id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-007621-rapinodd	Vidovic, Maria	Induction and regulation of class II major histocompatibility complex mRNA expression in astrocytes by interferon-γ and tumor necrosis factor-α	2002-11-13		.txt	text/plain	6680	365	57	Previous data from this laboratory had shown that the cytokine tumor necrosis factor-α (TNF-α) enhances IFN-γ-mediated class II antigen expression on astrocytes. To determine the steady-state level of mRNA for class II, Northern blot analysis was performed using a eDNA probe for murine class Ii genes (E-a), with total RNA isolated from cultured astrocytes. The duration of protein synthesis required to allow expression of the class II MHC gene in astrocytes was examined in cells that were pretreated with IFN-y or IFN-7/TNF-a for different lengths of time prior to the addition of CHX. In this study we have shown that primary neonatal rat astrocytes, upon stimulation with IFN-~,, express mRNA transcripts for class II MHC genes, and that TNF-a enhances the expression of IFN-~,-induced class II mRNA. The expression of class II mRNA was completely inhibited when CHX was included with IFN-~, and IFN-'t/TNF-~ treatment, indicating that newly synthesized protein is required for astrocyte class II MHC gene expression.	./cache/cord-007621-rapinodd.txt	./txt/cord-007621-rapinodd.txt