	NN
cord-342785-55r01n0x	22	20	.	.	.
cord-342785-55r01n0x	23	1	Real	real	JJ
cord-342785-55r01n0x	23	2	-	-	HYPH
cord-342785-55r01n0x	23	3	time	time	NN
cord-342785-55r01n0x	23	4	PCR	PCR	NNP
cord-342785-55r01n0x	23	5	assays	assay	NNS
cord-342785-55r01n0x	23	6	are	be	VBP
cord-342785-55r01n0x	23	7	gaining	gain	VBG
cord-342785-55r01n0x	23	8	popularity	popularity	NN
cord-342785-55r01n0x	23	9	as	as	IN
cord-342785-55r01n0x	23	10	a	a	DT
cord-342785-55r01n0x	23	11	clinical	clinical	JJ
cord-342785-55r01n0x	23	12	tool	tool	NN
cord-342785-55r01n0x	23	13	for	for	IN
cord-342785-55r01n0x	23	14	detecting	detect	VBG
cord-342785-55r01n0x	23	15	and	and	CC
cord-342785-55r01n0x	23	16	quantifying	quantify	VBG
cord-342785-55r01n0x	23	17	the	the	DT
cord-342785-55r01n0x	23	18	presence	presence	NN
cord-342785-55r01n0x	23	19	of	of	IN
cord-342785-55r01n0x	23	20	both	both	DT
cord-342785-55r01n0x	23	21	viral	viral	JJ
cord-342785-55r01n0x	23	22	and	and	CC
cord-342785-55r01n0x	23	23	bacterial	bacterial	JJ
cord-342785-55r01n0x	23	24	pathogens	pathogen	NNS
cord-342785-55r01n0x	23	25	,	,	,
cord-342785-55r01n0x	23	26	as	as	IN
cord-342785-55r01n0x	23	27	reviewed	review	VBN
cord-342785-55r01n0x	23	28	in	in	IN
cord-342785-55r01n0x	23	29	[	[	-LRB-
cord-342785-55r01n0x	23	30	1	1	CD
cord-342785-55r01n0x	23	31	]	]	-RRB-
cord-342785-55r01n0x	23	32	.	.	.
cord-342785-55r01n0x	24	1	Com	com	NN
cord-342785-55r01n0x	24	2	-	-	HYPH
cord-342785-55r01n0x	24	3	pared	pare	VBN
cord-342785-55r01n0x	24	4	to	to	IN
cord-342785-55r01n0x	24	5	traditional	traditional	JJ
cord-342785-55r01n0x	24	6	culturing	culturing	NN
cord-342785-55r01n0x	24	7	methods	method	NNS
cord-342785-55r01n0x	24	8	used	use	VBN
cord-342785-55r01n0x	24	9	in	in	IN
cord-342785-55r01n0x	24	10	identification	identification	NN
cord-342785-55r01n0x	24	11	,	,	,
cord-342785-55r01n0x	24	12	real	real	JJ
cord-342785-55r01n0x	24	13	-	-	HYPH
cord-342785-55r01n0x	24	14	time	time	NN
cord-342785-55r01n0x	24	15	PCR	PCR	NNP
cord-342785-55r01n0x	24	16	is	be	VBZ
cord-342785-55r01n0x	24	17	fast	fast	JJ
cord-342785-55r01n0x	24	18	and	and	CC
cord-342785-55r01n0x	24	19	cost	cost	VB
cord-342785-55r01n0x	24	20	effective	effective	JJ
cord-342785-55r01n0x	24	21	.	.	.
cord-342785-55r01n0x	25	1	In	in	IN
cord-342785-55r01n0x	25	2	addition	addition	NN
cord-342785-55r01n0x	25	3	,	,	,
cord-342785-55r01n0x	25	4	it	-PRON-	PRP
cord-342785-55r01n0x	25	5	can	can	MD
cord-342785-55r01n0x	25	6	be	be	VB
cord-342785-55r01n0x	25	7	quantitative	quantitative	JJ
cord-342785-55r01n0x	25	8	and	and	CC
cord-342785-55r01n0x	25	9	sensitive	sensitive	JJ
cord-342785-55r01n0x	25	10	,	,	,
cord-342785-55r01n0x	25	11	in	in	IN
cord-342785-55r01n0x	25	12	some	some	DT
cord-342785-55r01n0x	25	13	cases	case	NNS
cord-342785-55r01n0x	25	14	greatly	greatly	RB
cord-342785-55r01n0x	25	15	exceeding	exceed	VBG
cord-342785-55r01n0x	25	16	the	the	DT
cord-342785-55r01n0x	25	17	sensitivity	sensitivity	NN
cord-342785-55r01n0x	25	18	for	for	IN
cord-342785-55r01n0x	25	19	conventional	conventional	JJ
cord-342785-55r01n0x	25	20	testing	testing	NN
cord-342785-55r01n0x	25	21	meth	meth	NNS
cord-342785-55r01n0x	25	22	-	-	HYPH
cord-342785-55r01n0x	25	23	ods	od	NNS
cord-342785-55r01n0x	25	24	.	.	.
cord-342785-55r01n0x	26	1	Commercially	commercially	RB
cord-342785-55r01n0x	26	2	distributed	distribute	VBN
cord-342785-55r01n0x	26	3	kits	kit	NNS
cord-342785-55r01n0x	26	4	are	be	VBP
cord-342785-55r01n0x	26	5	available	available	JJ
cord-342785-55r01n0x	26	6	for	for	IN
cord-342785-55r01n0x	26	7	PCRbased	pcrbased	JJ
cord-342785-55r01n0x	26	8	pathogen	pathogen	NN
cord-342785-55r01n0x	26	9	diagnostics	diagnostic	NNS
cord-342785-55r01n0x	26	10	,	,	,
cord-342785-55r01n0x	26	11	and	and	CC
cord-342785-55r01n0x	26	12	PCR	PCR	NNP
cord-342785-55r01n0x	26	13	is	be	VBZ
cord-342785-55r01n0x	26	14	no	no	RB
cord-342785-55r01n0x	26	15	longer	long	RBR
cord-342785-55r01n0x	26	16	thought	think	VBN
cord-342785-55r01n0x	26	17	of	of	IN
cord-342785-55r01n0x	26	18	merely	merely	RB
cord-342785-55r01n0x	26	19	as	as	IN
cord-342785-55r01n0x	26	20	confirmatory	confirmatory	JJ
cord-342785-55r01n0x	26	21	to	to	IN
cord-342785-55r01n0x	26	22	culture	culture	NN
cord-342785-55r01n0x	26	23	.	.	.
cord-342785-55r01n0x	27	1	However	however	RB
cord-342785-55r01n0x	27	2	real	real	JJ
cord-342785-55r01n0x	27	3	-	-	HYPH
cord-342785-55r01n0x	27	4	time	time	NN
cord-342785-55r01n0x	27	5	PCR	PCR	NNP
cord-342785-55r01n0x	27	6	assays	assay	NNS
cord-342785-55r01n0x	27	7	are	be	VBP
cord-342785-55r01n0x	27	8	limited	limit	VBN
cord-342785-55r01n0x	27	9	by	by	IN
cord-342785-55r01n0x	27	10	the	the	DT
cord-342785-55r01n0x	27	11	quality	quality	NN
cord-342785-55r01n0x	27	12	of	of	IN
cord-342785-55r01n0x	27	13	the	the	DT
cord-342785-55r01n0x	27	14	primers	primer	NNS
cord-342785-55r01n0x	27	15	and	and	CC
cord-342785-55r01n0x	27	16	probes	probe	NNS
cord-342785-55r01n0x	27	17	chosen	choose	VBN
cord-342785-55r01n0x	27	18	.	.	.
cord-342785-55r01n0x	28	1	These	these	DT
cord-342785-55r01n0x	28	2	primers	primer	NNS
cord-342785-55r01n0x	28	3	and	and	CC
cord-342785-55r01n0x	28	4	probes	probe	NNS
cord-342785-55r01n0x	28	5	must	must	MD
cord-342785-55r01n0x	28	6	be	be	VB
cord-342785-55r01n0x	28	7	sensitive	sensitive	JJ
cord-342785-55r01n0x	28	8	enough	enough	RB
cord-342785-55r01n0x	28	9	to	to	TO
cord-342785-55r01n0x	28	10	match	match	VB
cord-342785-55r01n0x	28	11	all	all	DT
cord-342785-55r01n0x	28	12	target	target	NN
cord-342785-55r01n0x	28	13	organisms	organism	NNS
cord-342785-55r01n0x	28	14	yet	yet	CC
cord-342785-55r01n0x	28	15	specific	specific	JJ
cord-342785-55r01n0x	28	16	enough	enough	RB
cord-342785-55r01n0x	28	17	to	to	TO
cord-342785-55r01n0x	28	18	exclude	exclude	VB
cord-342785-55r01n0x	28	19	all	all	DT
cord-342785-55r01n0x	28	20	others	other	NNS
cord-342785-55r01n0x	28	21	.	.	.
cord-342785-55r01n0x	29	1	A	a	DT
cord-342785-55r01n0x	29	2	common	common	JJ
cord-342785-55r01n0x	29	3	approach	approach	NN
cord-342785-55r01n0x	29	4	to	to	IN
cord-342785-55r01n0x	29	5	developing	develop	VBG
cord-342785-55r01n0x	29	6	a	a	DT
cord-342785-55r01n0x	29	7	primer	primer	NN
cord-342785-55r01n0x	29	8	/	/	SYM
cord-342785-55r01n0x	29	9	probe	probe	NN
cord-342785-55r01n0x	29	10	combination	combination	NN
cord-342785-55r01n0x	29	11	is	be	VBZ
cord-342785-55r01n0x	29	12	by	by	IN
cord-342785-55r01n0x	29	13	using	use	VBG
cord-342785-55r01n0x	29	14	commercial	commercial	JJ
cord-342785-55r01n0x	29	15	software	software	NN
cord-342785-55r01n0x	29	16	such	such	JJ
cord-342785-55r01n0x	29	17	as	as	IN
cord-342785-55r01n0x	29	18	PrimerExpress	PrimerExpress	NNP
cord-342785-55r01n0x	29	19	®	®	.
cord-342785-55r01n0x	29	20	(	(	-LRB-
cord-342785-55r01n0x	29	21	Applied	Applied	NNP
cord-342785-55r01n0x	29	22	Biosystems	Biosystems	NNP
cord-342785-55r01n0x	29	23	,	,	,
cord-342785-55r01n0x	29	24	Foster	Foster	NNP
cord-342785-55r01n0x	29	25	City	City	NNP
cord-342785-55r01n0x	29	26	,	,	,
cord-342785-55r01n0x	29	27	CA	CA	NNP
cord-342785-55r01n0x	29	28	,	,	,
cord-342785-55r01n0x	29	29	USA	USA	NNP
cord-342785-55r01n0x	29	30	)	)	-RRB-
cord-342785-55r01n0x	29	31	.	.	.
cord-342785-55r01n0x	30	1	This	this	DT
cord-342785-55r01n0x	30	2	software	software	NN
cord-342785-55r01n0x	30	3	asks	ask	VBZ
cord-342785-55r01n0x	30	4	the	the	DT
cord-342785-55r01n0x	30	5	user	user	NN
cord-342785-55r01n0x	30	6	to	to	TO
cord-342785-55r01n0x	30	7	upload	upload	VB
cord-342785-55r01n0x	30	8	a	a	DT
cord-342785-55r01n0x	30	9	DNA	dna	NN
cord-342785-55r01n0x	30	10	sequence	sequence	NN
cord-342785-55r01n0x	30	11	file	file	NN
cord-342785-55r01n0x	30	12	,	,	,
cord-342785-55r01n0x	30	13	and	and	CC
cord-342785-55r01n0x	30	14	then	then	RB
cord-342785-55r01n0x	30	15	finds	find	VBZ
cord-342785-55r01n0x	30	16	possible	possible	JJ
cord-342785-55r01n0x	30	17	primer	primer	NN
cord-342785-55r01n0x	30	18	/	/	SYM
cord-342785-55r01n0x	30	19	probe	probe	NN
cord-342785-55r01n0x	30	20	sets	set	NNS
cord-342785-55r01n0x	30	21	that	that	WDT
cord-342785-55r01n0x	30	22	meet	meet	VBP
cord-342785-55r01n0x	30	23	the	the	DT
cord-342785-55r01n0x	30	24	assay	assay	NN
cord-342785-55r01n0x	30	25	criteria	criterion	NNS
cord-342785-55r01n0x	30	26	.	.	.
cord-342785-55r01n0x	31	1	Generally	generally	RB
cord-342785-55r01n0x	31	2	a	a	DT
cord-342785-55r01n0x	31	3	researcher	researcher	NN
cord-342785-55r01n0x	31	4	will	will	MD
cord-342785-55r01n0x	31	5	provide	provide	VB
cord-342785-55r01n0x	31	6	as	as	IN
cord-342785-55r01n0x	31	7	input	input	NN
cord-342785-55r01n0x	31	8	a	a	DT
cord-342785-55r01n0x	31	9	gene	gene	NN
cord-342785-55r01n0x	31	10	region	region	NN
cord-342785-55r01n0x	31	11	conserved	conserve	VBN
cord-342785-55r01n0x	31	12	throughout	throughout	IN
cord-342785-55r01n0x	31	13	the	the	DT
cord-342785-55r01n0x	31	14	taxa	taxon	NNS
cord-342785-55r01n0x	31	15	that	that	IN
cord-342785-55r01n0x	31	16	the	the	DT
cord-342785-55r01n0x	31	17	assay	assay	NN
cord-342785-55r01n0x	31	18	is	be	VBZ
cord-342785-55r01n0x	31	19	being	be	VBG
cord-342785-55r01n0x	31	20	designed	design	VBN
cord-342785-55r01n0x	31	21	to	to	TO
cord-342785-55r01n0x	31	22	detect	detect	VB
cord-342785-55r01n0x	31	23	.	.	.
cord-342785-55r01n0x	32	1	The	the	DT
cord-342785-55r01n0x	32	2	software	software	NN
cord-342785-55r01n0x	32	3	then	then	RB
cord-342785-55r01n0x	32	4	provides	provide	VBZ
cord-342785-55r01n0x	32	5	possible	possible	JJ
cord-342785-55r01n0x	32	6	primer	primer	NN
cord-342785-55r01n0x	32	7	/	/	SYM
cord-342785-55r01n0x	32	8	probe	probe	NN
cord-342785-55r01n0x	32	9	sets	set	NNS
cord-342785-55r01n0x	32	10	.	.	.
cord-342785-55r01n0x	33	1	The	the	DT
cord-342785-55r01n0x	33	2	researcher	researcher	NN
cord-342785-55r01n0x	33	3	chooses	choose	VBZ
cord-342785-55r01n0x	33	4	a	a	DT
cord-342785-55r01n0x	33	5	representative	representative	JJ
cord-342785-55r01n0x	33	6	signature	signature	NN
cord-342785-55r01n0x	33	7	.	.	.
cord-342785-55r01n0x	34	1	If	if	IN
cord-342785-55r01n0x	34	2	there	there	EX
cord-342785-55r01n0x	34	3	are	be	VBP
cord-342785-55r01n0x	34	4	single	single	JJ
cord-342785-55r01n0x	34	5	nucleotide	nucleotide	JJ
cord-342785-55r01n0x	34	6	polymorphisms	polymorphism	NNS
cord-342785-55r01n0x	34	7	(	(	-LRB-
cord-342785-55r01n0x	34	8	SNPs	SNPs	NNP
cord-342785-55r01n0x	34	9	)	)	-RRB-
cord-342785-55r01n0x	34	10	within	within	IN
cord-342785-55r01n0x	34	11	the	the	DT
cord-342785-55r01n0x	34	12	chosen	choose	VBN
cord-342785-55r01n0x	34	13	conserved	conserved	JJ
cord-342785-55r01n0x	34	14	region	region	NN
cord-342785-55r01n0x	34	15	,	,	,
cord-342785-55r01n0x	34	16	a	a	DT
cord-342785-55r01n0x	34	17	signature	signature	NN
cord-342785-55r01n0x	34	18	with	with	IN
cord-342785-55r01n0x	34	19	consensus	consensus	NN
cord-342785-55r01n0x	34	20	primers	primer	NNS
cord-342785-55r01n0x	34	21	and	and	CC
cord-342785-55r01n0x	34	22	probes	probe	NNS
cord-342785-55r01n0x	34	23	is	be	VBZ
cord-342785-55r01n0x	34	24	often	often	RB
cord-342785-55r01n0x	34	25	chosen	choose	VBN
cord-342785-55r01n0x	34	26	.	.	.
cord-342785-55r01n0x	35	1	Next	next	RB
cord-342785-55r01n0x	35	2	a	a	DT
cord-342785-55r01n0x	35	3	BLAST	blast	NN
cord-342785-55r01n0x	35	4	[	[	-LRB-
cord-342785-55r01n0x	35	5	2	2	CD
cord-342785-55r01n0x	35	6	]	]	-RRB-
cord-342785-55r01n0x	35	7	search	search	NN
cord-342785-55r01n0x	35	8	is	be	VBZ
cord-342785-55r01n0x	35	9	performed	perform	VBN
cord-342785-55r01n0x	35	10	to	to	TO
cord-342785-55r01n0x	35	11	ensure	ensure	VB
cord-342785-55r01n0x	35	12	that	that	IN
cord-342785-55r01n0x	35	13	the	the	DT
cord-342785-55r01n0x	35	14	primers	primer	NNS
cord-342785-55r01n0x	35	15	are	be	VBP
cord-342785-55r01n0x	35	16	not	not	RB
cord-342785-55r01n0x	35	17	hitting	hit	VBG
cord-342785-55r01n0x	35	18	other	other	JJ
cord-342785-55r01n0x	35	19	targets	target	NNS
cord-342785-55r01n0x	35	20	.	.	.
cord-342785-55r01n0x	36	1	Finally	finally	RB
cord-342785-55r01n0x	36	2	the	the	DT
cord-342785-55r01n0x	36	3	signature	signature	NN
cord-342785-55r01n0x	36	4	is	be	VBZ
cord-342785-55r01n0x	36	5	verified	verify	VBN
cord-342785-55r01n0x	36	6	in	in	IN
cord-342785-55r01n0x	36	7	vitro	vitro	FW
cord-342785-55r01n0x	36	8	with	with	IN
cord-342785-55r01n0x	36	9	laboratory	laboratory	NN
cord-342785-55r01n0x	36	10	strains	strain	NNS
cord-342785-55r01n0x	36	11	.	.	.
cord-342785-55r01n0x	37	1	While	while	IN
cord-342785-55r01n0x	37	2	this	this	DT
cord-342785-55r01n0x	37	3	design	design	NN
cord-342785-55r01n0x	37	4	approach	approach	NN
cord-342785-55r01n0x	37	5	may	may	MD
cord-342785-55r01n0x	37	6	work	work	VB
cord-342785-55r01n0x	37	7	acceptably	acceptably	RB
cord-342785-55r01n0x	37	8	well	well	RB
cord-342785-55r01n0x	37	9	in	in	IN
cord-342785-55r01n0x	37	10	the	the	DT
cord-342785-55r01n0x	37	11	research	research	NN
cord-342785-55r01n0x	37	12	laboratory	laboratory	NN
cord-342785-55r01n0x	37	13	,	,	,
cord-342785-55r01n0x	37	14	the	the	DT
cord-342785-55r01n0x	37	15	clinical	clinical	JJ
cord-342785-55r01n0x	37	16	laboratory	laboratory	NN
cord-342785-55r01n0x	37	17	calls	call	VBZ
cord-342785-55r01n0x	37	18	for	for	IN
cord-342785-55r01n0x	37	19	a	a	DT
cord-342785-55r01n0x	37	20	more	more	RBR
cord-342785-55r01n0x	37	21	thorough	thorough	JJ
cord-342785-55r01n0x	37	22	analysis	analysis	NN
cord-342785-55r01n0x	37	23	to	to	TO
cord-342785-55r01n0x	37	24	ensure	ensure	VB
cord-342785-55r01n0x	37	25	detection	detection	NN
cord-342785-55r01n0x	37	26	of	of	IN
cord-342785-55r01n0x	37	27	novel	novel	NN
cord-342785-55r01n0x	37	28	,	,	,
cord-342785-55r01n0x	37	29	diverse	diverse	JJ
cord-342785-55r01n0x	37	30	,	,	,
cord-342785-55r01n0x	37	31	and	and	CC
cord-342785-55r01n0x	37	32	uncommon	uncommon	JJ
cord-342785-55r01n0x	37	33	strains	strain	NNS
cord-342785-55r01n0x	37	34	.	.	.
cord-342785-55r01n0x	38	1	These	these	DT
cord-342785-55r01n0x	38	2	may	may	MD
cord-342785-55r01n0x	38	3	appear	appear	VB
cord-342785-55r01n0x	38	4	,	,	,
cord-342785-55r01n0x	38	5	for	for	IN
cord-342785-55r01n0x	38	6	example	example	NN
cord-342785-55r01n0x	38	7	,	,	,
cord-342785-55r01n0x	38	8	as	as	IN
cord-342785-55r01n0x	38	9	a	a	DT
cord-342785-55r01n0x	38	10	result	result	NN
cord-342785-55r01n0x	38	11	of	of	IN
cord-342785-55r01n0x	38	12	spread	spread	NN
cord-342785-55r01n0x	38	13	by	by	IN
cord-342785-55r01n0x	38	14	foreign	foreign	JJ
cord-342785-55r01n0x	38	15	travel	travel	NN
cord-342785-55r01n0x	38	16	or	or	CC
cord-342785-55r01n0x	38	17	migration	migration	NN
cord-342785-55r01n0x	38	18	.	.	.
cord-342785-55r01n0x	39	1	Whole	whole	JJ
cord-342785-55r01n0x	39	2	genome	genome	NN
cord-342785-55r01n0x	39	3	based	base	VBN
cord-342785-55r01n0x	39	4	automated	automate	VBN
cord-342785-55r01n0x	39	5	signature	signature	NN
cord-342785-55r01n0x	39	6	design	design	NN
cord-342785-55r01n0x	39	7	[	[	-LRB-
cord-342785-55r01n0x	39	8	3	3	CD
cord-342785-55r01n0x	39	9	]	]	-RRB-
cord-342785-55r01n0x	39	10	presents	present	VBZ
cord-342785-55r01n0x	39	11	a	a	DT
cord-342785-55r01n0x	39	12	great	great	JJ
cord-342785-55r01n0x	39	13	improvement	improvement	NN
cord-342785-55r01n0x	39	14	to	to	IN
cord-342785-55r01n0x	39	15	the	the	DT
cord-342785-55r01n0x	39	16	common	common	JJ
cord-342785-55r01n0x	39	17	method	method	NN
cord-342785-55r01n0x	39	18	.	.	.
cord-342785-55r01n0x	40	1	However	however	RB
cord-342785-55r01n0x	40	2	,	,	,
cord-342785-55r01n0x	40	3	in	in	IN
cord-342785-55r01n0x	40	4	addition	addition	NN
cord-342785-55r01n0x	40	5	to	to	IN
cord-342785-55r01n0x	40	6	better	well	JJR
cord-342785-55r01n0x	40	7	design	design	NN
cord-342785-55r01n0x	40	8	strategies	strategy	NNS
cord-342785-55r01n0x	40	9	,	,	,
cord-342785-55r01n0x	40	10	methods	method	NNS
cord-342785-55r01n0x	40	11	for	for	IN
cord-342785-55r01n0x	40	12	automated	automate	VBN
cord-342785-55r01n0x	40	13	signature	signature	NN
cord-342785-55r01n0x	40	14	evaluation	evaluation	NN
cord-342785-55r01n0x	40	15	are	be	VBP
cord-342785-55r01n0x	40	16	needed	need	VBN
cord-342785-55r01n0x	40	17	.	.	.
cord-342785-55r01n0x	41	1	As	as	IN
cord-342785-55r01n0x	41	2	additional	additional	JJ
cord-342785-55r01n0x	41	3	sequence	sequence	NN
cord-342785-55r01n0x	41	4	data	data	NN
cord-342785-55r01n0x	41	5	becomes	become	VBZ
cord-342785-55r01n0x	41	6	available	available	JJ
cord-342785-55r01n0x	41	7	,	,	,
cord-342785-55r01n0x	41	8	it	-PRON-	PRP
cord-342785-55r01n0x	41	9	is	be	VBZ
cord-342785-55r01n0x	41	10	necessary	necessary	JJ
cord-342785-55r01n0x	41	11	to	to	TO
cord-342785-55r01n0x	41	12	regularly	regularly	RB
cord-342785-55r01n0x	41	13	reassess	reassess	VB
cord-342785-55r01n0x	41	14	the	the	DT
cord-342785-55r01n0x	41	15	predicted	predict	VBN
cord-342785-55r01n0x	41	16	efficacy	efficacy	NN
cord-342785-55r01n0x	41	17	of	of	IN
cord-342785-55r01n0x	41	18	a	a	DT
cord-342785-55r01n0x	41	19	given	give	VBN
cord-342785-55r01n0x	41	20	signature	signature	NN
cord-342785-55r01n0x	41	21	.	.	.
cord-342785-55r01n0x	42	1	This	this	DT
cord-342785-55r01n0x	42	2	analysis	analysis	NN
cord-342785-55r01n0x	42	3	must	must	MD
cord-342785-55r01n0x	42	4	include	include	VB
cord-342785-55r01n0x	42	5	the	the	DT
cord-342785-55r01n0x	42	6	predicted	predict	VBN
cord-342785-55r01n0x	42	7	false	false	JJ
cord-342785-55r01n0x	42	8	negative	negative	JJ
cord-342785-55r01n0x	42	9	and	and	CC
cord-342785-55r01n0x	42	10	false	false	JJ
cord-342785-55r01n0x	42	11	positive	positive	JJ
cord-342785-55r01n0x	42	12	rates	rate	NNS
cord-342785-55r01n0x	42	13	for	for	IN
cord-342785-55r01n0x	42	14	the	the	DT
cord-342785-55r01n0x	42	15	developed	develop	VBN
cord-342785-55r01n0x	42	16	signatures	signature	NNS
cord-342785-55r01n0x	42	17	,	,	,
cord-342785-55r01n0x	42	18	and	and	CC
cord-342785-55r01n0x	42	19	consider	consider	VB
cord-342785-55r01n0x	42	20	all	all	DT
cord-342785-55r01n0x	42	21	available	available	JJ
cord-342785-55r01n0x	42	22	public	public	JJ
cord-342785-55r01n0x	42	23	sequence	sequence	NN
cord-342785-55r01n0x	42	24	data	datum	NNS
cord-342785-55r01n0x	42	25	.	.	.
cord-342785-55r01n0x	43	1	We	-PRON-	PRP
cord-342785-55r01n0x	43	2	have	have	VBP
cord-342785-55r01n0x	43	3	analyzed	analyze	VBN
cord-342785-55r01n0x	43	4	a	a	DT
cord-342785-55r01n0x	43	5	number	number	NN
cord-342785-55r01n0x	43	6	of	of	IN
cord-342785-55r01n0x	43	7	real	real	JJ
cord-342785-55r01n0x	43	8	-	-	HYPH
cord-342785-55r01n0x	43	9	time	time	NN
cord-342785-55r01n0x	43	10	PCR	PCR	NNP
cord-342785-55r01n0x	43	11	assays	assay	NNS
cord-342785-55r01n0x	43	12	found	find	VBN
cord-342785-55r01n0x	43	13	in	in	IN
cord-342785-55r01n0x	43	14	the	the	DT
cord-342785-55r01n0x	43	15	literature	literature	NN
cord-342785-55r01n0x	43	16	based	base	VBN
cord-342785-55r01n0x	43	17	on	on	IN
cord-342785-55r01n0x	43	18	public	public	JJ
cord-342785-55r01n0x	43	19	sequence	sequence	NN
cord-342785-55r01n0x	43	20	data	datum	NNS
cord-342785-55r01n0x	43	21	.	.	.
cord-342785-55r01n0x	44	1	Herein	herein	RB
cord-342785-55r01n0x	44	2	we	-PRON-	PRP
cord-342785-55r01n0x	44	3	report	report	VBP
cord-342785-55r01n0x	44	4	how	how	WRB
cord-342785-55r01n0x	44	5	well	well	RB
cord-342785-55r01n0x	44	6	these	these	DT
cord-342785-55r01n0x	44	7	signatures	signature	NNS
cord-342785-55r01n0x	44	8	performed	perform	VBN
cord-342785-55r01n0x	44	9	,	,	,
cord-342785-55r01n0x	44	10	offer	offer	VBP
cord-342785-55r01n0x	44	11	a	a	DT
cord-342785-55r01n0x	44	12	revised	revise	VBN
cord-342785-55r01n0x	44	13	approach	approach	NN
cord-342785-55r01n0x	44	14	to	to	IN
cord-342785-55r01n0x	44	15	PCR	PCR	NNP
cord-342785-55r01n0x	44	16	assay	assay	NN
cord-342785-55r01n0x	44	17	design	design	NN
cord-342785-55r01n0x	44	18	,	,	,
cord-342785-55r01n0x	44	19	and	and	CC
cord-342785-55r01n0x	44	20	use	use	VB
cord-342785-55r01n0x	44	21	this	this	DT
cord-342785-55r01n0x	44	22	approach	approach	NN
cord-342785-55r01n0x	44	23	to	to	TO
cord-342785-55r01n0x	44	24	produce	produce	VB
cord-342785-55r01n0x	44	25	new	new	JJ
cord-342785-55r01n0x	44	26	assays	assay	NNS
cord-342785-55r01n0x	44	27	predicted	predict	VBN
cord-342785-55r01n0x	44	28	to	to	TO
cord-342785-55r01n0x	44	29	have	have	VB
cord-342785-55r01n0x	44	30	higher	high	JJR
cord-342785-55r01n0x	44	31	sensitivity	sensitivity	NN
cord-342785-55r01n0x	44	32	and	and	CC
cord-342785-55r01n0x	44	33	specificity	specificity	NN
cord-342785-55r01n0x	44	34	.	.	.
cord-342785-55r01n0x	45	1	The	the	DT
cord-342785-55r01n0x	45	2	literature	literature	NN
cord-342785-55r01n0x	45	3	was	be	VBD
cord-342785-55r01n0x	45	4	combed	comb	VBN
cord-342785-55r01n0x	45	5	for	for	IN
cord-342785-55r01n0x	45	6	recently	recently	RB
cord-342785-55r01n0x	45	7	published	publish	VBN
cord-342785-55r01n0x	45	8	articles	article	NNS
cord-342785-55r01n0x	45	9	reporting	report	VBG
cord-342785-55r01n0x	45	10	real	real	JJ
cord-342785-55r01n0x	45	11	-	-	HYPH
cord-342785-55r01n0x	45	12	time	time	NN
cord-342785-55r01n0x	45	13	PCR	PCR	NNP
cord-342785-55r01n0x	45	14	assays	assay	NNS
cord-342785-55r01n0x	45	15	for	for	IN
cord-342785-55r01n0x	45	16	the	the	DT
cord-342785-55r01n0x	45	17	clinical	clinical	JJ
cord-342785-55r01n0x	45	18	detection	detection	NN
cord-342785-55r01n0x	45	19	of	of	IN
cord-342785-55r01n0x	45	20	bacterial	bacterial	JJ
cord-342785-55r01n0x	45	21	and	and	CC
cord-342785-55r01n0x	45	22	viral	viral	JJ
cord-342785-55r01n0x	45	23	taxa	taxon	NNS
cord-342785-55r01n0x	45	24	.	.	.
cord-342785-55r01n0x	46	1	The	the	DT
cord-342785-55r01n0x	46	2	primer	primer	NN
cord-342785-55r01n0x	46	3	and	and	CC
cord-342785-55r01n0x	46	4	probe	probe	NN
cord-342785-55r01n0x	46	5	sequences	sequence	NNS
cord-342785-55r01n0x	46	6	were	be	VBD
cord-342785-55r01n0x	46	7	accumulated	accumulate	VBN
cord-342785-55r01n0x	46	8	,	,	,
cord-342785-55r01n0x	46	9	with	with	IN
cord-342785-55r01n0x	46	10	a	a	DT
cord-342785-55r01n0x	46	11	preference	preference	NN
cord-342785-55r01n0x	46	12	for	for	IN
cord-342785-55r01n0x	46	13	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	46	14	assays	assay	NNS
cord-342785-55r01n0x	46	15	.	.	.
cord-342785-55r01n0x	47	1	However	however	RB
cord-342785-55r01n0x	47	2	,	,	,
cord-342785-55r01n0x	47	3	3	3	CD
cord-342785-55r01n0x	47	4	intercalating	intercalating	NN
cord-342785-55r01n0x	47	5	dye	dye	NN
cord-342785-55r01n0x	47	6	assays	assay	NNS
cord-342785-55r01n0x	47	7	were	be	VBD
cord-342785-55r01n0x	47	8	also	also	RB
cord-342785-55r01n0x	47	9	selected	select	VBN
cord-342785-55r01n0x	47	10	.	.	.
cord-342785-55r01n0x	48	1	Papers	paper	NNS
cord-342785-55r01n0x	48	2	reporting	report	VBG
cord-342785-55r01n0x	48	3	nucleotide	nucleotide	JJ
cord-342785-55r01n0x	48	4	sequences	sequence	NNS
cord-342785-55r01n0x	48	5	that	that	WDT
cord-342785-55r01n0x	48	6	could	could	MD
cord-342785-55r01n0x	48	7	not	not	RB
cord-342785-55r01n0x	48	8	eas	eas	VB
cord-342785-55r01n0x	48	9	-	-	HYPH
cord-342785-55r01n0x	48	10	ily	ily	NNP
cord-342785-55r01n0x	48	11	be	be	VB
cord-342785-55r01n0x	48	12	copied	copy	VBN
cord-342785-55r01n0x	48	13	from	from	IN
cord-342785-55r01n0x	48	14	an	an	DT
cord-342785-55r01n0x	48	15	online	online	JJ
cord-342785-55r01n0x	48	16	source	source	NN
cord-342785-55r01n0x	48	17	were	be	VBD
cord-342785-55r01n0x	48	18	avoided	avoid	VBN
cord-342785-55r01n0x	48	19	.	.	.
cord-342785-55r01n0x	49	1	In	in	IN
cord-342785-55r01n0x	49	2	total	total	NN
cord-342785-55r01n0x	49	3	,	,	,
cord-342785-55r01n0x	49	4	112	112	CD
cord-342785-55r01n0x	49	5	signatures	signature	NNS
cord-342785-55r01n0x	49	6	from	from	IN
cord-342785-55r01n0x	49	7	32	32	CD
cord-342785-55r01n0x	49	8	papers	paper	NNS
cord-342785-55r01n0x	49	9	were	be	VBD
cord-342785-55r01n0x	49	10	analyzed	analyze	VBN
cord-342785-55r01n0x	49	11	.	.	.
cord-342785-55r01n0x	50	1	Local	local	JJ
cord-342785-55r01n0x	50	2	Oracle	Oracle	NNP
cord-342785-55r01n0x	50	3	databases	database	NNS
cord-342785-55r01n0x	50	4	have	have	VBP
cord-342785-55r01n0x	50	5	been	be	VBN
cord-342785-55r01n0x	50	6	constructed	construct	VBN
cord-342785-55r01n0x	50	7	from	from	IN
cord-342785-55r01n0x	50	8	the	the	DT
cord-342785-55r01n0x	50	9	complete	complete	JJ
cord-342785-55r01n0x	50	10	genome	genome	NN
cord-342785-55r01n0x	50	11	sequence	sequence	NN
cord-342785-55r01n0x	50	12	data	datum	NNS
cord-342785-55r01n0x	50	13	available	available	JJ
cord-342785-55r01n0x	50	14	at	at	IN
cord-342785-55r01n0x	50	15	NCBI	NCBI	NNP
cord-342785-55r01n0x	50	16	Genbank	Genbank	NNP
cord-342785-55r01n0x	50	17	,	,	,
cord-342785-55r01n0x	50	18	TIGR	TIGR	NNP
cord-342785-55r01n0x	50	19	,	,	,
cord-342785-55r01n0x	50	20	EMBL	EMBL	NNP
cord-342785-55r01n0x	50	21	,	,	,
cord-342785-55r01n0x	50	22	IMG	IMG	NNP
cord-342785-55r01n0x	50	23	(	(	-LRB-
cord-342785-55r01n0x	50	24	JGI	JGI	NNP
cord-342785-55r01n0x	50	25	)	)	-RRB-
cord-342785-55r01n0x	50	26	,	,	,
cord-342785-55r01n0x	50	27	and	and	CC
cord-342785-55r01n0x	50	28	Baylor	Baylor	NNP
cord-342785-55r01n0x	50	29	HGSC	HGSC	NNP
cord-342785-55r01n0x	50	30	.	.	.
cord-342785-55r01n0x	51	1	We	-PRON-	PRP
cord-342785-55r01n0x	51	2	used	use	VBD
cord-342785-55r01n0x	51	3	our	-PRON-	PRP$
cord-342785-55r01n0x	51	4	"	"	``
cord-342785-55r01n0x	51	5	all_virus	all_virus	NNP
cord-342785-55r01n0x	51	6	"	"	''
cord-342785-55r01n0x	51	7	and	and	CC
cord-342785-55r01n0x	51	8	"	"	``
cord-342785-55r01n0x	51	9	all_bacteria	all_bacteria	NNP
cord-342785-55r01n0x	51	10	"	"	''
cord-342785-55r01n0x	51	11	databases	database	NNS
cord-342785-55r01n0x	51	12	to	to	TO
cord-342785-55r01n0x	51	13	find	find	VB
cord-342785-55r01n0x	51	14	signature	signature	NN
cord-342785-55r01n0x	51	15	matches	match	NNS
cord-342785-55r01n0x	51	16	and	and	CC
cord-342785-55r01n0x	51	17	predict	predict	VB
cord-342785-55r01n0x	51	18	false	false	JJ
cord-342785-55r01n0x	51	19	negatives	negative	NNS
cord-342785-55r01n0x	51	20	and	and	CC
cord-342785-55r01n0x	51	21	false	false	JJ
cord-342785-55r01n0x	51	22	positives	positive	NNS
cord-342785-55r01n0x	51	23	.	.	.
cord-342785-55r01n0x	52	1	These	these	DT
cord-342785-55r01n0x	52	2	databases	database	NNS
cord-342785-55r01n0x	52	3	were	be	VBD
cord-342785-55r01n0x	52	4	designed	design	VBN
cord-342785-55r01n0x	52	5	to	to	TO
cord-342785-55r01n0x	52	6	contain	contain	VB
cord-342785-55r01n0x	52	7	only	only	RB
cord-342785-55r01n0x	52	8	whole	whole	JJ
cord-342785-55r01n0x	52	9	genomes	genome	NNS
cord-342785-55r01n0x	52	10	and	and	CC
cord-342785-55r01n0x	52	11	whole	whole	JJ
cord-342785-55r01n0x	52	12	segments	segment	NNS
cord-342785-55r01n0x	52	13	from	from	IN
cord-342785-55r01n0x	52	14	segmented	segmented	JJ
cord-342785-55r01n0x	52	15	genomes	genome	NNS
cord-342785-55r01n0x	52	16	.	.	.
cord-342785-55r01n0x	53	1	However	however	RB
cord-342785-55r01n0x	53	2	,	,	,
cord-342785-55r01n0x	53	3	the	the	DT
cord-342785-55r01n0x	53	4	heuristics	heuristic	NNS
cord-342785-55r01n0x	53	5	used	use	VBD
cord-342785-55r01n0x	53	6	to	to	TO
cord-342785-55r01n0x	53	7	separate	separate	VB
cord-342785-55r01n0x	53	8	whole	whole	JJ
cord-342785-55r01n0x	53	9	genomes	genome	NNS
cord-342785-55r01n0x	53	10	from	from	IN
cord-342785-55r01n0x	53	11	partial	partial	JJ
cord-342785-55r01n0x	53	12	sequences	sequence	NNS
cord-342785-55r01n0x	53	13	are	be	VBP
cord-342785-55r01n0x	53	14	not	not	RB
cord-342785-55r01n0x	53	15	fail	fail	VB
cord-342785-55r01n0x	53	16	-	-	HYPH
cord-342785-55r01n0x	53	17	proof	proof	NN
cord-342785-55r01n0x	53	18	due	due	IN
cord-342785-55r01n0x	53	19	to	to	IN
cord-342785-55r01n0x	53	20	inconsistency	inconsistency	NN
cord-342785-55r01n0x	53	21	in	in	IN
cord-342785-55r01n0x	53	22	sequence	sequence	NN
cord-342785-55r01n0x	53	23	annotation	annotation	NN
cord-342785-55r01n0x	53	24	within	within	IN
cord-342785-55r01n0x	53	25	the	the	DT
cord-342785-55r01n0x	53	26	public	public	JJ
cord-342785-55r01n0x	53	27	databases	database	NNS
cord-342785-55r01n0x	53	28	.	.	.
cord-342785-55r01n0x	54	1	Consequently	consequently	RB
cord-342785-55r01n0x	54	2	many	many	JJ
cord-342785-55r01n0x	54	3	sequences	sequence	NNS
cord-342785-55r01n0x	54	4	in	in	IN
cord-342785-55r01n0x	54	5	these	these	DT
cord-342785-55r01n0x	54	6	databases	database	NNS
cord-342785-55r01n0x	54	7	may	may	MD
cord-342785-55r01n0x	54	8	show	show	VB
cord-342785-55r01n0x	54	9	up	up	RP
cord-342785-55r01n0x	54	10	as	as	IN
cord-342785-55r01n0x	54	11	false	false	JJ
cord-342785-55r01n0x	54	12	negatives	negative	NNS
cord-342785-55r01n0x	54	13	when	when	WRB
cord-342785-55r01n0x	54	14	they	-PRON-	PRP
cord-342785-55r01n0x	54	15	are	be	VBP
cord-342785-55r01n0x	54	16	actually	actually	RB
cord-342785-55r01n0x	54	17	just	just	RB
cord-342785-55r01n0x	54	18	a	a	DT
cord-342785-55r01n0x	54	19	section	section	NN
cord-342785-55r01n0x	54	20	or	or	CC
cord-342785-55r01n0x	54	21	segment	segment	NN
cord-342785-55r01n0x	54	22	of	of	IN
cord-342785-55r01n0x	54	23	a	a	DT
cord-342785-55r01n0x	54	24	genome	genome	NN
cord-342785-55r01n0x	54	25	that	that	WDT
cord-342785-55r01n0x	54	26	is	be	VBZ
cord-342785-55r01n0x	54	27	not	not	RB
cord-342785-55r01n0x	54	28	expected	expect	VBN
cord-342785-55r01n0x	54	29	to	to	TO
cord-342785-55r01n0x	54	30	contain	contain	VB
cord-342785-55r01n0x	54	31	the	the	DT
cord-342785-55r01n0x	54	32	signature	signature	NN
cord-342785-55r01n0x	54	33	,	,	,
cord-342785-55r01n0x	54	34	and	and	CC
cord-342785-55r01n0x	54	35	we	-PRON-	PRP
cord-342785-55r01n0x	54	36	manually	manually	RB
cord-342785-55r01n0x	54	37	sorted	sort	VBD
cord-342785-55r01n0x	54	38	these	these	DT
cord-342785-55r01n0x	54	39	sequences	sequence	NNS
cord-342785-55r01n0x	54	40	into	into	IN
cord-342785-55r01n0x	54	41	true	true	JJ
cord-342785-55r01n0x	54	42	or	or	CC
cord-342785-55r01n0x	54	43	false	false	JJ
cord-342785-55r01n0x	54	44	negatives	negative	NNS
cord-342785-55r01n0x	54	45	.	.	.
cord-342785-55r01n0x	55	1	A	a	DT
cord-342785-55r01n0x	55	2	freely	freely	RB
cord-342785-55r01n0x	55	3	available	available	JJ
cord-342785-55r01n0x	55	4	real	real	JJ
cord-342785-55r01n0x	55	5	time	time	NN
cord-342785-55r01n0x	55	6	PCR	PCR	NNP
cord-342785-55r01n0x	55	7	analysis	analysis	NN
cord-342785-55r01n0x	55	8	tool	tool	NN
cord-342785-55r01n0x	55	9	called	call	VBN
cord-342785-55r01n0x	55	10	TaqSim	TaqSim	NNP
cord-342785-55r01n0x	55	11	[	[	-LRB-
cord-342785-55r01n0x	55	12	4	4	CD
cord-342785-55r01n0x	55	13	]	]	-RRB-
cord-342785-55r01n0x	55	14	was	be	VBD
cord-342785-55r01n0x	55	15	used	use	VBN
cord-342785-55r01n0x	55	16	to	to	TO
cord-342785-55r01n0x	55	17	find	find	VB
cord-342785-55r01n0x	55	18	public	public	JJ
cord-342785-55r01n0x	55	19	sequences	sequence	NNS
cord-342785-55r01n0x	55	20	that	that	WDT
cord-342785-55r01n0x	55	21	would	would	MD
cord-342785-55r01n0x	55	22	match	match	VB
cord-342785-55r01n0x	55	23	the	the	DT
cord-342785-55r01n0x	55	24	primer	primer	NN
cord-342785-55r01n0x	55	25	/	/	SYM
cord-342785-55r01n0x	55	26	probe	probe	NN
cord-342785-55r01n0x	55	27	assay	assay	NN
cord-342785-55r01n0x	55	28	in	in	IN
cord-342785-55r01n0x	55	29	question	question	NN
cord-342785-55r01n0x	55	30	.	.	.
cord-342785-55r01n0x	56	1	TaqSim	TaqSim	NNP
cord-342785-55r01n0x	56	2	uses	use	VBZ
cord-342785-55r01n0x	56	3	BLAST	blast	NN
cord-342785-55r01n0x	56	4	searches	search	NNS
cord-342785-55r01n0x	56	5	to	to	TO
cord-342785-55r01n0x	56	6	find	find	VB
cord-342785-55r01n0x	56	7	sequences	sequence	NNS
cord-342785-55r01n0x	56	8	that	that	WDT
cord-342785-55r01n0x	56	9	match	match	VBP
cord-342785-55r01n0x	56	10	both	both	CC
cord-342785-55r01n0x	56	11	forward	forward	RB
cord-342785-55r01n0x	56	12	and	and	CC
cord-342785-55r01n0x	56	13	reverse	reverse	VB
cord-342785-55r01n0x	56	14	primers	primer	NNS
cord-342785-55r01n0x	56	15	and	and	CC
cord-342785-55r01n0x	56	16	probe	probe	NN
cord-342785-55r01n0x	56	17	.	.	.
cord-342785-55r01n0x	57	1	To	to	TO
cord-342785-55r01n0x	57	2	be	be	VB
cord-342785-55r01n0x	57	3	reported	report	VBN
cord-342785-55r01n0x	57	4	as	as	IN
cord-342785-55r01n0x	57	5	a	a	DT
cord-342785-55r01n0x	57	6	"	"	``
cord-342785-55r01n0x	57	7	hit	hit	VBN
cord-342785-55r01n0x	57	8	"	"	''
cord-342785-55r01n0x	57	9	the	the	DT
cord-342785-55r01n0x	57	10	primers	primer	NNS
cord-342785-55r01n0x	57	11	and	and	CC
cord-342785-55r01n0x	57	12	probe	probe	NN
cord-342785-55r01n0x	57	13	must	must	MD
cord-342785-55r01n0x	57	14	match	match	VB
cord-342785-55r01n0x	57	15	in	in	IN
cord-342785-55r01n0x	57	16	the	the	DT
cord-342785-55r01n0x	57	17	required	require	VBN
cord-342785-55r01n0x	57	18	orientations	orientation	NNS
cord-342785-55r01n0x	57	19	relative	relative	JJ
cord-342785-55r01n0x	57	20	to	to	IN
cord-342785-55r01n0x	57	21	one	one	CD
cord-342785-55r01n0x	57	22	another	another	DT
cord-342785-55r01n0x	57	23	and	and	CC
cord-342785-55r01n0x	57	24	the	the	DT
cord-342785-55r01n0x	57	25	primers	primer	NNS
cord-342785-55r01n0x	57	26	must	must	MD
cord-342785-55r01n0x	57	27	be	be	VB
cord-342785-55r01n0x	57	28	in	in	IN
cord-342785-55r01n0x	57	29	sufficiently	sufficiently	RB
cord-342785-55r01n0x	57	30	close	close	JJ
cord-342785-55r01n0x	57	31	proximity	proximity	NN
cord-342785-55r01n0x	57	32	.	.	.
cord-342785-55r01n0x	58	1	The	the	DT
cord-342785-55r01n0x	58	2	forward	forward	JJ
cord-342785-55r01n0x	58	3	/	/	SYM
cord-342785-55r01n0x	58	4	reverse	reverse	NN
cord-342785-55r01n0x	58	5	primers	primer	NNS
cord-342785-55r01n0x	58	6	may	may	MD
cord-342785-55r01n0x	58	7	fall	fall	VB
cord-342785-55r01n0x	58	8	on	on	IN
cord-342785-55r01n0x	58	9	either	either	CC
cord-342785-55r01n0x	58	10	the	the	DT
cord-342785-55r01n0x	58	11	plus	plus	NN
cord-342785-55r01n0x	58	12	or	or	CC
cord-342785-55r01n0x	58	13	minus	minus	CC
cord-342785-55r01n0x	58	14	strand	strand	NN
cord-342785-55r01n0x	58	15	,	,	,
cord-342785-55r01n0x	58	16	so	so	RB
cord-342785-55r01n0x	58	17	long	long	RB
cord-342785-55r01n0x	58	18	as	as	IN
cord-342785-55r01n0x	58	19	the	the	DT
cord-342785-55r01n0x	58	20	orientation	orientation	NN
cord-342785-55r01n0x	58	21	relative	relative	JJ
cord-342785-55r01n0x	58	22	to	to	IN
cord-342785-55r01n0x	58	23	one	one	NN
cord-342785-55r01n0x	58	24	another	another	DT
cord-342785-55r01n0x	58	25	is	be	VBZ
cord-342785-55r01n0x	58	26	appropriate	appropriate	JJ
cord-342785-55r01n0x	58	27	.	.	.
cord-342785-55r01n0x	59	1	There	there	EX
cord-342785-55r01n0x	59	2	may	may	MD
cord-342785-55r01n0x	59	3	not	not	RB
cord-342785-55r01n0x	59	4	be	be	VB
cord-342785-55r01n0x	59	5	mismatches	mismatch	NNS
cord-342785-55r01n0x	59	6	at	at	IN
cord-342785-55r01n0x	59	7	the	the	DT
cord-342785-55r01n0x	59	8	3	3	CD
cord-342785-55r01n0x	59	9	'	'	NN
cord-342785-55r01n0x	59	10	end	end	NN
cord-342785-55r01n0x	59	11	of	of	IN
cord-342785-55r01n0x	59	12	either	either	DT
cord-342785-55r01n0x	59	13	primer	primer	NN
cord-342785-55r01n0x	59	14	.	.	.
cord-342785-55r01n0x	60	1	For	for	IN
cord-342785-55r01n0x	60	2	each	each	DT
cord-342785-55r01n0x	60	3	hit	hit	VBN
cord-342785-55r01n0x	60	4	TaqSim	TaqSim	NNP
cord-342785-55r01n0x	60	5	calculates	calculate	VBZ
cord-342785-55r01n0x	60	6	the	the	DT
cord-342785-55r01n0x	60	7	primer	primer	NN
cord-342785-55r01n0x	60	8	and	and	CC
cord-342785-55r01n0x	60	9	probe	probe	VB
cord-342785-55r01n0x	60	10	melting	melt	VBG
cord-342785-55r01n0x	60	11	temperatures	temperature	NNS
cord-342785-55r01n0x	60	12	as	as	IN
cord-342785-55r01n0x	60	13	bound	bind	VBN
cord-342785-55r01n0x	60	14	to	to	IN
cord-342785-55r01n0x	60	15	the	the	DT
cord-342785-55r01n0x	60	16	candidate	candidate	NN
cord-342785-55r01n0x	60	17	hit	hit	VBD
cord-342785-55r01n0x	60	18	sequence	sequence	NN
cord-342785-55r01n0x	60	19	(	(	-LRB-
cord-342785-55r01n0x	60	20	accounting	account	VBG
cord-342785-55r01n0x	60	21	for	for	IN
cord-342785-55r01n0x	60	22	mismatches	mismatch	NNS
cord-342785-55r01n0x	60	23	)	)	-RRB-
cord-342785-55r01n0x	60	24	based	base	VBN
cord-342785-55r01n0x	60	25	on	on	IN
cord-342785-55r01n0x	60	26	reaction	reaction	NN
cord-342785-55r01n0x	60	27	conditions	condition	NNS
cord-342785-55r01n0x	60	28	(	(	-LRB-
cord-342785-55r01n0x	60	29	reagent	reagent	NN
cord-342785-55r01n0x	60	30	concentrations	concentration	NNS
cord-342785-55r01n0x	60	31	and	and	CC
cord-342785-55r01n0x	60	32	hybridization	hybridization	NN
cord-342785-55r01n0x	60	33	temperature	temperature	NN
cord-342785-55r01n0x	60	34	)	)	-RRB-
cord-342785-55r01n0x	60	35	,	,	,
cord-342785-55r01n0x	60	36	and	and	CC
cord-342785-55r01n0x	60	37	returns	return	NNS
cord-342785-55r01n0x	60	38	sequences	sequence	NNS
cord-342785-55r01n0x	60	39	predicted	predict	VBD
cord-342785-55r01n0x	60	40	to	to	TO
cord-342785-55r01n0x	60	41	be	be	VB
cord-342785-55r01n0x	60	42	amplified	amplify	VBN
cord-342785-55r01n0x	60	43	.	.	.
cord-342785-55r01n0x	61	1	Instead	instead	RB
cord-342785-55r01n0x	61	2	of	of	IN
cord-342785-55r01n0x	61	3	replicating	replicate	VBG
cord-342785-55r01n0x	61	4	the	the	DT
cord-342785-55r01n0x	61	5	various	various	JJ
cord-342785-55r01n0x	61	6	exact	exact	JJ
cord-342785-55r01n0x	61	7	reaction	reaction	NN
cord-342785-55r01n0x	61	8	conditions	condition	NNS
cord-342785-55r01n0x	61	9	reported	report	VBN
cord-342785-55r01n0x	61	10	in	in	IN
cord-342785-55r01n0x	61	11	each	each	DT
cord-342785-55r01n0x	61	12	paper	paper	NN
cord-342785-55r01n0x	61	13	,	,	,
cord-342785-55r01n0x	61	14	very	very	RB
cord-342785-55r01n0x	61	15	lenient	lenient	JJ
cord-342785-55r01n0x	61	16	settings	setting	NNS
cord-342785-55r01n0x	61	17	were	be	VBD
cord-342785-55r01n0x	61	18	applied	apply	VBN
cord-342785-55r01n0x	61	19	in	in	IN
cord-342785-55r01n0x	61	20	all	all	DT
cord-342785-55r01n0x	61	21	cases	case	NNS
cord-342785-55r01n0x	61	22	,	,	,
cord-342785-55r01n0x	61	23	essentially	essentially	RB
cord-342785-55r01n0x	61	24	removing	remove	VBG
cord-342785-55r01n0x	61	25	the	the	DT
cord-342785-55r01n0x	61	26	screen	screen	NN
cord-342785-55r01n0x	61	27	for	for	IN
cord-342785-55r01n0x	61	28	primer	primer	NN
cord-342785-55r01n0x	61	29	/	/	SYM
cord-342785-55r01n0x	61	30	probe	probe	NN
cord-342785-55r01n0x	61	31	vs.	vs.	IN
cord-342785-55r01n0x	61	32	candidate	candidate	NN
cord-342785-55r01n0x	61	33	hit	hit	VBD
cord-342785-55r01n0x	61	34	Tm	Tm	NNP
cord-342785-55r01n0x	61	35	by	by	IN
cord-342785-55r01n0x	61	36	setting	set	VBG
cord-342785-55r01n0x	61	37	this	this	DT
cord-342785-55r01n0x	61	38	threshold	threshold	NN
cord-342785-55r01n0x	61	39	to	to	IN
cord-342785-55r01n0x	61	40	0	0	CD
cord-342785-55r01n0x	61	41	°	°	NN
cord-342785-55r01n0x	61	42	K	k	NN
cord-342785-55r01n0x	61	43	,	,	,
cord-342785-55r01n0x	61	44	and	and	CC
cord-342785-55r01n0x	61	45	instead	instead	RB
cord-342785-55r01n0x	61	46	checking	check	VBG
cord-342785-55r01n0x	61	47	for	for	IN
cord-342785-55r01n0x	61	48	specificity	specificity	NN
cord-342785-55r01n0x	61	49	by	by	IN
cord-342785-55r01n0x	61	50	requiring	require	VBG
cord-342785-55r01n0x	61	51	that	that	IN
cord-342785-55r01n0x	61	52	hits	hit	NNS
cord-342785-55r01n0x	61	53	have	have	VBP
cord-342785-55r01n0x	61	54	fewer	few	JJR
cord-342785-55r01n0x	61	55	than	than	IN
cord-342785-55r01n0x	61	56	3	3	CD
cord-342785-55r01n0x	61	57	mismatches	mismatch	NNS
cord-342785-55r01n0x	61	58	per	per	IN
cord-342785-55r01n0x	61	59	primer	primer	NN
cord-342785-55r01n0x	61	60	or	or	CC
cord-342785-55r01n0x	61	61	probe	probe	NN
cord-342785-55r01n0x	61	62	.	.	.
cord-342785-55r01n0x	62	1	TaqSim	TaqSim	NNP
cord-342785-55r01n0x	62	2	's	's	POS
cord-342785-55r01n0x	62	3	predicted	predict	VBN
cord-342785-55r01n0x	62	4	sequence	sequence	NN
cord-342785-55r01n0x	62	5	hits	hit	NNS
cord-342785-55r01n0x	62	6	were	be	VBD
cord-342785-55r01n0x	62	7	compared	compare	VBN
cord-342785-55r01n0x	62	8	with	with	IN
cord-342785-55r01n0x	62	9	sequences	sequence	NNS
cord-342785-55r01n0x	62	10	listed	list	VBN
cord-342785-55r01n0x	62	11	under	under	IN
cord-342785-55r01n0x	62	12	a	a	DT
cord-342785-55r01n0x	62	13	given	give	VBN
cord-342785-55r01n0x	62	14	set	set	NN
cord-342785-55r01n0x	62	15	of	of	IN
cord-342785-55r01n0x	62	16	NCBI	NCBI	NNP
cord-342785-55r01n0x	62	17	taxonomy	taxonomy	NN
cord-342785-55r01n0x	62	18	tree	tree	NN
cord-342785-55r01n0x	62	19	nodes	node	NNS
cord-342785-55r01n0x	62	20	.	.	.
cord-342785-55r01n0x	63	1	For	for	IN
cord-342785-55r01n0x	63	2	instance	instance	NN
cord-342785-55r01n0x	63	3	,	,	,
cord-342785-55r01n0x	63	4	if	if	IN
cord-342785-55r01n0x	63	5	a	a	DT
cord-342785-55r01n0x	63	6	signature	signature	NN
cord-342785-55r01n0x	63	7	was	be	VBD
cord-342785-55r01n0x	63	8	reported	report	VBN
cord-342785-55r01n0x	63	9	to	to	TO
cord-342785-55r01n0x	63	10	detect	detect	VB
cord-342785-55r01n0x	63	11	Hepatitis	Hepatitis	NNP
cord-342785-55r01n0x	63	12	B	B	NNP
cord-342785-55r01n0x	63	13	,	,	,
cord-342785-55r01n0x	63	14	then	then	RB
cord-342785-55r01n0x	63	15	its	-PRON-	PRP$
cord-342785-55r01n0x	63	16	set	set	NN
cord-342785-55r01n0x	63	17	of	of	IN
cord-342785-55r01n0x	63	18	TaqSim	TaqSim	NNP
cord-342785-55r01n0x	63	19	hits	hit	NNS
cord-342785-55r01n0x	63	20	would	would	MD
cord-342785-55r01n0x	63	21	be	be	VB
cord-342785-55r01n0x	63	22	compared	compare	VBN
cord-342785-55r01n0x	63	23	with	with	IN
cord-342785-55r01n0x	63	24	the	the	DT
cord-342785-55r01n0x	63	25	set	set	NN
cord-342785-55r01n0x	63	26	of	of	IN
cord-342785-55r01n0x	63	27	sequences	sequence	NNS
cord-342785-55r01n0x	63	28	under	under	IN
cord-342785-55r01n0x	63	29	node	node	NN
cord-342785-55r01n0x	63	30	10407	10407	CD
cord-342785-55r01n0x	63	31	,	,	,
cord-342785-55r01n0x	63	32	corresponding	correspond	VBG
cord-342785-55r01n0x	63	33	to	to	IN
cord-342785-55r01n0x	63	34	Hepatitis	Hepatitis	NNP
cord-342785-55r01n0x	63	35	B	B	NNP
cord-342785-55r01n0x	63	36	virus	virus	NN
cord-342785-55r01n0x	63	37	.	.	.
cord-342785-55r01n0x	64	1	Sequences	sequence	NNS
cord-342785-55r01n0x	64	2	in	in	IN
cord-342785-55r01n0x	64	3	both	both	DT
cord-342785-55r01n0x	64	4	sets	set	NNS
cord-342785-55r01n0x	64	5	were	be	VBD
cord-342785-55r01n0x	64	6	considered	consider	VBN
cord-342785-55r01n0x	64	7	true	true	JJ
cord-342785-55r01n0x	64	8	positives	positive	NNS
cord-342785-55r01n0x	64	9	,	,	,
cord-342785-55r01n0x	64	10	sequences	sequence	NNS
cord-342785-55r01n0x	64	11	in	in	IN
cord-342785-55r01n0x	64	12	the	the	DT
cord-342785-55r01n0x	64	13	TaqSim	TaqSim	NNP
cord-342785-55r01n0x	64	14	output	output	NN
cord-342785-55r01n0x	64	15	that	that	WDT
cord-342785-55r01n0x	64	16	were	be	VBD
cord-342785-55r01n0x	64	17	missing	miss	VBG
cord-342785-55r01n0x	64	18	from	from	IN
cord-342785-55r01n0x	64	19	the	the	DT
cord-342785-55r01n0x	64	20	chosen	choose	VBN
cord-342785-55r01n0x	64	21	taxonomy	taxonomy	NN
cord-342785-55r01n0x	64	22	nodes	node	NNS
cord-342785-55r01n0x	64	23	were	be	VBD
cord-342785-55r01n0x	64	24	considered	consider	VBN
cord-342785-55r01n0x	64	25	false	false	JJ
cord-342785-55r01n0x	64	26	positives	positive	NNS
cord-342785-55r01n0x	64	27	,	,	,
cord-342785-55r01n0x	64	28	and	and	CC
cord-342785-55r01n0x	64	29	sequences	sequence	NNS
cord-342785-55r01n0x	64	30	that	that	WDT
cord-342785-55r01n0x	64	31	were	be	VBD
cord-342785-55r01n0x	64	32	in	in	IN
cord-342785-55r01n0x	64	33	the	the	DT
cord-342785-55r01n0x	64	34	taxonomy	taxonomy	NN
cord-342785-55r01n0x	64	35	tree	tree	NN
cord-342785-55r01n0x	64	36	but	but	CC
cord-342785-55r01n0x	64	37	missing	miss	VBG
cord-342785-55r01n0x	64	38	from	from	IN
cord-342785-55r01n0x	64	39	the	the	DT
cord-342785-55r01n0x	64	40	TaqSim	TaqSim	NNP
cord-342785-55r01n0x	64	41	output	output	NN
cord-342785-55r01n0x	64	42	were	be	VBD
cord-342785-55r01n0x	64	43	considered	consider	VBN
cord-342785-55r01n0x	64	44	false	false	JJ
cord-342785-55r01n0x	64	45	negatives	negative	NNS
cord-342785-55r01n0x	64	46	.	.	.
cord-342785-55r01n0x	65	1	Test	test	NN
cord-342785-55r01n0x	65	2	statistics	statistic	NNS
cord-342785-55r01n0x	65	3	such	such	JJ
cord-342785-55r01n0x	65	4	as	as	IN
cord-342785-55r01n0x	65	5	specificity	specificity	NN
cord-342785-55r01n0x	65	6	and	and	CC
cord-342785-55r01n0x	65	7	sensitivity	sensitivity	NN
cord-342785-55r01n0x	65	8	(	(	-LRB-
cord-342785-55r01n0x	65	9	power	power	NN
cord-342785-55r01n0x	65	10	)	)	-RRB-
cord-342785-55r01n0x	65	11	were	be	VBD
cord-342785-55r01n0x	65	12	then	then	RB
cord-342785-55r01n0x	65	13	calculated	calculate	VBN
cord-342785-55r01n0x	65	14	.	.	.
cord-342785-55r01n0x	66	1	In	in	IN
cord-342785-55r01n0x	66	2	this	this	DT
cord-342785-55r01n0x	66	3	paper	paper	NN
cord-342785-55r01n0x	66	4	we	-PRON-	PRP
cord-342785-55r01n0x	66	5	define	define	VBP
cord-342785-55r01n0x	66	6	sensitivity	sensitivity	NN
cord-342785-55r01n0x	66	7	and	and	CC
cord-342785-55r01n0x	66	8	specificity	specificity	NN
cord-342785-55r01n0x	66	9	as	as	IN
cord-342785-55r01n0x	66	10	follows	follow	VBZ
cord-342785-55r01n0x	66	11	:	:	:
cord-342785-55r01n0x	67	1	The	the	DT
cord-342785-55r01n0x	67	2	primary	primary	JJ
cord-342785-55r01n0x	67	3	research	research	NN
cord-342785-55r01n0x	67	4	articles	article	NNS
cord-342785-55r01n0x	67	5	were	be	VBD
cord-342785-55r01n0x	67	6	read	read	VBN
cord-342785-55r01n0x	67	7	carefully	carefully	RB
cord-342785-55r01n0x	67	8	to	to	TO
cord-342785-55r01n0x	67	9	determine	determine	VB
cord-342785-55r01n0x	67	10	what	what	WP
cord-342785-55r01n0x	67	11	the	the	DT
cord-342785-55r01n0x	67	12	authors	author	NNS
cord-342785-55r01n0x	67	13	had	have	VBD
cord-342785-55r01n0x	67	14	designed	design	VBN
cord-342785-55r01n0x	67	15	their	-PRON-	PRP$
cord-342785-55r01n0x	67	16	primers	primer	NNS
cord-342785-55r01n0x	67	17	/	/	SYM
cord-342785-55r01n0x	67	18	probes	probe	NNS
cord-342785-55r01n0x	67	19	to	to	TO
cord-342785-55r01n0x	67	20	detect	detect	VB
cord-342785-55r01n0x	67	21	.	.	.
cord-342785-55r01n0x	68	1	NCBI	NCBI	NNP
cord-342785-55r01n0x	68	2	Taxonomy	Taxonomy	NNP
cord-342785-55r01n0x	68	3	nodes	node	NNS
cord-342785-55r01n0x	68	4	were	be	VBD
cord-342785-55r01n0x	68	5	chosen	choose	VBN
cord-342785-55r01n0x	68	6	to	to	TO
cord-342785-55r01n0x	68	7	represent	represent	VB
cord-342785-55r01n0x	68	8	these	these	DT
cord-342785-55r01n0x	68	9	target	target	NN
cord-342785-55r01n0x	68	10	organisms	organism	NNS
cord-342785-55r01n0x	68	11	.	.	.
cord-342785-55r01n0x	69	1	This	this	DT
cord-342785-55r01n0x	69	2	was	be	VBD
cord-342785-55r01n0x	69	3	not	not	RB
cord-342785-55r01n0x	69	4	a	a	DT
cord-342785-55r01n0x	69	5	trivial	trivial	JJ
cord-342785-55r01n0x	69	6	task	task	NN
cord-342785-55r01n0x	69	7	,	,	,
cord-342785-55r01n0x	69	8	since	since	IN
cord-342785-55r01n0x	69	9	many	many	JJ
cord-342785-55r01n0x	69	10	articles	article	NNS
cord-342785-55r01n0x	69	11	lack	lack	VBP
cord-342785-55r01n0x	69	12	clarity	clarity	NN
cord-342785-55r01n0x	69	13	as	as	IN
cord-342785-55r01n0x	69	14	to	to	IN
cord-342785-55r01n0x	69	15	which	which	WDT
cord-342785-55r01n0x	69	16	taxa	taxon	NNS
cord-342785-55r01n0x	69	17	,	,	,
cord-342785-55r01n0x	69	18	specifically	specifically	RB
cord-342785-55r01n0x	69	19	,	,	,
cord-342785-55r01n0x	69	20	their	-PRON-	PRP$
cord-342785-55r01n0x	69	21	assay	assay	NN
cord-342785-55r01n0x	69	22	should	should	MD
cord-342785-55r01n0x	69	23	detect	detect	VB
cord-342785-55r01n0x	69	24	.	.	.
cord-342785-55r01n0x	70	1	For	for	IN
cord-342785-55r01n0x	70	2	instance	instance	NN
cord-342785-55r01n0x	70	3	,	,	,
cord-342785-55r01n0x	70	4	the	the	DT
cord-342785-55r01n0x	70	5	cytomegalovirus	cytomegalovirus	NN
cord-342785-55r01n0x	70	6	assay	assay	NN
cord-342785-55r01n0x	70	7	did	do	VBD
cord-342785-55r01n0x	70	8	not	not	RB
cord-342785-55r01n0x	70	9	detect	detect	VB
cord-342785-55r01n0x	70	10	all	all	DT
cord-342785-55r01n0x	70	11	sequences	sequence	NNS
cord-342785-55r01n0x	70	12	in	in	IN
cord-342785-55r01n0x	70	13	the	the	DT
cord-342785-55r01n0x	70	14	cytomegalovirus	cytomegalovirus	NN
cord-342785-55r01n0x	70	15	genus	genus	NN
cord-342785-55r01n0x	70	16	(	(	-LRB-
cord-342785-55r01n0x	70	17	taxonomy	taxonomy	NN
cord-342785-55r01n0x	70	18	node	node	NN
cord-342785-55r01n0x	70	19	10358	10358	CD
cord-342785-55r01n0x	70	20	)	)	-RRB-
cord-342785-55r01n0x	70	21	,	,	,
cord-342785-55r01n0x	70	22	but	but	CC
cord-342785-55r01n0x	70	23	rather	rather	RB
cord-342785-55r01n0x	70	24	all	all	DT
cord-342785-55r01n0x	70	25	sequences	sequence	NNS
cord-342785-55r01n0x	70	26	in	in	IN
cord-342785-55r01n0x	70	27	the	the	DT
cord-342785-55r01n0x	70	28	human	human	JJ
cord-342785-55r01n0x	70	29	herpesvirus	herpesvirus	NN
cord-342785-55r01n0x	70	30	5	5	CD
cord-342785-55r01n0x	70	31	species	specie	NNS
cord-342785-55r01n0x	70	32	(	(	-LRB-
cord-342785-55r01n0x	70	33	taxonomy	taxonomy	NN
cord-342785-55r01n0x	70	34	node	node	NN
cord-342785-55r01n0x	70	35	10359	10359	CD
cord-342785-55r01n0x	70	36	)	)	-RRB-
cord-342785-55r01n0x	70	37	.	.	.
cord-342785-55r01n0x	71	1	None	none	NN
cord-342785-55r01n0x	71	2	of	of	IN
cord-342785-55r01n0x	71	3	the	the	DT
cord-342785-55r01n0x	71	4	articles	article	NNS
cord-342785-55r01n0x	71	5	specified	specify	VBD
cord-342785-55r01n0x	71	6	a	a	DT
cord-342785-55r01n0x	71	7	taxonomy	taxonomy	NN
cord-342785-55r01n0x	71	8	node	node	NN
cord-342785-55r01n0x	71	9	for	for	IN
cord-342785-55r01n0x	71	10	their	-PRON-	PRP$
cord-342785-55r01n0x	71	11	signatures	signature	NNS
cord-342785-55r01n0x	71	12	.	.	.
cord-342785-55r01n0x	72	1	Perl	perl	JJ
cord-342785-55r01n0x	72	2	scripting	scripting	NN
cord-342785-55r01n0x	72	3	[	[	-LRB-
cord-342785-55r01n0x	72	4	5	5	CD
cord-342785-55r01n0x	72	5	]	]	-RRB-
cord-342785-55r01n0x	72	6	was	be	VBD
cord-342785-55r01n0x	72	7	used	use	VBN
cord-342785-55r01n0x	72	8	to	to	TO
cord-342785-55r01n0x	72	9	help	help	VB
cord-342785-55r01n0x	72	10	compare	compare	VB
cord-342785-55r01n0x	72	11	BLAST	blast	NN
cord-342785-55r01n0x	72	12	hits	hit	NNS
cord-342785-55r01n0x	72	13	and	and	CC
cord-342785-55r01n0x	72	14	taxonomy	taxonomy	NN
cord-342785-55r01n0x	72	15	node	node	NN
cord-342785-55r01n0x	72	16	sequences	sequence	NNS
cord-342785-55r01n0x	72	17	,	,	,
cord-342785-55r01n0x	72	18	and	and	CC
cord-342785-55r01n0x	72	19	count	count	VB
cord-342785-55r01n0x	72	20	false	false	JJ
cord-342785-55r01n0x	72	21	negative	negative	JJ
cord-342785-55r01n0x	72	22	,	,	,
cord-342785-55r01n0x	72	23	false	false	JJ
cord-342785-55r01n0x	72	24	positive	positive	JJ
cord-342785-55r01n0x	72	25	and	and	CC
cord-342785-55r01n0x	72	26	true	true	JJ
cord-342785-55r01n0x	72	27	positive	positive	JJ
cord-342785-55r01n0x	72	28	sequence	sequence	NN
cord-342785-55r01n0x	72	29	matches	match	NNS
cord-342785-55r01n0x	72	30	.	.	.
cord-342785-55r01n0x	73	1	However	however	RB
cord-342785-55r01n0x	73	2	some	some	DT
cord-342785-55r01n0x	73	3	sequences	sequence	NNS
cord-342785-55r01n0x	73	4	required	require	VBD
cord-342785-55r01n0x	73	5	hand	hand	NN
cord-342785-55r01n0x	73	6	sorting	sorting	NN
cord-342785-55r01n0x	73	7	due	due	IN
cord-342785-55r01n0x	73	8	to	to	IN
cord-342785-55r01n0x	73	9	the	the	DT
cord-342785-55r01n0x	73	10	wide	wide	JJ
cord-342785-55r01n0x	73	11	array	array	NN
cord-342785-55r01n0x	73	12	of	of	IN
cord-342785-55r01n0x	73	13	sequence	sequence	NN
cord-342785-55r01n0x	73	14	types	type	NNS
cord-342785-55r01n0x	73	15	and	and	CC
cord-342785-55r01n0x	73	16	annotations	annotation	NNS
cord-342785-55r01n0x	73	17	.	.	.
cord-342785-55r01n0x	74	1	These	these	DT
cord-342785-55r01n0x	74	2	often	often	RB
cord-342785-55r01n0x	74	3	represented	represent	VBD
cord-342785-55r01n0x	74	4	segmented	segmented	JJ
cord-342785-55r01n0x	74	5	genomes	genome	NNS
cord-342785-55r01n0x	74	6	,	,	,
cord-342785-55r01n0x	74	7	in	in	IN
cord-342785-55r01n0x	74	8	which	which	WDT
cord-342785-55r01n0x	74	9	case	case	NN
cord-342785-55r01n0x	74	10	many	many	JJ
cord-342785-55r01n0x	74	11	of	of	IN
cord-342785-55r01n0x	74	12	the	the	DT
cord-342785-55r01n0x	74	13	would	would	MD
cord-342785-55r01n0x	74	14	-	-	HYPH
cord-342785-55r01n0x	74	15	be	be	VB
cord-342785-55r01n0x	74	16	false	false	JJ
cord-342785-55r01n0x	74	17	negative	negative	JJ
cord-342785-55r01n0x	74	18	sequences	sequence	NNS
cord-342785-55r01n0x	74	19	simply	simply	RB
cord-342785-55r01n0x	74	20	represent	represent	VBP
cord-342785-55r01n0x	74	21	a	a	DT
cord-342785-55r01n0x	74	22	different	different	JJ
cord-342785-55r01n0x	74	23	segment	segment	NN
cord-342785-55r01n0x	74	24	than	than	IN
cord-342785-55r01n0x	74	25	that	that	DT
cord-342785-55r01n0x	74	26	on	on	IN
cord-342785-55r01n0x	74	27	which	which	WDT
cord-342785-55r01n0x	74	28	the	the	DT
cord-342785-55r01n0x	74	29	signature	signature	NN
cord-342785-55r01n0x	74	30	lands	land	NNS
cord-342785-55r01n0x	74	31	,	,	,
cord-342785-55r01n0x	74	32	so	so	RB
cord-342785-55r01n0x	74	33	we	-PRON-	PRP
cord-342785-55r01n0x	74	34	manually	manually	RB
cord-342785-55r01n0x	74	35	tabulated	tabulate	VBD
cord-342785-55r01n0x	74	36	them	-PRON-	PRP
cord-342785-55r01n0x	74	37	as	as	IN
cord-342785-55r01n0x	74	38	true	true	JJ
cord-342785-55r01n0x	74	39	negatives	negative	NNS
cord-342785-55r01n0x	74	40	.	.	.
cord-342785-55r01n0x	75	1	They	-PRON-	PRP
cord-342785-55r01n0x	75	2	may	may	MD
cord-342785-55r01n0x	75	3	also	also	RB
cord-342785-55r01n0x	75	4	represent	represent	VB
cord-342785-55r01n0x	75	5	plasmids	plasmid	NNS
cord-342785-55r01n0x	75	6	.	.	.
cord-342785-55r01n0x	76	1	In	in	IN
cord-342785-55r01n0x	76	2	these	these	DT
cord-342785-55r01n0x	76	3	situations	situation	NNS
cord-342785-55r01n0x	76	4	,	,	,
cord-342785-55r01n0x	76	5	a	a	DT
cord-342785-55r01n0x	76	6	careful	careful	JJ
cord-342785-55r01n0x	76	7	review	review	NN
cord-342785-55r01n0x	76	8	of	of	IN
cord-342785-55r01n0x	76	9	the	the	DT
cord-342785-55r01n0x	76	10	Genbank	Genbank	NNP
cord-342785-55r01n0x	76	11	entry	entry	NN
cord-342785-55r01n0x	76	12	,	,	,
cord-342785-55r01n0x	76	13	and	and	CC
cord-342785-55r01n0x	76	14	sometimes	sometimes	RB
cord-342785-55r01n0x	76	15	of	of	IN
cord-342785-55r01n0x	76	16	the	the	DT
cord-342785-55r01n0x	76	17	primary	primary	JJ
cord-342785-55r01n0x	76	18	article	article	NN
cord-342785-55r01n0x	76	19	cited	cite	VBN
cord-342785-55r01n0x	76	20	by	by	IN
cord-342785-55r01n0x	76	21	Genbank	Genbank	NNP
cord-342785-55r01n0x	76	22	,	,	,
cord-342785-55r01n0x	76	23	was	be	VBD
cord-342785-55r01n0x	76	24	necessary	necessary	JJ
cord-342785-55r01n0x	76	25	to	to	TO
cord-342785-55r01n0x	76	26	determine	determine	VB
cord-342785-55r01n0x	76	27	if	if	IN
cord-342785-55r01n0x	76	28	the	the	DT
cord-342785-55r01n0x	76	29	sequence	sequence	NN
cord-342785-55r01n0x	76	30	of	of	IN
cord-342785-55r01n0x	76	31	interest	interest	NN
cord-342785-55r01n0x	76	32	was	be	VBD
cord-342785-55r01n0x	76	33	truly	truly	RB
cord-342785-55r01n0x	76	34	a	a	DT
cord-342785-55r01n0x	76	35	false	false	JJ
cord-342785-55r01n0x	76	36	negative	negative	NN
cord-342785-55r01n0x	76	37	.	.	.
cord-342785-55r01n0x	77	1	Although	although	IN
cord-342785-55r01n0x	77	2	we	-PRON-	PRP
cord-342785-55r01n0x	77	3	attempt	attempt	VBP
cord-342785-55r01n0x	77	4	to	to	TO
cord-342785-55r01n0x	77	5	include	include	VB
cord-342785-55r01n0x	77	6	only	only	RB
cord-342785-55r01n0x	77	7	complete	complete	JJ
cord-342785-55r01n0x	77	8	genomes	genome	NNS
cord-342785-55r01n0x	77	9	in	in	IN
cord-342785-55r01n0x	77	10	our	-PRON-	PRP$
cord-342785-55r01n0x	77	11	sequence	sequence	NN
cord-342785-55r01n0x	77	12	database	database	NN
cord-342785-55r01n0x	77	13	,	,	,
cord-342785-55r01n0x	77	14	because	because	IN
cord-342785-55r01n0x	77	15	of	of	IN
cord-342785-55r01n0x	77	16	inconsistencies	inconsistency	NNS
cord-342785-55r01n0x	77	17	in	in	IN
cord-342785-55r01n0x	77	18	the	the	DT
cord-342785-55r01n0x	77	19	annotation	annotation	NN
cord-342785-55r01n0x	77	20	of	of	IN
cord-342785-55r01n0x	77	21	sequence	sequence	NN
cord-342785-55r01n0x	77	22	data	datum	NNS
cord-342785-55r01n0x	77	23	some	some	DT
cord-342785-55r01n0x	77	24	partial	partial	JJ
cord-342785-55r01n0x	77	25	sequences	sequence	NNS
cord-342785-55r01n0x	77	26	nevertheless	nevertheless	RB
cord-342785-55r01n0x	77	27	make	make	VBP
cord-342785-55r01n0x	77	28	it	-PRON-	PRP
cord-342785-55r01n0x	77	29	into	into	IN
cord-342785-55r01n0x	77	30	our	-PRON-	PRP$
cord-342785-55r01n0x	77	31	databases	database	NNS
cord-342785-55r01n0x	77	32	.	.	.
cord-342785-55r01n0x	78	1	Any	any	DT
cord-342785-55r01n0x	78	2	of	of	IN
cord-342785-55r01n0x	78	3	these	these	DT
cord-342785-55r01n0x	78	4	partial	partial	JJ
cord-342785-55r01n0x	78	5	CDS	CDS	NNP
cord-342785-55r01n0x	78	6	's	's	POS
cord-342785-55r01n0x	78	7	documented	document	VBN
cord-342785-55r01n0x	78	8	as	as	IN
cord-342785-55r01n0x	78	9	containing	contain	VBG
cord-342785-55r01n0x	78	10	the	the	DT
cord-342785-55r01n0x	78	11	target	target	NN
cord-342785-55r01n0x	78	12	gene	gene	NN
cord-342785-55r01n0x	78	13	on	on	IN
cord-342785-55r01n0x	78	14	which	which	WDT
cord-342785-55r01n0x	78	15	the	the	DT
cord-342785-55r01n0x	78	16	signature	signature	NN
cord-342785-55r01n0x	78	17	was	be	VBD
cord-342785-55r01n0x	78	18	supposed	suppose	VBN
cord-342785-55r01n0x	78	19	to	to	TO
cord-342785-55r01n0x	78	20	land	land	VB
cord-342785-55r01n0x	78	21	were	be	VBD
cord-342785-55r01n0x	78	22	counted	count	VBN
cord-342785-55r01n0x	78	23	as	as	IN
cord-342785-55r01n0x	78	24	false	false	JJ
cord-342785-55r01n0x	78	25	negatives	negative	NNS
cord-342785-55r01n0x	78	26	,	,	,
cord-342785-55r01n0x	78	27	but	but	CC
cord-342785-55r01n0x	78	28	those	those	DT
cord-342785-55r01n0x	78	29	partial	partial	JJ
cord-342785-55r01n0x	78	30	CDS	CDS	NNP
cord-342785-55r01n0x	78	31	's	's	POS
cord-342785-55r01n0x	78	32	not	not	RB
cord-342785-55r01n0x	78	33	documented	document	VBN
cord-342785-55r01n0x	78	34	to	to	TO
cord-342785-55r01n0x	78	35	contain	contain	VB
cord-342785-55r01n0x	78	36	the	the	DT
cord-342785-55r01n0x	78	37	target	target	NN
cord-342785-55r01n0x	78	38	gene	gene	NN
cord-342785-55r01n0x	78	39	were	be	VBD
cord-342785-55r01n0x	78	40	eliminated	eliminate	VBN
cord-342785-55r01n0x	78	41	from	from	IN
cord-342785-55r01n0x	78	42	the	the	DT
cord-342785-55r01n0x	78	43	false	false	JJ
cord-342785-55r01n0x	78	44	negative	negative	JJ
cord-342785-55r01n0x	78	45	pool	pool	NN
cord-342785-55r01n0x	78	46	because	because	IN
cord-342785-55r01n0x	78	47	it	-PRON-	PRP
cord-342785-55r01n0x	78	48	is	be	VBZ
cord-342785-55r01n0x	78	49	possible	possible	JJ
cord-342785-55r01n0x	78	50	the	the	DT
cord-342785-55r01n0x	78	51	signature	signature	NN
cord-342785-55r01n0x	78	52	could	could	MD
cord-342785-55r01n0x	78	53	land	land	VB
cord-342785-55r01n0x	78	54	on	on	IN
cord-342785-55r01n0x	78	55	the	the	DT
cord-342785-55r01n0x	78	56	unsequenced	unsequenced	JJ
cord-342785-55r01n0x	78	57	section	section	NN
cord-342785-55r01n0x	78	58	with	with	IN
cord-342785-55r01n0x	78	59	the	the	DT
cord-342785-55r01n0x	78	60	target	target	NN
cord-342785-55r01n0x	78	61	gene	gene	NN
cord-342785-55r01n0x	78	62	.	.	.
cord-342785-55r01n0x	79	1	Our	-PRON-	PRP$
cord-342785-55r01n0x	79	2	database	database	NN
cord-342785-55r01n0x	79	3	also	also	RB
cord-342785-55r01n0x	79	4	contains	contain	VBZ
cord-342785-55r01n0x	79	5	"	"	``
cord-342785-55r01n0x	79	6	glued	glue	VBN
cord-342785-55r01n0x	79	7	fragments	fragment	NNS
cord-342785-55r01n0x	79	8	"	"	''
cord-342785-55r01n0x	79	9	,	,	,
cord-342785-55r01n0x	79	10	which	which	WDT
cord-342785-55r01n0x	79	11	represent	represent	VBP
cord-342785-55r01n0x	79	12	draft	draft	NN
cord-342785-55r01n0x	79	13	genomes	genome	NNS
cord-342785-55r01n0x	79	14	"	"	``
cord-342785-55r01n0x	79	15	glued	glue	VBN
cord-342785-55r01n0x	79	16	"	"	''
cord-342785-55r01n0x	79	17	together	together	RB
cord-342785-55r01n0x	79	18	with	with	IN
cord-342785-55r01n0x	79	19	hundreds	hundred	NNS
cord-342785-55r01n0x	79	20	of	of	IN
cord-342785-55r01n0x	79	21	"	"	``
cord-342785-55r01n0x	79	22	N"s	N"s	NNP
cord-342785-55r01n0x	79	23	as	as	IN
cord-342785-55r01n0x	79	24	a	a	DT
cord-342785-55r01n0x	79	25	simple	simple	JJ
cord-342785-55r01n0x	79	26	way	way	NN
cord-342785-55r01n0x	79	27	to	to	TO
cord-342785-55r01n0x	79	28	keep	keep	VB
cord-342785-55r01n0x	79	29	the	the	DT
cord-342785-55r01n0x	79	30	separate	separate	JJ
cord-342785-55r01n0x	79	31	contigs	contigs	NN
cord-342785-55r01n0x	79	32	associated	associate	VBN
cord-342785-55r01n0x	79	33	as	as	IN
cord-342785-55r01n0x	79	34	part	part	NN
cord-342785-55r01n0x	79	35	of	of	IN
cord-342785-55r01n0x	79	36	the	the	DT
cord-342785-55r01n0x	79	37	same	same	JJ
cord-342785-55r01n0x	79	38	genome	genome	NN
cord-342785-55r01n0x	79	39	.	.	.
cord-342785-55r01n0x	80	1	While	while	IN
cord-342785-55r01n0x	80	2	we	-PRON-	PRP
cord-342785-55r01n0x	80	3	report	report	VBP
cord-342785-55r01n0x	80	4	false	false	JJ
cord-342785-55r01n0x	80	5	negatives	negative	NNS
cord-342785-55r01n0x	80	6	from	from	IN
cord-342785-55r01n0x	80	7	these	these	DT
cord-342785-55r01n0x	80	8	draft	draft	NN
cord-342785-55r01n0x	80	9	genomes	genome	NNS
cord-342785-55r01n0x	80	10	,	,	,
cord-342785-55r01n0x	80	11	it	-PRON-	PRP
cord-342785-55r01n0x	80	12	is	be	VBZ
cord-342785-55r01n0x	80	13	possible	possible	JJ
cord-342785-55r01n0x	80	14	that	that	IN
cord-342785-55r01n0x	80	15	the	the	DT
cord-342785-55r01n0x	80	16	signatures	signature	NNS
cord-342785-55r01n0x	80	17	could	could	MD
cord-342785-55r01n0x	80	18	land	land	VB
cord-342785-55r01n0x	80	19	on	on	IN
cord-342785-55r01n0x	80	20	gaps	gap	NNS
cord-342785-55r01n0x	80	21	between	between	IN
cord-342785-55r01n0x	80	22	the	the	DT
cord-342785-55r01n0x	80	23	contigs	contigs	NN
cord-342785-55r01n0x	80	24	,	,	,
cord-342785-55r01n0x	80	25	and	and	CC
cord-342785-55r01n0x	80	26	that	that	IN
cord-342785-55r01n0x	80	27	finished	finish	VBD
cord-342785-55r01n0x	80	28	sequencing	sequencing	NN
cord-342785-55r01n0x	80	29	could	could	MD
cord-342785-55r01n0x	80	30	result	result	VB
cord-342785-55r01n0x	80	31	in	in	IN
cord-342785-55r01n0x	80	32	re	re	NN
cord-342785-55r01n0x	80	33	-	-	NN
cord-342785-55r01n0x	80	34	classification	classification	NN
cord-342785-55r01n0x	80	35	as	as	IN
cord-342785-55r01n0x	80	36	a	a	DT
cord-342785-55r01n0x	80	37	true	true	JJ
cord-342785-55r01n0x	80	38	positive	positive	JJ
cord-342785-55r01n0x	80	39	.	.	.
cord-342785-55r01n0x	81	1	Tables	table	NNS
cord-342785-55r01n0x	81	2	1	1	CD
cord-342785-55r01n0x	81	3	,	,	,
cord-342785-55r01n0x	81	4	2	2	CD
cord-342785-55r01n0x	81	5	,	,	,
cord-342785-55r01n0x	81	6	3	3	CD
cord-342785-55r01n0x	81	7	,	,	,
cord-342785-55r01n0x	81	8	4	4	CD
cord-342785-55r01n0x	81	9	,	,	,
cord-342785-55r01n0x	81	10	5	5	CD
cord-342785-55r01n0x	81	11	summarize	summarize	VB
cord-342785-55r01n0x	81	12	our	-PRON-	PRP$
cord-342785-55r01n0x	81	13	analysis	analysis	NN
cord-342785-55r01n0x	81	14	of	of	IN
cord-342785-55r01n0x	81	15	various	various	JJ
cord-342785-55r01n0x	81	16	DNA	dna	NN
cord-342785-55r01n0x	81	17	signatures	signature	NNS
cord-342785-55r01n0x	81	18	.	.	.
cord-342785-55r01n0x	82	1	Details	detail	NNS
cord-342785-55r01n0x	82	2	of	of	IN
cord-342785-55r01n0x	82	3	all	all	DT
cord-342785-55r01n0x	82	4	true	true	JJ
cord-342785-55r01n0x	82	5	positive	positive	JJ
cord-342785-55r01n0x	82	6	,	,	,
cord-342785-55r01n0x	82	7	false	false	JJ
cord-342785-55r01n0x	82	8	positive	positive	JJ
cord-342785-55r01n0x	82	9	and	and	CC
cord-342785-55r01n0x	82	10	false	false	JJ
cord-342785-55r01n0x	82	11	negative	negative	JJ
cord-342785-55r01n0x	82	12	sequences	sequence	NNS
cord-342785-55r01n0x	82	13	are	be	VBP
cord-342785-55r01n0x	82	14	available	available	JJ
cord-342785-55r01n0x	82	15	from	from	IN
cord-342785-55r01n0x	82	16	the	the	DT
cord-342785-55r01n0x	82	17	authors	author	NNS
cord-342785-55r01n0x	82	18	.	.	.
cord-342785-55r01n0x	83	1	Note	note	VB
cord-342785-55r01n0x	83	2	that	that	IN
cord-342785-55r01n0x	83	3	these	these	DT
cord-342785-55r01n0x	83	4	results	result	NNS
cord-342785-55r01n0x	83	5	are	be	VBP
cord-342785-55r01n0x	83	6	in	in	IN
cord-342785-55r01n0x	83	7	silico	silico	NN
cord-342785-55r01n0x	83	8	results	result	NNS
cord-342785-55r01n0x	83	9	;	;	:
cord-342785-55r01n0x	83	10	no	no	DT
cord-342785-55r01n0x	83	11	laboratory	laboratory	NN
cord-342785-55r01n0x	83	12	testing	testing	NN
cord-342785-55r01n0x	83	13	was	be	VBD
cord-342785-55r01n0x	83	14	performed	perform	VBN
cord-342785-55r01n0x	83	15	for	for	IN
cord-342785-55r01n0x	83	16	verification	verification	NN
cord-342785-55r01n0x	83	17	,	,	,
cord-342785-55r01n0x	83	18	so	so	IN
cord-342785-55r01n0x	83	19	that	that	IN
cord-342785-55r01n0x	83	20	by	by	IN
cord-342785-55r01n0x	83	21	stating	state	VBG
cord-342785-55r01n0x	83	22	that	that	IN
cord-342785-55r01n0x	83	23	an	an	DT
cord-342785-55r01n0x	83	24	organism	organism	NN
cord-342785-55r01n0x	83	25	is	be	VBZ
cord-342785-55r01n0x	83	26	"	"	``
cord-342785-55r01n0x	83	27	detected	detect	VBN
cord-342785-55r01n0x	83	28	"	"	''
cord-342785-55r01n0x	83	29	we	-PRON-	PRP
cord-342785-55r01n0x	83	30	mean	mean	VBP
cord-342785-55r01n0x	83	31	that	that	IN
cord-342785-55r01n0x	83	32	this	this	DT
cord-342785-55r01n0x	83	33	is	be	VBZ
cord-342785-55r01n0x	83	34	our	-PRON-	PRP$
cord-342785-55r01n0x	83	35	prediction	prediction	NN
cord-342785-55r01n0x	83	36	based	base	VBN
cord-342785-55r01n0x	83	37	on	on	IN
cord-342785-55r01n0x	83	38	sequence	sequence	NN
cord-342785-55r01n0x	83	39	data	datum	NNS
cord-342785-55r01n0x	83	40	.	.	.
cord-342785-55r01n0x	84	1	A	a	DT
cord-342785-55r01n0x	84	2	few	few	JJ
cord-342785-55r01n0x	84	3	notes	note	NNS
cord-342785-55r01n0x	84	4	of	of	IN
cord-342785-55r01n0x	84	5	interest	interest	NN
cord-342785-55r01n0x	84	6	concerning	concern	VBG
cord-342785-55r01n0x	84	7	the	the	DT
cord-342785-55r01n0x	84	8	data	datum	NNS
cord-342785-55r01n0x	84	9	in	in	IN
cord-342785-55r01n0x	84	10	the	the	DT
cord-342785-55r01n0x	84	11	tables	table	NNS
cord-342785-55r01n0x	84	12	are	be	VBP
cord-342785-55r01n0x	84	13	described	describe	VBN
cord-342785-55r01n0x	84	14	below	below	IN
cord-342785-55r01n0x	84	15	:	:	:
cord-342785-55r01n0x	84	16	The	the	DT
cord-342785-55r01n0x	84	17	two	two	CD
cord-342785-55r01n0x	84	18	human	human	JJ
cord-342785-55r01n0x	84	19	corona	corona	NN
cord-342785-55r01n0x	84	20	virus	virus	NN
cord-342785-55r01n0x	84	21	strains	strain	NNS
cord-342785-55r01n0x	84	22	,	,	,
cord-342785-55r01n0x	84	23	229E	229e	CD
cord-342785-55r01n0x	84	24	and	and	CC
cord-342785-55r01n0x	84	25	OC43	OC43	NNP
cord-342785-55r01n0x	84	26	are	be	VBP
cord-342785-55r01n0x	84	27	a	a	DT
cord-342785-55r01n0x	84	28	frequent	frequent	JJ
cord-342785-55r01n0x	84	29	cause	cause	NN
cord-342785-55r01n0x	84	30	of	of	IN
cord-342785-55r01n0x	84	31	the	the	DT
cord-342785-55r01n0x	84	32	common	common	JJ
cord-342785-55r01n0x	84	33	cold	cold	NN
cord-342785-55r01n0x	84	34	[	[	-LRB-
cord-342785-55r01n0x	84	35	6	6	CD
cord-342785-55r01n0x	84	36	]	]	-RRB-
cord-342785-55r01n0x	84	37	.	.	.
cord-342785-55r01n0x	85	1	A	a	DT
cord-342785-55r01n0x	85	2	Taqman	Taqman	NNP
cord-342785-55r01n0x	85	3	assay	assay	NN
cord-342785-55r01n0x	85	4	for	for	IN
cord-342785-55r01n0x	85	5	229E	229e	CD
cord-342785-55r01n0x	85	6	was	be	VBD
cord-342785-55r01n0x	85	7	predicted	predict	VBN
cord-342785-55r01n0x	85	8	to	to	TO
cord-342785-55r01n0x	85	9	perform	perform	VB
cord-342785-55r01n0x	85	10	perfectly	perfectly	RB
cord-342785-55r01n0x	85	11	,	,	,
cord-342785-55r01n0x	85	12	while	while	IN
cord-342785-55r01n0x	85	13	an	an	DT
cord-342785-55r01n0x	85	14	assay	assay	NN
cord-342785-55r01n0x	85	15	for	for	IN
cord-342785-55r01n0x	85	16	OC43	OC43	NNP
cord-342785-55r01n0x	85	17	turned	turn	VBD
cord-342785-55r01n0x	85	18	up	up	RP
cord-342785-55r01n0x	85	19	a	a	DT
cord-342785-55r01n0x	85	20	number	number	NN
cord-342785-55r01n0x	85	21	of	of	IN
cord-342785-55r01n0x	85	22	false	false	JJ
cord-342785-55r01n0x	85	23	positives	positive	NNS
cord-342785-55r01n0x	85	24	,	,	,
cord-342785-55r01n0x	85	25	all	all	DT
cord-342785-55r01n0x	85	26	of	of	IN
cord-342785-55r01n0x	85	27	which	which	WDT
cord-342785-55r01n0x	85	28	were	be	VBD
cord-342785-55r01n0x	85	29	animal	animal	NN
cord-342785-55r01n0x	85	30	corona	corona	NN
cord-342785-55r01n0x	85	31	viruses	virus	NNS
cord-342785-55r01n0x	85	32	.	.	.
cord-342785-55r01n0x	86	1	A	a	DT
cord-342785-55r01n0x	86	2	coxsackie	coxsackie	NN
cord-342785-55r01n0x	86	3	B3	B3	NNP
cord-342785-55r01n0x	86	4	virus	virus	NN
cord-342785-55r01n0x	86	5	assay	assay	NN
cord-342785-55r01n0x	86	6	[	[	-LRB-
cord-342785-55r01n0x	86	7	7	7	CD
cord-342785-55r01n0x	86	8	]	]	-RRB-
cord-342785-55r01n0x	86	9	performed	perform	VBN
cord-342785-55r01n0x	86	10	well	well	RB
cord-342785-55r01n0x	86	11	,	,	,
cord-342785-55r01n0x	86	12	but	but	CC
cord-342785-55r01n0x	86	13	a	a	DT
cord-342785-55r01n0x	86	14	coxsackie	coxsackie	NN
cord-342785-55r01n0x	86	15	B4	B4	NNP
cord-342785-55r01n0x	86	16	assay	assay	NN
cord-342785-55r01n0x	86	17	[	[	-LRB-
cord-342785-55r01n0x	86	18	8	8	CD
cord-342785-55r01n0x	86	19	]	]	-RRB-
cord-342785-55r01n0x	86	20	hit	hit	VBD
cord-342785-55r01n0x	86	21	many	many	JJ
cord-342785-55r01n0x	86	22	other	other	JJ
cord-342785-55r01n0x	86	23	human	human	JJ
cord-342785-55r01n0x	86	24	coxsackie	coxsackie	NNS
cord-342785-55r01n0x	86	25	,	,	,
cord-342785-55r01n0x	86	26	echo	echo	NNP
cord-342785-55r01n0x	86	27	,	,	,
cord-342785-55r01n0x	86	28	and	and	CC
cord-342785-55r01n0x	86	29	entero	entero	NNP
cord-342785-55r01n0x	86	30	viruses	virus	NNS
cord-342785-55r01n0x	86	31	.	.	.
cord-342785-55r01n0x	87	1	Four	four	CD
cord-342785-55r01n0x	87	2	out	out	IN
cord-342785-55r01n0x	87	3	of	of	IN
cord-342785-55r01n0x	87	4	5	5	CD
cord-342785-55r01n0x	87	5	false	false	JJ
cord-342785-55r01n0x	87	6	negatives	negative	NNS
cord-342785-55r01n0x	87	7	for	for	IN
cord-342785-55r01n0x	87	8	a	a	DT
cord-342785-55r01n0x	87	9	Marburg	Marburg	NNP
cord-342785-55r01n0x	87	10	virus	virus	NN
cord-342785-55r01n0x	87	11	assay	assay	NN
cord-342785-55r01n0x	87	12	[	[	-LRB-
cord-342785-55r01n0x	87	13	9	9	CD
cord-342785-55r01n0x	87	14	]	]	-RRB-
cord-342785-55r01n0x	87	15	were	be	VBD
cord-342785-55r01n0x	87	16	of	of	IN
cord-342785-55r01n0x	87	17	the	the	DT
cord-342785-55r01n0x	87	18	Lake	Lake	NNP
cord-342785-55r01n0x	87	19	Victoria	Victoria	NNP
cord-342785-55r01n0x	87	20	variety	variety	NN
cord-342785-55r01n0x	87	21	.	.	.
cord-342785-55r01n0x	88	1	False	false	JJ
cord-342785-55r01n0x	88	2	negatives	negative	NNS
cord-342785-55r01n0x	88	3	associated	associate	VBN
cord-342785-55r01n0x	88	4	with	with	IN
cord-342785-55r01n0x	88	5	a	a	DT
cord-342785-55r01n0x	88	6	yellow	yellow	JJ
cord-342785-55r01n0x	88	7	fever	fever	NN
cord-342785-55r01n0x	88	8	signature	signature	NN
cord-342785-55r01n0x	88	9	[	[	-LRB-
cord-342785-55r01n0x	88	10	10	10	CD
cord-342785-55r01n0x	88	11	]	]	-RRB-
cord-342785-55r01n0x	88	12	included	include	VBD
cord-342785-55r01n0x	88	13	Trinidad	Trinidad	NNP
cord-342785-55r01n0x	88	14	,	,	,
cord-342785-55r01n0x	88	15	French	french	JJ
cord-342785-55r01n0x	88	16	neurotropic	neurotropic	NNS
cord-342785-55r01n0x	88	17	,	,	,
cord-342785-55r01n0x	88	18	French	french	JJ
cord-342785-55r01n0x	88	19	viscerotropic	viscerotropic	NN
cord-342785-55r01n0x	88	20	,	,	,
cord-342785-55r01n0x	88	21	and	and	CC
cord-342785-55r01n0x	88	22	vaccine	vaccine	NN
cord-342785-55r01n0x	88	23	strains	strain	NNS
cord-342785-55r01n0x	88	24	.	.	.
cord-342785-55r01n0x	89	1	The	the	DT
cord-342785-55r01n0x	89	2	Filoviridae	Filoviridae	NNP
cord-342785-55r01n0x	89	3	(	(	-LRB-
cord-342785-55r01n0x	89	4	Ebola	Ebola	NNP
cord-342785-55r01n0x	89	5	/	/	SYM
cord-342785-55r01n0x	89	6	Marburg	Marburg	NNP
cord-342785-55r01n0x	89	7	)	)	-RRB-
cord-342785-55r01n0x	89	8	assay	assay	NN
cord-342785-55r01n0x	89	9	[	[	-LRB-
cord-342785-55r01n0x	89	10	10	10	CD
cord-342785-55r01n0x	89	11	]	]	-RRB-
cord-342785-55r01n0x	89	12	detected	detect	VBN
cord-342785-55r01n0x	89	13	only	only	RB
cord-342785-55r01n0x	89	14	Ebola	Ebola	NNP
cord-342785-55r01n0x	89	15	viruses	virus	NNS
cord-342785-55r01n0x	89	16	.	.	.
cord-342785-55r01n0x	90	1	Staphylococcus	staphylococcus	NN
cord-342785-55r01n0x	90	2	aureus	aureus	NN
cord-342785-55r01n0x	90	3	[	[	-LRB-
cord-342785-55r01n0x	90	4	11	11	CD
cord-342785-55r01n0x	90	5	]	]	-RRB-
cord-342785-55r01n0x	90	6	and	and	CC
cord-342785-55r01n0x	90	7	Enterobacteriaceae	Enterobacteriaceae	NNP
cord-342785-55r01n0x	90	8	assays	assay	NNS
cord-342785-55r01n0x	90	9	[	[	-LRB-
cord-342785-55r01n0x	90	10	12	12	CD
cord-342785-55r01n0x	90	11	]	]	-RRB-
cord-342785-55r01n0x	90	12	had	have	VBD
cord-342785-55r01n0x	90	13	low	low	JJ
cord-342785-55r01n0x	90	14	sensitivity	sensitivity	NN
cord-342785-55r01n0x	90	15	.	.	.
cord-342785-55r01n0x	91	1	An	an	DT
cord-342785-55r01n0x	91	2	Escherichia	Escherichia	NNP
cord-342785-55r01n0x	91	3	coli	coli	NNS
cord-342785-55r01n0x	91	4	assay	assay	NN
cord-342785-55r01n0x	91	5	[	[	-LRB-
cord-342785-55r01n0x	91	6	12	12	CD
cord-342785-55r01n0x	91	7	]	]	-RRB-
cord-342785-55r01n0x	91	8	hit	hit	VBN
cord-342785-55r01n0x	91	9	Shigella	Shigella	NNP
cord-342785-55r01n0x	91	10	and	and	CC
cord-342785-55r01n0x	91	11	Vibrio	Vibrio	NNP
cord-342785-55r01n0x	91	12	sequences	sequence	NNS
cord-342785-55r01n0x	91	13	.	.	.
cord-342785-55r01n0x	92	1	Many	many	JJ
cord-342785-55r01n0x	92	2	of	of	IN
cord-342785-55r01n0x	92	3	these	these	DT
cord-342785-55r01n0x	92	4	signatures	signature	NNS
cord-342785-55r01n0x	92	5	[	[	-LRB-
cord-342785-55r01n0x	92	6	13	13	CD
cord-342785-55r01n0x	92	7	]	]	-RRB-
cord-342785-55r01n0x	92	8	had	have	VBD
cord-342785-55r01n0x	92	9	high	high	JJ
cord-342785-55r01n0x	92	10	sensitivity	sensitivity	NN
cord-342785-55r01n0x	92	11	.	.	.
cord-342785-55r01n0x	93	1	Combining	combine	VBG
cord-342785-55r01n0x	93	2	several	several	JJ
cord-342785-55r01n0x	93	3	of	of	IN
cord-342785-55r01n0x	93	4	them	-PRON-	PRP
cord-342785-55r01n0x	93	5	into	into	IN
cord-342785-55r01n0x	93	6	a	a	DT
cord-342785-55r01n0x	93	7	multiplex	multiplex	JJ
cord-342785-55r01n0x	93	8	assay	assay	NN
cord-342785-55r01n0x	93	9	would	would	MD
cord-342785-55r01n0x	93	10	probably	probably	RB
cord-342785-55r01n0x	93	11	improve	improve	VB
cord-342785-55r01n0x	93	12	sensitivity	sensitivity	NN
cord-342785-55r01n0x	93	13	further	further	RB
cord-342785-55r01n0x	93	14	.	.	.
cord-342785-55r01n0x	94	1	These	these	DT
cord-342785-55r01n0x	94	2	signatures	signature	NNS
cord-342785-55r01n0x	94	3	were	be	VBD
cord-342785-55r01n0x	94	4	designed	design	VBN
cord-342785-55r01n0x	94	5	using	use	VBG
cord-342785-55r01n0x	94	6	a	a	DT
cord-342785-55r01n0x	94	7	minimal	minimal	JJ
cord-342785-55r01n0x	94	8	set	set	NN
cord-342785-55r01n0x	94	9	clustering	clustering	NN
cord-342785-55r01n0x	94	10	approach	approach	NN
cord-342785-55r01n0x	94	11	[	[	-LRB-
cord-342785-55r01n0x	94	12	14	14	CD
cord-342785-55r01n0x	94	13	]	]	-RRB-
cord-342785-55r01n0x	94	14	.	.	.
cord-342785-55r01n0x	95	1	While	while	IN
cord-342785-55r01n0x	95	2	individual	individual	JJ
cord-342785-55r01n0x	95	3	signatures	signature	NNS
cord-342785-55r01n0x	95	4	have	have	VBP
cord-342785-55r01n0x	95	5	decent	decent	JJ
cord-342785-55r01n0x	95	6	sensitivity	sensitivity	NN
cord-342785-55r01n0x	95	7	,	,	,
cord-342785-55r01n0x	95	8	combining	combine	VBG
cord-342785-55r01n0x	95	9	several	several	JJ
cord-342785-55r01n0x	95	10	signatures	signature	NNS
cord-342785-55r01n0x	95	11	in	in	IN
cord-342785-55r01n0x	95	12	one	one	CD
cord-342785-55r01n0x	95	13	assay	assay	NN
cord-342785-55r01n0x	95	14	,	,	,
cord-342785-55r01n0x	95	15	as	as	IN
cord-342785-55r01n0x	95	16	advocated	advocate	VBN
cord-342785-55r01n0x	95	17	in	in	IN
cord-342785-55r01n0x	95	18	the	the	DT
cord-342785-55r01n0x	95	19	publication	publication	NN
cord-342785-55r01n0x	95	20	greatly	greatly	RB
cord-342785-55r01n0x	95	21	improved	improve	VBD
cord-342785-55r01n0x	95	22	sensitivity	sensitivity	NN
cord-342785-55r01n0x	95	23	.	.	.
cord-342785-55r01n0x	96	1	The	the	DT
cord-342785-55r01n0x	96	2	signatures	signature	NNS
cord-342785-55r01n0x	96	3	for	for	IN
cord-342785-55r01n0x	96	4	Hepatitis	Hepatitis	NNP
cord-342785-55r01n0x	96	5	A	A	NNP
cord-342785-55r01n0x	96	6	are	be	VBP
cord-342785-55r01n0x	96	7	currently	currently	RB
cord-342785-55r01n0x	96	8	undergoing	undergo	VBG
cord-342785-55r01n0x	96	9	laboratory	laboratory	NN
cord-342785-55r01n0x	96	10	screening	screening	NN
cord-342785-55r01n0x	96	11	by	by	IN
cord-342785-55r01n0x	96	12	the	the	DT
cord-342785-55r01n0x	96	13	FDA	FDA	NNP
cord-342785-55r01n0x	96	14	,	,	,
cord-342785-55r01n0x	96	15	and	and	CC
cord-342785-55r01n0x	96	16	are	be	VBP
cord-342785-55r01n0x	96	17	performing	perform	VBG
cord-342785-55r01n0x	96	18	well	well	RB
cord-342785-55r01n0x	96	19	(	(	-LRB-
cord-342785-55r01n0x	96	20	G.	G.	NNP
cord-342785-55r01n0x	96	21	Hartman	Hartman	NNP
cord-342785-55r01n0x	96	22	,	,	,
cord-342785-55r01n0x	96	23	personal	personal	JJ
cord-342785-55r01n0x	96	24	communication	communication	NN
cord-342785-55r01n0x	96	25	)	)	-RRB-
cord-342785-55r01n0x	96	26	.	.	.
cord-342785-55r01n0x	97	1	Several	several	JJ
cord-342785-55r01n0x	97	2	reported	report	VBN
cord-342785-55r01n0x	97	3	signatures	signature	NNS
cord-342785-55r01n0x	97	4	produced	produce	VBD
cord-342785-55r01n0x	97	5	no	no	DT
cord-342785-55r01n0x	97	6	predicted	predict	VBN
cord-342785-55r01n0x	97	7	hits	hit	NNS
cord-342785-55r01n0x	97	8	.	.	.
cord-342785-55r01n0x	98	1	These	these	DT
cord-342785-55r01n0x	98	2	include	include	VBP
cord-342785-55r01n0x	98	3	assays	assay	NNS
cord-342785-55r01n0x	98	4	for	for	IN
cord-342785-55r01n0x	98	5	several	several	JJ
cord-342785-55r01n0x	98	6	flaviviruses	flavivirus	NNS
cord-342785-55r01n0x	98	7	[	[	-LRB-
cord-342785-55r01n0x	98	8	10	10	CD
cord-342785-55r01n0x	98	9	,	,	,
cord-342785-55r01n0x	98	10	15	15	CD
cord-342785-55r01n0x	98	11	,	,	,
cord-342785-55r01n0x	98	12	16	16	CD
cord-342785-55r01n0x	98	13	]	]	-RRB-
cord-342785-55r01n0x	98	14	,	,	,
cord-342785-55r01n0x	98	15	and	and	CC
cord-342785-55r01n0x	98	16	16S	16S	NNP
cord-342785-55r01n0x	98	17	rRNA	rrna	NN
cord-342785-55r01n0x	98	18	assays	assay	NNS
cord-342785-55r01n0x	98	19	[	[	-LRB-
cord-342785-55r01n0x	98	20	12	12	CD
cord-342785-55r01n0x	98	21	]	]	-RRB-
cord-342785-55r01n0x	98	22	for	for	IN
cord-342785-55r01n0x	98	23	several	several	JJ
cord-342785-55r01n0x	98	24	bacteria	bacteria	NNS
cord-342785-55r01n0x	98	25	.	.	.
cord-342785-55r01n0x	99	1	Examination	examination	NN
cord-342785-55r01n0x	99	2	of	of	IN
cord-342785-55r01n0x	99	3	BLAST	blast	NN
cord-342785-55r01n0x	99	4	output	output	NN
cord-342785-55r01n0x	99	5	showed	show	VBD
cord-342785-55r01n0x	99	6	that	that	IN
cord-342785-55r01n0x	99	7	in	in	IN
cord-342785-55r01n0x	99	8	these	these	DT
cord-342785-55r01n0x	99	9	cases	case	NNS
cord-342785-55r01n0x	99	10	either	either	CC
cord-342785-55r01n0x	99	11	a	a	DT
cord-342785-55r01n0x	99	12	primer	primer	NN
cord-342785-55r01n0x	99	13	or	or	CC
cord-342785-55r01n0x	99	14	internal	internal	JJ
cord-342785-55r01n0x	99	15	oligo	oligo	NN
cord-342785-55r01n0x	99	16	(	(	-LRB-
cord-342785-55r01n0x	99	17	probe	probe	NN
cord-342785-55r01n0x	99	18	)	)	-RRB-
cord-342785-55r01n0x	99	19	did	do	VBD
cord-342785-55r01n0x	99	20	not	not	RB
cord-342785-55r01n0x	99	21	have	have	VB
cord-342785-55r01n0x	99	22	BLAST	blast	NN
cord-342785-55r01n0x	99	23	hits	hit	NNS
cord-342785-55r01n0x	99	24	to	to	TO
cord-342785-55r01n0x	99	25	target	target	VB
cord-342785-55r01n0x	99	26	,	,	,
cord-342785-55r01n0x	99	27	there	there	EX
cord-342785-55r01n0x	99	28	were	be	VBD
cord-342785-55r01n0x	99	29	too	too	RB
cord-342785-55r01n0x	99	30	many	many	JJ
cord-342785-55r01n0x	99	31	mismatches	mismatch	NNS
cord-342785-55r01n0x	99	32	per	per	IN
cord-342785-55r01n0x	99	33	primer	primer	NN
cord-342785-55r01n0x	99	34	or	or	CC
cord-342785-55r01n0x	99	35	probe	probe	NN
cord-342785-55r01n0x	99	36	sequence	sequence	NN
cord-342785-55r01n0x	99	37	above	above	IN
cord-342785-55r01n0x	99	38	the	the	DT
cord-342785-55r01n0x	99	39	threshold	threshold	NN
cord-342785-55r01n0x	99	40	specified	specify	VBN
cord-342785-55r01n0x	99	41	in	in	IN
cord-342785-55r01n0x	99	42	our	-PRON-	PRP$
cord-342785-55r01n0x	99	43	anal-	anal-	NN
cord-342785-55r01n0x	100	1	All	all	DT
cord-342785-55r01n0x	101	1	but	but	CC
cord-342785-55r01n0x	101	2	three	three	CD
cord-342785-55r01n0x	101	3	are	be	VBP
cord-342785-55r01n0x	101	4	Taqman	Taqman	NNP
cord-342785-55r01n0x	101	5	signatures	signature	NNS
cord-342785-55r01n0x	101	6	.	.	.
cord-342785-55r01n0x	102	1	The	the	DT
cord-342785-55r01n0x	102	2	three	three	CD
cord-342785-55r01n0x	102	3	intercalating	intercalating	NN
cord-342785-55r01n0x	102	4	dye	dye	NN
cord-342785-55r01n0x	102	5	type	type	NN
cord-342785-55r01n0x	102	6	assays	assay	NNS
cord-342785-55r01n0x	102	7	are	be	VBP
cord-342785-55r01n0x	102	8	bolded	bolde	VBN
cord-342785-55r01n0x	102	9	.	.	.
cord-342785-55r01n0x	103	1	yses	yses	NNP
cord-342785-55r01n0x	103	2	,	,	,
cord-342785-55r01n0x	103	3	or	or	CC
cord-342785-55r01n0x	103	4	there	there	EX
cord-342785-55r01n0x	103	5	were	be	VBD
cord-342785-55r01n0x	103	6	mismatches	mismatch	NNS
cord-342785-55r01n0x	103	7	at	at	IN
cord-342785-55r01n0x	103	8	the	the	DT
cord-342785-55r01n0x	103	9	3	3	CD
cord-342785-55r01n0x	103	10	'	'	NN
cord-342785-55r01n0x	103	11	end	end	NN
cord-342785-55r01n0x	103	12	of	of	IN
cord-342785-55r01n0x	103	13	a	a	DT
cord-342785-55r01n0x	103	14	primer	primer	NN
cord-342785-55r01n0x	103	15	relative	relative	NN
cord-342785-55r01n0x	103	16	to	to	IN
cord-342785-55r01n0x	103	17	target	target	VB
cord-342785-55r01n0x	103	18	.	.	.
cord-342785-55r01n0x	104	1	It	-PRON-	PRP
cord-342785-55r01n0x	104	2	is	be	VBZ
cord-342785-55r01n0x	104	3	possible	possible	JJ
cord-342785-55r01n0x	104	4	that	that	IN
cord-342785-55r01n0x	104	5	if	if	IN
cord-342785-55r01n0x	104	6	the	the	DT
cord-342785-55r01n0x	104	7	sequences	sequence	NNS
cord-342785-55r01n0x	104	8	of	of	IN
cord-342785-55r01n0x	104	9	the	the	DT
cord-342785-55r01n0x	104	10	samples	sample	NNS
cord-342785-55r01n0x	104	11	used	use	VBN
cord-342785-55r01n0x	104	12	in	in	IN
cord-342785-55r01n0x	104	13	the	the	DT
cord-342785-55r01n0x	104	14	laboratory	laboratory	NN
cord-342785-55r01n0x	104	15	differ	differ	VBP
cord-342785-55r01n0x	104	16	from	from	IN
cord-342785-55r01n0x	104	17	available	available	JJ
cord-342785-55r01n0x	104	18	genomic	genomic	JJ
cord-342785-55r01n0x	104	19	data	datum	NNS
cord-342785-55r01n0x	104	20	,	,	,
cord-342785-55r01n0x	104	21	or	or	CC
cord-342785-55r01n0x	104	22	if	if	IN
cord-342785-55r01n0x	104	23	the	the	DT
cord-342785-55r01n0x	104	24	PCR	PCR	NNP
cord-342785-55r01n0x	104	25	reaction	reaction	NN
cord-342785-55r01n0x	104	26	conditions	condition	NNS
cord-342785-55r01n0x	104	27	are	be	VBP
cord-342785-55r01n0x	104	28	performed	perform	VBN
cord-342785-55r01n0x	104	29	at	at	IN
cord-342785-55r01n0x	104	30	low	low	JJ
cord-342785-55r01n0x	104	31	stringency	stringency	NN
cord-342785-55r01n0x	104	32	(	(	-LRB-
cord-342785-55r01n0x	104	33	e.g.	e.g.	IN
cord-342785-55r01n0x	104	34	low	low	JJ
cord-342785-55r01n0x	104	35	annealing	anneal	VBG
cord-342785-55r01n0x	104	36	temperatures	temperature	NNS
cord-342785-55r01n0x	104	37	or	or	CC
cord-342785-55r01n0x	104	38	high	high	JJ
cord-342785-55r01n0x	104	39	salt	salt	NN
cord-342785-55r01n0x	104	40	concentrations	concentration	NNS
cord-342785-55r01n0x	104	41	)	)	-RRB-
cord-342785-55r01n0x	105	1	these	these	DT
cord-342785-55r01n0x	105	2	assays	assay	NNS
cord-342785-55r01n0x	105	3	could	could	MD
cord-342785-55r01n0x	105	4	in	in	IN
cord-342785-55r01n0x	105	5	fact	fact	NN
cord-342785-55r01n0x	105	6	work	work	NN
cord-342785-55r01n0x	105	7	in	in	IN
cord-342785-55r01n0x	105	8	the	the	DT
cord-342785-55r01n0x	105	9	laboratory	laboratory	NN
cord-342785-55r01n0x	105	10	.	.	.
cord-342785-55r01n0x	106	1	However	however	RB
cord-342785-55r01n0x	106	2	,	,	,
cord-342785-55r01n0x	106	3	according	accord	VBG
cord-342785-55r01n0x	106	4	to	to	IN
cord-342785-55r01n0x	106	5	the	the	DT
cord-342785-55r01n0x	106	6	genomic	genomic	JJ
cord-342785-55r01n0x	106	7	data	datum	NNS
cord-342785-55r01n0x	106	8	available	available	JJ
cord-342785-55r01n0x	106	9	,	,	,
cord-342785-55r01n0x	106	10	a	a	DT
cord-342785-55r01n0x	106	11	better	well	JJR
cord-342785-55r01n0x	106	12	match	match	NN
cord-342785-55r01n0x	106	13	of	of	IN
cord-342785-55r01n0x	106	14	primers	primer	NNS
cord-342785-55r01n0x	106	15	and	and	CC
cord-342785-55r01n0x	106	16	probes	probe	NNS
cord-342785-55r01n0x	106	17	to	to	TO
cord-342785-55r01n0x	106	18	target	target	VB
cord-342785-55r01n0x	106	19	is	be	VBZ
cord-342785-55r01n0x	106	20	possible	possible	JJ
cord-342785-55r01n0x	106	21	and	and	CC
cord-342785-55r01n0x	106	22	is	be	VBZ
cord-342785-55r01n0x	106	23	usually	usually	RB
cord-342785-55r01n0x	106	24	desired	desire	VBN
cord-342785-55r01n0x	106	25	for	for	IN
cord-342785-55r01n0x	106	26	high	high	JJ
cord-342785-55r01n0x	106	27	sensitivity	sensitivity	NN
cord-342785-55r01n0x	106	28	detection	detection	NN
cord-342785-55r01n0x	106	29	.	.	.
cord-342785-55r01n0x	107	1	Targeting	target	VBG
cord-342785-55r01n0x	107	2	a	a	DT
cord-342785-55r01n0x	107	3	number	number	NN
cord-342785-55r01n0x	107	4	of	of	IN
cord-342785-55r01n0x	107	5	the	the	DT
cord-342785-55r01n0x	107	6	organisms	organism	NNS
cord-342785-55r01n0x	107	7	for	for	IN
cord-342785-55r01n0x	107	8	which	which	WDT
cord-342785-55r01n0x	107	9	currently	currently	RB
cord-342785-55r01n0x	107	10	published	publish	VBN
cord-342785-55r01n0x	107	11	signatures	signature	NNS
cord-342785-55r01n0x	107	12	were	be	VBD
cord-342785-55r01n0x	107	13	predicted	predict	VBN
cord-342785-55r01n0x	107	14	to	to	TO
cord-342785-55r01n0x	107	15	perform	perform	VB
cord-342785-55r01n0x	107	16	poorly	poorly	RB
cord-342785-55r01n0x	107	17	,	,	,
cord-342785-55r01n0x	107	18	as	as	RB
cord-342785-55r01n0x	107	19	well	well	RB
cord-342785-55r01n0x	107	20	as	as	IN
cord-342785-55r01n0x	107	21	some	some	DT
cord-342785-55r01n0x	107	22	for	for	IN
cord-342785-55r01n0x	107	23	which	which	WDT
cord-342785-55r01n0x	107	24	additional	additional	JJ
cord-342785-55r01n0x	107	25	signatures	signature	NNS
cord-342785-55r01n0x	107	26	may	may	MD
cord-342785-55r01n0x	107	27	be	be	VB
cord-342785-55r01n0x	107	28	desired	desire	VBN
cord-342785-55r01n0x	107	29	(	(	-LRB-
cord-342785-55r01n0x	107	30	even	even	RB
cord-342785-55r01n0x	107	31	though	though	IN
cord-342785-55r01n0x	107	32	published	publish	VBN
cord-342785-55r01n0x	107	33	signatures	signature	NNS
cord-342785-55r01n0x	107	34	may	may	MD
cord-342785-55r01n0x	107	35	perform	perform	VB
cord-342785-55r01n0x	107	36	well	well	RB
cord-342785-55r01n0x	107	37	)	)	-RRB-
cord-342785-55r01n0x	107	38	,	,	,
cord-342785-55r01n0x	107	39	we	-PRON-	PRP
cord-342785-55r01n0x	107	40	generated	generate	VBD
cord-342785-55r01n0x	107	41	new	new	JJ
cord-342785-55r01n0x	107	42	signatures	signature	NNS
cord-342785-55r01n0x	107	43	using	use	VBG
cord-342785-55r01n0x	107	44	Minimal	Minimal	NNP
cord-342785-55r01n0x	107	45	Set	set	NN
cord-342785-55r01n0x	107	46	Clustering	Clustering	NNP
cord-342785-55r01n0x	107	47	(	(	-LRB-
cord-342785-55r01n0x	107	48	MSC	MSC	NNP
cord-342785-55r01n0x	107	49	)	)	-RRB-
cord-342785-55r01n0x	107	50	according	accord	VBG
cord-342785-55r01n0x	107	51	to	to	IN
cord-342785-55r01n0x	107	52	methods	method	NNS
cord-342785-55r01n0x	107	53	previously	previously	RB
cord-342785-55r01n0x	107	54	described	describe	VBD
cord-342785-55r01n0x	107	55	[	[	-LRB-
cord-342785-55r01n0x	107	56	14	14	CD
cord-342785-55r01n0x	107	57	,	,	,
cord-342785-55r01n0x	107	58	17	17	CD
cord-342785-55r01n0x	107	59	]	]	-RRB-
cord-342785-55r01n0x	107	60	.	.	.
cord-342785-55r01n0x	108	1	MSC	MSC	NNP
cord-342785-55r01n0x	108	2	begins	begin	VBZ
cord-342785-55r01n0x	108	3	by	by	IN
cord-342785-55r01n0x	108	4	removing	remove	VBG
cord-342785-55r01n0x	108	5	non	non	JJ
cord-342785-55r01n0x	108	6	-	-	JJ
cord-342785-55r01n0x	108	7	unique	unique	JJ
cord-342785-55r01n0x	108	8	regions	region	NNS
cord-342785-55r01n0x	108	9	from	from	IN
cord-342785-55r01n0x	108	10	consideration	consideration	NN
cord-342785-55r01n0x	108	11	as	as	IN
cord-342785-55r01n0x	108	12	primers	primer	NNS
cord-342785-55r01n0x	108	13	or	or	CC
cord-342785-55r01n0x	108	14	probes	probe	NNS
cord-342785-55r01n0x	108	15	from	from	IN
cord-342785-55r01n0x	108	16	each	each	DT
cord-342785-55r01n0x	108	17	of	of	IN
cord-342785-55r01n0x	108	18	the	the	DT
cord-342785-55r01n0x	108	19	target	target	NN
cord-342785-55r01n0x	108	20	sequences	sequence	NNS
cord-342785-55r01n0x	108	21	relative	relative	JJ
cord-342785-55r01n0x	108	22	to	to	IN
cord-342785-55r01n0x	108	23	a	a	DT
cord-342785-55r01n0x	108	24	database	database	NN
cord-342785-55r01n0x	108	25	of	of	IN
cord-342785-55r01n0x	108	26	nontarget	nontarget	NN
cord-342785-55r01n0x	108	27	bacterial	bacterial	JJ
cord-342785-55r01n0x	108	28	and	and	CC
cord-342785-55r01n0x	108	29	viral	viral	JJ
cord-342785-55r01n0x	108	30	sequences	sequence	NNS
cord-342785-55r01n0x	108	31	.	.	.
cord-342785-55r01n0x	109	1	The	the	DT
cord-342785-55r01n0x	109	2	remaining	remain	VBG
cord-342785-55r01n0x	109	3	unique	unique	JJ
cord-342785-55r01n0x	109	4	regions	region	NNS
cord-342785-55r01n0x	109	5	of	of	IN
cord-342785-55r01n0x	109	6	each	each	DT
cord-342785-55r01n0x	109	7	target	target	NN
cord-342785-55r01n0x	109	8	sequence	sequence	NN
cord-342785-55r01n0x	109	9	are	be	VBP
cord-342785-55r01n0x	109	10	mined	mine	VBN
cord-342785-55r01n0x	109	11	for	for	IN
cord-342785-55r01n0x	109	12	all	all	DT
cord-342785-55r01n0x	109	13	or	or	CC
cord-342785-55r01n0x	109	14	many	many	JJ
cord-342785-55r01n0x	109	15	candidate	candidate	NN
cord-342785-55r01n0x	109	16	signatures	signature	NNS
cord-342785-55r01n0x	109	17	,	,	,
cord-342785-55r01n0x	109	18	without	without	IN
cord-342785-55r01n0x	109	19	regard	regard	NN
cord-342785-55r01n0x	109	20	for	for	IN
cord-342785-55r01n0x	109	21	conservation	conservation	NN
cord-342785-55r01n0x	109	22	among	among	IN
cord-342785-55r01n0x	109	23	other	other	JJ
cord-342785-55r01n0x	109	24	targets	target	NNS
cord-342785-55r01n0x	109	25	,	,	,
cord-342785-55r01n0x	109	26	yet	yet	CC
cord-342785-55r01n0x	109	27	satisfying	satisfy	VBG
cord-342785-55r01n0x	109	28	user	user	NN
cord-342785-55r01n0x	109	29	specifications	specification	NNS
cord-342785-55r01n0x	109	30	for	for	IN
cord-342785-55r01n0x	109	31	primer	primer	NN
cord-342785-55r01n0x	109	32	and	and	CC
cord-342785-55r01n0x	109	33	probe	probe	NN
cord-342785-55r01n0x	109	34	length	length	NN
cord-342785-55r01n0x	109	35	,	,	,
cord-342785-55r01n0x	109	36	T	T	NNP
cord-342785-55r01n0x	109	37	m	m	NN
cord-342785-55r01n0x	109	38	,	,	,
cord-342785-55r01n0x	109	39	GC%	gc%	NN
cord-342785-55r01n0x	109	40	,	,	,
cord-342785-55r01n0x	109	41	avoidance	avoidance	NN
cord-342785-55r01n0x	109	42	of	of	IN
cord-342785-55r01n0x	109	43	long	long	JJ
cord-342785-55r01n0x	109	44	homopolymer	homopolymer	NN
cord-342785-55r01n0x	109	45	runs	run	NNS
cord-342785-55r01n0x	109	46	,	,	,
cord-342785-55r01n0x	109	47	and	and	CC
cord-342785-55r01n0x	109	48	amplicon	amplicon	NN
cord-342785-55r01n0x	109	49	length	length	NN
cord-342785-55r01n0x	109	50	.	.	.
cord-342785-55r01n0x	110	1	All	all	DT
cord-342785-55r01n0x	110	2	candidate	candidate	NN
cord-342785-55r01n0x	110	3	signatures	signature	NNS
cord-342785-55r01n0x	110	4	are	be	VBP
cord-342785-55r01n0x	110	5	compared	compare	VBN
cord-342785-55r01n0x	110	6	to	to	IN
cord-342785-55r01n0x	110	7	all	all	DT
cord-342785-55r01n0x	110	8	targets	target	NNS
cord-342785-55r01n0x	110	9	and	and	CC
cord-342785-55r01n0x	110	10	clustered	cluster	VBN
cord-342785-55r01n0x	110	11	by	by	IN
cord-342785-55r01n0x	110	12	the	the	DT
cord-342785-55r01n0x	110	13	subset	subset	NN
cord-342785-55r01n0x	110	14	of	of	IN
cord-342785-55r01n0x	110	15	targets	target	NNS
cord-342785-55r01n0x	110	16	they	-PRON-	PRP
cord-342785-55r01n0x	110	17	are	be	VBP
cord-342785-55r01n0x	110	18	predicted	predict	VBN
cord-342785-55r01n0x	110	19	to	to	TO
cord-342785-55r01n0x	110	20	detect	detect	VB
cord-342785-55r01n0x	110	21	.	.	.
cord-342785-55r01n0x	111	1	Signatures	signature	NNS
cord-342785-55r01n0x	111	2	within	within	IN
cord-342785-55r01n0x	111	3	a	a	DT
cord-342785-55r01n0x	111	4	given	give	VBN
cord-342785-55r01n0x	111	5	cluster	cluster	NN
cord-342785-55r01n0x	111	6	are	be	VBP
cord-342785-55r01n0x	111	7	equivalent	equivalent	JJ
cord-342785-55r01n0x	111	8	,	,	,
cord-342785-55r01n0x	111	9	in	in	IN
cord-342785-55r01n0x	111	10	that	that	IN
cord-342785-55r01n0x	111	11	they	-PRON-	PRP
cord-342785-55r01n0x	111	12	are	be	VBP
cord-342785-55r01n0x	111	13	predicted	predict	VBN
cord-342785-55r01n0x	111	14	to	to	TO
cord-342785-55r01n0x	111	15	detect	detect	VB
cord-342785-55r01n0x	111	16	the	the	DT
cord-342785-55r01n0x	111	17	same	same	JJ
cord-342785-55r01n0x	111	18	subset	subset	NN
cord-342785-55r01n0x	111	19	of	of	IN
cord-342785-55r01n0x	111	20	targets	target	NNS
cord-342785-55r01n0x	111	21	,	,	,
cord-342785-55r01n0x	111	22	so	so	RB
cord-342785-55r01n0x	111	23	by	by	IN
cord-342785-55r01n0x	111	24	clustering	cluster	VBG
cord-342785-55r01n0x	111	25	we	-PRON-	PRP
cord-342785-55r01n0x	111	26	reduce	reduce	VBP
cord-342785-55r01n0x	111	27	the	the	DT
cord-342785-55r01n0x	111	28	redundancy	redundancy	NN
cord-342785-55r01n0x	111	29	and	and	CC
cord-342785-55r01n0x	111	30	size	size	NN
cord-342785-55r01n0x	111	31	of	of	IN
cord-342785-55r01n0x	111	32	the	the	DT
cord-342785-55r01n0x	111	33	problem	problem	NN
cord-342785-55r01n0x	111	34	to	to	IN
cord-342785-55r01n0x	111	35	finding	find	VBG
cord-342785-55r01n0x	111	36	a	a	DT
cord-342785-55r01n0x	111	37	small	small	JJ
cord-342785-55r01n0x	111	38	set	set	NN
cord-342785-55r01n0x	111	39	of	of	IN
cord-342785-55r01n0x	111	40	signatures	signature	NNS
cord-342785-55r01n0x	111	41	that	that	WDT
cord-342785-55r01n0x	111	42	detect	detect	VBP
cord-342785-55r01n0x	111	43	all	all	DT
cord-342785-55r01n0x	111	44	targets	target	NNS
cord-342785-55r01n0x	111	45	.	.	.
cord-342785-55r01n0x	112	1	Nevertheless	nevertheless	RB
cord-342785-55r01n0x	112	2	,	,	,
cord-342785-55r01n0x	112	3	finding	find	VBG
cord-342785-55r01n0x	112	4	the	the	DT
cord-342785-55r01n0x	112	5	optimal	optimal	JJ
cord-342785-55r01n0x	112	6	solution	solution	NN
cord-342785-55r01n0x	112	7	of	of	IN
cord-342785-55r01n0x	112	8	the	the	DT
cord-342785-55r01n0x	112	9	fewest	few	JJS
cord-342785-55r01n0x	112	10	clusters	cluster	NNS
cord-342785-55r01n0x	112	11	to	to	TO
cord-342785-55r01n0x	112	12	detect	detect	VB
cord-342785-55r01n0x	112	13	all	all	DT
cord-342785-55r01n0x	112	14	targets	target	NNS
cord-342785-55r01n0x	112	15	is	be	VBZ
cord-342785-55r01n0x	112	16	an	an	DT
cord-342785-55r01n0x	112	17	NP	NP	NNP
cord-342785-55r01n0x	112	18	complete	complete	JJ
cord-342785-55r01n0x	112	19	problem	problem	NN
cord-342785-55r01n0x	112	20	,	,	,
cord-342785-55r01n0x	112	21	so	so	RB
cord-342785-55r01n0x	112	22	for	for	IN
cord-342785-55r01n0x	112	23	large	large	JJ
cord-342785-55r01n0x	112	24	data	data	NN
cord-342785-55r01n0x	112	25	sets	set	NNS
cord-342785-55r01n0x	112	26	we	-PRON-	PRP
cord-342785-55r01n0x	112	27	use	use	VBP
cord-342785-55r01n0x	112	28	a	a	DT
cord-342785-55r01n0x	112	29	greedy	greedy	JJ
cord-342785-55r01n0x	112	30	algorithm	algorithm	NN
cord-342785-55r01n0x	112	31	to	to	TO
cord-342785-55r01n0x	112	32	find	find	VB
cord-342785-55r01n0x	112	33	a	a	DT
cord-342785-55r01n0x	112	34	small	small	JJ
cord-342785-55r01n0x	112	35	number	number	NN
cord-342785-55r01n0x	112	36	of	of	IN
cord-342785-55r01n0x	112	37	clusters	cluster	NNS
cord-342785-55r01n0x	112	38	that	that	WDT
cord-342785-55r01n0x	112	39	together	together	RB
cord-342785-55r01n0x	112	40	should	should	MD
cord-342785-55r01n0x	112	41	pick	pick	VB
cord-342785-55r01n0x	112	42	up	up	RP
cord-342785-55r01n0x	112	43	all	all	DT
cord-342785-55r01n0x	112	44	targets	target	NNS
cord-342785-55r01n0x	112	45	.	.	.
cord-342785-55r01n0x	113	1	In	in	IN
cord-342785-55r01n0x	113	2	the	the	DT
cord-342785-55r01n0x	113	3	supplementary	supplementary	JJ
cord-342785-55r01n0x	113	4	table	table	NN
cord-342785-55r01n0x	113	5	,	,	,
cord-342785-55r01n0x	113	6	we	-PRON-	PRP
cord-342785-55r01n0x	113	7	often	often	RB
cord-342785-55r01n0x	113	8	provide	provide	VBP
cord-342785-55r01n0x	113	9	more	more	JJR
cord-342785-55r01n0x	113	10	than	than	IN
cord-342785-55r01n0x	113	11	one	one	CD
cord-342785-55r01n0x	113	12	alternative	alternative	JJ
cord-342785-55r01n0x	113	13	signature	signature	NN
cord-342785-55r01n0x	113	14	to	to	TO
cord-342785-55r01n0x	113	15	detect	detect	VB
cord-342785-55r01n0x	113	16	a	a	DT
cord-342785-55r01n0x	113	17	given	give	VBN
cord-342785-55r01n0x	113	18	equivalence	equivalence	NN
cord-342785-55r01n0x	113	19	group	group	NN
cord-342785-55r01n0x	113	20	of	of	IN
cord-342785-55r01n0x	113	21	genomes	genome	NNS
cord-342785-55r01n0x	113	22	to	to	TO
cord-342785-55r01n0x	113	23	serve	serve	VB
cord-342785-55r01n0x	113	24	as	as	IN
cord-342785-55r01n0x	113	25	a	a	DT
cord-342785-55r01n0x	113	26	backup	backup	NN
cord-342785-55r01n0x	113	27	should	should	MD
cord-342785-55r01n0x	113	28	a	a	DT
cord-342785-55r01n0x	113	29	signature	signature	NN
cord-342785-55r01n0x	113	30	perform	perform	VB
cord-342785-55r01n0x	113	31	poorly	poorly	RB
cord-342785-55r01n0x	113	32	in	in	IN
cord-342785-55r01n0x	113	33	laboratory	laboratory	NN
cord-342785-55r01n0x	113	34	testing	testing	NN
cord-342785-55r01n0x	113	35	.	.	.
cord-342785-55r01n0x	114	1	Some	some	DT
cord-342785-55r01n0x	114	2	of	of	IN
cord-342785-55r01n0x	114	3	the	the	DT
cord-342785-55r01n0x	114	4	signatures	signature	NNS
cord-342785-55r01n0x	114	5	may	may	MD
cord-342785-55r01n0x	114	6	have	have	VB
cord-342785-55r01n0x	114	7	mismatches	mismatch	NNS
cord-342785-55r01n0x	114	8	to	to	IN
cord-342785-55r01n0x	114	9	some	some	DT
cord-342785-55r01n0x	114	10	of	of	IN
cord-342785-55r01n0x	114	11	their	-PRON-	PRP$
cord-342785-55r01n0x	114	12	intended	intend	VBN
cord-342785-55r01n0x	114	13	targets	target	NNS
cord-342785-55r01n0x	114	14	,	,	,
cord-342785-55r01n0x	114	15	although	although	IN
cord-342785-55r01n0x	114	16	these	these	DT
cord-342785-55r01n0x	114	17	mismatches	mismatch	NNS
cord-342785-55r01n0x	114	18	are	be	VBP
cord-342785-55r01n0x	114	19	not	not	RB
cord-342785-55r01n0x	114	20	predicted	predict	VBN
cord-342785-55r01n0x	114	21	to	to	TO
cord-342785-55r01n0x	114	22	reduce	reduce	VB
cord-342785-55r01n0x	114	23	the	the	DT
cord-342785-55r01n0x	114	24	T	T	NNP
cord-342785-55r01n0x	114	25	m	m	NN
cord-342785-55r01n0x	114	26	of	of	IN
cord-342785-55r01n0x	114	27	primer	primer	NN
cord-342785-55r01n0x	114	28	/	/	SYM
cord-342785-55r01n0x	114	29	probe	probe	NN
cord-342785-55r01n0x	114	30	hybridizing	hybridize	VBG
cord-342785-55r01n0x	114	31	to	to	TO
cord-342785-55r01n0x	114	32	target	target	VB
cord-342785-55r01n0x	114	33	below	below	IN
cord-342785-55r01n0x	114	34	typical	typical	JJ
cord-342785-55r01n0x	114	35	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	114	36	reaction	reaction	NN
cord-342785-55r01n0x	114	37	conditions	condition	NNS
cord-342785-55r01n0x	114	38	.	.	.
cord-342785-55r01n0x	115	1	None	none	NN
cord-342785-55r01n0x	115	2	of	of	IN
cord-342785-55r01n0x	115	3	these	these	DT
cord-342785-55r01n0x	115	4	computationally	computationally	RB
cord-342785-55r01n0x	115	5	predicted	predict	VBN
cord-342785-55r01n0x	115	6	signatures	signature	NNS
cord-342785-55r01n0x	115	7	have	have	VBP
cord-342785-55r01n0x	115	8	been	be	VBN
cord-342785-55r01n0x	115	9	screened	screen	VBN
cord-342785-55r01n0x	115	10	in	in	IN
cord-342785-55r01n0x	115	11	the	the	DT
cord-342785-55r01n0x	115	12	laboratory	laboratory	NN
cord-342785-55r01n0x	115	13	,	,	,
cord-342785-55r01n0x	115	14	as	as	IN
cord-342785-55r01n0x	115	15	this	this	DT
cord-342785-55r01n0x	115	16	is	be	VBZ
cord-342785-55r01n0x	115	17	beyond	beyond	IN
cord-342785-55r01n0x	115	18	the	the	DT
cord-342785-55r01n0x	115	19	scope	scope	NN
cord-342785-55r01n0x	115	20	of	of	IN
cord-342785-55r01n0x	115	21	this	this	DT
cord-342785-55r01n0x	115	22	paper	paper	NN
cord-342785-55r01n0x	115	23	.	.	.
cord-342785-55r01n0x	115	24	 	 	_SP
cord-342785-55r01n0x	116	1	Year	year	NN
cord-342785-55r01n0x	116	2	Target	Target	NNP
cord-342785-55r01n0x	116	3	Pseudomonas	Pseudomonas	NNP
cord-342785-55r01n0x	116	4	aeruginosa	aeruginosa	NN
cord-342785-55r01n0x	116	5	,	,	,
cord-342785-55r01n0x	116	6	Escherichia	Escherichia	NNP
cord-342785-55r01n0x	116	7	coli	coli	NNS
cord-342785-55r01n0x	116	8	,	,	,
cord-342785-55r01n0x	116	9	and	and	CC
cord-342785-55r01n0x	116	10	Neisseria	Neisseria	NNP
cord-342785-55r01n0x	116	11	meningitidis	meningitidis	NNP
cord-342785-55r01n0x	116	12	.	.	.
cord-342785-55r01n0x	117	1	As	as	IN
cord-342785-55r01n0x	117	2	expected	expect	VBN
cord-342785-55r01n0x	117	3	we	-PRON-	PRP
cord-342785-55r01n0x	117	4	found	find	VBD
cord-342785-55r01n0x	117	5	that	that	IN
cord-342785-55r01n0x	117	6	false	false	JJ
cord-342785-55r01n0x	117	7	negatives	negative	NNS
cord-342785-55r01n0x	117	8	were	be	VBD
cord-342785-55r01n0x	117	9	much	much	RB
cord-342785-55r01n0x	117	10	more	more	RBR
cord-342785-55r01n0x	117	11	common	common	JJ
cord-342785-55r01n0x	117	12	than	than	IN
cord-342785-55r01n0x	117	13	false	false	JJ
cord-342785-55r01n0x	117	14	positives	positive	NNS
cord-342785-55r01n0x	117	15	.	.	.
cord-342785-55r01n0x	118	1	Though	though	IN
cord-342785-55r01n0x	118	2	signatures	signature	NNS
cord-342785-55r01n0x	118	3	are	be	VBP
cord-342785-55r01n0x	118	4	generally	generally	RB
cord-342785-55r01n0x	118	5	based	base	VBN
cord-342785-55r01n0x	118	6	on	on	IN
cord-342785-55r01n0x	118	7	conserved	conserve	VBN
cord-342785-55r01n0x	118	8	gene	gene	NN
cord-342785-55r01n0x	118	9	regions	region	NNS
cord-342785-55r01n0x	118	10	,	,	,
cord-342785-55r01n0x	118	11	they	-PRON-	PRP
cord-342785-55r01n0x	118	12	often	often	RB
cord-342785-55r01n0x	118	13	fail	fail	VBP
cord-342785-55r01n0x	118	14	to	to	TO
cord-342785-55r01n0x	118	15	take	take	VB
cord-342785-55r01n0x	118	16	into	into	IN
cord-342785-55r01n0x	118	17	account	account	NN
cord-342785-55r01n0x	118	18	all	all	DT
cord-342785-55r01n0x	118	19	of	of	IN
cord-342785-55r01n0x	118	20	the	the	DT
cord-342785-55r01n0x	118	21	variation	variation	NN
cord-342785-55r01n0x	118	22	within	within	IN
cord-342785-55r01n0x	118	23	a	a	DT
cord-342785-55r01n0x	118	24	target	target	NN
cord-342785-55r01n0x	118	25	set	set	NN
cord-342785-55r01n0x	118	26	of	of	IN
cord-342785-55r01n0x	118	27	organisms	organism	NNS
cord-342785-55r01n0x	118	28	.	.	.
cord-342785-55r01n0x	119	1	This	this	DT
cord-342785-55r01n0x	119	2	may	may	MD
cord-342785-55r01n0x	119	3	be	be	VB
cord-342785-55r01n0x	119	4	because	because	IN
cord-342785-55r01n0x	119	5	the	the	DT
cord-342785-55r01n0x	119	6	signatures	signature	NNS
cord-342785-55r01n0x	119	7	were	be	VBD
cord-342785-55r01n0x	119	8	developed	develop	VBN
cord-342785-55r01n0x	119	9	using	use	VBG
cord-342785-55r01n0x	119	10	sequence	sequence	NN
cord-342785-55r01n0x	119	11	data	datum	NNS
cord-342785-55r01n0x	119	12	from	from	IN
cord-342785-55r01n0x	119	13	a	a	DT
cord-342785-55r01n0x	119	14	handful	handful	NN
cord-342785-55r01n0x	119	15	of	of	IN
cord-342785-55r01n0x	119	16	strains	strain	NNS
cord-342785-55r01n0x	119	17	,	,	,
cord-342785-55r01n0x	119	18	rather	rather	RB
cord-342785-55r01n0x	119	19	than	than	IN
cord-342785-55r01n0x	119	20	a	a	DT
cord-342785-55r01n0x	119	21	thorough	thorough	JJ
cord-342785-55r01n0x	119	22	study	study	NN
cord-342785-55r01n0x	119	23	of	of	IN
cord-342785-55r01n0x	119	24	all	all	DT
cord-342785-55r01n0x	119	25	strains	strain	NNS
cord-342785-55r01n0x	119	26	publicly	publicly	RB
cord-342785-55r01n0x	119	27	available	available	JJ
cord-342785-55r01n0x	119	28	.	.	.
cord-342785-55r01n0x	120	1	These	these	DT
cord-342785-55r01n0x	120	2	false	false	JJ
cord-342785-55r01n0x	120	3	negatives	negative	NNS
cord-342785-55r01n0x	120	4	may	may	MD
cord-342785-55r01n0x	120	5	also	also	RB
cord-342785-55r01n0x	120	6	represent	represent	VB
cord-342785-55r01n0x	120	7	sequences	sequence	NNS
cord-342785-55r01n0x	120	8	that	that	WDT
cord-342785-55r01n0x	120	9	have	have	VBP
cord-342785-55r01n0x	120	10	become	become	VBN
cord-342785-55r01n0x	120	11	available	available	JJ
cord-342785-55r01n0x	120	12	since	since	IN
cord-342785-55r01n0x	120	13	the	the	DT
cord-342785-55r01n0x	120	14	publication	publication	NN
cord-342785-55r01n0x	120	15	of	of	IN
cord-342785-55r01n0x	120	16	the	the	DT
cord-342785-55r01n0x	120	17	given	give	VBN
cord-342785-55r01n0x	120	18	signature	signature	NN
cord-342785-55r01n0x	120	19	.	.	.
cord-342785-55r01n0x	121	1	Since	since	IN
cord-342785-55r01n0x	121	2	new	new	JJ
cord-342785-55r01n0x	121	3	sequence	sequence	NN
cord-342785-55r01n0x	121	4	data	data	NN
cord-342785-55r01n0x	121	5	is	be	VBZ
cord-342785-55r01n0x	121	6	made	make	VBN
cord-342785-55r01n0x	121	7	available	available	JJ
cord-342785-55r01n0x	121	8	at	at	IN
cord-342785-55r01n0x	121	9	an	an	DT
cord-342785-55r01n0x	121	10	ever	ever	RB
cord-342785-55r01n0x	121	11	increasing	increase	VBG
cord-342785-55r01n0x	121	12	rate	rate	NN
cord-342785-55r01n0x	121	13	,	,	,
cord-342785-55r01n0x	121	14	there	there	EX
cord-342785-55r01n0x	121	15	is	be	VBZ
cord-342785-55r01n0x	121	16	great	great	JJ
cord-342785-55r01n0x	121	17	benefit	benefit	NN
cord-342785-55r01n0x	121	18	in	in	IN
cord-342785-55r01n0x	121	19	re	re	NN
cord-342785-55r01n0x	121	20	-	-	NN
cord-342785-55r01n0x	121	21	evaluating	evaluate	VBG
cord-342785-55r01n0x	121	22	clinically	clinically	RB
cord-342785-55r01n0x	121	23	used	use	VBN
cord-342785-55r01n0x	121	24	DNA	dna	NN
cord-342785-55r01n0x	121	25	signatures	signature	NNS
cord-342785-55r01n0x	121	26	regularly	regularly	RB
cord-342785-55r01n0x	121	27	.	.	.
cord-342785-55r01n0x	122	1	When	when	WRB
cord-342785-55r01n0x	122	2	new	new	JJ
cord-342785-55r01n0x	122	3	sequence	sequence	NN
cord-342785-55r01n0x	122	4	data	data	NN
cord-342785-55r01n0x	122	5	leads	lead	VBZ
cord-342785-55r01n0x	122	6	to	to	IN
cord-342785-55r01n0x	122	7	false	false	JJ
cord-342785-55r01n0x	122	8	negative	negative	JJ
cord-342785-55r01n0x	122	9	predictions	prediction	NNS
cord-342785-55r01n0x	122	10	for	for	IN
cord-342785-55r01n0x	122	11	a	a	DT
cord-342785-55r01n0x	122	12	signature	signature	NN
cord-342785-55r01n0x	122	13	,	,	,
cord-342785-55r01n0x	122	14	one	one	CD
cord-342785-55r01n0x	122	15	of	of	IN
cord-342785-55r01n0x	122	16	two	two	CD
cord-342785-55r01n0x	122	17	explanations	explanation	NNS
cord-342785-55r01n0x	122	18	can	can	MD
cord-342785-55r01n0x	122	19	be	be	VB
cord-342785-55r01n0x	122	20	given	give	VBN
cord-342785-55r01n0x	122	21	.	.	.
cord-342785-55r01n0x	123	1	The	the	DT
cord-342785-55r01n0x	123	2	new	new	JJ
cord-342785-55r01n0x	123	3	sequences	sequence	NNS
cord-342785-55r01n0x	123	4	either	either	CC
cord-342785-55r01n0x	123	5	represent	represent	VBP
cord-342785-55r01n0x	123	6	recently	recently	RB
cord-342785-55r01n0x	123	7	recognized	recognize	VBN
cord-342785-55r01n0x	123	8	variation	variation	NN
cord-342785-55r01n0x	123	9	that	that	WDT
cord-342785-55r01n0x	123	10	has	have	VBZ
cord-342785-55r01n0x	123	11	been	be	VBN
cord-342785-55r01n0x	123	12	around	around	RB
cord-342785-55r01n0x	123	13	since	since	IN
cord-342785-55r01n0x	123	14	the	the	DT
cord-342785-55r01n0x	123	15	time	time	NN
cord-342785-55r01n0x	123	16	the	the	DT
cord-342785-55r01n0x	123	17	signature	signature	NN
cord-342785-55r01n0x	123	18	was	be	VBD
cord-342785-55r01n0x	123	19	published	publish	VBN
cord-342785-55r01n0x	123	20	,	,	,
cord-342785-55r01n0x	123	21	or	or	CC
cord-342785-55r01n0x	123	22	new	new	JJ
cord-342785-55r01n0x	123	23	variation	variation	NN
cord-342785-55r01n0x	123	24	,	,	,
cord-342785-55r01n0x	123	25	the	the	DT
cord-342785-55r01n0x	123	26	result	result	NN
cord-342785-55r01n0x	123	27	of	of	IN
cord-342785-55r01n0x	123	28	mutation	mutation	NN
cord-342785-55r01n0x	123	29	and	and	CC
cord-342785-55r01n0x	123	30	natural	natural	JJ
cord-342785-55r01n0x	123	31	selection	selection	NN
cord-342785-55r01n0x	123	32	.	.	.
cord-342785-55r01n0x	124	1	In	in	IN
cord-342785-55r01n0x	124	2	either	either	DT
cord-342785-55r01n0x	124	3	case	case	NN
cord-342785-55r01n0x	124	4	,	,	,
cord-342785-55r01n0x	124	5	an	an	DT
cord-342785-55r01n0x	124	6	improved	improved	JJ
cord-342785-55r01n0x	124	7	or	or	CC
cord-342785-55r01n0x	124	8	additional	additional	JJ
cord-342785-55r01n0x	124	9	signature	signature	NN
cord-342785-55r01n0x	124	10	should	should	MD
cord-342785-55r01n0x	124	11	be	be	VB
cord-342785-55r01n0x	124	12	designed	design	VBN
cord-342785-55r01n0x	124	13	.	.	.
cord-342785-55r01n0x	125	1	High	high	JJ
cord-342785-55r01n0x	125	2	false	false	JJ
cord-342785-55r01n0x	125	3	positive	positive	JJ
cord-342785-55r01n0x	125	4	or	or	CC
cord-342785-55r01n0x	125	5	false	false	JJ
cord-342785-55r01n0x	125	6	negative	negative	JJ
cord-342785-55r01n0x	125	7	rates	rate	NNS
cord-342785-55r01n0x	125	8	do	do	VBP
cord-342785-55r01n0x	125	9	not	not	RB
cord-342785-55r01n0x	125	10	necessarily	necessarily	RB
cord-342785-55r01n0x	125	11	indicate	indicate	VB
cord-342785-55r01n0x	125	12	a	a	DT
cord-342785-55r01n0x	125	13	"	"	``
cord-342785-55r01n0x	125	14	bad	bad	JJ
cord-342785-55r01n0x	125	15	"	"	''
cord-342785-55r01n0x	125	16	DNA	dna	NN
cord-342785-55r01n0x	125	17	assay	assay	NN
cord-342785-55r01n0x	125	18	.	.	.
cord-342785-55r01n0x	126	1	The	the	DT
cord-342785-55r01n0x	126	2	quality	quality	NN
cord-342785-55r01n0x	126	3	of	of	IN
cord-342785-55r01n0x	126	4	an	an	DT
cord-342785-55r01n0x	126	5	assay	assay	NN
cord-342785-55r01n0x	126	6	must	must	MD
cord-342785-55r01n0x	126	7	be	be	VB
cord-342785-55r01n0x	126	8	considered	consider	VBN
cord-342785-55r01n0x	126	9	in	in	IN
cord-342785-55r01n0x	126	10	light	light	NN
cord-342785-55r01n0x	126	11	of	of	IN
cord-342785-55r01n0x	126	12	the	the	DT
cord-342785-55r01n0x	126	13	milieu	milieu	NN
cord-342785-55r01n0x	126	14	in	in	IN
cord-342785-55r01n0x	126	15	which	which	WDT
cord-342785-55r01n0x	126	16	the	the	DT
cord-342785-55r01n0x	126	17	testing	testing	NN
cord-342785-55r01n0x	126	18	will	will	MD
cord-342785-55r01n0x	126	19	take	take	VB
cord-342785-55r01n0x	126	20	place	place	NN
cord-342785-55r01n0x	126	21	.	.	.
cord-342785-55r01n0x	127	1	In	in	IN
cord-342785-55r01n0x	127	2	the	the	DT
cord-342785-55r01n0x	127	3	clinical	clinical	JJ
cord-342785-55r01n0x	127	4	laboratory	laboratory	NN
cord-342785-55r01n0x	127	5	,	,	,
cord-342785-55r01n0x	127	6	a	a	DT
cord-342785-55r01n0x	127	7	signature	signature	NN
cord-342785-55r01n0x	127	8	with	with	IN
cord-342785-55r01n0x	127	9	high	high	JJ
cord-342785-55r01n0x	127	10	sensitivity	sensitivity	NN
cord-342785-55r01n0x	127	11	but	but	CC
cord-342785-55r01n0x	127	12	perhaps	perhaps	RB
cord-342785-55r01n0x	127	13	low	low	JJ
cord-342785-55r01n0x	127	14	specificity	specificity	NN
cord-342785-55r01n0x	127	15	may	may	MD
cord-342785-55r01n0x	127	16	be	be	VB
cord-342785-55r01n0x	127	17	preferred	prefer	VBN
cord-342785-55r01n0x	127	18	over	over	IN
cord-342785-55r01n0x	127	19	a	a	DT
cord-342785-55r01n0x	127	20	test	test	NN
cord-342785-55r01n0x	127	21	with	with	IN
cord-342785-55r01n0x	127	22	lower	low	JJR
cord-342785-55r01n0x	127	23	sensitivity	sensitivity	NN
cord-342785-55r01n0x	127	24	in	in	IN
cord-342785-55r01n0x	127	25	cases	case	NNS
cord-342785-55r01n0x	127	26	where	where	WRB
cord-342785-55r01n0x	127	27	the	the	DT
cord-342785-55r01n0x	127	28	putative	putative	JJ
cord-342785-55r01n0x	127	29	pathogen	pathogen	NN
cord-342785-55r01n0x	127	30	requires	require	VBZ
cord-342785-55r01n0x	127	31	immediate	immediate	JJ
cord-342785-55r01n0x	127	32	treatment	treatment	NN
cord-342785-55r01n0x	127	33	or	or	CC
cord-342785-55r01n0x	127	34	may	may	MD
cord-342785-55r01n0x	127	35	spread	spread	VB
cord-342785-55r01n0x	127	36	quickly	quickly	RB
cord-342785-55r01n0x	127	37	.	.	.
cord-342785-55r01n0x	128	1	The	the	DT
cord-342785-55r01n0x	128	2	case	case	NN
cord-342785-55r01n0x	128	3	of	of	IN
cord-342785-55r01n0x	128	4	antibiotic	antibiotic	JJ
cord-342785-55r01n0x	128	5	resistant	resistant	JJ
cord-342785-55r01n0x	128	6	bacteria	bacteria	NNS
cord-342785-55r01n0x	128	7	probably	probably	RB
cord-342785-55r01n0x	128	8	falls	fall	VBZ
cord-342785-55r01n0x	128	9	in	in	IN
cord-342785-55r01n0x	128	10	this	this	DT
cord-342785-55r01n0x	128	11	category	category	NN
cord-342785-55r01n0x	128	12	.	.	.
cord-342785-55r01n0x	129	1	On	on	IN
cord-342785-55r01n0x	129	2	the	the	DT
cord-342785-55r01n0x	129	3	other	other	JJ
cord-342785-55r01n0x	129	4	hand	hand	NN
cord-342785-55r01n0x	129	5	,	,	,
cord-342785-55r01n0x	129	6	the	the	DT
cord-342785-55r01n0x	129	7	nation	nation	NN
cord-342785-55r01n0x	129	8	's	's	POS
cord-342785-55r01n0x	129	9	BASIS	BASIS	NNP
cord-342785-55r01n0x	129	10	and	and	CC
cord-342785-55r01n0x	129	11	BioWatch	BioWatch	NNP
cord-342785-55r01n0x	129	12	programs	program	NNS
cord-342785-55r01n0x	129	13	insists	insist	VBZ
cord-342785-55r01n0x	129	14	on	on	IN
cord-342785-55r01n0x	129	15	zero	zero	CD
cord-342785-55r01n0x	129	16	false	false	JJ
cord-342785-55r01n0x	129	17	positives	positive	NNS
cord-342785-55r01n0x	129	18	,	,	,
cord-342785-55r01n0x	129	19	so	so	IN
cord-342785-55r01n0x	129	20	as	as	IN
cord-342785-55r01n0x	129	21	to	to	TO
cord-342785-55r01n0x	129	22	avoid	avoid	VB
cord-342785-55r01n0x	129	23	public	public	JJ
cord-342785-55r01n0x	129	24	disturbances	disturbance	NNS
cord-342785-55r01n0x	129	25	due	due	JJ
cord-342785-55r01n0x	129	26	to	to	IN
cord-342785-55r01n0x	129	27	false	false	JJ
cord-342785-55r01n0x	129	28	alarms	alarm	NNS
cord-342785-55r01n0x	129	29	,	,	,
cord-342785-55r01n0x	129	30	while	while	IN
cord-342785-55r01n0x	129	31	still	still	RB
cord-342785-55r01n0x	129	32	aiming	aim	VBG
cord-342785-55r01n0x	129	33	for	for	IN
cord-342785-55r01n0x	129	34	zero	zero	CD
cord-342785-55r01n0x	129	35	false	false	JJ
cord-342785-55r01n0x	129	36	negatives	negative	NNS
cord-342785-55r01n0x	129	37	[	[	-LRB-
cord-342785-55r01n0x	129	38	18	18	CD
cord-342785-55r01n0x	129	39	]	]	-RRB-
cord-342785-55r01n0x	129	40	.	.	.
cord-342785-55r01n0x	130	1	One	one	PRP
cord-342785-55r01n0x	130	2	must	must	MD
cord-342785-55r01n0x	130	3	also	also	RB
cord-342785-55r01n0x	130	4	consider	consider	VB
cord-342785-55r01n0x	130	5	the	the	DT
cord-342785-55r01n0x	130	6	type	type	NN
cord-342785-55r01n0x	130	7	of	of	IN
cord-342785-55r01n0x	130	8	false	false	JJ
cord-342785-55r01n0x	130	9	negative	negative	JJ
cord-342785-55r01n0x	130	10	and	and	CC
cord-342785-55r01n0x	130	11	false	false	JJ
cord-342785-55r01n0x	130	12	positive	positive	JJ
cord-342785-55r01n0x	130	13	results	result	NNS
cord-342785-55r01n0x	130	14	to	to	TO
cord-342785-55r01n0x	130	15	determine	determine	VB
cord-342785-55r01n0x	130	16	their	-PRON-	PRP$
cord-342785-55r01n0x	130	17	relevance	relevance	NN
cord-342785-55r01n0x	130	18	.	.	.
cord-342785-55r01n0x	131	1	For	for	IN
cord-342785-55r01n0x	131	2	instance	instance	NN
cord-342785-55r01n0x	131	3	,	,	,
cord-342785-55r01n0x	131	4	in	in	IN
cord-342785-55r01n0x	131	5	this	this	DT
cord-342785-55r01n0x	131	6	article	article	NN
cord-342785-55r01n0x	131	7	an	an	DT
cord-342785-55r01n0x	131	8	assay	assay	NN
cord-342785-55r01n0x	131	9	for	for	IN
cord-342785-55r01n0x	131	10	human	human	JJ
cord-342785-55r01n0x	131	11	corona	corona	NN
cord-342785-55r01n0x	131	12	virus	virus	NN
cord-342785-55r01n0x	131	13	OC43	OC43	NNP
cord-342785-55r01n0x	131	14	[	[	-LRB-
cord-342785-55r01n0x	131	15	6	6	CD
cord-342785-55r01n0x	131	16	]	]	-RRB-
cord-342785-55r01n0x	131	17	What	what	WP
cord-342785-55r01n0x	131	18	about	about	IN
cord-342785-55r01n0x	131	19	such	such	PDT
cord-342785-55r01n0x	131	20	a	a	DT
cord-342785-55r01n0x	131	21	match	match	NN
cord-342785-55r01n0x	131	22	in	in	IN
cord-342785-55r01n0x	131	23	a	a	DT
cord-342785-55r01n0x	131	24	clinical	clinical	JJ
cord-342785-55r01n0x	131	25	lab	lab	NN
cord-342785-55r01n0x	131	26	in	in	IN
cord-342785-55r01n0x	131	27	Africa	Africa	NNP
cord-342785-55r01n0x	131	28	?	?	.
cord-342785-55r01n0x	132	1	On	on	IN
cord-342785-55r01n0x	132	2	the	the	DT
cord-342785-55r01n0x	132	3	other	other	JJ
cord-342785-55r01n0x	132	4	hand	hand	NN
cord-342785-55r01n0x	132	5	,	,	,
cord-342785-55r01n0x	132	6	the	the	DT
cord-342785-55r01n0x	132	7	echovirus	echovirus	NNP
cord-342785-55r01n0x	132	8	sequences	sequence	NNS
cord-342785-55r01n0x	132	9	that	that	WDT
cord-342785-55r01n0x	132	10	the	the	DT
cord-342785-55r01n0x	132	11	coxsackie	coxsackie	NN
cord-342785-55r01n0x	132	12	B4	B4	NNP
cord-342785-55r01n0x	132	13	assay	assay	NN
cord-342785-55r01n0x	132	14	[	[	-LRB-
cord-342785-55r01n0x	132	15	8	8	CD
cord-342785-55r01n0x	132	16	]	]	-RRB-
cord-342785-55r01n0x	132	17	can	can	MD
cord-342785-55r01n0x	132	18	detect	detect	VB
cord-342785-55r01n0x	132	19	could	could	MD
cord-342785-55r01n0x	132	20	produce	produce	VB
cord-342785-55r01n0x	132	21	misleading	misleading	JJ
cord-342785-55r01n0x	132	22	results	result	NNS
cord-342785-55r01n0x	132	23	in	in	IN
cord-342785-55r01n0x	132	24	any	any	DT
cord-342785-55r01n0x	132	25	clinical	clinical	JJ
cord-342785-55r01n0x	132	26	lab	lab	NN
cord-342785-55r01n0x	132	27	.	.	.
cord-342785-55r01n0x	133	1	The	the	DT
cord-342785-55r01n0x	133	2	false	false	JJ
cord-342785-55r01n0x	133	3	negative	negative	JJ
cord-342785-55r01n0x	133	4	and	and	CC
cord-342785-55r01n0x	133	5	false	false	JJ
cord-342785-55r01n0x	133	6	positive	positive	JJ
cord-342785-55r01n0x	133	7	rates	rate	NNS
cord-342785-55r01n0x	133	8	presented	present	VBN
cord-342785-55r01n0x	133	9	in	in	IN
cord-342785-55r01n0x	133	10	this	this	DT
cord-342785-55r01n0x	133	11	study	study	NN
cord-342785-55r01n0x	133	12	may	may	MD
cord-342785-55r01n0x	133	13	vary	vary	VB
cord-342785-55r01n0x	133	14	substantially	substantially	RB
cord-342785-55r01n0x	133	15	from	from	IN
cord-342785-55r01n0x	133	16	those	those	DT
cord-342785-55r01n0x	133	17	seen	see	VBN
cord-342785-55r01n0x	133	18	empirically	empirically	RB
cord-342785-55r01n0x	133	19	.	.	.
cord-342785-55r01n0x	134	1	This	this	DT
cord-342785-55r01n0x	134	2	is	be	VBZ
cord-342785-55r01n0x	134	3	because	because	IN
cord-342785-55r01n0x	134	4	the	the	DT
cord-342785-55r01n0x	134	5	strains	strain	NNS
cord-342785-55r01n0x	134	6	available	available	JJ
cord-342785-55r01n0x	134	7	in	in	IN
cord-342785-55r01n0x	134	8	a	a	DT
cord-342785-55r01n0x	134	9	laboratory	laboratory	NN
cord-342785-55r01n0x	134	10	may	may	MD
cord-342785-55r01n0x	134	11	differ	differ	VB
cord-342785-55r01n0x	134	12	significantly	significantly	RB
cord-342785-55r01n0x	134	13	from	from	IN
cord-342785-55r01n0x	134	14	the	the	DT
cord-342785-55r01n0x	134	15	sequence	sequence	NN
cord-342785-55r01n0x	134	16	data	datum	NNS
cord-342785-55r01n0x	134	17	available	available	JJ
cord-342785-55r01n0x	134	18	,	,	,
cord-342785-55r01n0x	134	19	or	or	CC
cord-342785-55r01n0x	134	20	because	because	IN
cord-342785-55r01n0x	134	21	the	the	DT
cord-342785-55r01n0x	134	22	empirical	empirical	JJ
cord-342785-55r01n0x	134	23	protocol	protocol	NN
cord-342785-55r01n0x	134	24	is	be	VBZ
cord-342785-55r01n0x	134	25	more	more	RBR
cord-342785-55r01n0x	134	26	or	or	CC
cord-342785-55r01n0x	134	27	less	less	RBR
cord-342785-55r01n0x	134	28	stringent	stringent	JJ
cord-342785-55r01n0x	134	29	than	than	IN
cord-342785-55r01n0x	134	30	the	the	DT
cord-342785-55r01n0x	134	31	sequence	sequence	NN
cord-342785-55r01n0x	134	32	-	-	HYPH
cord-342785-55r01n0x	134	33	based	base	VBN
cord-342785-55r01n0x	134	34	requirements	requirement	NNS
cord-342785-55r01n0x	134	35	we	-PRON-	PRP
cord-342785-55r01n0x	134	36	imposed	impose	VBD
cord-342785-55r01n0x	134	37	,	,	,
cord-342785-55r01n0x	134	38	which	which	WDT
cord-342785-55r01n0x	134	39	allowed	allow	VBD
cord-342785-55r01n0x	134	40	no	no	DT
cord-342785-55r01n0x	134	41	more	more	JJR
cord-342785-55r01n0x	134	42	than	than	IN
cord-342785-55r01n0x	134	43	2	2	CD
cord-342785-55r01n0x	134	44	mismatches	mismatch	NNS
cord-342785-55r01n0x	134	45	per	per	IN
cord-342785-55r01n0x	134	46	primer	primer	NN
cord-342785-55r01n0x	134	47	or	or	CC
cord-342785-55r01n0x	134	48	probe	probe	NN
cord-342785-55r01n0x	134	49	for	for	IN
cord-342785-55r01n0x	134	50	detection	detection	NN
cord-342785-55r01n0x	134	51	.	.	.
cord-342785-55r01n0x	135	1	We	-PRON-	PRP
cord-342785-55r01n0x	135	2	believe	believe	VBP
cord-342785-55r01n0x	135	3	that	that	IN
cord-342785-55r01n0x	135	4	as	as	IN
cord-342785-55r01n0x	135	5	more	more	JJR
cord-342785-55r01n0x	135	6	target	target	NN
cord-342785-55r01n0x	135	7	sequences	sequence	NNS
cord-342785-55r01n0x	135	8	become	become	VBP
cord-342785-55r01n0x	135	9	available	available	JJ
cord-342785-55r01n0x	135	10	,	,	,
cord-342785-55r01n0x	135	11	our	-PRON-	PRP$
cord-342785-55r01n0x	135	12	predicted	predict	VBN
cord-342785-55r01n0x	135	13	false	false	JJ
cord-342785-55r01n0x	135	14	negative	negative	JJ
cord-342785-55r01n0x	135	15	rates	rate	NNS
cord-342785-55r01n0x	135	16	will	will	MD
cord-342785-55r01n0x	135	17	tend	tend	VB
cord-342785-55r01n0x	135	18	to	to	TO
cord-342785-55r01n0x	135	19	increase	increase	VB
cord-342785-55r01n0x	135	20	for	for	IN
cord-342785-55r01n0x	135	21	a	a	DT
cord-342785-55r01n0x	135	22	given	give	VBN
cord-342785-55r01n0x	135	23	published	publish	VBN
cord-342785-55r01n0x	135	24	signature	signature	NN
cord-342785-55r01n0x	135	25	both	both	DT
cord-342785-55r01n0x	135	26	as	as	IN
cord-342785-55r01n0x	135	27	a	a	DT
cord-342785-55r01n0x	135	28	result	result	NN
cord-342785-55r01n0x	135	29	of	of	IN
cord-342785-55r01n0x	135	30	better	well	JJR
cord-342785-55r01n0x	135	31	sampling	sampling	NN
cord-342785-55r01n0x	135	32	of	of	IN
cord-342785-55r01n0x	135	33	diversity	diversity	NN
cord-342785-55r01n0x	135	34	and	and	CC
cord-342785-55r01n0x	135	35	as	as	IN
cord-342785-55r01n0x	135	36	a	a	DT
cord-342785-55r01n0x	135	37	result	result	NN
cord-342785-55r01n0x	135	38	of	of	IN
cord-342785-55r01n0x	135	39	failure	failure	NN
cord-342785-55r01n0x	135	40	to	to	TO
cord-342785-55r01n0x	135	41	detect	detect	VB
cord-342785-55r01n0x	135	42	newly	newly	RB
cord-342785-55r01n0x	135	43	evolved	evolve	VBN
cord-342785-55r01n0x	135	44	variants	variant	NNS
cord-342785-55r01n0x	135	45	.	.	.
cord-342785-55r01n0x	136	1	It	-PRON-	PRP
cord-342785-55r01n0x	136	2	has	have	VBZ
cord-342785-55r01n0x	136	3	been	be	VBN
cord-342785-55r01n0x	136	4	estimated	estimate	VBN
cord-342785-55r01n0x	136	5	that	that	IN
cord-342785-55r01n0x	136	6	a	a	DT
cord-342785-55r01n0x	136	7	minimum	minimum	NN
cord-342785-55r01n0x	136	8	of	of	IN
cord-342785-55r01n0x	136	9	3	3	CD
cord-342785-55r01n0x	136	10	-	-	SYM
cord-342785-55r01n0x	136	11	4	4	CD
cord-342785-55r01n0x	136	12	genomes	genome	NNS
cord-342785-55r01n0x	136	13	are	be	VBP
cord-342785-55r01n0x	136	14	needed	need	VBN
cord-342785-55r01n0x	136	15	in	in	IN
cord-342785-55r01n0x	136	16	order	order	NN
cord-342785-55r01n0x	136	17	to	to	TO
cord-342785-55r01n0x	136	18	computationally	computationally	RB
cord-342785-55r01n0x	136	19	design	design	VB
cord-342785-55r01n0x	136	20	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	136	21	PCR	PCR	NNP
cord-342785-55r01n0x	136	22	signatures	signature	NNS
cord-342785-55r01n0x	136	23	likely	likely	JJ
cord-342785-55r01n0x	136	24	to	to	TO
cord-342785-55r01n0x	136	25	detect	detect	VB
cord-342785-55r01n0x	136	26	most	most	JJS
cord-342785-55r01n0x	136	27	strains	strain	NNS
cord-342785-55r01n0x	136	28	,	,	,
cord-342785-55r01n0x	136	29	with	with	IN
cord-342785-55r01n0x	136	30	those	those	DT
cord-342785-55r01n0x	136	31	isolates	isolate	NNS
cord-342785-55r01n0x	136	32	chosen	choose	VBN
cord-342785-55r01n0x	136	33	for	for	IN
cord-342785-55r01n0x	136	34	sequencing	sequencing	NN
cord-342785-55r01n0x	136	35	that	that	WDT
cord-342785-55r01n0x	136	36	have	have	VBP
cord-342785-55r01n0x	136	37	been	be	VBN
cord-342785-55r01n0x	136	38	selected	select	VBN
cord-342785-55r01n0x	136	39	to	to	TO
cord-342785-55r01n0x	136	40	span	span	VB
cord-342785-55r01n0x	136	41	gradients	gradient	NNS
cord-342785-55r01n0x	136	42	of	of	IN
cord-342785-55r01n0x	136	43	geographic	geographic	JJ
cord-342785-55r01n0x	136	44	,	,	,
cord-342785-55r01n0x	136	45	phenotypic	phenotypic	NNS
cord-342785-55r01n0x	136	46	,	,	,
cord-342785-55r01n0x	136	47	and	and	CC
cord-342785-55r01n0x	136	48	temporal	temporal	JJ
cord-342785-55r01n0x	136	49	variation	variation	NN
cord-342785-55r01n0x	136	50	[	[	-LRB-
cord-342785-55r01n0x	136	51	19	19	CD
cord-342785-55r01n0x	136	52	]	]	-RRB-
cord-342785-55r01n0x	136	53	.	.	.
cord-342785-55r01n0x	137	1	Even	even	RB
cord-342785-55r01n0x	137	2	more	more	JJR
cord-342785-55r01n0x	137	3	than	than	IN
cord-342785-55r01n0x	137	4	4	4	CD
cord-342785-55r01n0x	137	5	genomes	genome	NNS
cord-342785-55r01n0x	137	6	are	be	VBP
cord-342785-55r01n0x	137	7	needed	need	VBN
cord-342785-55r01n0x	137	8	for	for	IN
cord-342785-55r01n0x	137	9	particularly	particularly	RB
cord-342785-55r01n0x	137	10	diverse	diverse	JJ
cord-342785-55r01n0x	137	11	organisms	organism	NNS
cord-342785-55r01n0x	137	12	.	.	.
cord-342785-55r01n0x	138	1	Thus	thus	RB
cord-342785-55r01n0x	138	2	,	,	,
cord-342785-55r01n0x	138	3	older	old	JJR
cord-342785-55r01n0x	138	4	signatures	signature	NNS
cord-342785-55r01n0x	138	5	may	may	MD
cord-342785-55r01n0x	138	6	not	not	RB
cord-342785-55r01n0x	138	7	perform	perform	VB
cord-342785-55r01n0x	138	8	as	as	RB
cord-342785-55r01n0x	138	9	well	well	RB
cord-342785-55r01n0x	138	10	as	as	IN
cord-342785-55r01n0x	138	11	newly	newly	RB
cord-342785-55r01n0x	138	12	developed	develop	VBN
cord-342785-55r01n0x	138	13	signatures	signature	NNS
cord-342785-55r01n0x	138	14	from	from	IN
cord-342785-55r01n0x	138	15	the	the	DT
cord-342785-55r01n0x	138	16	most	most	JJS
cord-342785-55r01n0x	138	17	up	up	IN
cord-342785-55r01n0x	138	18	-	-	HYPH
cord-342785-55r01n0x	138	19	to	to	IN
cord-342785-55r01n0x	138	20	-	-	HYPH
cord-342785-55r01n0x	138	21	date	date	NN
cord-342785-55r01n0x	138	22	sequence	sequence	NN
cord-342785-55r01n0x	138	23	data	datum	NNS
cord-342785-55r01n0x	138	24	.	.	.
cord-342785-55r01n0x	139	1	A	a	DT
cord-342785-55r01n0x	139	2	future	future	JJ
cord-342785-55r01n0x	139	3	study	study	NN
cord-342785-55r01n0x	139	4	of	of	IN
cord-342785-55r01n0x	139	5	interest	interest	NN
cord-342785-55r01n0x	139	6	would	would	MD
cord-342785-55r01n0x	139	7	be	be	VB
cord-342785-55r01n0x	139	8	a	a	DT
cord-342785-55r01n0x	139	9	longitudinal	longitudinal	JJ
cord-342785-55r01n0x	139	10	look	look	NN
cord-342785-55r01n0x	139	11	at	at	IN
cord-342785-55r01n0x	139	12	how	how	WRB
cord-342785-55r01n0x	139	13	these	these	DT
cord-342785-55r01n0x	139	14	rates	rate	NNS
cord-342785-55r01n0x	139	15	continue	continue	VBP
cord-342785-55r01n0x	139	16	to	to	TO
cord-342785-55r01n0x	139	17	change	change	VB
cord-342785-55r01n0x	139	18	over	over	IN
cord-342785-55r01n0x	139	19	time	time	NN
cord-342785-55r01n0x	139	20	as	as	IN
cord-342785-55r01n0x	139	21	additional	additional	JJ
cord-342785-55r01n0x	139	22	sequences	sequence	NNS
cord-342785-55r01n0x	139	23	become	become	VBP
cord-342785-55r01n0x	139	24	available	available	JJ
cord-342785-55r01n0x	139	25	.	.	.
cord-342785-55r01n0x	140	1	This	this	DT
cord-342785-55r01n0x	140	2	study	study	NN
cord-342785-55r01n0x	140	3	could	could	MD
cord-342785-55r01n0x	140	4	be	be	VB
cord-342785-55r01n0x	140	5	performed	perform	VBN
cord-342785-55r01n0x	140	6	retrospectively	retrospectively	RB
cord-342785-55r01n0x	140	7	,	,	,
cord-342785-55r01n0x	140	8	since	since	IN
cord-342785-55r01n0x	140	9	sequence	sequence	NN
cord-342785-55r01n0x	140	10	submission	submission	NN
cord-342785-55r01n0x	140	11	dates	date	NNS
cord-342785-55r01n0x	140	12	are	be	VBP
cord-342785-55r01n0x	140	13	easily	easily	RB
cord-342785-55r01n0x	140	14	obtained	obtain	VBN
cord-342785-55r01n0x	140	15	from	from	IN
cord-342785-55r01n0x	140	16	public	public	JJ
cord-342785-55r01n0x	140	17	databases	database	NNS
cord-342785-55r01n0x	140	18	.	.	.
cord-342785-55r01n0x	141	1	We	-PRON-	PRP
cord-342785-55r01n0x	141	2	also	also	RB
cord-342785-55r01n0x	141	3	hypothesized	hypothesize	VBD
cord-342785-55r01n0x	141	4	that	that	IN
cord-342785-55r01n0x	141	5	the	the	DT
cord-342785-55r01n0x	141	6	wider	wide	JJR
cord-342785-55r01n0x	141	7	the	the	DT
cord-342785-55r01n0x	141	8	intended	intended	JJ
cord-342785-55r01n0x	141	9	scope	scope	NN
cord-342785-55r01n0x	141	10	of	of	IN
cord-342785-55r01n0x	141	11	a	a	DT
cord-342785-55r01n0x	141	12	signature	signature	NN
cord-342785-55r01n0x	141	13	,	,	,
cord-342785-55r01n0x	141	14	the	the	DT
cord-342785-55r01n0x	141	15	lower	low	JJR
cord-342785-55r01n0x	141	16	its	-PRON-	PRP$
cord-342785-55r01n0x	141	17	sensitivity	sensitivity	NN
cord-342785-55r01n0x	141	18	would	would	MD
cord-342785-55r01n0x	141	19	tend	tend	VB
cord-342785-55r01n0x	141	20	to	to	TO
cord-342785-55r01n0x	141	21	be	be	VB
cord-342785-55r01n0x	141	22	.	.	.
cord-342785-55r01n0x	142	1	The	the	DT
cord-342785-55r01n0x	142	2	point	point	NN
cord-342785-55r01n0x	142	3	is	be	VBZ
cord-342785-55r01n0x	142	4	illustrated	illustrate	VBN
cord-342785-55r01n0x	142	5	loosely	loosely	RB
cord-342785-55r01n0x	142	6	in	in	IN
cord-342785-55r01n0x	142	7	our	-PRON-	PRP$
cord-342785-55r01n0x	142	8	data	datum	NNS
cord-342785-55r01n0x	142	9	tables	table	NNS
cord-342785-55r01n0x	142	10	.	.	.
cord-342785-55r01n0x	143	1	Twenty	twenty	CD
cord-342785-55r01n0x	143	2	-	-	HYPH
cord-342785-55r01n0x	143	3	six	six	CD
cord-342785-55r01n0x	143	4	of	of	IN
cord-342785-55r01n0x	143	5	the	the	DT
cord-342785-55r01n0x	143	6	28	28	CD
cord-342785-55r01n0x	143	7	signatures	signature	NNS
cord-342785-55r01n0x	143	8	with	with	IN
cord-342785-55r01n0x	143	9	less	less	JJR
cord-342785-55r01n0x	143	10	than	than	IN
cord-342785-55r01n0x	143	11	10	10	CD
cord-342785-55r01n0x	143	12	publicly	publicly	RB
cord-342785-55r01n0x	143	13	available	available	JJ
cord-342785-55r01n0x	143	14	target	target	NN
cord-342785-55r01n0x	143	15	sequences	sequence	NNS
cord-342785-55r01n0x	143	16	had	have	VBD
cord-342785-55r01n0x	143	17	sensitivities	sensitivity	NNS
cord-342785-55r01n0x	143	18	of	of	IN
cord-342785-55r01n0x	143	19	1	1	CD
cord-342785-55r01n0x	143	20	(	(	-LRB-
cord-342785-55r01n0x	143	21	i.e.	i.e.	FW
cord-342785-55r01n0x	143	22	zero	zero	CD
cord-342785-55r01n0x	143	23	false	false	JJ
cord-342785-55r01n0x	143	24	negatives	negative	NNS
cord-342785-55r01n0x	143	25	)	)	-RRB-
cord-342785-55r01n0x	143	26	,	,	,
cord-342785-55r01n0x	143	27	while	while	IN
cord-342785-55r01n0x	143	28	signatures	signature	NNS
cord-342785-55r01n0x	143	29	with	with	IN
cord-342785-55r01n0x	143	30	10	10	CD
cord-342785-55r01n0x	143	31	or	or	CC
cord-342785-55r01n0x	143	32	more	more	JJR
cord-342785-55r01n0x	143	33	targets	target	NNS
cord-342785-55r01n0x	143	34	had	have	VBD
cord-342785-55r01n0x	143	35	an	an	DT
cord-342785-55r01n0x	143	36	average	average	JJ
cord-342785-55r01n0x	143	37	sensitivity	sensitivity	NN
cord-342785-55r01n0x	143	38	of	of	IN
cord-342785-55r01n0x	143	39	0.710116	0.710116	CD
cord-342785-55r01n0x	143	40	.	.	.
cord-342785-55r01n0x	144	1	However	however	RB
cord-342785-55r01n0x	144	2	this	this	DT
cord-342785-55r01n0x	144	3	approach	approach	NN
cord-342785-55r01n0x	144	4	only	only	RB
cord-342785-55r01n0x	144	5	considers	consider	VBZ
cord-342785-55r01n0x	144	6	scope	scope	NN
cord-342785-55r01n0x	144	7	in	in	IN
cord-342785-55r01n0x	144	8	the	the	DT
cord-342785-55r01n0x	144	9	context	context	NN
cord-342785-55r01n0x	144	10	of	of	IN
cord-342785-55r01n0x	144	11	sequence	sequence	NN
cord-342785-55r01n0x	144	12	data	datum	NNS
cord-342785-55r01n0x	144	13	available	available	JJ
cord-342785-55r01n0x	144	14	.	.	.
cord-342785-55r01n0x	145	1	We	-PRON-	PRP
cord-342785-55r01n0x	145	2	tried	try	VBD
cord-342785-55r01n0x	145	3	to	to	TO
cord-342785-55r01n0x	145	4	demonstrate	demonstrate	VB
cord-342785-55r01n0x	145	5	the	the	DT
cord-342785-55r01n0x	145	6	relationship	relationship	NN
cord-342785-55r01n0x	145	7	between	between	IN
cord-342785-55r01n0x	145	8	specificity	specificity	NN
cord-342785-55r01n0x	145	9	and	and	CC
cord-342785-55r01n0x	145	10	scope	scope	NN
cord-342785-55r01n0x	145	11	at	at	IN
cord-342785-55r01n0x	145	12	a	a	DT
cord-342785-55r01n0x	145	13	more	more	RBR
cord-342785-55r01n0x	145	14	fundamental	fundamental	JJ
cord-342785-55r01n0x	145	15	level	level	NN
cord-342785-55r01n0x	145	16	by	by	IN
cord-342785-55r01n0x	145	17	grouping	group	VBG
cord-342785-55r01n0x	145	18	signatures	signature	NNS
cord-342785-55r01n0x	145	19	by	by	IN
cord-342785-55r01n0x	145	20	the	the	DT
cord-342785-55r01n0x	145	21	taxonomic	taxonomic	JJ
cord-342785-55r01n0x	145	22	level	level	NN
cord-342785-55r01n0x	145	23	of	of	IN
cord-342785-55r01n0x	145	24	their	-PRON-	PRP$
cord-342785-55r01n0x	145	25	target	target	NN
cord-342785-55r01n0x	145	26	as	as	IN
cord-342785-55r01n0x	145	27	shown	show	VBN
cord-342785-55r01n0x	145	28	in	in	IN
cord-342785-55r01n0x	145	29	Figure	figure	NN
cord-342785-55r01n0x	145	30	1	1	CD
cord-342785-55r01n0x	145	31	.	.	.
cord-342785-55r01n0x	146	1	However	however	RB
cord-342785-55r01n0x	146	2	the	the	DT
cord-342785-55r01n0x	146	3	results	result	NNS
cord-342785-55r01n0x	146	4	are	be	VBP
cord-342785-55r01n0x	146	5	misleading	mislead	VBG
cord-342785-55r01n0x	146	6	.	.	.
cord-342785-55r01n0x	147	1	In	in	IN
cord-342785-55r01n0x	147	2	virology	virology	NN
cord-342785-55r01n0x	147	3	,	,	,
cord-342785-55r01n0x	147	4	taxonomic	taxonomic	JJ
cord-342785-55r01n0x	147	5	level	level	NN
cord-342785-55r01n0x	147	6	is	be	VBZ
cord-342785-55r01n0x	147	7	not	not	RB
cord-342785-55r01n0x	147	8	a	a	DT
cord-342785-55r01n0x	147	9	good	good	JJ
cord-342785-55r01n0x	147	10	indicator	indicator	NN
cord-342785-55r01n0x	147	11	of	of	IN
cord-342785-55r01n0x	147	12	nucleotide	nucleotide	JJ
cord-342785-55r01n0x	147	13	diversity	diversity	NN
cord-342785-55r01n0x	147	14	.	.	.
cord-342785-55r01n0x	148	1	For	for	IN
cord-342785-55r01n0x	148	2	instance	instance	NN
cord-342785-55r01n0x	148	3	,	,	,
cord-342785-55r01n0x	148	4	there	there	EX
cord-342785-55r01n0x	148	5	is	be	VBZ
cord-342785-55r01n0x	148	6	more	more	JJR
cord-342785-55r01n0x	148	7	diversity	diversity	NN
cord-342785-55r01n0x	148	8	in	in	IN
cord-342785-55r01n0x	148	9	the	the	DT
cord-342785-55r01n0x	148	10	influenza	influenza	NN
cord-342785-55r01n0x	148	11	A	a	DT
cord-342785-55r01n0x	148	12	species	species	NN
cord-342785-55r01n0x	149	1	then	then	RB
cord-342785-55r01n0x	149	2	there	there	EX
cord-342785-55r01n0x	149	3	is	be	VBZ
cord-342785-55r01n0x	149	4	in	in	IN
cord-342785-55r01n0x	149	5	the	the	DT
cord-342785-55r01n0x	149	6	entire	entire	JJ
cord-342785-55r01n0x	149	7	Filoviridae	Filoviridae	NNP
cord-342785-55r01n0x	149	8	family	family	NN
cord-342785-55r01n0x	149	9	,	,	,
cord-342785-55r01n0x	149	10	which	which	WDT
cord-342785-55r01n0x	149	11	consists	consist	VBZ
cord-342785-55r01n0x	149	12	of	of	IN
cord-342785-55r01n0x	149	13	only	only	RB
cord-342785-55r01n0x	149	14	two	two	CD
cord-342785-55r01n0x	149	15	known	know	VBN
cord-342785-55r01n0x	149	16	genera	genus	NNS
cord-342785-55r01n0x	149	17	:	:	:
cord-342785-55r01n0x	149	18	Ebola	Ebola	NNP
cord-342785-55r01n0x	149	19	-	-	HYPH
cord-342785-55r01n0x	149	20	like	like	JJ
cord-342785-55r01n0x	149	21	viruses	virus	NNS
cord-342785-55r01n0x	149	22	and	and	CC
cord-342785-55r01n0x	149	23	Marburg	Marburg	NNP
cord-342785-55r01n0x	149	24	viruses	virus	NNS
cord-342785-55r01n0x	149	25	.	.	.
cord-342785-55r01n0x	150	1	A	a	DT
cord-342785-55r01n0x	150	2	better	well	JJR
cord-342785-55r01n0x	150	3	approach	approach	NN
cord-342785-55r01n0x	150	4	might	may	MD
cord-342785-55r01n0x	150	5	be	be	VB
cord-342785-55r01n0x	150	6	to	to	TO
cord-342785-55r01n0x	150	7	calculate	calculate	VB
cord-342785-55r01n0x	150	8	nucleotide	nucleotide	JJ
cord-342785-55r01n0x	150	9	diversity	diversity	NN
cord-342785-55r01n0x	150	10	as	as	IN
cord-342785-55r01n0x	150	11	a	a	DT
cord-342785-55r01n0x	150	12	function	function	NN
cord-342785-55r01n0x	150	13	of	of	IN
cord-342785-55r01n0x	150	14	phylogenetic	phylogenetic	JJ
cord-342785-55r01n0x	150	15	branch	branch	NN
cord-342785-55r01n0x	150	16	length	length	NN
cord-342785-55r01n0x	150	17	or	or	CC
cord-342785-55r01n0x	150	18	shared	share	VBD
cord-342785-55r01n0x	150	19	k	k	NNP
cord-342785-55r01n0x	150	20	-	-	HYPH
cord-342785-55r01n0x	150	21	mer	mer	NNP
cord-342785-55r01n0x	150	22	clusters	cluster	NNS
cord-342785-55r01n0x	150	23	within	within	IN
cord-342785-55r01n0x	150	24	a	a	DT
cord-342785-55r01n0x	150	25	target	target	NN
cord-342785-55r01n0x	150	26	taxonomy	taxonomy	NN
cord-342785-55r01n0x	150	27	node	node	NN
cord-342785-55r01n0x	150	28	.	.	.
cord-342785-55r01n0x	151	1	Finally	finally	RB
cord-342785-55r01n0x	151	2	,	,	,
cord-342785-55r01n0x	151	3	we	-PRON-	PRP
cord-342785-55r01n0x	151	4	averaged	average	VBD
cord-342785-55r01n0x	151	5	the	the	DT
cord-342785-55r01n0x	151	6	sensitivities	sensitivity	NNS
cord-342785-55r01n0x	151	7	of	of	IN
cord-342785-55r01n0x	151	8	microbes	microbe	NNS
cord-342785-55r01n0x	151	9	by	by	IN
cord-342785-55r01n0x	151	10	genome	genome	NN
cord-342785-55r01n0x	151	11	type	type	NN
cord-342785-55r01n0x	151	12	as	as	IN
cord-342785-55r01n0x	151	13	shown	show	VBN
cord-342785-55r01n0x	151	14	in	in	IN
cord-342785-55r01n0x	151	15	Table	Table	NNP
cord-342785-55r01n0x	151	16	6	6	CD
cord-342785-55r01n0x	151	17	.	.	.
cord-342785-55r01n0x	152	1	Note	note	VB
cord-342785-55r01n0x	152	2	that	that	IN
cord-342785-55r01n0x	152	3	the	the	DT
cord-342785-55r01n0x	152	4	ssRNA	ssRNA	VBG
cord-342785-55r01n0x	152	5	-	-	HYPH
cord-342785-55r01n0x	152	6	RT	RT	NNP
cord-342785-55r01n0x	152	7	category	category	NN
cord-342785-55r01n0x	152	8	includes	include	VBZ
cord-342785-55r01n0x	152	9	only	only	RB
cord-342785-55r01n0x	152	10	HIV-1	HIV-1	NNP
cord-342785-55r01n0x	152	11	.	.	.
cord-342785-55r01n0x	153	1	This	this	DT
cord-342785-55r01n0x	153	2	chart	chart	NN
cord-342785-55r01n0x	153	3	demonstrates	demonstrate	VBZ
cord-342785-55r01n0x	153	4	that	that	IN
cord-342785-55r01n0x	153	5	creating	create	VBG
cord-342785-55r01n0x	153	6	signatures	signature	NNS
cord-342785-55r01n0x	153	7	with	with	IN
cord-342785-55r01n0x	153	8	high	high	JJ
cord-342785-55r01n0x	153	9	sensitivity	sensitivity	NN
cord-342785-55r01n0x	153	10	becomes	become	VBZ
cord-342785-55r01n0x	153	11	more	more	RBR
cord-342785-55r01n0x	153	12	difficult	difficult	JJ
cord-342785-55r01n0x	153	13	for	for	IN
cord-342785-55r01n0x	153	14	target	target	NN
cord-342785-55r01n0x	153	15	organisms	organism	NNS
cord-342785-55r01n0x	153	16	with	with	IN
cord-342785-55r01n0x	153	17	high	high	JJ
cord-342785-55r01n0x	153	18	mutation	mutation	NN
cord-342785-55r01n0x	153	19	rates	rate	NNS
cord-342785-55r01n0x	153	20	.	.	.
cord-342785-55r01n0x	153	21	 	 	_SP
cord-342785-55r01n0x	154	1	Current	current	JJ
cord-342785-55r01n0x	154	2	real	real	JJ
cord-342785-55r01n0x	154	3	-	-	HYPH
cord-342785-55r01n0x	154	4	time	time	NN
cord-342785-55r01n0x	154	5	PCR	PCR	NNP
cord-342785-55r01n0x	154	6	assay	assay	NN
cord-342785-55r01n0x	154	7	design	design	NN
cord-342785-55r01n0x	154	8	approaches	approach	NNS
cord-342785-55r01n0x	154	9	produce	produce	VBP
cord-342785-55r01n0x	154	10	signatures	signature	NNS
cord-342785-55r01n0x	154	11	with	with	IN
cord-342785-55r01n0x	154	12	sensitivities	sensitivity	NNS
cord-342785-55r01n0x	154	13	generally	generally	RB
cord-342785-55r01n0x	154	14	too	too	RB
cord-342785-55r01n0x	154	15	low	low	JJ
cord-342785-55r01n0x	154	16	for	for	IN
cord-342785-55r01n0x	154	17	clinical	clinical	JJ
cord-342785-55r01n0x	154	18	use	use	NN
cord-342785-55r01n0x	154	19	.	.	.
cord-342785-55r01n0x	155	1	We	-PRON-	PRP
cord-342785-55r01n0x	155	2	suggest	suggest	VBP
cord-342785-55r01n0x	155	3	that	that	IN
cord-342785-55r01n0x	155	4	a	a	DT
cord-342785-55r01n0x	155	5	rigorous	rigorous	JJ
cord-342785-55r01n0x	155	6	approach	approach	NN
cord-342785-55r01n0x	155	7	involving	involve	VBG
cord-342785-55r01n0x	155	8	false	false	JJ
cord-342785-55r01n0x	155	9	positive	positive	JJ
cord-342785-55r01n0x	155	10	and	and	CC
cord-342785-55r01n0x	155	11	false	false	JJ
cord-342785-55r01n0x	155	12	negative	negative	JJ
cord-342785-55r01n0x	155	13	analysis	analysis	NN
cord-342785-55r01n0x	155	14	should	should	MD
cord-342785-55r01n0x	155	15	be	be	VB
cord-342785-55r01n0x	155	16	the	the	DT
cord-342785-55r01n0x	155	17	standard	standard	NN
cord-342785-55r01n0x	155	18	by	by	IN
cord-342785-55r01n0x	155	19	which	which	WDT
cord-342785-55r01n0x	155	20	an	an	DT
cord-342785-55r01n0x	155	21	initial	initial	JJ
cord-342785-55r01n0x	155	22	assessment	assessment	NN
cord-342785-55r01n0x	155	23	of	of	IN
cord-342785-55r01n0x	155	24	signature	signature	NN
cord-342785-55r01n0x	155	25	quality	quality	NN
cord-342785-55r01n0x	155	26	is	be	VBZ
cord-342785-55r01n0x	155	27	made	make	VBN
cord-342785-55r01n0x	155	28	.	.	.
cord-342785-55r01n0x	156	1	Signatures	signature	NNS
cord-342785-55r01n0x	156	2	must	must	MD
cord-342785-55r01n0x	156	3	also	also	RB
cord-342785-55r01n0x	156	4	regularly	regularly	RB
cord-342785-55r01n0x	156	5	be	be	VB
cord-342785-55r01n0x	156	6	reassessed	reassess	VBN
cord-342785-55r01n0x	156	7	as	as	IN
cord-342785-55r01n0x	156	8	sequence	sequence	NN
cord-342785-55r01n0x	156	9	data	data	NN
cord-342785-55r01n0x	156	10	becomes	become	VBZ
cord-342785-55r01n0x	156	11	available	available	JJ
cord-342785-55r01n0x	156	12	.	.	.
cord-342785-55r01n0x	157	1	For	for	IN
cord-342785-55r01n0x	157	2	targets	target	NNS
cord-342785-55r01n0x	157	3	with	with	IN
cord-342785-55r01n0x	157	4	wide	wide	JJ
cord-342785-55r01n0x	157	5	nucleotide	nucleotide	JJ
cord-342785-55r01n0x	157	6	diversity	diversity	NN
cord-342785-55r01n0x	157	7	,	,	,
cord-342785-55r01n0x	157	8	it	-PRON-	PRP
cord-342785-55r01n0x	157	9	becomes	become	VBZ
cord-342785-55r01n0x	157	10	necessary	necessary	JJ
cord-342785-55r01n0x	157	11	to	to	TO
cord-342785-55r01n0x	157	12	develop	develop	VB
cord-342785-55r01n0x	157	13	a	a	DT
cord-342785-55r01n0x	157	14	set	set	NN
cord-342785-55r01n0x	157	15	of	of	IN
cord-342785-55r01n0x	157	16	signatures	signature	NNS
cord-342785-55r01n0x	157	17	,	,	,
cord-342785-55r01n0x	157	18	for	for	IN
cord-342785-55r01n0x	157	19	which	which	WDT
cord-342785-55r01n0x	157	20	we	-PRON-	PRP
cord-342785-55r01n0x	157	21	suggest	suggest	VBP
cord-342785-55r01n0x	157	22	a	a	DT
cord-342785-55r01n0x	157	23	minimal	minimal	JJ
cord-342785-55r01n0x	157	24	set	set	NN
cord-342785-55r01n0x	157	25	clustering	clustering	NN
cord-342785-55r01n0x	157	26	approach	approach	NN
cord-342785-55r01n0x	157	27	that	that	WDT
cord-342785-55r01n0x	157	28	may	may	MD
cord-342785-55r01n0x	157	29	also	also	RB
cord-342785-55r01n0x	157	30	include	include	VB
cord-342785-55r01n0x	157	31	signatures	signature	NNS
cord-342785-55r01n0x	157	32	with	with	IN
cord-342785-55r01n0x	157	33	degenerate	degenerate	JJ
cord-342785-55r01n0x	157	34	/	/	SYM
cord-342785-55r01n0x	157	35	inosine	inosine	JJ
cord-342785-55r01n0x	157	36	bases	basis	NNS
cord-342785-55r01n0x	157	37	.	.	.
cord-342785-55r01n0x	158	1	NewRealTimePCRSigatures	newrealtimepcrsigature	NNS
cord-342785-55r01n0x	158	2	.	.	.
cord-342785-55r01n0x	159	1	Fifty	fifty	CD
cord-342785-55r01n0x	159	2	Seven	seven	CD
cord-342785-55r01n0x	159	3	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	160	1	PCR	PCR	NNP
cord-342785-55r01n0x	160	2	primer	primer	NN
cord-342785-55r01n0x	160	3	/	/	SYM
cord-342785-55r01n0x	160	4	probe	probe	NN
cord-342785-55r01n0x	160	5	combinations	combination	NNS
cord-342785-55r01n0x	160	6	we	-PRON-	PRP
cord-342785-55r01n0x	160	7	predict	predict	VBP
cord-342785-55r01n0x	160	8	to	to	TO
cord-342785-55r01n0x	160	9	have	have	VB
cord-342785-55r01n0x	160	10	higher	high	JJR
cord-342785-55r01n0x	160	11	sensitivity	sensitivity	NN
cord-342785-55r01n0x	160	12	/	/	SYM
cord-342785-55r01n0x	160	13	specificity	specificity	NN
cord-342785-55r01n0x	160	14	than	than	IN
cord-342785-55r01n0x	160	15	current	current	JJ
cord-342785-55r01n0x	160	16	published	publish	VBN
cord-342785-55r01n0x	160	17	assays	assay	NNS
cord-342785-55r01n0x	160	18	.	.	.
cord-342785-55r01n0x	161	1	Click	click	VB
cord-342785-55r01n0x	161	2	here	here	RB
cord-342785-55r01n0x	161	3	for	for	IN
cord-342785-55r01n0x	161	4	file	file	NN
cord-342785-55r01n0x	162	1	[	[	-LRB-
cord-342785-55r01n0x	162	2	http://www.biomedcentral.com/content/supplementary/1476-0711-7-18-S1.doc	http://www.biomedcentral.com/content/supplementary/1476-0711-7-18-s1.doc	NN
cord-342785-55r01n0x	162	3	]	]	-RRB-
cord-342785-55r01n0x	162	4	Sensitivity	sensitivity	NN
cord-342785-55r01n0x	162	5	by	by	IN
cord-342785-55r01n0x	162	6	taxonomy	taxonomy	NN
cord-342785-55r01n0x	162	7	level	level	NN
cord-342785-55r01n0x	162	8	Figure	figure	NN
cord-342785-55r01n0x	162	9	1	1	CD
cord-342785-55r01n0x	162	10	Sensitivity	sensitivity	NN
cord-342785-55r01n0x	162	11	by	by	IN
cord-342785-55r01n0x	162	12	taxonomy	taxonomy	NN
cord-342785-55r01n0x	162	13	level	level	NN
cord-342785-55r01n0x	162	14	.	.	.
cord-342785-55r01n0x	163	1	Each	each	DT
cord-342785-55r01n0x	163	2	colored	colored	JJ
cord-342785-55r01n0x	163	3	diamond	diamond	NN
cord-342785-55r01n0x	163	4	represents	represent	VBZ
cord-342785-55r01n0x	163	5	a	a	DT
cord-342785-55r01n0x	163	6	real	real	JJ
cord-342785-55r01n0x	163	7	-	-	HYPH
cord-342785-55r01n0x	163	8	time	time	NN
cord-342785-55r01n0x	163	9	PCR	PCR	NNP
cord-342785-55r01n0x	163	10	assay	assay	NN
cord-342785-55r01n0x	163	11	examined	examine	VBN
cord-342785-55r01n0x	163	12	in	in	IN
cord-342785-55r01n0x	163	13	this	this	DT
cord-342785-55r01n0x	163	14	paper	paper	NN
cord-342785-55r01n0x	163	15	.	.	.
cord-342785-55r01n0x	164	1	Black	black	JJ
cord-342785-55r01n0x	164	2	bars	bar	NNS
cord-342785-55r01n0x	164	3	indicate	indicate	VBP
cord-342785-55r01n0x	164	4	the	the	DT
cord-342785-55r01n0x	164	5	mean	mean	JJ
cord-342785-55r01n0x	164	6	,	,	,
cord-342785-55r01n0x	164	7	grey	grey	NNP
cord-342785-55r01n0x	164	8	bars	bar	NNS
cord-342785-55r01n0x	164	9	indicate	indicate	VBP
cord-342785-55r01n0x	164	10	the	the	DT
cord-342785-55r01n0x	164	11	median	median	NN
cord-342785-55r01n0x	164	12	.	.	.
cord-342785-55r01n0x	165	1	Top	top	JJ
cord-342785-55r01n0x	165	2	and	and	CC
cord-342785-55r01n0x	165	3	bottom	bottom	NN
cord-342785-55r01n0x	165	4	of	of	IN
cord-342785-55r01n0x	165	5	each	each	DT
cord-342785-55r01n0x	165	6	box	box	NN
cord-342785-55r01n0x	165	7	indicates	indicate	VBZ
cord-342785-55r01n0x	165	8	75th	75th	JJ
cord-342785-55r01n0x	165	9	and	and	CC
cord-342785-55r01n0x	165	10	25th	25th	JJ
cord-342785-55r01n0x	165	11	percentiles	percentile	NNS
cord-342785-55r01n0x	165	12	,	,	,
cord-342785-55r01n0x	165	13	and	and	CC
cord-342785-55r01n0x	165	14	grey	grey	JJ
cord-342785-55r01n0x	165	15	lines	line	NNS
cord-342785-55r01n0x	165	16	at	at	IN
cord-342785-55r01n0x	165	17	whisker	whisker	NN
cord-342785-55r01n0x	165	18	ends	end	VBZ
cord-342785-55r01n0x	165	19	denote	denote	VBP
cord-342785-55r01n0x	165	20	min	min	NNP
cord-342785-55r01n0x	165	21	and	and	CC
cord-342785-55r01n0x	165	22	max	max	NNP
cord-342785-55r01n0x	165	23	values	value	NNS
cord-342785-55r01n0x	165	24	.	.	.
cord-342785-55r01n0x	166	1	The	the	DT
cord-342785-55r01n0x	166	2	wide	wide	JJ
cord-342785-55r01n0x	166	3	ranging	range	VBG
cord-342785-55r01n0x	166	4	sensitivities	sensitivity	NNS
cord-342785-55r01n0x	166	5	demonstrate	demonstrate	VBP
cord-342785-55r01n0x	166	6	both	both	DT
cord-342785-55r01n0x	166	7	inconsistency	inconsistency	NN
cord-342785-55r01n0x	166	8	in	in	IN
cord-342785-55r01n0x	166	9	genetic	genetic	JJ
cord-342785-55r01n0x	166	10	diversity	diversity	NN
cord-342785-55r01n0x	166	11	at	at	IN
cord-342785-55r01n0x	166	12	a	a	DT
cord-342785-55r01n0x	166	13	given	give	VBN
cord-342785-55r01n0x	166	14	taxonomy	taxonomy	NN
cord-342785-55r01n0x	166	15	level	level	NN
cord-342785-55r01n0x	166	16	,	,	,
cord-342785-55r01n0x	166	17	and	and	CC
cord-342785-55r01n0x	166	18	inconsistency	inconsistency	NN
cord-342785-55r01n0x	166	19	in	in	IN
cord-342785-55r01n0x	166	20	signature	signature	NN
cord-342785-55r01n0x	166	21	design	design	NN
cord-342785-55r01n0x	166	22	approaches	approach	NNS
cord-342785-55r01n0x	166	23	.	.	.
cord-342785-55r01n0x	167	1	Japanese	japanese	JJ
cord-342785-55r01n0x	167	2	encephalitis	encephalitis	RB
cord-342785-55r01n0x	167	3	virus	virus	NN
cord-342785-55r01n0x	168	1	Too	too	RB
cord-342785-55r01n0x	168	2	many	many	JJ
cord-342785-55r01n0x	168	3	mismatches	mismatch	NNS
cord-342785-55r01n0x	168	4	in	in	IN
cord-342785-55r01n0x	168	5	either	either	CC
cord-342785-55r01n0x	168	6	forward	forward	NN
cord-342785-55r01n0x	168	7	or	or	CC
cord-342785-55r01n0x	168	8	reverse	reverse	VB
cord-342785-55r01n0x	168	9	primer	primer	NN
cord-342785-55r01n0x	168	10	.	.	.
cord-342785-55r01n0x	169	1	Several	several	JJ
cord-342785-55r01n0x	169	2	strains	strain	NNS
cord-342785-55r01n0x	169	3	have	have	VBP
cord-342785-55r01n0x	169	4	3	3	CD
cord-342785-55r01n0x	169	5	mismatches	mismatch	NNS
cord-342785-55r01n0x	169	6	at	at	IN
cord-342785-55r01n0x	169	7	3	3	CD
cord-342785-55r01n0x	169	8	'	'	''
cord-342785-55r01n0x	169	9	end	end	NN
cord-342785-55r01n0x	169	10	of	of	IN
cord-342785-55r01n0x	169	11	forward	forward	JJ
cord-342785-55r01n0x	169	12	primer	primer	NN
cord-342785-55r01n0x	169	13	in	in	IN
cord-342785-55r01n0x	169	14	addition	addition	NN
cord-342785-55r01n0x	169	15	to	to	IN
cord-342785-55r01n0x	169	16	internal	internal	JJ
cord-342785-55r01n0x	169	17	mismatches	mismatch	NNS
cord-342785-55r01n0x	169	18	Reverse	reverse	VB
cord-342785-55r01n0x	169	19	primer	primer	NN
cord-342785-55r01n0x	169	20	only	only	RB
cord-342785-55r01n0x	169	21	has	have	VBZ
cord-342785-55r01n0x	169	22	a	a	DT
cord-342785-55r01n0x	169	23	blast	blast	NN
cord-342785-55r01n0x	169	24	hit	hit	VBN
cord-342785-55r01n0x	169	25	to	to	IN
cord-342785-55r01n0x	169	26	one	one	CD
cord-342785-55r01n0x	169	27	strain	strain	NN
cord-342785-55r01n0x	169	28	(	(	-LRB-
cord-342785-55r01n0x	169	29	Angola71	angola71	NN
cord-342785-55r01n0x	169	30	)	)	-RRB-
cord-342785-55r01n0x	170	1	Saint	Saint	NNP
cord-342785-55r01n0x	170	2	Louis	Louis	NNP
cord-342785-55r01n0x	170	3	encephalitis	encephalitis	RB
cord-342785-55r01n0x	170	4	virus	virus	NN
cord-342785-55r01n0x	170	5	2007	2007	CD
cord-342785-55r01n0x	171	1	Too	too	RB
cord-342785-55r01n0x	171	2	many	many	JJ
cord-342785-55r01n0x	171	3	mismatches	mismatch	NNS
cord-342785-55r01n0x	171	4	in	in	IN
cord-342785-55r01n0x	171	5	the	the	DT
cord-342785-55r01n0x	171	6	reverse	reverse	JJ
cord-342785-55r01n0x	171	7	primer	primer	NN
cord-342785-55r01n0x	171	8	,	,	,
cord-342785-55r01n0x	171	9	with	with	IN
cord-342785-55r01n0x	171	10	3	3	CD
cord-342785-55r01n0x	171	11	mismatches	mismatch	NNS
cord-342785-55r01n0x	171	12	at	at	IN
cord-342785-55r01n0x	171	13	3	3	CD
cord-342785-55r01n0x	171	14	'	'	''
cord-342785-55r01n0x	171	15	end	end	NN
cord-342785-55r01n0x	171	16	as	as	RB
cord-342785-55r01n0x	171	17	well	well	RB
cord-342785-55r01n0x	171	18	as	as	IN
cord-342785-55r01n0x	171	19	at	at	IN
cord-342785-55r01n0x	171	20	other	other	JJ
cord-342785-55r01n0x	171	21	locations	location	NNS
cord-342785-55r01n0x	171	22	Dengue	Dengue	NNP
cord-342785-55r01n0x	171	23	virus	virus	NN
cord-342785-55r01n0x	171	24	1	1	CD
cord-342785-55r01n0x	171	25	2006	2006	CD
cord-342785-55r01n0x	171	26	Reverse	Reverse	NNP
cord-342785-55r01n0x	171	27	primer	primer	NN
cord-342785-55r01n0x	171	28	does	do	VBZ
cord-342785-55r01n0x	171	29	not	not	RB
cord-342785-55r01n0x	171	30	have	have	VB
cord-342785-55r01n0x	171	31	any	any	DT
cord-342785-55r01n0x	171	32	BLAST	blast	NN
cord-342785-55r01n0x	171	33	hits	hit	NNS
cord-342785-55r01n0x	171	34	to	to	TO
cord-342785-55r01n0x	171	35	target	target	VB
cord-342785-55r01n0x	171	36	Dengue	dengue	NN
cord-342785-55r01n0x	171	37	virus	virus	NN
cord-342785-55r01n0x	172	1	2	2	CD
cord-342785-55r01n0x	172	2	2006	2006	CD
cord-342785-55r01n0x	173	1	Forward	forward	JJ
cord-342785-55r01n0x	173	2	primer	primer	NN
cord-342785-55r01n0x	173	3	has	have	VBZ
cord-342785-55r01n0x	173	4	3	3	CD
cord-342785-55r01n0x	173	5	or	or	CC
cord-342785-55r01n0x	173	6	12	12	CD
cord-342785-55r01n0x	173	7	mismatches	mismatch	NNS
cord-342785-55r01n0x	173	8	at	at	IN
cord-342785-55r01n0x	173	9	3	3	CD
cord-342785-55r01n0x	173	10	'	'	''
cord-342785-55r01n0x	173	11	end	end	NN
cord-342785-55r01n0x	173	12	for	for	IN
cord-342785-55r01n0x	173	13	most	most	JJS
cord-342785-55r01n0x	173	14	strains	strain	NNS
cord-342785-55r01n0x	173	15	,	,	,
cord-342785-55r01n0x	173	16	the	the	DT
cord-342785-55r01n0x	173	17	probe	probe	NN
cord-342785-55r01n0x	173	18	has	have	VBZ
cord-342785-55r01n0x	173	19	BLAST	blast	NN
cord-342785-55r01n0x	173	20	hits	hit	NNS
cord-342785-55r01n0x	173	21	to	to	IN
cord-342785-55r01n0x	173	22	only	only	RB
cord-342785-55r01n0x	173	23	7	7	CD
cord-342785-55r01n0x	173	24	of	of	IN
cord-342785-55r01n0x	173	25	the	the	DT
cord-342785-55r01n0x	173	26	57	57	CD
cord-342785-55r01n0x	173	27	genomes	genome	NNS
cord-342785-55r01n0x	173	28	available	available	JJ
cord-342785-55r01n0x	173	29	,	,	,
cord-342785-55r01n0x	173	30	and	and	CC
cord-342785-55r01n0x	173	31	reverse	reverse	VB
cord-342785-55r01n0x	173	32	primer	primer	NN
cord-342785-55r01n0x	173	33	only	only	RB
cord-342785-55r01n0x	173	34	has	have	VBZ
cord-342785-55r01n0x	173	35	a	a	DT
cord-342785-55r01n0x	173	36	BLAST	blast	NN
cord-342785-55r01n0x	173	37	hit	hit	VBN
cord-342785-55r01n0x	173	38	to	to	IN
cord-342785-55r01n0x	173	39	1	1	CD
cord-342785-55r01n0x	173	40	genome	genome	NN
cord-342785-55r01n0x	173	41	Dengue	dengue	NN
cord-342785-55r01n0x	173	42	virus	virus	NN
cord-342785-55r01n0x	173	43	4	4	CD
cord-342785-55r01n0x	173	44	2006	2006	CD
cord-342785-55r01n0x	174	1	Too	too	RB
cord-342785-55r01n0x	174	2	many	many	JJ
cord-342785-55r01n0x	174	3	mismatches	mismatch	NNS
cord-342785-55r01n0x	174	4	in	in	IN
cord-342785-55r01n0x	174	5	forward	forward	JJ
cord-342785-55r01n0x	174	6	primer	primer	NN
cord-342785-55r01n0x	174	7	and	and	CC
cord-342785-55r01n0x	174	8	in	in	IN
cord-342785-55r01n0x	174	9	some	some	DT
cord-342785-55r01n0x	174	10	cases	case	NNS
cord-342785-55r01n0x	174	11	the	the	DT
cord-342785-55r01n0x	174	12	probe	probe	NN
cord-342785-55r01n0x	174	13	Too	too	IN
cord-342785-55r01n0x	174	14	many	many	JJ
cord-342785-55r01n0x	174	15	mismatches	mismatch	NNS
cord-342785-55r01n0x	174	16	in	in	IN
cord-342785-55r01n0x	174	17	forward	forward	JJ
cord-342785-55r01n0x	174	18	primer	primer	NN
cord-342785-55r01n0x	174	19	.	.	.
cord-342785-55r01n0x	175	1	However	however	RB
cord-342785-55r01n0x	175	2	,	,	,
cord-342785-55r01n0x	175	3	they	-PRON-	PRP
cord-342785-55r01n0x	175	4	are	be	VBP
cord-342785-55r01n0x	175	5	at	at	IN
cord-342785-55r01n0x	175	6	the	the	DT
cord-342785-55r01n0x	175	7	5	5	CD
cord-342785-55r01n0x	175	8	'	'	NN
cord-342785-55r01n0x	175	9	end	end	NN
cord-342785-55r01n0x	175	10	,	,	,
cord-342785-55r01n0x	175	11	so	so	RB
cord-342785-55r01n0x	175	12	assay	assay	NN
cord-342785-55r01n0x	175	13	could	could	MD
cord-342785-55r01n0x	175	14	still	still	RB
cord-342785-55r01n0x	175	15	work	work	VB
cord-342785-55r01n0x	175	16	for	for	IN
cord-342785-55r01n0x	175	17	some	some	DT
cord-342785-55r01n0x	175	18	strains	strain	NNS
cord-342785-55r01n0x	175	19	Pseudomonas	Pseudomonas	NNP
cord-342785-55r01n0x	175	20	aeruginosa	aeruginosa	VBZ
cord-342785-55r01n0x	175	21	2004	2004	CD
cord-342785-55r01n0x	176	1	No	no	DT
cord-342785-55r01n0x	176	2	blast	blast	NN
cord-342785-55r01n0x	176	3	hits	hit	NNS
cord-342785-55r01n0x	176	4	of	of	IN
cord-342785-55r01n0x	176	5	probe	probe	NN
cord-342785-55r01n0x	176	6	to	to	IN
cord-342785-55r01n0x	176	7	Pseudomonas	Pseudomonas	NNP
cord-342785-55r01n0x	176	8	aeruginosa	aeruginosa	NN
cord-342785-55r01n0x	176	9	Probe	Probe	NNP
cord-342785-55r01n0x	176	10	is	be	VBZ
cord-342785-55r01n0x	176	11	not	not	RB
cord-342785-55r01n0x	176	12	between	between	IN
cord-342785-55r01n0x	176	13	or	or	CC
cord-342785-55r01n0x	176	14	even	even	RB
cord-342785-55r01n0x	176	15	in	in	IN
cord-342785-55r01n0x	176	16	close	close	JJ
cord-342785-55r01n0x	176	17	proximity	proximity	NN
cord-342785-55r01n0x	176	18	to	to	IN
cord-342785-55r01n0x	176	19	the	the	DT
cord-342785-55r01n0x	176	20	forward	forward	NN
cord-342785-55r01n0x	176	21	and	and	CC
cord-342785-55r01n0x	176	22	reverse	reverse	VB
cord-342785-55r01n0x	176	23	primers	primer	NNS
cord-342785-55r01n0x	176	24	Stenotrophomonas	Stenotrophomonas	NNP
cord-342785-55r01n0x	176	25	maltophilia	maltophilia	NNP
cord-342785-55r01n0x	176	26	2004	2004	CD
cord-342785-55r01n0x	177	1	No	no	DT
cord-342785-55r01n0x	177	2	blast	blast	NN
cord-342785-55r01n0x	177	3	hits	hit	NNS
cord-342785-55r01n0x	177	4	of	of	IN
cord-342785-55r01n0x	177	5	probe	probe	NN
cord-342785-55r01n0x	177	6	to	to	IN
cord-342785-55r01n0x	177	7	Stenotrophomonas	Stenotrophomonas	NNP
cord-342785-55r01n0x	177	8	maltophilia	maltophilia	NNP
cord-342785-55r01n0x	177	9	Probe	Probe	NNP
cord-342785-55r01n0x	177	10	only	only	RB
cord-342785-55r01n0x	177	11	matches	match	VBZ
cord-342785-55r01n0x	177	12	in	in	IN
cord-342785-55r01n0x	177	13	17	17	CD
cord-342785-55r01n0x	177	14	of	of	IN
cord-342785-55r01n0x	177	15	22	22	CD
cord-342785-55r01n0x	177	16	bases	basis	NNS
cord-342785-55r01n0x	177	17	,	,	,
cord-342785-55r01n0x	177	18	which	which	WDT
cord-342785-55r01n0x	177	19	is	be	VBZ
cord-342785-55r01n0x	177	20	unlikely	unlikely	JJ
cord-342785-55r01n0x	177	21	to	to	TO
cord-342785-55r01n0x	177	22	give	give	VB
cord-342785-55r01n0x	177	23	a	a	DT
cord-342785-55r01n0x	177	24	strong	strong	JJ
cord-342785-55r01n0x	177	25	signal	signal	NN
cord-342785-55r01n0x	177	26	,	,	,
cord-342785-55r01n0x	177	27	since	since	IN
cord-342785-55r01n0x	177	28	probe	probe	NN
cord-342785-55r01n0x	177	29	is	be	VBZ
cord-342785-55r01n0x	177	30	unlikely	unlikely	JJ
cord-342785-55r01n0x	177	31	to	to	TO
cord-342785-55r01n0x	177	32	bind	bind	VB
cord-342785-55r01n0x	177	33	prior	prior	RB
cord-342785-55r01n0x	177	34	to	to	IN
cord-342785-55r01n0x	177	35	the	the	DT
cord-342785-55r01n0x	177	36	primers	primer	NNS
cord-342785-55r01n0x	177	37	as	as	IN
cord-342785-55r01n0x	177	38	desired	desire	VBN
cord-342785-55r01n0x	177	39	for	for	IN
cord-342785-55r01n0x	177	40	real	real	JJ
cord-342785-55r01n0x	177	41	time	time	NN
cord-342785-55r01n0x	177	42	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	177	43	chemistry	chemistry	NN
cord-342785-55r01n0x	177	44	.	.	.
cord-342785-55r01n0x	178	1	References	reference	NNS
cord-342785-55r01n0x	178	2	1	1	CD
cord-342785-55r01n0x	179	1	Basic	basic	JJ
cord-342785-55r01n0x	179	2	local	local	JJ
cord-342785-55r01n0x	179	3	alignment	alignment	NN
cord-342785-55r01n0x	179	4	search	search	NN
cord-342785-55r01n0x	179	5	tool	tool	NN
cord-342785-55r01n0x	179	6	Comprehensive	Comprehensive	NNP
cord-342785-55r01n0x	179	7	DNA	dna	NN
cord-342785-55r01n0x	179	8	signature	signature	NN
cord-342785-55r01n0x	179	9	discovery	discovery	NN
cord-342785-55r01n0x	179	10	and	and	CC
cord-342785-55r01n0x	179	11	validation	validation	NN
cord-342785-55r01n0x	180	1	The	the	DT
cord-342785-55r01n0x	180	2	Perl	Perl	NNP
cord-342785-55r01n0x	180	3	Directory	Directory	NNP
cord-342785-55r01n0x	180	4	Frequent	frequent	JJ
cord-342785-55r01n0x	180	5	Detection	Detection	NNP
cord-342785-55r01n0x	180	6	of	of	IN
cord-342785-55r01n0x	180	7	Human	Human	NNP
cord-342785-55r01n0x	180	8	Coronaviruses	Coronaviruses	NNPS
cord-342785-55r01n0x	180	9	in	in	IN
cord-342785-55r01n0x	180	10	Clinical	Clinical	NNP
cord-342785-55r01n0x	180	11	Specimens	specimen	NNS
cord-342785-55r01n0x	180	12	from	from	IN
cord-342785-55r01n0x	180	13	Patients	patient	NNS
cord-342785-55r01n0x	180	14	with	with	IN
cord-342785-55r01n0x	180	15	Respiratory	respiratory	JJ
cord-342785-55r01n0x	180	16	Tract	tract	NN
cord-342785-55r01n0x	180	17	Infection	infection	NN
cord-342785-55r01n0x	180	18	by	by	IN
cord-342785-55r01n0x	180	19	Use	Use	NNP
cord-342785-55r01n0x	180	20	of	of	IN
cord-342785-55r01n0x	180	21	a	a	DT
cord-342785-55r01n0x	180	22	Novel	Novel	NNP
cord-342785-55r01n0x	180	23	Real	Real	NNP
cord-342785-55r01n0x	180	24	-	-	HYPH
cord-342785-55r01n0x	180	25	Time	time	NN
cord-342785-55r01n0x	180	26	Reverse	reverse	NN
cord-342785-55r01n0x	180	27	-	-	HYPH
cord-342785-55r01n0x	180	28	Transcriptase	transcriptase	NN
cord-342785-55r01n0x	180	29	Polymerase	Polymerase	NNP
cord-342785-55r01n0x	180	30	Chain	Chain	NNP
cord-342785-55r01n0x	180	31	Reaction	reaction	NN
cord-342785-55r01n0x	181	1	The	the	DT
cord-342785-55r01n0x	181	2	Interferon	Interferon	NNP
cord-342785-55r01n0x	181	3	Inducer	Inducer	NNP
cord-342785-55r01n0x	181	4	Ampligen	Ampligen	NNP
cord-342785-55r01n0x	181	5	Markedly	markedly	RB
cord-342785-55r01n0x	181	6	Protects	protect	VBZ
cord-342785-55r01n0x	181	7	Mice	mouse	NNS
cord-342785-55r01n0x	181	8	against	against	IN
cord-342785-55r01n0x	181	9	Coxsackie	Coxsackie	NNP
cord-342785-55r01n0x	181	10	B3	B3	NNP
cord-342785-55r01n0x	182	1	Virus	virus	NN
cord-342785-55r01n0x	182	2	-	-	HYPH
cord-342785-55r01n0x	182	3	Induced	induce	VBN
cord-342785-55r01n0x	182	4	Myocarditis	myocarditis	NN
cord-342785-55r01n0x	182	5	Coxsackievirus	Coxsackievirus	NNP
cord-342785-55r01n0x	182	6	B4	B4	NNP
cord-342785-55r01n0x	182	7	Infection	infection	NN
cord-342785-55r01n0x	182	8	of	of	IN
cord-342785-55r01n0x	182	9	Human	Human	NNP
cord-342785-55r01n0x	182	10	Fetal	Fetal	NNP
cord-342785-55r01n0x	182	11	Thymus	thymus	NN
cord-342785-55r01n0x	182	12	Cells	cell	NNS
cord-342785-55r01n0x	182	13	Rapid	rapid	JJ
cord-342785-55r01n0x	182	14	detection	detection	NN
cord-342785-55r01n0x	182	15	protocol	protocol	NN
cord-342785-55r01n0x	182	16	for	for	IN
cord-342785-55r01n0x	182	17	filoviruses	filovirus	NNS
cord-342785-55r01n0x	182	18	Rapid	Rapid	NNP
cord-342785-55r01n0x	182	19	Detection	Detection	NNP
cord-342785-55r01n0x	182	20	and	and	CC
cord-342785-55r01n0x	182	21	Quantification	Quantification	NNP
cord-342785-55r01n0x	182	22	of	of	IN
cord-342785-55r01n0x	182	23	RNA	RNA	NNP
cord-342785-55r01n0x	182	24	of	of	IN
cord-342785-55r01n0x	182	25	Ebola	Ebola	NNP
cord-342785-55r01n0x	182	26	and	and	CC
cord-342785-55r01n0x	182	27	Marburg	Marburg	NNP
cord-342785-55r01n0x	182	28	Viruses	Viruses	NNPS
cord-342785-55r01n0x	182	29	,	,	,
cord-342785-55r01n0x	182	30	Lassa	Lassa	NNP
cord-342785-55r01n0x	182	31	Virus	Virus	NNP
cord-342785-55r01n0x	182	32	,	,	,
cord-342785-55r01n0x	182	33	Crimean	Crimean	NNP
cord-342785-55r01n0x	182	34	-	-	HYPH
cord-342785-55r01n0x	182	35	Congo	Congo	NNP
cord-342785-55r01n0x	182	36	Hemorrhagic	Hemorrhagic	NNP
cord-342785-55r01n0x	182	37	Fever	Fever	NNP
cord-342785-55r01n0x	182	38	Virus	virus	NN
cord-342785-55r01n0x	182	39	,	,	,
cord-342785-55r01n0x	182	40	Rift	Rift	NNP
cord-342785-55r01n0x	182	41	Valley	Valley	NNP
cord-342785-55r01n0x	182	42	Fever	Fever	NNP
cord-342785-55r01n0x	182	43	Virus	Virus	NNP
cord-342785-55r01n0x	182	44	,	,	,
cord-342785-55r01n0x	182	45	Dengue	Dengue	NNP
cord-342785-55r01n0x	182	46	Virus	Virus	NNP
cord-342785-55r01n0x	182	47	,	,	,
cord-342785-55r01n0x	182	48	and	and	CC
cord-342785-55r01n0x	182	49	Yellow	Yellow	NNP
cord-342785-55r01n0x	182	50	Fever	Fever	NNP
cord-342785-55r01n0x	182	51	Virus	virus	NN
cord-342785-55r01n0x	182	52	by	by	IN
cord-342785-55r01n0x	182	53	Real	Real	NNP
cord-342785-55r01n0x	182	54	-	-	HYPH
cord-342785-55r01n0x	182	55	Time	Time	NNP
cord-342785-55r01n0x	182	56	Reverse	Reverse	NNP
cord-342785-55r01n0x	182	57	Transcription	Transcription	NNP
cord-342785-55r01n0x	182	58	-	-	HYPH
cord-342785-55r01n0x	182	59	PCR	PCR	NNP
cord-342785-55r01n0x	183	1	A	a	DT
cord-342785-55r01n0x	183	2	5	5	CD
cord-342785-55r01n0x	184	1	'	'	``
cord-342785-55r01n0x	184	2	Nuclease	Nuclease	NNP
cord-342785-55r01n0x	184	3	PCR	PCR	NNP
cord-342785-55r01n0x	184	4	(	(	-LRB-
cord-342785-55r01n0x	184	5	Taq	Taq	NNP
cord-342785-55r01n0x	184	6	-	-	HYPH
cord-342785-55r01n0x	184	7	Man	Man	NNP
cord-342785-55r01n0x	184	8	)	)	-RRB-
cord-342785-55r01n0x	184	9	High	High	NNP
cord-342785-55r01n0x	184	10	-	-	HYPH
cord-342785-55r01n0x	184	11	Throughput	Throughput	NNP
cord-342785-55r01n0x	184	12	Assay	Assay	NNP
cord-342785-55r01n0x	184	13	for	for	IN
cord-342785-55r01n0x	184	14	Detection	detection	NN
cord-342785-55r01n0x	184	15	of	of	IN
cord-342785-55r01n0x	184	16	the	the	DT
cord-342785-55r01n0x	184	17	mecA	mecA	NNP
cord-342785-55r01n0x	184	18	Gene	Gene	NNP
cord-342785-55r01n0x	184	19	in	in	IN
cord-342785-55r01n0x	184	20	Staphylococci	Staphylococci	NNP
cord-342785-55r01n0x	184	21	Algorithm	Algorithm	NNP
cord-342785-55r01n0x	184	22	for	for	IN
cord-342785-55r01n0x	184	23	the	the	DT
cord-342785-55r01n0x	184	24	identification	identification	NN
cord-342785-55r01n0x	184	25	of	of	IN
cord-342785-55r01n0x	184	26	bacterial	bacterial	JJ
cord-342785-55r01n0x	184	27	pathogens	pathogen	NNS
cord-342785-55r01n0x	184	28	in	in	IN
cord-342785-55r01n0x	184	29	positive	positive	JJ
cord-342785-55r01n0x	184	30	blood	blood	NN
cord-342785-55r01n0x	184	31	cultures	culture	NNS
cord-342785-55r01n0x	184	32	by	by	IN
cord-342785-55r01n0x	184	33	real	real	JJ
cord-342785-55r01n0x	184	34	-	-	HYPH
cord-342785-55r01n0x	184	35	time	time	NN
cord-342785-55r01n0x	184	36	LightCycler	lightcycler	NN
cord-342785-55r01n0x	184	37	polymerase	polymerase	NN
cord-342785-55r01n0x	184	38	chain	chain	NN
cord-342785-55r01n0x	184	39	reaction	reaction	NN
cord-342785-55r01n0x	184	40	(	(	-LRB-
cord-342785-55r01n0x	184	41	PCR	PCR	NNP
cord-342785-55r01n0x	184	42	)	)	-RRB-
cord-342785-55r01n0x	184	43	with	with	IN
cord-342785-55r01n0x	184	44	sequence	sequence	NN
cord-342785-55r01n0x	184	45	-	-	HYPH
cord-342785-55r01n0x	184	46	specific	specific	JJ
cord-342785-55r01n0x	184	47	probes	probe	NNS
cord-342785-55r01n0x	184	48	.	.	.
cord-342785-55r01n0x	185	1	Diagnostic	Diagnostic	NNP
cord-342785-55r01n0x	185	2	Microbiology	Microbiology	NNP
cord-342785-55r01n0x	185	3	and	and	CC
cord-342785-55r01n0x	185	4	Infectious	Infectious	NNP
cord-342785-55r01n0x	185	5	Disease	Disease	NNP
cord-342785-55r01n0x	185	6	Development	Development	NNP
cord-342785-55r01n0x	185	7	of	of	IN
cord-342785-55r01n0x	185	8	quantitative	quantitative	JJ
cord-342785-55r01n0x	185	9	gene	gene	NN
cord-342785-55r01n0x	185	10	-	-	HYPH
cord-342785-55r01n0x	185	11	specific	specific	JJ
cord-342785-55r01n0x	185	12	real	real	JJ
cord-342785-55r01n0x	185	13	-	-	HYPH
cord-342785-55r01n0x	185	14	time	time	NN
cord-342785-55r01n0x	185	15	RT	rt	NN
cord-342785-55r01n0x	185	16	-	-	HYPH
cord-342785-55r01n0x	185	17	PCR	PCR	NNP
cord-342785-55r01n0x	185	18	assays	assay	NNS
cord-342785-55r01n0x	185	19	for	for	IN
cord-342785-55r01n0x	185	20	the	the	DT
cord-342785-55r01n0x	185	21	detection	detection	NN
cord-342785-55r01n0x	185	22	of	of	IN
cord-342785-55r01n0x	185	23	measles	measle	NNS
cord-342785-55r01n0x	185	24	virus	virus	NN
cord-342785-55r01n0x	185	25	in	in	IN
cord-342785-55r01n0x	185	26	clinical	clinical	JJ
cord-342785-55r01n0x	185	27	specimens	specimen	NNS
cord-342785-55r01n0x	185	28	Limitations	limitation	NNS
cord-342785-55r01n0x	185	29	of	of	IN
cord-342785-55r01n0x	185	30	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	185	31	PCR	PCR	NNP
cord-342785-55r01n0x	185	32	for	for	IN
cord-342785-55r01n0x	185	33	detecting	detect	VBG
cord-342785-55r01n0x	185	34	divergent	divergent	JJ
cord-342785-55r01n0x	185	35	viral	viral	JJ
cord-342785-55r01n0x	185	36	pathogens	pathogen	NNS
cord-342785-55r01n0x	185	37	illustrated	illustrate	VBN
cord-342785-55r01n0x	185	38	by	by	IN
cord-342785-55r01n0x	185	39	hepatitis	hepatitis	NNP
cord-342785-55r01n0x	185	40	A	A	NNP
cord-342785-55r01n0x	185	41	,	,	,
cord-342785-55r01n0x	185	42	B	B	NNP
cord-342785-55r01n0x	185	43	,	,	,
cord-342785-55r01n0x	185	44	C	C	NNP
cord-342785-55r01n0x	185	45	,	,	,
cord-342785-55r01n0x	185	46	and	and	CC
cord-342785-55r01n0x	185	47	E	e	NN
cord-342785-55r01n0x	185	48	viruses	virus	NNS
cord-342785-55r01n0x	185	49	and	and	CC
cord-342785-55r01n0x	185	50	human	human	JJ
cord-342785-55r01n0x	185	51	immunodeficiency	immunodeficiency	NN
cord-342785-55r01n0x	185	52	virus	virus	NN
cord-342785-55r01n0x	185	53	Development	Development	NNP
cord-342785-55r01n0x	185	54	of	of	IN
cord-342785-55r01n0x	185	55	Mulitplex	Mulitplex	NNP
cord-342785-55r01n0x	185	56	Real	Real	NNP
cord-342785-55r01n0x	185	57	-	-	HYPH
cord-342785-55r01n0x	185	58	Time	Time	NNP
cord-342785-55r01n0x	185	59	Reverse	Reverse	NNP
cord-342785-55r01n0x	185	60	Transcriptase	Transcriptase	NNP
cord-342785-55r01n0x	185	61	PCR	pcr	NN
cord-342785-55r01n0x	185	62	Assays	Assays	NNP
cord-342785-55r01n0x	185	63	for	for	IN
cord-342785-55r01n0x	185	64	Detecting	detect	VBG
cord-342785-55r01n0x	185	65	Eight	eight	CD
cord-342785-55r01n0x	185	66	Medically	medically	RB
cord-342785-55r01n0x	185	67	Important	important	JJ
cord-342785-55r01n0x	185	68	Flaviviruses	Flaviviruses	NNPS
cord-342785-55r01n0x	185	69	in	in	IN
cord-342785-55r01n0x	185	70	Mosquitoes	Mosquitoes	NNPS
cord-342785-55r01n0x	185	71	Development	Development	NNP
cord-342785-55r01n0x	185	72	of	of	IN
cord-342785-55r01n0x	185	73	Real	Real	NNP
cord-342785-55r01n0x	185	74	-	-	HYPH
cord-342785-55r01n0x	185	75	Time	time	NN
cord-342785-55r01n0x	185	76	Reverse	Reverse	NNP
cord-342785-55r01n0x	185	77	Transcriptase	Transcriptase	NNP
cord-342785-55r01n0x	185	78	PCR	pcr	NN
cord-342785-55r01n0x	185	79	Assays	assay	NNS
cord-342785-55r01n0x	186	1	To	to	TO
cord-342785-55r01n0x	186	2	Detect	detect	VB
cord-342785-55r01n0x	186	3	and	and	CC
cord-342785-55r01n0x	186	4	Serotype	Serotype	NNP
cord-342785-55r01n0x	186	5	Dengue	dengue	NN
cord-342785-55r01n0x	186	6	Viruses	Viruses	NNPS
cord-342785-55r01n0x	186	7	Comparative	Comparative	NNP
cord-342785-55r01n0x	186	8	genomics	genomic	NNS
cord-342785-55r01n0x	186	9	tools	tool	NNS
cord-342785-55r01n0x	186	10	applied	apply	VBN
cord-342785-55r01n0x	186	11	to	to	IN
cord-342785-55r01n0x	186	12	bioterrorism	bioterrorism	NN
cord-342785-55r01n0x	186	13	defense	defense	NN
cord-342785-55r01n0x	186	14	Rapid	Rapid	NNP
cord-342785-55r01n0x	186	15	Development	Development	NNP
cord-342785-55r01n0x	186	16	of	of	IN
cord-342785-55r01n0x	186	17	Nucleic	Nucleic	NNP
cord-342785-55r01n0x	186	18	Acid	Acid	NNP
cord-342785-55r01n0x	186	19	Diagnostics	Diagnostics	NNPS
cord-342785-55r01n0x	186	20	Sequencing	Sequencing	NNP
cord-342785-55r01n0x	186	21	needs	need	NNS
cord-342785-55r01n0x	186	22	for	for	IN
cord-342785-55r01n0x	186	23	viral	viral	JJ
cord-342785-55r01n0x	186	24	diagnostics	diagnostic	NNS
cord-342785-55r01n0x	186	25	Design	Design	NNP
cord-342785-55r01n0x	186	26	and	and	CC
cord-342785-55r01n0x	186	27	Validation	Validation	NNP
cord-342785-55r01n0x	186	28	of	of	IN
cord-342785-55r01n0x	186	29	an	an	DT
cord-342785-55r01n0x	186	30	H5	H5	NNP
cord-342785-55r01n0x	186	31	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	186	32	Real	real	JJ
cord-342785-55r01n0x	186	33	-	-	HYPH
cord-342785-55r01n0x	186	34	Time	Time	NNP
cord-342785-55r01n0x	186	35	One	one	CD
cord-342785-55r01n0x	186	36	-	-	HYPH
cord-342785-55r01n0x	186	37	Step	Step	NNP
cord-342785-55r01n0x	186	38	Reverse	Reverse	NNP
cord-342785-55r01n0x	186	39	Transcription	Transcription	NNP
cord-342785-55r01n0x	186	40	-	-	HYPH
cord-342785-55r01n0x	186	41	PCR	PCR	NNP
cord-342785-55r01n0x	186	42	and	and	CC
cord-342785-55r01n0x	186	43	Confirmatory	Confirmatory	NNP
cord-342785-55r01n0x	186	44	Assays	Assays	NNP
cord-342785-55r01n0x	186	45	for	for	IN
cord-342785-55r01n0x	186	46	Diagnosis	Diagnosis	NNP
cord-342785-55r01n0x	186	47	and	and	CC
cord-342785-55r01n0x	186	48	Verification	Verification	NNP
cord-342785-55r01n0x	186	49	of	of	IN
cord-342785-55r01n0x	186	50	Influenza	Influenza	NNP
cord-342785-55r01n0x	186	51	A	A	NNP
cord-342785-55r01n0x	186	52	Virus	virus	NN
cord-342785-55r01n0x	186	53	H5	h5	NN
cord-342785-55r01n0x	186	54	Infections	Infections	NNPS
cord-342785-55r01n0x	186	55	in	in	IN
cord-342785-55r01n0x	186	56	Humans	Humans	NNPS
cord-342785-55r01n0x	186	57	Lion	Lion	NNP
cord-342785-55r01n0x	186	58	T	T	NNP
cord-342785-55r01n0x	186	59	:	:	:
cord-342785-55r01n0x	186	60	Real	real	JJ
cord-342785-55r01n0x	186	61	-	-	HYPH
cord-342785-55r01n0x	186	62	Time	time	NN
cord-342785-55r01n0x	186	63	Quantitative	Quantitative	NNP
cord-342785-55r01n0x	186	64	PCR	PCR	NNP
cord-342785-55r01n0x	186	65	Assays	Assays	NNP
cord-342785-55r01n0x	186	66	for	for	IN
cord-342785-55r01n0x	186	67	Detection	Detection	NNP
cord-342785-55r01n0x	186	68	and	and	CC
cord-342785-55r01n0x	186	69	Monitoring	Monitoring	NNP
cord-342785-55r01n0x	186	70	of	of	IN
cord-342785-55r01n0x	186	71	Pathogenic	Pathogenic	NNP
cord-342785-55r01n0x	186	72	Human	Human	NNP
cord-342785-55r01n0x	186	73	Viruses	Viruses	NNPS
cord-342785-55r01n0x	186	74	in	in	IN
cord-342785-55r01n0x	186	75	Immunosuppressed	Immunosuppressed	NNP
cord-342785-55r01n0x	186	76	Pediatric	Pediatric	NNP
cord-342785-55r01n0x	186	77	Patients	patient	NNS
cord-342785-55r01n0x	187	1	Rapid	rapid	JJ
cord-342785-55r01n0x	187	2	reverse	reverse	JJ
cord-342785-55r01n0x	187	3	transcription	transcription	NN
cord-342785-55r01n0x	187	4	-	-	HYPH
cord-342785-55r01n0x	187	5	PCR	pcr	NN
cord-342785-55r01n0x	187	6	detection	detection	NN
cord-342785-55r01n0x	187	7	of	of	IN
cord-342785-55r01n0x	187	8	hepatitis	hepatitis	NN
cord-342785-55r01n0x	187	9	C	C	NNP
cord-342785-55r01n0x	187	10	virus	virus	NN
cord-342785-55r01n0x	187	11	RNA	RNA	NNP
cord-342785-55r01n0x	187	12	in	in	IN
cord-342785-55r01n0x	187	13	serum	serum	NN
cord-342785-55r01n0x	187	14	by	by	IN
cord-342785-55r01n0x	187	15	using	use	VBG
cord-342785-55r01n0x	187	16	the	the	DT
cord-342785-55r01n0x	187	17	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	187	18	fluorogenic	fluorogenic	JJ
cord-342785-55r01n0x	187	19	detection	detection	NN
cord-342785-55r01n0x	187	20	system	system	NN
cord-342785-55r01n0x	187	21	Rapid	Rapid	NNP
cord-342785-55r01n0x	187	22	Detection	Detection	NNP
cord-342785-55r01n0x	187	23	of	of	IN
cord-342785-55r01n0x	187	24	West	West	NNP
cord-342785-55r01n0x	187	25	Nile	Nile	NNP
cord-342785-55r01n0x	187	26	Virus	Virus	NNP
cord-342785-55r01n0x	187	27	from	from	IN
cord-342785-55r01n0x	187	28	Human	Human	NNP
cord-342785-55r01n0x	187	29	Clinical	Clinical	NNP
cord-342785-55r01n0x	187	30	Specimens	Specimens	NNPS
cord-342785-55r01n0x	187	31	,	,	,
cord-342785-55r01n0x	187	32	Field	field	NN
cord-342785-55r01n0x	187	33	-	-	HYPH
cord-342785-55r01n0x	187	34	Collected	collect	VBN
cord-342785-55r01n0x	187	35	Mosquitoes	Mosquitoes	NNPS
cord-342785-55r01n0x	187	36	,	,	,
cord-342785-55r01n0x	187	37	and	and	CC
cord-342785-55r01n0x	187	38	Avian	avian	JJ
cord-342785-55r01n0x	187	39	Samples	sample	NNS
cord-342785-55r01n0x	187	40	by	by	IN
cord-342785-55r01n0x	187	41	a	a	DT
cord-342785-55r01n0x	187	42	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	187	43	Reverse	Reverse	NNP
cord-342785-55r01n0x	187	44	Transcriptase	Transcriptase	NNP
cord-342785-55r01n0x	187	45	-	-	HYPH
cord-342785-55r01n0x	187	46	PCR	PCR	NNP
cord-342785-55r01n0x	187	47	Assay	Assay	NNP
cord-342785-55r01n0x	187	48	Development	Development	NNP
cord-342785-55r01n0x	187	49	of	of	IN
cord-342785-55r01n0x	187	50	a	a	DT
cord-342785-55r01n0x	187	51	Quantitative	Quantitative	NNP
cord-342785-55r01n0x	187	52	Real	Real	NNP
cord-342785-55r01n0x	187	53	-	-	HYPH
cord-342785-55r01n0x	187	54	Time	Time	NNP
cord-342785-55r01n0x	187	55	Detection	Detection	NNP
cord-342785-55r01n0x	187	56	Assay	Assay	NNP
cord-342785-55r01n0x	187	57	for	for	IN
cord-342785-55r01n0x	187	58	Hepatitis	Hepatitis	NNP
cord-342785-55r01n0x	187	59	B	B	NNP
cord-342785-55r01n0x	187	60	Virus	virus	NN
cord-342785-55r01n0x	187	61	DNA	DNA	NNP
cord-342785-55r01n0x	187	62	and	and	CC
cord-342785-55r01n0x	187	63	Comparison	comparison	NN
cord-342785-55r01n0x	187	64	with	with	IN
cord-342785-55r01n0x	187	65	Two	two	CD
cord-342785-55r01n0x	187	66	Commercial	Commercial	NNP
cord-342785-55r01n0x	187	67	Assays	assay	NNS
cord-342785-55r01n0x	187	68	Sensitive	sensitive	JJ
cord-342785-55r01n0x	187	69	and	and	CC
cord-342785-55r01n0x	187	70	accurate	accurate	JJ
cord-342785-55r01n0x	187	71	quantitation	quantitation	NN
cord-342785-55r01n0x	187	72	of	of	IN
cord-342785-55r01n0x	187	73	hepatitis	hepatitis	NN
cord-342785-55r01n0x	187	74	B	B	NNP
cord-342785-55r01n0x	187	75	virus	virus	NN
cord-342785-55r01n0x	187	76	DNA	dna	NN
cord-342785-55r01n0x	187	77	using	use	VBG
cord-342785-55r01n0x	187	78	a	a	DT
cord-342785-55r01n0x	187	79	kinetic	kinetic	JJ
cord-342785-55r01n0x	187	80	fluorescence	fluorescence	NN
cord-342785-55r01n0x	187	81	detection	detection	NN
cord-342785-55r01n0x	187	82	system	system	NN
cord-342785-55r01n0x	187	83	(	(	-LRB-
cord-342785-55r01n0x	187	84	TagMan	TagMan	NNP
cord-342785-55r01n0x	187	85	PCR	PCR	NNP
cord-342785-55r01n0x	187	86	)	)	-RRB-
cord-342785-55r01n0x	188	1	Comparison	comparison	NN
cord-342785-55r01n0x	188	2	of	of	IN
cord-342785-55r01n0x	188	3	two	two	CD
cord-342785-55r01n0x	188	4	quantitative	quantitative	JJ
cord-342785-55r01n0x	188	5	CMV	CMV	NNP
cord-342785-55r01n0x	188	6	PCR	PCR	NNP
cord-342785-55r01n0x	188	7	tests	test	NNS
cord-342785-55r01n0x	188	8	,	,	,
cord-342785-55r01n0x	188	9	Cobas	Cobas	NNP
cord-342785-55r01n0x	188	10	Amplicor	Amplicor	NNP
cord-342785-55r01n0x	188	11	CMV	CMV	NNP
cord-342785-55r01n0x	188	12	Monitor	Monitor	NNP
cord-342785-55r01n0x	188	13	and	and	CC
cord-342785-55r01n0x	188	14	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	188	15	assay	assay	NN
cord-342785-55r01n0x	188	16	,	,	,
cord-342785-55r01n0x	188	17	and	and	CC
cord-342785-55r01n0x	188	18	pp65-antigenemia	pp65-antigenemia	NNP
cord-342785-55r01n0x	188	19	assay	assay	NN
cord-342785-55r01n0x	188	20	in	in	IN
cord-342785-55r01n0x	188	21	the	the	DT
cord-342785-55r01n0x	188	22	determination	determination	NN
cord-342785-55r01n0x	188	23	of	of	IN
cord-342785-55r01n0x	188	24	viral	viral	JJ
cord-342785-55r01n0x	188	25	loads	load	NNS
cord-342785-55r01n0x	188	26	from	from	IN
cord-342785-55r01n0x	188	27	peripheral	peripheral	JJ
cord-342785-55r01n0x	188	28	blood	blood	NN
cord-342785-55r01n0x	188	29	of	of	IN
cord-342785-55r01n0x	188	30	organ	organ	NN
cord-342785-55r01n0x	188	31	transplant	transplant	NN
cord-342785-55r01n0x	188	32	patients	patient	NNS
cord-342785-55r01n0x	188	33	Differentiation	differentiation	NN
cord-342785-55r01n0x	188	34	of	of	IN
cord-342785-55r01n0x	188	35	herpes	herpes	NN
cord-342785-55r01n0x	188	36	simplex	simplex	NN
cord-342785-55r01n0x	188	37	virus	virus	NN
cord-342785-55r01n0x	188	38	types	type	NNS
cord-342785-55r01n0x	188	39	1	1	CD
cord-342785-55r01n0x	188	40	and	and	CC
cord-342785-55r01n0x	188	41	2	2	CD
cord-342785-55r01n0x	188	42	in	in	IN
cord-342785-55r01n0x	188	43	clinical	clinical	JJ
cord-342785-55r01n0x	188	44	samples	sample	NNS
cord-342785-55r01n0x	188	45	by	by	IN
cord-342785-55r01n0x	188	46	a	a	DT
cord-342785-55r01n0x	188	47	real	real	JJ
cord-342785-55r01n0x	188	48	-	-	HYPH
cord-342785-55r01n0x	188	49	time	time	NN
cord-342785-55r01n0x	188	50	taqman	taqman	NNP
cord-342785-55r01n0x	188	51	PCR	PCR	NNP
cord-342785-55r01n0x	188	52	assay	assay	NN
cord-342785-55r01n0x	188	53	Development	Development	NNP
cord-342785-55r01n0x	188	54	of	of	IN
cord-342785-55r01n0x	188	55	a	a	DT
cord-342785-55r01n0x	188	56	flurogenic	flurogenic	JJ
cord-342785-55r01n0x	188	57	polymerase	polymerase	NN
cord-342785-55r01n0x	188	58	chain	chain	NN
cord-342785-55r01n0x	188	59	reaction	reaction	NN
cord-342785-55r01n0x	188	60	assay	assay	NN
cord-342785-55r01n0x	188	61	(	(	-LRB-
cord-342785-55r01n0x	188	62	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	188	63	)	)	-RRB-
cord-342785-55r01n0x	188	64	for	for	IN
cord-342785-55r01n0x	188	65	the	the	DT
cord-342785-55r01n0x	188	66	detection	detection	NN
cord-342785-55r01n0x	188	67	and	and	CC
cord-342785-55r01n0x	188	68	quantitation	quantitation	NN
cord-342785-55r01n0x	188	69	of	of	IN
cord-342785-55r01n0x	188	70	varicella	varicella	NN
cord-342785-55r01n0x	188	71	zoster	zoster	NN
cord-342785-55r01n0x	188	72	virus	virus	NN
cord-342785-55r01n0x	188	73	Rapid	Rapid	NNP
cord-342785-55r01n0x	188	74	and	and	CC
cord-342785-55r01n0x	188	75	sensitive	sensitive	JJ
cord-342785-55r01n0x	188	76	detection	detection	NN
cord-342785-55r01n0x	188	77	of	of	IN
cord-342785-55r01n0x	188	78	mumps	mumps	NN
cord-342785-55r01n0x	188	79	virus	virus	NN
cord-342785-55r01n0x	188	80	RNA	RNA	NNP
cord-342785-55r01n0x	188	81	directly	directly	RB
cord-342785-55r01n0x	188	82	from	from	IN
cord-342785-55r01n0x	188	83	clinical	clinical	JJ
cord-342785-55r01n0x	188	84	samples	sample	NNS
cord-342785-55r01n0x	188	85	by	by	IN
cord-342785-55r01n0x	188	86	real	real	JJ
cord-342785-55r01n0x	188	87	-	-	HYPH
cord-342785-55r01n0x	188	88	time	time	NN
cord-342785-55r01n0x	188	89	PCR	PCR	NNP
cord-342785-55r01n0x	188	90	Development	Development	NNP
cord-342785-55r01n0x	188	91	of	of	IN
cord-342785-55r01n0x	188	92	a	a	DT
cord-342785-55r01n0x	188	93	Real	Real	NNP
cord-342785-55r01n0x	188	94	-	-	HYPH
cord-342785-55r01n0x	188	95	Time	time	NN
cord-342785-55r01n0x	188	96	Reverse	reverse	NN
cord-342785-55r01n0x	188	97	-	-	HYPH
cord-342785-55r01n0x	188	98	Transcription	transcription	NN
cord-342785-55r01n0x	188	99	PCR	PCR	NNP
cord-342785-55r01n0x	188	100	for	for	IN
cord-342785-55r01n0x	188	101	Detection	Detection	NNP
cord-342785-55r01n0x	188	102	of	of	IN
cord-342785-55r01n0x	188	103	Newcastle	Newcastle	NNP
cord-342785-55r01n0x	188	104	Disease	Disease	NNP
cord-342785-55r01n0x	188	105	Virus	Virus	NNP
cord-342785-55r01n0x	188	106	RNA	RNA	NNP
cord-342785-55r01n0x	188	107	in	in	IN
cord-342785-55r01n0x	188	108	Clinical	Clinical	NNP
cord-342785-55r01n0x	188	109	Samples	Samples	NNPS
cord-342785-55r01n0x	188	110	Transfer	Transfer	NNP
cord-342785-55r01n0x	188	111	and	and	CC
cord-342785-55r01n0x	188	112	Evaluation	Evaluation	NNP
cord-342785-55r01n0x	188	113	of	of	IN
cord-342785-55r01n0x	188	114	an	an	DT
cord-342785-55r01n0x	188	115	Automated	Automated	NNP
cord-342785-55r01n0x	188	116	,	,	,
cord-342785-55r01n0x	188	117	Low	low	JJ
cord-342785-55r01n0x	188	118	-	-	HYPH
cord-342785-55r01n0x	188	119	Cost	cost	NN
cord-342785-55r01n0x	188	120	Real	real	JJ
cord-342785-55r01n0x	188	121	-	-	HYPH
cord-342785-55r01n0x	188	122	Time	time	NN
cord-342785-55r01n0x	188	123	Reverse	Reverse	NNP
cord-342785-55r01n0x	188	124	Transcription	Transcription	NNP
cord-342785-55r01n0x	188	125	-	-	HYPH
cord-342785-55r01n0x	188	126	PCR	PCR	NNP
cord-342785-55r01n0x	188	127	Test	test	NN
cord-342785-55r01n0x	188	128	for	for	IN
cord-342785-55r01n0x	188	129	Diagnosis	Diagnosis	NNP
cord-342785-55r01n0x	188	130	and	and	CC
cord-342785-55r01n0x	188	131	Monitoring	Monitoring	NNP
cord-342785-55r01n0x	188	132	of	of	IN
cord-342785-55r01n0x	188	133	Human	Human	NNP
cord-342785-55r01n0x	188	134	Immunodeficiency	Immunodeficiency	NNP
cord-342785-55r01n0x	188	135	Virus	Virus	NNP
cord-342785-55r01n0x	188	136	Type	Type	NNP
cord-342785-55r01n0x	188	137	1	1	CD
cord-342785-55r01n0x	188	138	Infection	infection	NN
cord-342785-55r01n0x	188	139	in	in	IN
cord-342785-55r01n0x	188	140	a	a	DT
cord-342785-55r01n0x	188	141	West	west	JJ
cord-342785-55r01n0x	188	142	African	african	JJ
cord-342785-55r01n0x	188	143	Resource	resource	NN
cord-342785-55r01n0x	188	144	-	-	HYPH
cord-342785-55r01n0x	188	145	Limited	Limited	NNP
cord-342785-55r01n0x	188	146	Setting	set	VBG
cord-342785-55r01n0x	188	147	Rapid	Rapid	NNP
cord-342785-55r01n0x	188	148	Detection	Detection	NNP
cord-342785-55r01n0x	188	149	of	of	IN
cord-342785-55r01n0x	188	150	Enterovirus	Enterovirus	NNP
cord-342785-55r01n0x	188	151	RNA	RNA	NNP
cord-342785-55r01n0x	188	152	in	in	IN
cord-342785-55r01n0x	188	153	Cerebrospinal	Cerebrospinal	NNP
cord-342785-55r01n0x	188	154	Fluid	Fluid	NNP
cord-342785-55r01n0x	188	155	Specimens	Specimens	NNP
cord-342785-55r01n0x	188	156	with	with	IN
cord-342785-55r01n0x	188	157	a	a	DT
cord-342785-55r01n0x	188	158	Novel	Novel	NNP
cord-342785-55r01n0x	188	159	Single	Single	NNP
cord-342785-55r01n0x	188	160	-	-	HYPH
cord-342785-55r01n0x	188	161	Tube	tube	NN
cord-342785-55r01n0x	188	162	Real	real	JJ
cord-342785-55r01n0x	188	163	-	-	HYPH
cord-342785-55r01n0x	188	164	Time	time	NN
cord-342785-55r01n0x	188	165	Reverse	Reverse	NNP
cord-342785-55r01n0x	188	166	Transcription	Transcription	NNP
cord-342785-55r01n0x	188	167	-	-	HYPH
cord-342785-55r01n0x	188	168	PCR	PCR	NNP
cord-342785-55r01n0x	188	169	Assay	Assay	NNP
cord-342785-55r01n0x	188	170	Use	Use	NNP
cord-342785-55r01n0x	188	171	of	of	IN
cord-342785-55r01n0x	188	172	Applied	Applied	NNP
cord-342785-55r01n0x	188	173	Biosystems	Biosystems	NNP
cord-342785-55r01n0x	188	174	7900HT	7900ht	CD
cord-342785-55r01n0x	188	175	Sequence	Sequence	NNP
cord-342785-55r01n0x	188	176	Detection	Detection	NNP
cord-342785-55r01n0x	188	177	System	System	NNP
cord-342785-55r01n0x	188	178	and	and	CC
cord-342785-55r01n0x	188	179	Taqman	Taqman	NNP
cord-342785-55r01n0x	188	180	Assay	Assay	NNP
cord-342785-55r01n0x	188	181	for	for	IN
cord-342785-55r01n0x	188	182	Detection	detection	NN
cord-342785-55r01n0x	188	183	of	of	IN
cord-342785-55r01n0x	188	184	Quinolone	Quinolone	NNP
cord-342785-55r01n0x	188	185	-	-	HYPH
cord-342785-55r01n0x	188	186	Resistant	Resistant	NNP
cord-342785-55r01n0x	188	187	Neisseria	Neisseria	NNP
cord-342785-55r01n0x	188	188	gonorrhoeae	gonorrhoeae	NN
cord-342785-55r01n0x	188	189	Comparison	comparison	NN
cord-342785-55r01n0x	188	190	of	of	IN
cord-342785-55r01n0x	188	191	a	a	DT
cord-342785-55r01n0x	188	192	New	New	NNP
cord-342785-55r01n0x	188	193	Quantitative	Quantitative	NNP
cord-342785-55r01n0x	188	194	ompA	ompA	NNP
cord-342785-55r01n0x	188	195	-	-	HYPH
cord-342785-55r01n0x	188	196	Based	base	VBN
cord-342785-55r01n0x	188	197	Real	real	JJ
cord-342785-55r01n0x	188	198	-	-	HYPH
cord-342785-55r01n0x	188	199	time	time	NN
cord-342785-55r01n0x	188	200	PCR	PCR	NNP
cord-342785-55r01n0x	188	201	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	188	202	Assay	Assay	NNP
cord-342785-55r01n0x	188	203	for	for	IN
cord-342785-55r01n0x	188	204	Detection	detection	NN
cord-342785-55r01n0x	188	205	of	of	IN
cord-342785-55r01n0x	188	206	Chlamydia	Chlamydia	NNP
cord-342785-55r01n0x	188	207	pneumoniae	pneumoniae	NN
cord-342785-55r01n0x	188	208	DNA	dna	NN
cord-342785-55r01n0x	188	209	in	in	IN
cord-342785-55r01n0x	188	210	Respiratory	respiratory	JJ
cord-342785-55r01n0x	188	211	Specimens	specimen	NNS
cord-342785-55r01n0x	188	212	with	with	IN
cord-342785-55r01n0x	188	213	Four	four	CD
cord-342785-55r01n0x	188	214	Conventional	Conventional	NNP
cord-342785-55r01n0x	188	215	PCR	pcr	NN
cord-342785-55r01n0x	188	216	Assays	Assays	NNP
cord-342785-55r01n0x	188	217	A	a	DT
cord-342785-55r01n0x	188	218	LightCycler	LightCycler	NNP
cord-342785-55r01n0x	188	219	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	188	220	assay	assay	NN
cord-342785-55r01n0x	188	221	for	for	IN
cord-342785-55r01n0x	188	222	detection	detection	NN
cord-342785-55r01n0x	188	223	of	of	IN
cord-342785-55r01n0x	188	224	Borrelia	Borrelia	NNP
cord-342785-55r01n0x	188	225	burgdorferi	burgdorferi	NN
cord-342785-55r01n0x	188	226	sensu	sensu	NNP
cord-342785-55r01n0x	188	227	lato	lato	NNP
cord-342785-55r01n0x	188	228	in	in	IN
cord-342785-55r01n0x	188	229	clinical	clinical	JJ
cord-342785-55r01n0x	188	230	samples	sample	NNS
cord-342785-55r01n0x	188	231	Detection	detection	NN
cord-342785-55r01n0x	188	232	of	of	IN
cord-342785-55r01n0x	188	233	Medically	medically	RB
cord-342785-55r01n0x	188	234	Important	important	JJ
cord-342785-55r01n0x	188	235	Ehrlichia	Ehrlichia	NNP
cord-342785-55r01n0x	188	236	by	by	IN
cord-342785-55r01n0x	188	237	Quantitative	Quantitative	NNP
cord-342785-55r01n0x	188	238	Multicolor	Multicolor	NNP
cord-342785-55r01n0x	188	239	TaqMan	TaqMan	NNP
cord-342785-55r01n0x	188	240	Real	real	JJ
cord-342785-55r01n0x	188	241	-	-	HYPH
cord-342785-55r01n0x	188	242	Time	time	NN
cord-342785-55r01n0x	188	243	Polymerase	Polymerase	NNP
cord-342785-55r01n0x	188	244	Chain	Chain	NNP
cord-342785-55r01n0x	188	245	Reaction	reaction	NN
cord-342785-55r01n0x	188	246	of	of	IN
cord-342785-55r01n0x	188	247	the	the	DT
cord-342785-55r01n0x	188	248	dsb	dsb	NNP
cord-342785-55r01n0x	188	249	Gene	Gene	NNP
cord-342785-55r01n0x	189	1	We	-PRON-	PRP
cord-342785-55r01n0x	189	2	thank	thank	VBP
cord-342785-55r01n0x	189	3	Beth	Beth	NNP
cord-342785-55r01n0x	189	4	Vitalis	Vitalis	NNP
cord-342785-55r01n0x	189	5	,	,	,
cord-342785-55r01n0x	189	6	Jason	Jason	NNP
cord-342785-55r01n0x	189	7	Smith	Smith	NNP
cord-342785-55r01n0x	189	8	,	,	,
cord-342785-55r01n0x	189	9	and	and	CC
cord-342785-55r01n0x	189	10	Tom	Tom	NNP
cord-342785-55r01n0x	189	11	Slezak	Slezak	NNP
cord-342785-55r01n0x	189	12	for	for	IN
cord-342785-55r01n0x	189	13	helpful	helpful	JJ
cord-342785-55r01n0x	189	14	discussion	discussion	NN
cord-342785-55r01n0x	189	15	and	and	CC
cord-342785-55r01n0x	189	16	for	for	IN
cord-342785-55r01n0x	189	17	encouraging	encourage	VBG
cord-342785-55r01n0x	189	18	this	this	DT
cord-342785-55r01n0x	189	19	work	work	NN
cord-342785-55r01n0x	189	20	,	,	,
cord-342785-55r01n0x	189	21	and	and	CC
cord-342785-55r01n0x	189	22	Kari	Kari	NNP
cord-342785-55r01n0x	189	23	Allmon	Allmon	NNP
cord-342785-55r01n0x	189	24	for	for	IN
cord-342785-55r01n0x	189	25	entering	enter	VBG
cord-342785-55r01n0x	189	26	the	the	DT
cord-342785-55r01n0x	189	27	references	reference	NNS
cord-342785-55r01n0x	189	28	.	.	.
cord-342785-55r01n0x	190	1	We	-PRON-	PRP
cord-342785-55r01n0x	190	2	gratefully	gratefully	RB
cord-342785-55r01n0x	190	3	acknowledge	acknowledge	VBP
cord-342785-55r01n0x	190	4	financial	financial	JJ
cord-342785-55r01n0x	190	5	support	support	NN
cord-342785-55r01n0x	190	6	from	from	IN
cord-342785-55r01n0x	190	7	the	the	DT
cord-342785-55r01n0x	190	8	Intelligence	Intelligence	NNP
cord-342785-55r01n0x	190	9	Technology	Technology	NNP
cord-342785-55r01n0x	190	10	Innovation	Innovation	NNP
cord-342785-55r01n0x	190	11	Center	Center	NNP
cord-342785-55r01n0x	190	12	.	.	.
cord-342785-55r01n0x	191	1	Lawrence	Lawrence	NNP
cord-342785-55r01n0x	191	2	Livermore	Livermore	NNP
cord-342785-55r01n0x	191	3	National	National	NNP
cord-342785-55r01n0x	191	4	Laboratory	Laboratory	NNP
cord-342785-55r01n0x	191	5	is	be	VBZ
cord-342785-55r01n0x	191	6	operated	operate	VBN
cord-342785-55r01n0x	191	7	by	by	IN
cord-342785-55r01n0x	191	8	Lawrence	Lawrence	NNP
cord-342785-55r01n0x	191	9	Livermore	Livermore	NNP
cord-342785-55r01n0x	191	10	National	National	NNP
cord-342785-55r01n0x	191	11	Security	Security	NNP
cord-342785-55r01n0x	191	12	,	,	,
cord-342785-55r01n0x	191	13	LLC	LLC	NNP
cord-342785-55r01n0x	191	14	,	,	,
cord-342785-55r01n0x	191	15	for	for	IN
cord-342785-55r01n0x	191	16	the	the	DT
cord-342785-55r01n0x	191	17	U.S.	U.S.	NNP
cord-342785-55r01n0x	191	18	Department	Department	NNP
cord-342785-55r01n0x	191	19	of	of	IN
cord-342785-55r01n0x	191	20	Energy	Energy	NNP
cord-342785-55r01n0x	191	21	,	,	,
cord-342785-55r01n0x	191	22	National	National	NNP
cord-342785-55r01n0x	191	23	Nuclear	Nuclear	NNP
cord-342785-55r01n0x	191	24	Security	Security	NNP
cord-342785-55r01n0x	191	25	Administration	Administration	NNP
cord-342785-55r01n0x	191	26	under	under	IN
cord-342785-55r01n0x	191	27	Contract	Contract	NNP
cord-342785-55r01n0x	191	28	DE	DE	NNP
cord-342785-55r01n0x	191	29	-	-	HYPH
cord-342785-55r01n0x	191	30	AC52	AC52	NNP
cord-342785-55r01n0x	191	31	-	-	HYPH
cord-342785-55r01n0x	191	32	07NA27344	07na27344	CD
cord-342785-55r01n0x	191	33	.	.	.
cord-342785-55r01n0x	192	1	The	the	DT
cord-342785-55r01n0x	192	2	authors	author	NNS
cord-342785-55r01n0x	192	3	declare	declare	VBP
cord-342785-55r01n0x	192	4	that	that	IN
cord-342785-55r01n0x	192	5	they	-PRON-	PRP
cord-342785-55r01n0x	192	6	have	have	VBP
cord-342785-55r01n0x	192	7	no	no	DT
cord-342785-55r01n0x	192	8	competing	compete	VBG
cord-342785-55r01n0x	192	9	interests	interest	NNS
cord-342785-55r01n0x	192	10	.	.	.
cord-342785-55r01n0x	193	1	GL	GL	NNP
cord-342785-55r01n0x	193	2	found	find	VBD
cord-342785-55r01n0x	193	3	real	real	JJ
cord-342785-55r01n0x	193	4	time	time	NN
cord-342785-55r01n0x	193	5	PCR	PCR	NNP
cord-342785-55r01n0x	193	6	signatures	signature	NNS
cord-342785-55r01n0x	193	7	in	in	IN
cord-342785-55r01n0x	193	8	the	the	DT
cord-342785-55r01n0x	193	9	literature	literature	NN
cord-342785-55r01n0x	193	10	,	,	,
cord-342785-55r01n0x	193	11	wrote	write	VBD
cord-342785-55r01n0x	193	12	Perl	Perl	NNP
cord-342785-55r01n0x	193	13	scripts	script	NNS
cord-342785-55r01n0x	193	14	,	,	,
cord-342785-55r01n0x	193	15	and	and	CC
cord-342785-55r01n0x	193	16	performed	perform	VBD
cord-342785-55r01n0x	193	17	the	the	DT
cord-342785-55r01n0x	193	18	analysis	analysis	NN
cord-342785-55r01n0x	193	19	of	of	IN
cord-342785-55r01n0x	193	20	published	publish	VBN
cord-342785-55r01n0x	193	21	signatures	signature	NNS
cord-342785-55r01n0x	193	22	.	.	.
cord-342785-55r01n0x	194	1	SG	SG	NNP
cord-342785-55r01n0x	194	2	conceived	conceive	VBN
cord-342785-55r01n0x	194	3	of	of	IN
cord-342785-55r01n0x	194	4	the	the	DT
cord-342785-55r01n0x	194	5	research	research	NN
cord-342785-55r01n0x	194	6	,	,	,
cord-342785-55r01n0x	194	7	designed	design	VBN
cord-342785-55r01n0x	194	8	new	new	JJ
cord-342785-55r01n0x	194	9	signatures	signature	NNS
cord-342785-55r01n0x	194	10	,	,	,
cord-342785-55r01n0x	194	11	and	and	CC
cord-342785-55r01n0x	194	12	provided	provide	VBN
cord-342785-55r01n0x	194	13	guidance	guidance	NN
cord-342785-55r01n0x	194	14	throughout	throughout	IN
cord-342785-55r01n0x	194	15	the	the	DT
cord-342785-55r01n0x	194	16	study	study	NN
cord-342785-55r01n0x	194	17	.	.	.
cord-342785-55r01n0x	195	1	Publish	publish	VB
cord-342785-55r01n0x	195	2	with	with	IN
cord-342785-55r01n0x	195	3	Bio	Bio	NNP
cord-342785-55r01n0x	195	4	Med	Med	NNP
cord-342785-55r01n0x	195	5	Central	Central	NNP
cord-342785-55r01n0x	195	6	and	and	CC
cord-342785-55r01n0x	195	7	every	every	DT
cord-342785-55r01n0x	195	8	scientist	scientist	NN
cord-342785-55r01n0x	195	9	can	can	MD
cord-342785-55r01n0x	195	10	read	read	VB
cord-342785-55r01n0x	195	11	your	-PRON-	PRP$
cord-342785-55r01n0x	195	12	work	work	NN
cord-342785-55r01n0x	195	13	free	free	JJ
cord-342785-55r01n0x	195	14	of	of	IN
cord-342785-55r01n0x	195	15	charge	charge	NN
cord-342785-55r01n0x	195	16	http://www.ann-clinmicrob.com/content/7/1/18	http://www.ann-clinmicrob.com/content/7/1/18	.