id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-301102-jbjysyqm	Priestnall, Simon L.	Quantification of mRNA encoding cytokines and chemokines and assessment of ciliary function in canine tracheal epithelium during infection with canine respiratory coronavirus (CRCoV)	2009-01-15		.txt	text/plain	6022	296	48	title: Quantification of mRNA encoding cytokines and chemokines and assessment of ciliary function in canine tracheal epithelium during infection with canine respiratory coronavirus (CRCoV) This study aimed to quantify pro-inflammatory cytokine mRNAs following infection of canine air-interface tracheal cultures with CRCoV. This study aimed to quantify pro-inflammatory cytokine mRNAs following infection of canine air-interface tracheal cultures with CRCoV. The quantification of canine IL-6, IL-8 and TNF-a mRNA copies in CRCoV-inoculated cultures was presented as the logarithm of the fold change relative to control-inoculated cultures in the same dog at the same time point. In response to LPS, mRNA levels of TNF-a, IL-6 and IL-8 in cultures, were significantly increased from 24 h post-inoculation, relative to controls, indicating that the assay was sensitive enough to detect changes in cytokine mRNAs within this system. IHC revealed coronavirus antigen positive intra-cytoplasmic staining of ciliated epithelial and goblet cells within canine tracheas of both CRCoV-inoculated cultures and from naturally infected cases of CIRD.	./cache/cord-301102-jbjysyqm.txt	./txt/cord-301102-jbjysyqm.txt