id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007613-g4s0v8ra	Rimstad, Espen	Cloning, expression and characterization of biologically active feline tumour necrosis factor-α	2000-03-10		.txt	text/plain	4664	265	58	To assure that stimulated macrophage mRNA was the actual source for cDNA, cDNA derived by reverse transcription of mRNA from unstimulated macrophage cultures, as well as feline genomic DNA, were extracted and used as targets in separate and parallel PCRs. The amplified fragments generated by both the P 1 /P3 and P2/P3 primers were separately cloned into the plasmid vector pCRI1 (TA-cloning kit, Invitrogen), and the nucleotide sequences were determined by conventional dideoxy sequencing of both strands. Although some variation was evident between the four individual donor cats, rfTNF-a induced a dose related increase of IG2R and MHC class II antigen expression on the cell surface of in vitro stimulated feline PMBCs (Fig. 6 ) . Tumor necrosis factor a induces expression of human immunodeticiency virus in a chronically infected T-cell clone Tumor necrosis factor a induces proteins that bind specifically to kB-like enhancer elements and regulate interleukin 2 receptor a-chain gene expression in primary human T-lymphocytes	./cache/cord-007613-g4s0v8ra.txt	./txt/cord-007613-g4s0v8ra.txt