id sid tid token lemma pos cord-275413-e2rhioty 1 1 key key JJ cord-275413-e2rhioty 1 2 : : : cord-275413-e2rhioty 1 3 cord-275413-e2rhioty cord-275413-e2rhioty NNP cord-275413-e2rhioty 1 4 authors author NNS cord-275413-e2rhioty 1 5 : : : cord-275413-e2rhioty 1 6 Rowland Rowland NNP cord-275413-e2rhioty 1 7 , , , cord-275413-e2rhioty 1 8 Raymond Raymond NNP cord-275413-e2rhioty 1 9 R.R. R.R. NNP cord-275413-e2rhioty 1 10 title title NN cord-275413-e2rhioty 1 11 : : : cord-275413-e2rhioty 2 1 The the DT cord-275413-e2rhioty 2 2 interaction interaction NN cord-275413-e2rhioty 2 3 between between IN cord-275413-e2rhioty 2 4 PRRSV PRRSV NNP cord-275413-e2rhioty 2 5 and and CC cord-275413-e2rhioty 2 6 the the DT cord-275413-e2rhioty 2 7 late late JJ cord-275413-e2rhioty 2 8 gestation gestation NN cord-275413-e2rhioty 2 9 pig pig NN cord-275413-e2rhioty 2 10 fetus fetus NN cord-275413-e2rhioty 2 11 date date NN cord-275413-e2rhioty 2 12 : : : cord-275413-e2rhioty 2 13 2010 2010 CD cord-275413-e2rhioty 2 14 - - SYM cord-275413-e2rhioty 2 15 09 09 CD cord-275413-e2rhioty 2 16 - - HYPH cord-275413-e2rhioty 2 17 09 09 CD cord-275413-e2rhioty 2 18 journal journal NN cord-275413-e2rhioty 2 19 : : : cord-275413-e2rhioty 3 1 Virus virus NN cord-275413-e2rhioty 3 2 Res Res NNP cord-275413-e2rhioty 3 3 DOI doi NN cord-275413-e2rhioty 3 4 : : : cord-275413-e2rhioty 3 5 10.1016/ 10.1016/ CD cord-275413-e2rhioty 4 1 j.virusres.2010.09.001 j.virusres.2010.09.001 NNP cord-275413-e2rhioty 4 2 sha sha NNP cord-275413-e2rhioty 4 3 : : : cord-275413-e2rhioty 5 1 e6e47cc241431c90ddcefa5583b526a7bf4cab0d e6e47cc241431c90ddcefa5583b526a7bf4cab0d NNP cord-275413-e2rhioty 5 2 doc_id doc_id NNPS cord-275413-e2rhioty 5 3 : : : cord-275413-e2rhioty 6 1 275413 275413 CD cord-275413-e2rhioty 7 1 cord_uid cord_uid NN cord-275413-e2rhioty 7 2 : : : cord-275413-e2rhioty 8 1 e2rhioty e2rhioty NNP cord-275413-e2rhioty 8 2 Porcine porcine JJ cord-275413-e2rhioty 8 3 reproductive reproductive JJ cord-275413-e2rhioty 8 4 and and CC cord-275413-e2rhioty 8 5 respiratory respiratory JJ cord-275413-e2rhioty 8 6 syndrome syndrome NN cord-275413-e2rhioty 8 7 virus virus NN cord-275413-e2rhioty 8 8 ( ( -LRB- cord-275413-e2rhioty 8 9 PRRSV PRRSV NNP cord-275413-e2rhioty 8 10 ) ) -RRB- cord-275413-e2rhioty 8 11 crosses cross VBZ cord-275413-e2rhioty 8 12 the the DT cord-275413-e2rhioty 8 13 placenta placenta NN cord-275413-e2rhioty 8 14 during during IN cord-275413-e2rhioty 8 15 late late JJ cord-275413-e2rhioty 8 16 gestation gestation NN cord-275413-e2rhioty 8 17 and and CC cord-275413-e2rhioty 8 18 productively productively RB cord-275413-e2rhioty 8 19 infects infect VBZ cord-275413-e2rhioty 8 20 the the DT cord-275413-e2rhioty 8 21 fetus fetus NN cord-275413-e2rhioty 8 22 . . . cord-275413-e2rhioty 9 1 Virus virus NN cord-275413-e2rhioty 9 2 replication replication NN cord-275413-e2rhioty 9 3 and and CC cord-275413-e2rhioty 9 4 cytokine cytokine NN cord-275413-e2rhioty 9 5 responses response NNS cord-275413-e2rhioty 9 6 were be VBD cord-275413-e2rhioty 9 7 measured measure VBN cord-275413-e2rhioty 9 8 in in IN cord-275413-e2rhioty 9 9 tissues tissue NNS cord-275413-e2rhioty 9 10 of of IN cord-275413-e2rhioty 9 11 fetuses fetus NNS cord-275413-e2rhioty 9 12 recovered recover VBN cord-275413-e2rhioty 9 13 at at IN cord-275413-e2rhioty 9 14 109–112 109–112 CD cord-275413-e2rhioty 9 15 days day NNS cord-275413-e2rhioty 9 16 of of IN cord-275413-e2rhioty 9 17 gestation gestation NN cord-275413-e2rhioty 9 18 , , , cord-275413-e2rhioty 9 19 just just RB cord-275413-e2rhioty 9 20 prior prior RB cord-275413-e2rhioty 9 21 to to IN cord-275413-e2rhioty 9 22 parturition parturition NN cord-275413-e2rhioty 9 23 . . . cord-275413-e2rhioty 10 1 At at IN cord-275413-e2rhioty 10 2 the the DT cord-275413-e2rhioty 10 3 time time NN cord-275413-e2rhioty 10 4 of of IN cord-275413-e2rhioty 10 5 recovery recovery NN cord-275413-e2rhioty 10 6 , , , cord-275413-e2rhioty 10 7 gross gross JJ cord-275413-e2rhioty 10 8 anatomical anatomical JJ cord-275413-e2rhioty 10 9 abnormalities abnormality NNS cord-275413-e2rhioty 10 10 were be VBD cord-275413-e2rhioty 10 11 evident evident JJ cord-275413-e2rhioty 10 12 in in IN cord-275413-e2rhioty 10 13 both both CC cord-275413-e2rhioty 10 14 infected infected JJ cord-275413-e2rhioty 10 15 and and CC cord-275413-e2rhioty 10 16 non non JJ cord-275413-e2rhioty 10 17 - - JJ cord-275413-e2rhioty 10 18 infected infected JJ cord-275413-e2rhioty 10 19 fetuses fetus NNS cord-275413-e2rhioty 10 20 from from IN cord-275413-e2rhioty 10 21 the the DT cord-275413-e2rhioty 10 22 infected infected JJ cord-275413-e2rhioty 10 23 dams dam NNS cord-275413-e2rhioty 10 24 . . . cord-275413-e2rhioty 11 1 Virus virus NN cord-275413-e2rhioty 11 2 isolation isolation NN cord-275413-e2rhioty 11 3 and and CC cord-275413-e2rhioty 11 4 immunohistochemistry immunohistochemistry NN cord-275413-e2rhioty 11 5 identified identify VBD cord-275413-e2rhioty 11 6 the the DT cord-275413-e2rhioty 11 7 thymus thymus NN cord-275413-e2rhioty 11 8 as as IN cord-275413-e2rhioty 11 9 the the DT cord-275413-e2rhioty 11 10 primary primary JJ cord-275413-e2rhioty 11 11 site site NN cord-275413-e2rhioty 11 12 of of IN cord-275413-e2rhioty 11 13 virus virus NN cord-275413-e2rhioty 11 14 replication replication NN cord-275413-e2rhioty 11 15 . . . cord-275413-e2rhioty 12 1 Steady steady JJ cord-275413-e2rhioty 12 2 state state NN cord-275413-e2rhioty 12 3 RT RT NNP cord-275413-e2rhioty 12 4 - - HYPH cord-275413-e2rhioty 12 5 PCR PCR NNP cord-275413-e2rhioty 12 6 amplification amplification NN cord-275413-e2rhioty 12 7 of of IN cord-275413-e2rhioty 12 8 inflammatory inflammatory JJ cord-275413-e2rhioty 12 9 , , , cord-275413-e2rhioty 12 10 Th1 th1 NN cord-275413-e2rhioty 12 11 and and CC cord-275413-e2rhioty 12 12 Th2 th2 CD cord-275413-e2rhioty 12 13 cytokines cytokine NNS cord-275413-e2rhioty 12 14 , , , cord-275413-e2rhioty 12 15 showed show VBD cord-275413-e2rhioty 12 16 elevated elevate VBN cord-275413-e2rhioty 12 17 IFN IFN NNP cord-275413-e2rhioty 12 18 - - HYPH cord-275413-e2rhioty 12 19 γ γ NNP cord-275413-e2rhioty 12 20 and and CC cord-275413-e2rhioty 12 21 TNF TNF NNP cord-275413-e2rhioty 12 22 - - HYPH cord-275413-e2rhioty 12 23 α α NNP cord-275413-e2rhioty 12 24 mRNAs mrna NNS cord-275413-e2rhioty 12 25 in in IN cord-275413-e2rhioty 12 26 tissues tissue NNS cord-275413-e2rhioty 12 27 from from IN cord-275413-e2rhioty 12 28 infected infected JJ cord-275413-e2rhioty 12 29 fetuses fetus NNS cord-275413-e2rhioty 12 30 , , , cord-275413-e2rhioty 12 31 which which WDT cord-275413-e2rhioty 12 32 corresponded correspond VBD cord-275413-e2rhioty 12 33 to to IN cord-275413-e2rhioty 12 34 elevated elevated JJ cord-275413-e2rhioty 12 35 cytokine cytokine NN cord-275413-e2rhioty 12 36 proteins protein NNS cord-275413-e2rhioty 12 37 in in IN cord-275413-e2rhioty 12 38 serum serum NN cord-275413-e2rhioty 12 39 but but CC cord-275413-e2rhioty 12 40 not not RB cord-275413-e2rhioty 12 41 amniotic amniotic NN cord-275413-e2rhioty 12 42 fluid fluid NN cord-275413-e2rhioty 12 43 . . . cord-275413-e2rhioty 13 1 Further further JJ cord-275413-e2rhioty 13 2 evidence evidence NN cord-275413-e2rhioty 13 3 for for IN cord-275413-e2rhioty 13 4 induction induction NN cord-275413-e2rhioty 13 5 of of IN cord-275413-e2rhioty 13 6 immunity immunity NN cord-275413-e2rhioty 13 7 was be VBD cord-275413-e2rhioty 13 8 found find VBN cord-275413-e2rhioty 13 9 in in IN cord-275413-e2rhioty 13 10 the the DT cord-275413-e2rhioty 13 11 hyperplastic hyperplastic JJ cord-275413-e2rhioty 13 12 response response NN cord-275413-e2rhioty 13 13 of of IN cord-275413-e2rhioty 13 14 lymph lymph NN cord-275413-e2rhioty 13 15 nodes node NNS cord-275413-e2rhioty 13 16 , , , cord-275413-e2rhioty 13 17 which which WDT cord-275413-e2rhioty 13 18 included include VBD cord-275413-e2rhioty 13 19 the the DT cord-275413-e2rhioty 13 20 development development NN cord-275413-e2rhioty 13 21 of of IN cord-275413-e2rhioty 13 22 germinal germinal JJ cord-275413-e2rhioty 13 23 centers center NNS cord-275413-e2rhioty 13 24 occupied occupy VBD cord-275413-e2rhioty 13 25 CDw75 CDw75 NNS cord-275413-e2rhioty 13 26 + + CC cord-275413-e2rhioty 13 27 B b NN cord-275413-e2rhioty 13 28 cells cell NNS cord-275413-e2rhioty 13 29 . . . cord-275413-e2rhioty 14 1 Collectively collectively RB cord-275413-e2rhioty 14 2 , , , cord-275413-e2rhioty 14 3 these these DT cord-275413-e2rhioty 14 4 data datum NNS cord-275413-e2rhioty 14 5 support support VBP cord-275413-e2rhioty 14 6 the the DT cord-275413-e2rhioty 14 7 notion notion NN cord-275413-e2rhioty 14 8 that that IN cord-275413-e2rhioty 14 9 the the DT cord-275413-e2rhioty 14 10 immunocompetent immunocompetent JJ cord-275413-e2rhioty 14 11 fetus fetus NN cord-275413-e2rhioty 14 12 is be VBZ cord-275413-e2rhioty 14 13 capable capable JJ cord-275413-e2rhioty 14 14 of of IN cord-275413-e2rhioty 14 15 initiating initiate VBG cord-275413-e2rhioty 14 16 an an DT cord-275413-e2rhioty 14 17 antiviral antiviral JJ cord-275413-e2rhioty 14 18 response response NN cord-275413-e2rhioty 14 19 , , , cord-275413-e2rhioty 14 20 which which WDT cord-275413-e2rhioty 14 21 is be VBZ cord-275413-e2rhioty 14 22 compartmentalized compartmentalize VBN cord-275413-e2rhioty 14 23 within within IN cord-275413-e2rhioty 14 24 the the DT cord-275413-e2rhioty 14 25 infected infected JJ cord-275413-e2rhioty 14 26 fetus fetus NN cord-275413-e2rhioty 14 27 . . . cord-275413-e2rhioty 15 1 Furthermore furthermore RB cord-275413-e2rhioty 15 2 , , , cord-275413-e2rhioty 15 3 fetal fetal JJ cord-275413-e2rhioty 15 4 pathology pathology NN cord-275413-e2rhioty 15 5 may may MD cord-275413-e2rhioty 15 6 not not RB cord-275413-e2rhioty 15 7 be be VB cord-275413-e2rhioty 15 8 a a DT cord-275413-e2rhioty 15 9 direct direct JJ cord-275413-e2rhioty 15 10 result result NN cord-275413-e2rhioty 15 11 of of IN cord-275413-e2rhioty 15 12 virus virus NN cord-275413-e2rhioty 15 13 replication replication NN cord-275413-e2rhioty 15 14 in in IN cord-275413-e2rhioty 15 15 the the DT cord-275413-e2rhioty 15 16 fetus fetus NN cord-275413-e2rhioty 15 17 . . . cord-275413-e2rhioty 16 1 Porcine porcine JJ cord-275413-e2rhioty 16 2 reproductive reproductive JJ cord-275413-e2rhioty 16 3 and and CC cord-275413-e2rhioty 16 4 respiratory respiratory JJ cord-275413-e2rhioty 16 5 syndrome syndrome NN cord-275413-e2rhioty 16 6 ( ( -LRB- cord-275413-e2rhioty 16 7 PRRS PRRS NNP cord-275413-e2rhioty 16 8 ) ) -RRB- cord-275413-e2rhioty 16 9 is be VBZ cord-275413-e2rhioty 16 10 caused cause VBN cord-275413-e2rhioty 16 11 by by IN cord-275413-e2rhioty 16 12 an an DT cord-275413-e2rhioty 16 13 enveloped envelop VBN cord-275413-e2rhioty 16 14 positive positive JJ cord-275413-e2rhioty 16 15 - - HYPH cord-275413-e2rhioty 16 16 stranded strand VBN cord-275413-e2rhioty 16 17 RNA RNA NNP cord-275413-e2rhioty 16 18 virus virus NN cord-275413-e2rhioty 16 19 , , , cord-275413-e2rhioty 16 20 PRRSV PRRSV NNP cord-275413-e2rhioty 16 21 , , , cord-275413-e2rhioty 16 22 belonging belong VBG cord-275413-e2rhioty 16 23 to to IN cord-275413-e2rhioty 16 24 the the DT cord-275413-e2rhioty 16 25 family family NN cord-275413-e2rhioty 16 26 Arteriviridae Arteriviridae NNP cord-275413-e2rhioty 16 27 Cavanagh Cavanagh NNP cord-275413-e2rhioty 16 28 , , , cord-275413-e2rhioty 16 29 1997 1997 CD cord-275413-e2rhioty 16 30 ; ; : cord-275413-e2rhioty 16 31 Nelsen Nelsen NNP cord-275413-e2rhioty 16 32 et et NNP cord-275413-e2rhioty 16 33 al al NNP cord-275413-e2rhioty 16 34 . . NNP cord-275413-e2rhioty 16 35 , , , cord-275413-e2rhioty 16 36 1999 1999 CD cord-275413-e2rhioty 16 37 ; ; : cord-275413-e2rhioty 16 38 Wensvoort Wensvoort NNP cord-275413-e2rhioty 16 39 et et NNP cord-275413-e2rhioty 16 40 al al NNP cord-275413-e2rhioty 16 41 . . NNP cord-275413-e2rhioty 16 42 , , , cord-275413-e2rhioty 16 43 1991 1991 CD cord-275413-e2rhioty 16 44 ) ) -RRB- cord-275413-e2rhioty 16 45 . . . cord-275413-e2rhioty 17 1 Other other JJ cord-275413-e2rhioty 17 2 members member NNS cord-275413-e2rhioty 17 3 of of IN cord-275413-e2rhioty 17 4 the the DT cord-275413-e2rhioty 17 5 arterivirus arterivirus NNP cord-275413-e2rhioty 17 6 group group NN cord-275413-e2rhioty 17 7 include include VBP cord-275413-e2rhioty 17 8 lactate lactate NN cord-275413-e2rhioty 17 9 dehydrogenase dehydrogenase NN cord-275413-e2rhioty 17 10 - - HYPH cord-275413-e2rhioty 17 11 elevating elevate VBG cord-275413-e2rhioty 17 12 virus virus NN cord-275413-e2rhioty 17 13 ( ( -LRB- cord-275413-e2rhioty 17 14 LDV LDV NNP cord-275413-e2rhioty 17 15 ) ) -RRB- cord-275413-e2rhioty 17 16 of of IN cord-275413-e2rhioty 17 17 mice mouse NNS cord-275413-e2rhioty 17 18 , , , cord-275413-e2rhioty 17 19 equine equine JJ cord-275413-e2rhioty 17 20 arteritis arteritis NN cord-275413-e2rhioty 17 21 virus virus NN cord-275413-e2rhioty 17 22 ( ( -LRB- cord-275413-e2rhioty 17 23 EAV EAV NNP cord-275413-e2rhioty 17 24 ) ) -RRB- cord-275413-e2rhioty 17 25 , , , cord-275413-e2rhioty 17 26 and and CC cord-275413-e2rhioty 17 27 simian simian JJ cord-275413-e2rhioty 17 28 hemorrhagic hemorrhagic JJ cord-275413-e2rhioty 17 29 fever fever NN cord-275413-e2rhioty 17 30 virus virus NN cord-275413-e2rhioty 17 31 ( ( -LRB- cord-275413-e2rhioty 17 32 SHFV SHFV NNP cord-275413-e2rhioty 17 33 ; ; : cord-275413-e2rhioty 17 34 for for IN cord-275413-e2rhioty 17 35 review review NN cord-275413-e2rhioty 17 36 see see VBP cord-275413-e2rhioty 17 37 Plagemann Plagemann NNP cord-275413-e2rhioty 17 38 , , , cord-275413-e2rhioty 17 39 1996 1996 CD cord-275413-e2rhioty 17 40 ) ) -RRB- cord-275413-e2rhioty 17 41 . . . cord-275413-e2rhioty 18 1 The the DT cord-275413-e2rhioty 18 2 arteriviruses arteriviruse NNS cord-275413-e2rhioty 18 3 , , , cord-275413-e2rhioty 18 4 toroviruses toroviruses NNP cord-275413-e2rhioty 18 5 , , , cord-275413-e2rhioty 18 6 roniviruses roniviruses NNP cord-275413-e2rhioty 18 7 and and CC cord-275413-e2rhioty 18 8 coronaviruses coronaviruse NNS cord-275413-e2rhioty 18 9 form form VBP cord-275413-e2rhioty 18 10 a a DT cord-275413-e2rhioty 18 11 single single JJ cord-275413-e2rhioty 18 12 order order NN cord-275413-e2rhioty 18 13 , , , cord-275413-e2rhioty 18 14 Nidovirales Nidovirales NNP cord-275413-e2rhioty 18 15 . . . cord-275413-e2rhioty 19 1 Arteriviruses Arteriviruses NNP cord-275413-e2rhioty 19 2 structurally structurally RB cord-275413-e2rhioty 19 3 resemble resemble VBP cord-275413-e2rhioty 19 4 togaviruses togaviruse NNS cord-275413-e2rhioty 19 5 , , , cord-275413-e2rhioty 19 6 but but CC cord-275413-e2rhioty 19 7 similar similar JJ cord-275413-e2rhioty 19 8 to to IN cord-275413-e2rhioty 19 9 coronaviruses coronaviruse NNS cord-275413-e2rhioty 19 10 , , , cord-275413-e2rhioty 19 11 replicate replicate VB cord-275413-e2rhioty 19 12 via via IN cord-275413-e2rhioty 19 13 a a DT cord-275413-e2rhioty 19 14 nested nested JJ cord-275413-e2rhioty 19 15 3 3 CD cord-275413-e2rhioty 19 16 -co -co NN cord-275413-e2rhioty 19 17 - - HYPH cord-275413-e2rhioty 19 18 terminal terminal NN cord-275413-e2rhioty 19 19 set set NN cord-275413-e2rhioty 19 20 of of IN cord-275413-e2rhioty 19 21 subgenomic subgenomic NNP cord-275413-e2rhioty 19 22 mRNAs mRNAs NNPS cord-275413-e2rhioty 19 23 that that WDT cord-275413-e2rhioty 19 24 possesses possess VBZ cord-275413-e2rhioty 19 25 a a DT cord-275413-e2rhioty 19 26 common common JJ cord-275413-e2rhioty 19 27 leader leader NN cord-275413-e2rhioty 19 28 and and CC cord-275413-e2rhioty 19 29 a a DT cord-275413-e2rhioty 19 30 poly poly JJ cord-275413-e2rhioty 19 31 - - HYPH cord-275413-e2rhioty 19 32 A a DT cord-275413-e2rhioty 19 33 tail tail NN cord-275413-e2rhioty 19 34 ( ( -LRB- cord-275413-e2rhioty 19 35 reviewed review VBN cord-275413-e2rhioty 19 36 in in IN cord-275413-e2rhioty 19 37 Snijder Snijder NNP cord-275413-e2rhioty 19 38 and and CC cord-275413-e2rhioty 19 39 Mulenberg Mulenberg NNP cord-275413-e2rhioty 19 40 , , , cord-275413-e2rhioty 19 41 1998 1998 CD cord-275413-e2rhioty 19 42 ) ) -RRB- cord-275413-e2rhioty 19 43 . . . cord-275413-e2rhioty 20 1 The the DT cord-275413-e2rhioty 20 2 arteriviruses arteriviruse NNS cord-275413-e2rhioty 20 3 exhibit exhibit VBP cord-275413-e2rhioty 20 4 several several JJ cord-275413-e2rhioty 20 5 important important JJ cord-275413-e2rhioty 20 6 properties property NNS cord-275413-e2rhioty 20 7 relevant relevant JJ cord-275413-e2rhioty 20 8 to to IN cord-275413-e2rhioty 20 9 the the DT cord-275413-e2rhioty 20 10 study study NN cord-275413-e2rhioty 20 11 of of IN cord-275413-e2rhioty 20 12 viral viral JJ cord-275413-e2rhioty 20 13 pathogenesis pathogenesis NN cord-275413-e2rhioty 20 14 , , , cord-275413-e2rhioty 20 15 including include VBG cord-275413-e2rhioty 20 16 cytopathic cytopathic JJ cord-275413-e2rhioty 20 17 replication replication NN cord-275413-e2rhioty 20 18 in in IN cord-275413-e2rhioty 20 19 macrophages macrophage NNS cord-275413-e2rhioty 20 20 , , , cord-275413-e2rhioty 20 21 the the DT cord-275413-e2rhioty 20 22 capacity capacity NN cord-275413-e2rhioty 20 23 to to TO cord-275413-e2rhioty 20 24 establish establish VB cord-275413-e2rhioty 20 25 and and CC cord-275413-e2rhioty 20 26 maintain maintain VB cord-275413-e2rhioty 20 27 an an DT cord-275413-e2rhioty 20 28 asymptomatic asymptomatic JJ cord-275413-e2rhioty 20 29 infec infec NN cord-275413-e2rhioty 20 30 - - HYPH cord-275413-e2rhioty 20 31 tion tion NN cord-275413-e2rhioty 20 32 , , , cord-275413-e2rhioty 20 33 as as RB cord-275413-e2rhioty 20 34 well well RB cord-275413-e2rhioty 20 35 as as IN cord-275413-e2rhioty 20 36 cause cause NN cord-275413-e2rhioty 20 37 severe severe JJ cord-275413-e2rhioty 20 38 and and CC cord-275413-e2rhioty 20 39 fatal fatal JJ cord-275413-e2rhioty 20 40 disease disease NN cord-275413-e2rhioty 20 41 ( ( -LRB- cord-275413-e2rhioty 20 42 Plagemann Plagemann NNP cord-275413-e2rhioty 20 43 , , , cord-275413-e2rhioty 20 44 1996 1996 CD cord-275413-e2rhioty 20 45 ) ) -RRB- cord-275413-e2rhioty 20 46 . . . cord-275413-e2rhioty 21 1 Infection infection NN cord-275413-e2rhioty 21 2 of of IN cord-275413-e2rhioty 21 3 adult adult NN cord-275413-e2rhioty 21 4 pigs pig NNS cord-275413-e2rhioty 21 5 with with IN cord-275413-e2rhioty 21 6 PRRSV PRRSV NNP cord-275413-e2rhioty 21 7 usually usually RB cord-275413-e2rhioty 21 8 produces produce VBZ cord-275413-e2rhioty 21 9 a a DT cord-275413-e2rhioty 21 10 non non JJ cord-275413-e2rhioty 21 11 - - JJ cord-275413-e2rhioty 21 12 fatal fatal JJ cord-275413-e2rhioty 21 13 disease disease NN cord-275413-e2rhioty 21 14 , , , cord-275413-e2rhioty 21 15 characterized characterize VBN cord-275413-e2rhioty 21 16 by by IN cord-275413-e2rhioty 21 17 flu flu NN cord-275413-e2rhioty 21 18 - - HYPH cord-275413-e2rhioty 21 19 like like JJ cord-275413-e2rhioty 21 20 symptoms symptom NNS cord-275413-e2rhioty 21 21 , , , cord-275413-e2rhioty 21 22 a a DT cord-275413-e2rhioty 21 23 transient transient JJ cord-275413-e2rhioty 21 24 elevation elevation NN cord-275413-e2rhioty 21 25 in in IN cord-275413-e2rhioty 21 26 temperature temperature NN cord-275413-e2rhioty 21 27 and and CC cord-275413-e2rhioty 21 28 inappetance inappetance NN cord-275413-e2rhioty 21 29 ( ( -LRB- cord-275413-e2rhioty 21 30 reviewed review VBN cord-275413-e2rhioty 21 31 in in IN cord-275413-e2rhioty 21 32 Benfield Benfield NNP cord-275413-e2rhioty 21 33 et et NNP cord-275413-e2rhioty 21 34 al al NNP cord-275413-e2rhioty 21 35 . . NNP cord-275413-e2rhioty 21 36 , , , cord-275413-e2rhioty 21 37 1999 1999 CD cord-275413-e2rhioty 21 38 ; ; : cord-275413-e2rhioty 21 39 Christianson Christianson NNP cord-275413-e2rhioty 21 40 et et NNP cord-275413-e2rhioty 21 41 al al NNP cord-275413-e2rhioty 21 42 . . NNP cord-275413-e2rhioty 21 43 , , , cord-275413-e2rhioty 21 44 1992 1992 CD cord-275413-e2rhioty 21 45 ) ) -RRB- cord-275413-e2rhioty 21 46 . . . cord-275413-e2rhioty 22 1 The the DT cord-275413-e2rhioty 22 2 reproductive reproductive JJ cord-275413-e2rhioty 22 3 form form NN cord-275413-e2rhioty 22 4 of of IN cord-275413-e2rhioty 22 5 PRRS PRRS NNP cord-275413-e2rhioty 22 6 occurs occur VBZ cord-275413-e2rhioty 22 7 following follow VBG cord-275413-e2rhioty 22 8 the the DT cord-275413-e2rhioty 22 9 infection infection NN cord-275413-e2rhioty 22 10 of of IN cord-275413-e2rhioty 22 11 late late JJ cord-275413-e2rhioty 22 12 gestation gestation NN cord-275413-e2rhioty 22 13 pregnant pregnant JJ cord-275413-e2rhioty 22 14 gilts gilt NNS cord-275413-e2rhioty 22 15 or or CC cord-275413-e2rhioty 22 16 sows sow NNS cord-275413-e2rhioty 22 17 . . . cord-275413-e2rhioty 23 1 Natural natural JJ cord-275413-e2rhioty 23 2 infection infection NN cord-275413-e2rhioty 23 3 of of IN cord-275413-e2rhioty 23 4 the the DT cord-275413-e2rhioty 23 5 fetus fetus NN cord-275413-e2rhioty 23 6 with with IN cord-275413-e2rhioty 23 7 PRRSV PRRSV NNP cord-275413-e2rhioty 23 8 is be VBZ cord-275413-e2rhioty 23 9 initiated initiate VBN cord-275413-e2rhioty 23 10 with with IN cord-275413-e2rhioty 23 11 the the DT cord-275413-e2rhioty 23 12 infection infection NN cord-275413-e2rhioty 23 13 of of IN cord-275413-e2rhioty 23 14 gilts gilt NNS cord-275413-e2rhioty 23 15 and and CC cord-275413-e2rhioty 23 16 sows sow NNS cord-275413-e2rhioty 23 17 at at IN cord-275413-e2rhioty 23 18 90 90 CD cord-275413-e2rhioty 23 19 days day NNS cord-275413-e2rhioty 23 20 gestation gestation NN cord-275413-e2rhioty 23 21 . . . cord-275413-e2rhioty 24 1 After after IN cord-275413-e2rhioty 24 2 productive productive JJ cord-275413-e2rhioty 24 3 replication replication NN cord-275413-e2rhioty 24 4 on on IN cord-275413-e2rhioty 24 5 the the DT cord-275413-e2rhioty 24 6 maternal maternal JJ cord-275413-e2rhioty 24 7 side side NN cord-275413-e2rhioty 24 8 , , , cord-275413-e2rhioty 24 9 the the DT cord-275413-e2rhioty 24 10 virus virus NN cord-275413-e2rhioty 24 11 crosses cross VBZ cord-275413-e2rhioty 24 12 the the DT cord-275413-e2rhioty 24 13 placenta placenta NN cord-275413-e2rhioty 24 14 and and CC cord-275413-e2rhioty 24 15 productively productively RB cord-275413-e2rhioty 24 16 infects infect VBZ cord-275413-e2rhioty 24 17 the the DT cord-275413-e2rhioty 24 18 fetus fetus NN cord-275413-e2rhioty 24 19 . . . cord-275413-e2rhioty 25 1 The the DT cord-275413-e2rhioty 25 2 mechanism mechanism NN cord-275413-e2rhioty 25 3 of of IN cord-275413-e2rhioty 25 4 transplacental transplacental NNP cord-275413-e2rhioty 25 5 infection infection NN cord-275413-e2rhioty 25 6 is be VBZ cord-275413-e2rhioty 25 7 unknown unknown JJ cord-275413-e2rhioty 25 8 , , , cord-275413-e2rhioty 25 9 but but CC cord-275413-e2rhioty 25 10 could could MD cord-275413-e2rhioty 25 11 be be VB cord-275413-e2rhioty 25 12 similar similar JJ cord-275413-e2rhioty 25 13 to to IN cord-275413-e2rhioty 25 14 the the DT cord-275413-e2rhioty 25 15 infected infected JJ cord-275413-e2rhioty 25 16 " " `` cord-275413-e2rhioty 25 17 Trojan Trojan NNP cord-275413-e2rhioty 25 18 Horse Horse NNP cord-275413-e2rhioty 25 19 " " '' cord-275413-e2rhioty 25 20 macrophage macrophage NN cord-275413-e2rhioty 25 21 , , , cord-275413-e2rhioty 25 22 described describe VBN cord-275413-e2rhioty 25 23 for for IN cord-275413-e2rhioty 25 24 LDV LDV NNP cord-275413-e2rhioty 25 25 ( ( -LRB- cord-275413-e2rhioty 25 26 Cafruny Cafruny NNP cord-275413-e2rhioty 25 27 and and CC cord-275413-e2rhioty 25 28 Bradley Bradley NNP cord-275413-e2rhioty 25 29 , , , cord-275413-e2rhioty 25 30 1996 1996 CD cord-275413-e2rhioty 25 31 ) ) -RRB- cord-275413-e2rhioty 25 32 . . . cord-275413-e2rhioty 26 1 Since since IN cord-275413-e2rhioty 26 2 the the DT cord-275413-e2rhioty 26 3 pig pig NN cord-275413-e2rhioty 26 4 fetus fetus NN cord-275413-e2rhioty 26 5 becomes become VBZ cord-275413-e2rhioty 26 6 immunocompetent immunocompetent JJ cord-275413-e2rhioty 26 7 at at IN cord-275413-e2rhioty 26 8 about about RB cord-275413-e2rhioty 26 9 70 70 CD cord-275413-e2rhioty 26 10 days day NNS cord-275413-e2rhioty 26 11 of of IN cord-275413-e2rhioty 26 12 gestation gestation NN cord-275413-e2rhioty 26 13 , , , cord-275413-e2rhioty 26 14 PRRSV PRRSV NNP cord-275413-e2rhioty 26 15 infection infection NN cord-275413-e2rhioty 26 16 occurs occur VBZ cord-275413-e2rhioty 26 17 in in IN cord-275413-e2rhioty 26 18 an an DT cord-275413-e2rhioty 26 19 immune immune JJ cord-275413-e2rhioty 26 20 environment environment NN cord-275413-e2rhioty 26 21 containing contain VBG cord-275413-e2rhioty 26 22 functional functional JJ cord-275413-e2rhioty 26 23 B b NN cord-275413-e2rhioty 26 24 and and CC cord-275413-e2rhioty 26 25 T t NN cord-275413-e2rhioty 26 26 cells cell NNS cord-275413-e2rhioty 26 27 . . . cord-275413-e2rhioty 27 1 Accordingly accordingly RB cord-275413-e2rhioty 27 2 , , , cord-275413-e2rhioty 27 3 virus virus NN cord-275413-e2rhioty 27 4 - - HYPH cord-275413-e2rhioty 27 5 induced induce VBN cord-275413-e2rhioty 27 6 reproductive reproductive JJ cord-275413-e2rhioty 27 7 failure failure NN cord-275413-e2rhioty 27 8 can can MD cord-275413-e2rhioty 27 9 present present VB cord-275413-e2rhioty 27 10 clinically clinically RB cord-275413-e2rhioty 27 11 as as IN cord-275413-e2rhioty 27 12 delayed delay VBN cord-275413-e2rhioty 27 13 returns return NNS cord-275413-e2rhioty 27 14 to to IN cord-275413-e2rhioty 27 15 estrus estrus NN cord-275413-e2rhioty 27 16 , , , cord-275413-e2rhioty 27 17 as as RB cord-275413-e2rhioty 27 18 well well RB cord-275413-e2rhioty 27 19 as as IN cord-275413-e2rhioty 27 20 abortions abortion NNS cord-275413-e2rhioty 27 21 , , , cord-275413-e2rhioty 27 22 mummified mummified JJ cord-275413-e2rhioty 27 23 fetuses fetus NNS cord-275413-e2rhioty 27 24 , , , cord-275413-e2rhioty 27 25 stillborn stillborn JJ cord-275413-e2rhioty 27 26 and and CC cord-275413-e2rhioty 27 27 weak weak JJ cord-275413-e2rhioty 27 28 - - HYPH cord-275413-e2rhioty 27 29 born bear VBN cord-275413-e2rhioty 27 30 pigs pig NNS cord-275413-e2rhioty 27 31 Christianson Christianson NNP cord-275413-e2rhioty 27 32 et et NNP cord-275413-e2rhioty 27 33 al al NNP cord-275413-e2rhioty 27 34 . . NNP cord-275413-e2rhioty 27 35 , , , cord-275413-e2rhioty 27 36 1993 1993 CD cord-275413-e2rhioty 27 37 ; ; : cord-275413-e2rhioty 27 38 Collins Collins NNP cord-275413-e2rhioty 27 39 et et NNP cord-275413-e2rhioty 27 40 al al NNP cord-275413-e2rhioty 27 41 . . NNP cord-275413-e2rhioty 27 42 , , , cord-275413-e2rhioty 27 43 1992 1992 CD cord-275413-e2rhioty 27 44 ; ; : cord-275413-e2rhioty 27 45 Mengeling Mengeling NNP cord-275413-e2rhioty 27 46 et et FW cord-275413-e2rhioty 27 47 al al NNP cord-275413-e2rhioty 27 48 . . NNP cord-275413-e2rhioty 27 49 , , , cord-275413-e2rhioty 27 50 1994 1994 CD cord-275413-e2rhioty 27 51 ; ; . cord-275413-e2rhioty 28 1 Rossow Rossow NNP cord-275413-e2rhioty 28 2 et et NNP cord-275413-e2rhioty 28 3 al al NNP cord-275413-e2rhioty 28 4 . . NNP cord-275413-e2rhioty 28 5 , , , cord-275413-e2rhioty 28 6 1999 1999 CD cord-275413-e2rhioty 28 7 ; ; : cord-275413-e2rhioty 28 8 Rowland Rowland NNP cord-275413-e2rhioty 28 9 et et NNP cord-275413-e2rhioty 28 10 al al NNP cord-275413-e2rhioty 28 11 . . NNP cord-275413-e2rhioty 28 12 , , , cord-275413-e2rhioty 28 13 2003 2003 CD cord-275413-e2rhioty 28 14 ) ) -RRB- cord-275413-e2rhioty 28 15 . . . cord-275413-e2rhioty 29 1 Surviving survive VBG cord-275413-e2rhioty 29 2 neonates neonate NNS cord-275413-e2rhioty 29 3 can can MD cord-275413-e2rhioty 29 4 exhibit exhibit VB cord-275413-e2rhioty 29 5 the the DT cord-275413-e2rhioty 29 6 severest severe JJS cord-275413-e2rhioty 29 7 form form NN cord-275413-e2rhioty 29 8 of of IN cord-275413-e2rhioty 29 9 respiratory respiratory JJ cord-275413-e2rhioty 29 10 disease disease NN cord-275413-e2rhioty 29 11 with with IN cord-275413-e2rhioty 29 12 mortality mortality NN cord-275413-e2rhioty 29 13 sometimes sometimes RB cord-275413-e2rhioty 29 14 reaching reach VBG cord-275413-e2rhioty 29 15 100 100 CD cord-275413-e2rhioty 29 16 % % NN cord-275413-e2rhioty 29 17 within within IN cord-275413-e2rhioty 29 18 three three CD cord-275413-e2rhioty 29 19 weeks week NNS cord-275413-e2rhioty 29 20 after after IN cord-275413-e2rhioty 29 21 birth birth NN cord-275413-e2rhioty 30 1 ( ( -LRB- cord-275413-e2rhioty 30 2 Feng Feng NNP cord-275413-e2rhioty 30 3 et et FW cord-275413-e2rhioty 30 4 al al NNP cord-275413-e2rhioty 30 5 . . NNP cord-275413-e2rhioty 30 6 , , , cord-275413-e2rhioty 30 7 2001 2001 CD cord-275413-e2rhioty 30 8 ; ; : cord-275413-e2rhioty 31 1 Rossow Rossow NNP cord-275413-e2rhioty 31 2 et et NNP cord-275413-e2rhioty 31 3 al al NNP cord-275413-e2rhioty 31 4 . . . cord-275413-e2rhioty 32 1 , , , cord-275413-e2rhioty 32 2 1994 1994 CD cord-275413-e2rhioty 32 3 ; ; : cord-275413-e2rhioty 32 4 Rossow Rossow NNP cord-275413-e2rhioty 32 5 , , , cord-275413-e2rhioty 32 6 1998 1998 CD cord-275413-e2rhioty 32 7 ) ) -RRB- cord-275413-e2rhioty 32 8 . . . cord-275413-e2rhioty 33 1 The the DT cord-275413-e2rhioty 33 2 complex complex JJ cord-275413-e2rhioty 33 3 pathology pathology NN cord-275413-e2rhioty 33 4 following follow VBG cord-275413-e2rhioty 33 5 exposure exposure NN cord-275413-e2rhioty 33 6 to to IN cord-275413-e2rhioty 33 7 PRRSV PRRSV NNP cord-275413-e2rhioty 33 8 in in IN cord-275413-e2rhioty 33 9 utero utero NN cord-275413-e2rhioty 33 10 represents represent VBZ cord-275413-e2rhioty 33 11 a a DT cord-275413-e2rhioty 33 12 unique unique JJ cord-275413-e2rhioty 33 13 form form NN cord-275413-e2rhioty 33 14 of of IN cord-275413-e2rhioty 33 15 the the DT cord-275413-e2rhioty 33 16 disease disease NN cord-275413-e2rhioty 33 17 referred refer VBD cord-275413-e2rhioty 33 18 to to IN cord-275413-e2rhioty 33 19 as as IN cord-275413-e2rhioty 33 20 congenital congenital JJ cord-275413-e2rhioty 33 21 PRRS PRRS NNP cord-275413-e2rhioty 34 1 ( ( -LRB- cord-275413-e2rhioty 34 2 Rowland Rowland NNP cord-275413-e2rhioty 34 3 et et NNP cord-275413-e2rhioty 34 4 al al NNP cord-275413-e2rhioty 34 5 . . NNP cord-275413-e2rhioty 34 6 , , , cord-275413-e2rhioty 34 7 2003 2003 CD cord-275413-e2rhioty 34 8 ) ) -RRB- cord-275413-e2rhioty 34 9 . . . cord-275413-e2rhioty 35 1 The the DT cord-275413-e2rhioty 35 2 purpose purpose NN cord-275413-e2rhioty 35 3 of of IN cord-275413-e2rhioty 35 4 this this DT cord-275413-e2rhioty 35 5 study study NN cord-275413-e2rhioty 35 6 was be VBD cord-275413-e2rhioty 35 7 to to TO cord-275413-e2rhioty 35 8 characterize characterize VB cord-275413-e2rhioty 35 9 the the DT cord-275413-e2rhioty 35 10 interaction interaction NN cord-275413-e2rhioty 35 11 between between IN cord-275413-e2rhioty 35 12 PRRSV PRRSV NNP cord-275413-e2rhioty 35 13 and and CC cord-275413-e2rhioty 35 14 the the DT cord-275413-e2rhioty 35 15 pig pig NN cord-275413-e2rhioty 35 16 fetus fetus NN cord-275413-e2rhioty 35 17 by by IN cord-275413-e2rhioty 35 18 ( ( -LRB- cord-275413-e2rhioty 35 19 1 1 CD cord-275413-e2rhioty 35 20 ) ) -RRB- cord-275413-e2rhioty 35 21 identifying identify VBG cord-275413-e2rhioty 35 22 sites site NNS cord-275413-e2rhioty 35 23 of of IN cord-275413-e2rhioty 35 24 virus virus NN cord-275413-e2rhioty 35 25 replication replication NN cord-275413-e2rhioty 35 26 , , , cord-275413-e2rhioty 35 27 ( ( -LRB- cord-275413-e2rhioty 35 28 2 2 LS cord-275413-e2rhioty 35 29 ) ) -RRB- cord-275413-e2rhioty 35 30 measuring measure VBG cord-275413-e2rhioty 35 31 immune immune JJ cord-275413-e2rhioty 35 32 and and CC cord-275413-e2rhioty 35 33 inflammatory inflammatory JJ cord-275413-e2rhioty 35 34 cytokines cytokine NNS cord-275413-e2rhioty 35 35 in in IN cord-275413-e2rhioty 35 36 different different JJ cord-275413-e2rhioty 35 37 compartments compartment NNS cord-275413-e2rhioty 35 38 , , , cord-275413-e2rhioty 35 39 and and CC cord-275413-e2rhioty 35 40 ( ( -LRB- cord-275413-e2rhioty 35 41 3 3 LS cord-275413-e2rhioty 35 42 ) ) -RRB- cord-275413-e2rhioty 35 43 evaluating evaluate VBG cord-275413-e2rhioty 35 44 the the DT cord-275413-e2rhioty 35 45 response response NN cord-275413-e2rhioty 35 46 of of IN cord-275413-e2rhioty 35 47 lymph lymph NN cord-275413-e2rhioty 35 48 nodes node NNS cord-275413-e2rhioty 35 49 . . . cord-275413-e2rhioty 36 1 Experiments experiment NNS cord-275413-e2rhioty 36 2 involving involve VBG cord-275413-e2rhioty 36 3 animals animal NNS cord-275413-e2rhioty 36 4 were be VBD cord-275413-e2rhioty 36 5 approved approve VBN cord-275413-e2rhioty 36 6 by by IN cord-275413-e2rhioty 36 7 the the DT cord-275413-e2rhioty 36 8 Kansas Kansas NNP cord-275413-e2rhioty 36 9 State State NNP cord-275413-e2rhioty 36 10 University University NNP cord-275413-e2rhioty 36 11 IACU IACU NNP cord-275413-e2rhioty 36 12 Committee Committee NNP cord-275413-e2rhioty 36 13 . . . cord-275413-e2rhioty 37 1 Pregnant pregnant JJ cord-275413-e2rhioty 37 2 sows sow NNS cord-275413-e2rhioty 37 3 , , , cord-275413-e2rhioty 37 4 obtained obtain VBN cord-275413-e2rhioty 37 5 from from IN cord-275413-e2rhioty 37 6 a a DT cord-275413-e2rhioty 37 7 closely closely RB cord-275413-e2rhioty 37 8 monitored monitor VBN cord-275413-e2rhioty 37 9 PRRSV PRRSV NNP cord-275413-e2rhioty 37 10 - - HYPH cord-275413-e2rhioty 37 11 negative negative JJ cord-275413-e2rhioty 37 12 herd herd NN cord-275413-e2rhioty 37 13 , , , cord-275413-e2rhioty 37 14 were be VBD cord-275413-e2rhioty 37 15 challenged challenge VBN cord-275413-e2rhioty 37 16 at at IN cord-275413-e2rhioty 37 17 90 90 CD cord-275413-e2rhioty 37 18 days day NNS cord-275413-e2rhioty 37 19 gestation gestation NN cord-275413-e2rhioty 37 20 with with IN cord-275413-e2rhioty 37 21 a a DT cord-275413-e2rhioty 37 22 sixth sixth JJ cord-275413-e2rhioty 37 23 passage passage NN cord-275413-e2rhioty 37 24 isolate isolate NN cord-275413-e2rhioty 37 25 of of IN cord-275413-e2rhioty 37 26 SD-23983 SD-23983 NNS cord-275413-e2rhioty 37 27 , , , cord-275413-e2rhioty 37 28 a a DT cord-275413-e2rhioty 37 29 typical typical JJ cord-275413-e2rhioty 37 30 North north JJ cord-275413-e2rhioty 37 31 American american JJ cord-275413-e2rhioty 37 32 field field NN cord-275413-e2rhioty 37 33 isolate isolate VBP cord-275413-e2rhioty 37 34 ( ( -LRB- cord-275413-e2rhioty 37 35 Rowland Rowland NNP cord-275413-e2rhioty 37 36 et et NNP cord-275413-e2rhioty 37 37 al al NNP cord-275413-e2rhioty 37 38 . . NNP cord-275413-e2rhioty 37 39 , , , cord-275413-e2rhioty 37 40 2001 2001 CD cord-275413-e2rhioty 37 41 ) ) -RRB- cord-275413-e2rhioty 37 42 . . . cord-275413-e2rhioty 38 1 The the DT cord-275413-e2rhioty 38 2 methods method NNS cord-275413-e2rhioty 38 3 for for IN cord-275413-e2rhioty 38 4 the the DT cord-275413-e2rhioty 38 5 preparation preparation NN cord-275413-e2rhioty 38 6 of of IN cord-275413-e2rhioty 38 7 the the DT cord-275413-e2rhioty 38 8 PRRSV PRRSV NNP cord-275413-e2rhioty 38 9 inoculum inoculum NN cord-275413-e2rhioty 38 10 on on IN cord-275413-e2rhioty 38 11 MARC-145 MARC-145 NNP cord-275413-e2rhioty 38 12 cells cell NNS cord-275413-e2rhioty 38 13 and and CC cord-275413-e2rhioty 38 14 infection infection NN cord-275413-e2rhioty 38 15 of of IN cord-275413-e2rhioty 38 16 pigs pig NNS cord-275413-e2rhioty 38 17 are be VBP cord-275413-e2rhioty 38 18 described describe VBN cord-275413-e2rhioty 38 19 in in IN cord-275413-e2rhioty 38 20 Rowland Rowland NNP cord-275413-e2rhioty 38 21 et et NNP cord-275413-e2rhioty 38 22 al al NNP cord-275413-e2rhioty 38 23 . . . cord-275413-e2rhioty 39 1 ( ( -LRB- cord-275413-e2rhioty 39 2 2003 2003 CD cord-275413-e2rhioty 39 3 ) ) -RRB- cord-275413-e2rhioty 39 4 . . . cord-275413-e2rhioty 40 1 Virus virus NN cord-275413-e2rhioty 40 2 was be VBD cord-275413-e2rhioty 40 3 cultivated cultivate VBN cord-275413-e2rhioty 40 4 on on IN cord-275413-e2rhioty 40 5 MARC-145 MARC-145 NNP cord-275413-e2rhioty 40 6 cells cell NNS cord-275413-e2rhioty 40 7 in in IN cord-275413-e2rhioty 40 8 MEM mem NN cord-275413-e2rhioty 40 9 supplemented supplement VBN cord-275413-e2rhioty 40 10 with with IN cord-275413-e2rhioty 40 11 antibiotics antibiotic NNS cord-275413-e2rhioty 40 12 ( ( -LRB- cord-275413-e2rhioty 40 13 pen pen NN cord-275413-e2rhioty 40 14 / / SYM cord-275413-e2rhioty 40 15 step step NN cord-275413-e2rhioty 40 16 ) ) -RRB- cord-275413-e2rhioty 40 17 and and CC cord-275413-e2rhioty 40 18 7 7 CD cord-275413-e2rhioty 40 19 % % NN cord-275413-e2rhioty 40 20 FBS FBS NNP cord-275413-e2rhioty 40 21 . . . cord-275413-e2rhioty 41 1 Dams dam NNS cord-275413-e2rhioty 41 2 , , , cord-275413-e2rhioty 41 3 at at IN cord-275413-e2rhioty 41 4 90 90 CD cord-275413-e2rhioty 41 5 days day NNS cord-275413-e2rhioty 41 6 gestation gestation NN cord-275413-e2rhioty 41 7 were be VBD cord-275413-e2rhioty 41 8 challenged challenge VBN cord-275413-e2rhioty 41 9 with with IN cord-275413-e2rhioty 41 10 approximately approximately RB cord-275413-e2rhioty 41 11 10 10 CD cord-275413-e2rhioty 41 12 5 5 CD cord-275413-e2rhioty 41 13 TCID TCID NNP cord-275413-e2rhioty 41 14 50 50 CD cord-275413-e2rhioty 41 15 of of IN cord-275413-e2rhioty 41 16 virus virus NN cord-275413-e2rhioty 41 17 diluted dilute VBN cord-275413-e2rhioty 41 18 in in IN cord-275413-e2rhioty 41 19 5 5 CD cord-275413-e2rhioty 41 20 ml ml NNS cord-275413-e2rhioty 41 21 of of IN cord-275413-e2rhioty 41 22 culture culture NN cord-275413-e2rhioty 41 23 medium medium NN cord-275413-e2rhioty 41 24 . . . cord-275413-e2rhioty 42 1 One one CD cord-275413-e2rhioty 42 2 half half NN cord-275413-e2rhioty 42 3 of of IN cord-275413-e2rhioty 42 4 the the DT cord-275413-e2rhioty 42 5 inoculum inoculum NN cord-275413-e2rhioty 42 6 was be VBD cord-275413-e2rhioty 42 7 administered administer VBN cord-275413-e2rhioty 42 8 by by IN cord-275413-e2rhioty 42 9 intramuscular intramuscular JJ cord-275413-e2rhioty 42 10 injection injection NN cord-275413-e2rhioty 42 11 in in IN cord-275413-e2rhioty 42 12 the the DT cord-275413-e2rhioty 42 13 neck neck NN cord-275413-e2rhioty 42 14 . . . cord-275413-e2rhioty 43 1 The the DT cord-275413-e2rhioty 43 2 remaining remain VBG cord-275413-e2rhioty 43 3 dose dose NN cord-275413-e2rhioty 43 4 was be VBD cord-275413-e2rhioty 43 5 administered administer VBN cord-275413-e2rhioty 43 6 intranasally intranasally RB cord-275413-e2rhioty 43 7 . . . cord-275413-e2rhioty 44 1 Mock mock JJ cord-275413-e2rhioty 44 2 - - HYPH cord-275413-e2rhioty 44 3 infected infect VBN cord-275413-e2rhioty 44 4 sows sow NNS cord-275413-e2rhioty 44 5 were be VBD cord-275413-e2rhioty 44 6 challenged challenge VBN cord-275413-e2rhioty 44 7 with with IN cord-275413-e2rhioty 44 8 medium medium NN cord-275413-e2rhioty 44 9 recovered recover VBN cord-275413-e2rhioty 44 10 from from IN cord-275413-e2rhioty 44 11 MARC-145 MARC-145 NNP cord-275413-e2rhioty 44 12 cells cell NNS cord-275413-e2rhioty 44 13 . . . cord-275413-e2rhioty 45 1 Dams dam NNS cord-275413-e2rhioty 45 2 were be VBD cord-275413-e2rhioty 45 3 monitored monitor VBN cord-275413-e2rhioty 45 4 for for IN cord-275413-e2rhioty 45 5 clinical clinical JJ cord-275413-e2rhioty 45 6 signs sign NNS cord-275413-e2rhioty 45 7 and and CC cord-275413-e2rhioty 45 8 blood blood NN cord-275413-e2rhioty 45 9 collected collect VBD cord-275413-e2rhioty 45 10 weekly weekly RB cord-275413-e2rhioty 45 11 . . . cord-275413-e2rhioty 46 1 At at IN cord-275413-e2rhioty 46 2 between between IN cord-275413-e2rhioty 46 3 109 109 CD cord-275413-e2rhioty 46 4 and and CC cord-275413-e2rhioty 46 5 112 112 CD cord-275413-e2rhioty 46 6 days day NNS cord-275413-e2rhioty 46 7 of of IN cord-275413-e2rhioty 46 8 an an DT cord-275413-e2rhioty 46 9 approximate approximate JJ cord-275413-e2rhioty 46 10 114 114 CD cord-275413-e2rhioty 46 11 days day NNS cord-275413-e2rhioty 46 12 gestation gestation NN cord-275413-e2rhioty 46 13 period period NN cord-275413-e2rhioty 46 14 , , , cord-275413-e2rhioty 46 15 the the DT cord-275413-e2rhioty 46 16 dams dam NNS cord-275413-e2rhioty 46 17 were be VBD cord-275413-e2rhioty 46 18 euthanized euthanize VBN cord-275413-e2rhioty 46 19 . . . cord-275413-e2rhioty 47 1 The the DT cord-275413-e2rhioty 47 2 uterine uterine JJ cord-275413-e2rhioty 47 3 horns horn NNS cord-275413-e2rhioty 47 4 were be VBD cord-275413-e2rhioty 47 5 immediately immediately RB cord-275413-e2rhioty 47 6 removed remove VBN cord-275413-e2rhioty 47 7 and and CC cord-275413-e2rhioty 47 8 the the DT cord-275413-e2rhioty 47 9 individual individual JJ cord-275413-e2rhioty 47 10 fetuses fetus NNS cord-275413-e2rhioty 47 11 with with IN cord-275413-e2rhioty 47 12 intact intact JJ cord-275413-e2rhioty 47 13 placenta placenta NN cord-275413-e2rhioty 47 14 were be VBD cord-275413-e2rhioty 47 15 carefully carefully RB cord-275413-e2rhioty 47 16 removed remove VBN cord-275413-e2rhioty 47 17 and and CC cord-275413-e2rhioty 47 18 immediately immediately RB cord-275413-e2rhioty 47 19 necropsied necropsied JJ cord-275413-e2rhioty 47 20 . . . cord-275413-e2rhioty 48 1 A a DT cord-275413-e2rhioty 48 2 sample sample NN cord-275413-e2rhioty 48 3 of of IN cord-275413-e2rhioty 48 4 amniotic amniotic NN cord-275413-e2rhioty 48 5 fluid fluid NN cord-275413-e2rhioty 48 6 was be VBD cord-275413-e2rhioty 48 7 collected collect VBN cord-275413-e2rhioty 48 8 prior prior RB cord-275413-e2rhioty 48 9 to to IN cord-275413-e2rhioty 48 10 removal removal NN cord-275413-e2rhioty 48 11 . . . cord-275413-e2rhioty 49 1 The the DT cord-275413-e2rhioty 49 2 brachial brachial JJ cord-275413-e2rhioty 49 3 artery artery NN cord-275413-e2rhioty 49 4 of of IN cord-275413-e2rhioty 49 5 each each DT cord-275413-e2rhioty 49 6 fetus fetus NN cord-275413-e2rhioty 49 7 was be VBD cord-275413-e2rhioty 49 8 severed sever VBN cord-275413-e2rhioty 49 9 and and CC cord-275413-e2rhioty 49 10 blood blood NN cord-275413-e2rhioty 49 11 collected collect VBN cord-275413-e2rhioty 49 12 using use VBG cord-275413-e2rhioty 49 13 a a DT cord-275413-e2rhioty 49 14 disposable disposable JJ cord-275413-e2rhioty 49 15 syringe syringe NN cord-275413-e2rhioty 49 16 and and CC cord-275413-e2rhioty 49 17 serum serum NN cord-275413-e2rhioty 49 18 stored store VBN cord-275413-e2rhioty 49 19 at at IN cord-275413-e2rhioty 49 20 −80 −80 NNP cord-275413-e2rhioty 50 1 • • NNP cord-275413-e2rhioty 51 1 C. C. NNP cord-275413-e2rhioty 51 2 Maternal Maternal NNP cord-275413-e2rhioty 51 3 , , , cord-275413-e2rhioty 51 4 accessory accessory NN cord-275413-e2rhioty 51 5 and and CC cord-275413-e2rhioty 51 6 fetal fetal JJ cord-275413-e2rhioty 51 7 tissues tissue NNS cord-275413-e2rhioty 51 8 were be VBD cord-275413-e2rhioty 51 9 collected collect VBN cord-275413-e2rhioty 51 10 and and CC cord-275413-e2rhioty 51 11 stored store VBN cord-275413-e2rhioty 51 12 in in IN cord-275413-e2rhioty 51 13 formalin formalin NN cord-275413-e2rhioty 51 14 for for IN cord-275413-e2rhioty 51 15 histological histological JJ cord-275413-e2rhioty 51 16 staining staining NN cord-275413-e2rhioty 51 17 and and CC cord-275413-e2rhioty 51 18 immunohistochemistry immunohistochemistry NN cord-275413-e2rhioty 51 19 ( ( -LRB- cord-275413-e2rhioty 51 20 IHC IHC NNP cord-275413-e2rhioty 51 21 ) ) -RRB- cord-275413-e2rhioty 51 22 , , , cord-275413-e2rhioty 51 23 or or CC cord-275413-e2rhioty 51 24 storage storage NN cord-275413-e2rhioty 51 25 in in IN cord-275413-e2rhioty 51 26 RNAlater RNAlater NNP cord-275413-e2rhioty 51 27 ( ( -LRB- cord-275413-e2rhioty 51 28 Ambion Ambion NNP cord-275413-e2rhioty 51 29 ) ) -RRB- cord-275413-e2rhioty 51 30 for for IN cord-275413-e2rhioty 51 31 RT RT NNP cord-275413-e2rhioty 51 32 - - HYPH cord-275413-e2rhioty 51 33 PCR PCR NNP cord-275413-e2rhioty 51 34 of of IN cord-275413-e2rhioty 51 35 cytokine cytokine NN cord-275413-e2rhioty 51 36 mRNAs mrnas ADD cord-275413-e2rhioty 51 37 . . . cord-275413-e2rhioty 52 1 PRRSV PRRSV NNP cord-275413-e2rhioty 52 2 - - HYPH cord-275413-e2rhioty 52 3 specific specific JJ cord-275413-e2rhioty 52 4 antibody antibody NN cord-275413-e2rhioty 52 5 was be VBD cord-275413-e2rhioty 52 6 measured measure VBN cord-275413-e2rhioty 52 7 in in IN cord-275413-e2rhioty 52 8 sera sera NNP cord-275413-e2rhioty 52 9 using use VBG cord-275413-e2rhioty 52 10 the the DT cord-275413-e2rhioty 52 11 HerdCheck HerdCheck NNP cord-275413-e2rhioty 52 12 ® ® POS cord-275413-e2rhioty 52 13 PRRS PRRS NNP cord-275413-e2rhioty 52 14 ELISA ELISA NNP cord-275413-e2rhioty 52 15 ( ( -LRB- cord-275413-e2rhioty 52 16 IDEXX IDEXX NNP cord-275413-e2rhioty 52 17 ) ) -RRB- cord-275413-e2rhioty 52 18 and and CC cord-275413-e2rhioty 52 19 performed perform VBN cord-275413-e2rhioty 52 20 by by IN cord-275413-e2rhioty 52 21 personnel personnel NNS cord-275413-e2rhioty 52 22 at at IN cord-275413-e2rhioty 52 23 Kansas Kansas NNP cord-275413-e2rhioty 52 24 State State NNP cord-275413-e2rhioty 52 25 University University NNP cord-275413-e2rhioty 52 26 Veterinary Veterinary NNP cord-275413-e2rhioty 52 27 Diagnostic Diagnostic NNP cord-275413-e2rhioty 52 28 Laboratory Laboratory NNP cord-275413-e2rhioty 52 29 . . . cord-275413-e2rhioty 53 1 Serology serology NN cord-275413-e2rhioty 53 2 results result NNS cord-275413-e2rhioty 53 3 were be VBD cord-275413-e2rhioty 53 4 reported report VBN cord-275413-e2rhioty 53 5 as as IN cord-275413-e2rhioty 53 6 a a DT cord-275413-e2rhioty 53 7 sample sample NN cord-275413-e2rhioty 53 8 / / SYM cord-275413-e2rhioty 53 9 positive positive JJ cord-275413-e2rhioty 53 10 ( ( -LRB- cord-275413-e2rhioty 53 11 S s NN cord-275413-e2rhioty 53 12 / / SYM cord-275413-e2rhioty 53 13 P p NN cord-275413-e2rhioty 53 14 ) ) -RRB- cord-275413-e2rhioty 53 15 ratio ratio NN cord-275413-e2rhioty 53 16 . . . cord-275413-e2rhioty 54 1 An an DT cord-275413-e2rhioty 54 2 S S NNP cord-275413-e2rhioty 54 3 / / SYM cord-275413-e2rhioty 54 4 P p NN cord-275413-e2rhioty 54 5 ratio ratio NN cord-275413-e2rhioty 54 6 greater great JJR cord-275413-e2rhioty 54 7 than than IN cord-275413-e2rhioty 54 8 0.39 0.39 CD cord-275413-e2rhioty 54 9 was be VBD cord-275413-e2rhioty 54 10 considered consider VBN cord-275413-e2rhioty 54 11 positive positive JJ cord-275413-e2rhioty 54 12 for for IN cord-275413-e2rhioty 54 13 PRRSV PRRSV NNP cord-275413-e2rhioty 54 14 antibody antibody NN cord-275413-e2rhioty 54 15 . . . cord-275413-e2rhioty 55 1 Virus virus NN cord-275413-e2rhioty 55 2 isolation isolation NN cord-275413-e2rhioty 55 3 ( ( -LRB- cord-275413-e2rhioty 55 4 VI VI NNP cord-275413-e2rhioty 55 5 ) ) -RRB- cord-275413-e2rhioty 55 6 in in IN cord-275413-e2rhioty 55 7 serum serum NN cord-275413-e2rhioty 55 8 and and CC cord-275413-e2rhioty 55 9 tissues tissue NNS cord-275413-e2rhioty 55 10 was be VBD cord-275413-e2rhioty 55 11 performed perform VBN cord-275413-e2rhioty 55 12 as as IN cord-275413-e2rhioty 55 13 described describe VBN cord-275413-e2rhioty 55 14 in in IN cord-275413-e2rhioty 55 15 Rowland Rowland NNP cord-275413-e2rhioty 55 16 et et NNP cord-275413-e2rhioty 55 17 al al NNP cord-275413-e2rhioty 55 18 . . . cord-275413-e2rhioty 56 1 ( ( -LRB- cord-275413-e2rhioty 56 2 2003 2003 CD cord-275413-e2rhioty 56 3 ) ) -RRB- cord-275413-e2rhioty 56 4 . . . cord-275413-e2rhioty 57 1 Briefly briefly RB cord-275413-e2rhioty 57 2 , , , cord-275413-e2rhioty 57 3 serum serum NN cord-275413-e2rhioty 57 4 was be VBD cord-275413-e2rhioty 57 5 serially serially RB cord-275413-e2rhioty 57 6 diluted dilute VBN cord-275413-e2rhioty 57 7 in in IN cord-275413-e2rhioty 57 8 MEM MEM NNP cord-275413-e2rhioty 57 9 supplemented supplement VBN cord-275413-e2rhioty 57 10 with with IN cord-275413-e2rhioty 57 11 pen pen NN cord-275413-e2rhioty 57 12 / / SYM cord-275413-e2rhioty 57 13 step step NN cord-275413-e2rhioty 57 14 antibiotics antibiotic NNS cord-275413-e2rhioty 57 15 and and CC cord-275413-e2rhioty 57 16 7 7 CD cord-275413-e2rhioty 57 17 % % NN cord-275413-e2rhioty 57 18 FBS FBS NNP cord-275413-e2rhioty 57 19 and and CC cord-275413-e2rhioty 57 20 placed place VBN cord-275413-e2rhioty 57 21 on on IN cord-275413-e2rhioty 57 22 96 96 CD cord-275413-e2rhioty 57 23 well well JJ cord-275413-e2rhioty 57 24 plates plate NNS cord-275413-e2rhioty 57 25 of of IN cord-275413-e2rhioty 57 26 confluent confluent JJ cord-275413-e2rhioty 57 27 MARC-145 MARC-145 NNP cord-275413-e2rhioty 57 28 cells cell NNS cord-275413-e2rhioty 57 29 . . . cord-275413-e2rhioty 58 1 After after IN cord-275413-e2rhioty 58 2 three three CD cord-275413-e2rhioty 58 3 days day NNS cord-275413-e2rhioty 58 4 , , , cord-275413-e2rhioty 58 5 plates plate NNS cord-275413-e2rhioty 58 6 were be VBD cord-275413-e2rhioty 58 7 fixed fix VBN cord-275413-e2rhioty 58 8 in in IN cord-275413-e2rhioty 58 9 80 80 CD cord-275413-e2rhioty 58 10 % % NN cord-275413-e2rhioty 58 11 acetone acetone NN cord-275413-e2rhioty 58 12 and and CC cord-275413-e2rhioty 58 13 stained stain VBN cord-275413-e2rhioty 58 14 with with IN cord-275413-e2rhioty 58 15 FITC FITC NNP cord-275413-e2rhioty 58 16 - - HYPH cord-275413-e2rhioty 58 17 SDOW-17 SDOW-17 NNP cord-275413-e2rhioty 58 18 anti anti JJ cord-275413-e2rhioty 58 19 - - JJ cord-275413-e2rhioty 58 20 nucleocapsid nucleocapsid JJ cord-275413-e2rhioty 58 21 antibody antibody NN cord-275413-e2rhioty 58 22 , , , cord-275413-e2rhioty 58 23 diluted dilute VBN cord-275413-e2rhioty 58 24 in in IN cord-275413-e2rhioty 58 25 PBS PBS NNP cord-275413-e2rhioty 58 26 with with IN cord-275413-e2rhioty 58 27 5 5 CD cord-275413-e2rhioty 58 28 % % NN cord-275413-e2rhioty 58 29 FBS FBS NNP cord-275413-e2rhioty 58 30 ( ( -LRB- cord-275413-e2rhioty 58 31 Nelson Nelson NNP cord-275413-e2rhioty 58 32 et et NNP cord-275413-e2rhioty 58 33 al al NNP cord-275413-e2rhioty 58 34 . . NNP cord-275413-e2rhioty 58 35 , , , cord-275413-e2rhioty 58 36 1993 1993 CD cord-275413-e2rhioty 58 37 ) ) -RRB- cord-275413-e2rhioty 58 38 . . . cord-275413-e2rhioty 59 1 The the DT cord-275413-e2rhioty 59 2 results result NNS cord-275413-e2rhioty 59 3 were be VBD cord-275413-e2rhioty 59 4 reported report VBN cord-275413-e2rhioty 59 5 as as IN cord-275413-e2rhioty 59 6 the the DT cord-275413-e2rhioty 59 7 log log NN cord-275413-e2rhioty 59 8 10 10 CD cord-275413-e2rhioty 59 9 of of IN cord-275413-e2rhioty 59 10 the the DT cord-275413-e2rhioty 59 11 inverse inverse JJ cord-275413-e2rhioty 59 12 dilution dilution NN cord-275413-e2rhioty 59 13 of of IN cord-275413-e2rhioty 59 14 the the DT cord-275413-e2rhioty 59 15 last last JJ cord-275413-e2rhioty 59 16 positive positive JJ cord-275413-e2rhioty 59 17 well well RB cord-275413-e2rhioty 59 18 . . . cord-275413-e2rhioty 60 1 Virus virus NN cord-275413-e2rhioty 60 2 isolation isolation NN cord-275413-e2rhioty 60 3 from from IN cord-275413-e2rhioty 60 4 tissues tissue NNS cord-275413-e2rhioty 60 5 was be VBD cord-275413-e2rhioty 60 6 the the DT cord-275413-e2rhioty 60 7 same same JJ cord-275413-e2rhioty 60 8 except except IN cord-275413-e2rhioty 60 9 that that IN cord-275413-e2rhioty 60 10 tissues tissue NNS cord-275413-e2rhioty 60 11 were be VBD cord-275413-e2rhioty 60 12 weighed weigh VBN cord-275413-e2rhioty 60 13 and and CC cord-275413-e2rhioty 60 14 homogenized homogenize VBN cord-275413-e2rhioty 60 15 in in IN cord-275413-e2rhioty 60 16 Hanks Hanks NNP cord-275413-e2rhioty 60 17 balanced balanced JJ cord-275413-e2rhioty 60 18 salt salt NN cord-275413-e2rhioty 60 19 solution solution NN cord-275413-e2rhioty 60 20 and and CC cord-275413-e2rhioty 60 21 then then RB cord-275413-e2rhioty 60 22 centrifuged centrifuge VBN cord-275413-e2rhioty 60 23 at at IN cord-275413-e2rhioty 60 24 500 500 CD cord-275413-e2rhioty 60 25 × × NN cord-275413-e2rhioty 60 26 g g NN cord-275413-e2rhioty 60 27 for for IN cord-275413-e2rhioty 60 28 20 20 CD cord-275413-e2rhioty 60 29 min min NN cord-275413-e2rhioty 60 30 to to TO cord-275413-e2rhioty 60 31 remove remove VB cord-275413-e2rhioty 60 32 debris debris NN cord-275413-e2rhioty 60 33 . . . cord-275413-e2rhioty 61 1 The the DT cord-275413-e2rhioty 61 2 sequencing sequencing NN cord-275413-e2rhioty 61 3 of of IN cord-275413-e2rhioty 61 4 the the DT cord-275413-e2rhioty 61 5 hypervariable hypervariable JJ cord-275413-e2rhioty 61 6 region region NN cord-275413-e2rhioty 61 7 of of IN cord-275413-e2rhioty 61 8 ORF5 ORF5 NNP cord-275413-e2rhioty 61 9 is be VBZ cord-275413-e2rhioty 61 10 described describe VBN cord-275413-e2rhioty 61 11 in in IN cord-275413-e2rhioty 61 12 Rowland Rowland NNP cord-275413-e2rhioty 61 13 et et NNP cord-275413-e2rhioty 61 14 al al NNP cord-275413-e2rhioty 61 15 . . . cord-275413-e2rhioty 62 1 ( ( -LRB- cord-275413-e2rhioty 62 2 1999 1999 CD cord-275413-e2rhioty 62 3 ) ) -RRB- cord-275413-e2rhioty 62 4 . . . cord-275413-e2rhioty 63 1 Total Total NNP cord-275413-e2rhioty 63 2 RNA RNA NNP cord-275413-e2rhioty 63 3 was be VBD cord-275413-e2rhioty 63 4 prepared prepare VBN cord-275413-e2rhioty 63 5 from from IN cord-275413-e2rhioty 63 6 serum serum NN cord-275413-e2rhioty 63 7 or or CC cord-275413-e2rhioty 63 8 infected infect VBN cord-275413-e2rhioty 63 9 MARC-145 MARC-145 NNP cord-275413-e2rhioty 63 10 cells cell NNS cord-275413-e2rhioty 63 11 using use VBG cord-275413-e2rhioty 63 12 an an DT cord-275413-e2rhioty 63 13 RNeasy RNeasy NNP cord-275413-e2rhioty 63 14 kit kit NN cord-275413-e2rhioty 63 15 ( ( -LRB- cord-275413-e2rhioty 63 16 Qiagen Qiagen NNP cord-275413-e2rhioty 63 17 ) ) -RRB- cord-275413-e2rhioty 63 18 according accord VBG cord-275413-e2rhioty 63 19 manufacturer manufacturer NN cord-275413-e2rhioty 63 20 's 's POS cord-275413-e2rhioty 63 21 instructions instruction NNS cord-275413-e2rhioty 63 22 . . . cord-275413-e2rhioty 64 1 For for IN cord-275413-e2rhioty 64 2 PCR PCR NNP cord-275413-e2rhioty 64 3 , , , cord-275413-e2rhioty 64 4 cDNA cdna NN cord-275413-e2rhioty 64 5 was be VBD cord-275413-e2rhioty 64 6 prepared prepare VBN cord-275413-e2rhioty 64 7 using use VBG cord-275413-e2rhioty 64 8 MLV MLV NNP cord-275413-e2rhioty 64 9 reverse reverse NN cord-275413-e2rhioty 64 10 transcriptase transcriptase NN cord-275413-e2rhioty 64 11 ( ( -LRB- cord-275413-e2rhioty 64 12 Promega Promega NNP cord-275413-e2rhioty 64 13 ) ) -RRB- cord-275413-e2rhioty 64 14 and and CC cord-275413-e2rhioty 64 15 4MSB 4msb CD cord-275413-e2rhioty 64 16 as as IN cord-275413-e2rhioty 64 17 the the DT cord-275413-e2rhioty 64 18 primer primer NN cord-275413-e2rhioty 64 19 . . . cord-275413-e2rhioty 65 1 The the DT cord-275413-e2rhioty 65 2 sense sense NN cord-275413-e2rhioty 65 3 and and CC cord-275413-e2rhioty 65 4 antisense antisense NN cord-275413-e2rhioty 65 5 primers primer NNS cord-275413-e2rhioty 65 6 for for IN cord-275413-e2rhioty 65 7 the the DT cord-275413-e2rhioty 65 8 outer outer JJ cord-275413-e2rhioty 65 9 amplification amplification NN cord-275413-e2rhioty 65 10 were be VBD cord-275413-e2rhioty 65 11 4MSA 4msa CD cord-275413-e2rhioty 65 12 , , , cord-275413-e2rhioty 65 13 5 5 CD cord-275413-e2rhioty 65 14 -CTTCGTCCCTTCTTTTCCTCGTGG -cttcgtcccttcttttcctcgtgg SYM cord-275413-e2rhioty 65 15 , , , cord-275413-e2rhioty 65 16 and and CC cord-275413-e2rhioty 65 17 4MSB 4msb CD cord-275413-e2rhioty 65 18 , , , cord-275413-e2rhioty 65 19 5 5 CD cord-275413-e2rhioty 65 20 -CCGCTCTAGAGCCAACGATAGAGTCTGC -ccgctctagagccaacgatagagtctgc CC cord-275413-e2rhioty 65 21 , , , cord-275413-e2rhioty 65 22 respectively respectively RB cord-275413-e2rhioty 65 23 . . . cord-275413-e2rhioty 66 1 The the DT cord-275413-e2rhioty 66 2 product product NN cord-275413-e2rhioty 66 3 was be VBD cord-275413-e2rhioty 66 4 re re VBN cord-275413-e2rhioty 66 5 - - VBN cord-275413-e2rhioty 66 6 amplified amplify VBN cord-275413-e2rhioty 66 7 with with IN cord-275413-e2rhioty 66 8 a a DT cord-275413-e2rhioty 66 9 nested nested JJ cord-275413-e2rhioty 66 10 set set NN cord-275413-e2rhioty 66 11 of of IN cord-275413-e2rhioty 66 12 sense sense NN cord-275413-e2rhioty 66 13 and and CC cord-275413-e2rhioty 66 14 antisense antisense NN cord-275413-e2rhioty 66 15 primers primer NNS cord-275413-e2rhioty 66 16 , , , cord-275413-e2rhioty 66 17 04A 04a CD cord-275413-e2rhioty 66 18 , , , cord-275413-e2rhioty 66 19 5 5 CD cord-275413-e2rhioty 66 20 -ACCGTGTATGTTACCATCACAGCC -accgtgtatgttaccatcacagcc NN cord-275413-e2rhioty 66 21 and and CC cord-275413-e2rhioty 66 22 04B 04b CD cord-275413-e2rhioty 66 23 , , , cord-275413-e2rhioty 66 24 ACGGGAAAGATGACAAAACTCTCC ACGGGAAAGATGACAAAACTCTCC NNP cord-275413-e2rhioty 66 25 . . . cord-275413-e2rhioty 67 1 Thirty thirty CD cord-275413-e2rhioty 67 2 - - HYPH cord-275413-e2rhioty 67 3 two two CD cord-275413-e2rhioty 67 4 cycles cycle NNS cord-275413-e2rhioty 67 5 of of IN cord-275413-e2rhioty 67 6 amplification amplification NN cord-275413-e2rhioty 67 7 were be VBD cord-275413-e2rhioty 67 8 performed perform VBN cord-275413-e2rhioty 67 9 for for IN cord-275413-e2rhioty 67 10 each each DT cord-275413-e2rhioty 67 11 primer primer NN cord-275413-e2rhioty 67 12 pair pair NN cord-275413-e2rhioty 67 13 . . . cord-275413-e2rhioty 68 1 The the DT cord-275413-e2rhioty 68 2 conditions condition NNS cord-275413-e2rhioty 68 3 for for IN cord-275413-e2rhioty 68 4 both both DT cord-275413-e2rhioty 68 5 amplifications amplification NNS cord-275413-e2rhioty 68 6 included include VBD cord-275413-e2rhioty 68 7 a a DT cord-275413-e2rhioty 68 8 95 95 CD cord-275413-e2rhioty 68 9 • • NN cord-275413-e2rhioty 68 10 C c NN cord-275413-e2rhioty 68 11 denaturing denaturing NN cord-275413-e2rhioty 68 12 step step NN cord-275413-e2rhioty 68 13 ( ( -LRB- cord-275413-e2rhioty 68 14 25 25 CD cord-275413-e2rhioty 68 15 s s NNPS cord-275413-e2rhioty 68 16 ) ) -RRB- cord-275413-e2rhioty 68 17 , , , cord-275413-e2rhioty 68 18 a a DT cord-275413-e2rhioty 68 19 58 58 CD cord-275413-e2rhioty 68 20 • • NN cord-275413-e2rhioty 68 21 C c NN cord-275413-e2rhioty 68 22 annealing annealing NN cord-275413-e2rhioty 68 23 step step NN cord-275413-e2rhioty 68 24 ( ( -LRB- cord-275413-e2rhioty 68 25 10 10 CD cord-275413-e2rhioty 68 26 s s NNPS cord-275413-e2rhioty 68 27 ) ) -RRB- cord-275413-e2rhioty 68 28 , , , cord-275413-e2rhioty 68 29 and and CC cord-275413-e2rhioty 68 30 a a DT cord-275413-e2rhioty 68 31 74 74 CD cord-275413-e2rhioty 68 32 • • NN cord-275413-e2rhioty 68 33 C c NN cord-275413-e2rhioty 68 34 ( ( -LRB- cord-275413-e2rhioty 68 35 25 25 CD cord-275413-e2rhioty 68 36 s s NNPS cord-275413-e2rhioty 68 37 ) ) -RRB- cord-275413-e2rhioty 68 38 polymerization polymerization NN cord-275413-e2rhioty 68 39 step step NN cord-275413-e2rhioty 68 40 . . . cord-275413-e2rhioty 69 1 The the DT cord-275413-e2rhioty 69 2 final final JJ cord-275413-e2rhioty 69 3 PCR PCR NNP cord-275413-e2rhioty 69 4 product product NN cord-275413-e2rhioty 69 5 , , , cord-275413-e2rhioty 69 6 which which WDT cord-275413-e2rhioty 69 7 contained contain VBD cord-275413-e2rhioty 69 8 the the DT cord-275413-e2rhioty 69 9 last last JJ cord-275413-e2rhioty 69 10 312 312 CD cord-275413-e2rhioty 69 11 nucleotides nucleotide NNS cord-275413-e2rhioty 69 12 of of IN cord-275413-e2rhioty 69 13 ORF4 ORF4 NNP cord-275413-e2rhioty 69 14 , , , cord-275413-e2rhioty 69 15 the the DT cord-275413-e2rhioty 69 16 10 10 CD cord-275413-e2rhioty 69 17 nucleotide nucleotide JJ cord-275413-e2rhioty 69 18 untranslated untranslated JJ cord-275413-e2rhioty 69 19 region region NN cord-275413-e2rhioty 69 20 ( ( -LRB- cord-275413-e2rhioty 69 21 UTR UTR NNP cord-275413-e2rhioty 69 22 ) ) -RRB- cord-275413-e2rhioty 69 23 , , , cord-275413-e2rhioty 69 24 and and CC cord-275413-e2rhioty 69 25 the the DT cord-275413-e2rhioty 69 26 first first JJ cord-275413-e2rhioty 69 27 215 215 CD cord-275413-e2rhioty 69 28 nucleotides nucleotide NNS cord-275413-e2rhioty 69 29 of of IN cord-275413-e2rhioty 69 30 ORF5 ORF5 NNP cord-275413-e2rhioty 69 31 was be VBD cord-275413-e2rhioty 69 32 sequenced sequence VBN cord-275413-e2rhioty 69 33 directly directly RB cord-275413-e2rhioty 69 34 by by IN cord-275413-e2rhioty 69 35 automated automate VBN cord-275413-e2rhioty 69 36 DNA dna NN cord-275413-e2rhioty 69 37 sequencing sequencing NN cord-275413-e2rhioty 69 38 . . . cord-275413-e2rhioty 70 1 PCR PCR NNP cord-275413-e2rhioty 70 2 products product NNS cord-275413-e2rhioty 70 3 were be VBD cord-275413-e2rhioty 70 4 cloned clone VBN cord-275413-e2rhioty 70 5 into into IN cord-275413-e2rhioty 70 6 a a DT cord-275413-e2rhioty 70 7 pCR2.1 pCR2.1 NNP cord-275413-e2rhioty 70 8 TA TA NNP cord-275413-e2rhioty 70 9 cloning cloning NN cord-275413-e2rhioty 70 10 vector vector NN cord-275413-e2rhioty 70 11 ( ( -LRB- cord-275413-e2rhioty 70 12 Invitrogen Invitrogen NNP cord-275413-e2rhioty 70 13 ) ) -RRB- cord-275413-e2rhioty 70 14 , , , cord-275413-e2rhioty 70 15 propagated propagate VBN cord-275413-e2rhioty 70 16 in in IN cord-275413-e2rhioty 70 17 Escherichia Escherichia NNP cord-275413-e2rhioty 70 18 coli coli NNS cord-275413-e2rhioty 70 19 and and CC cord-275413-e2rhioty 70 20 individual individual JJ cord-275413-e2rhioty 70 21 plasmids plasmid NNS cord-275413-e2rhioty 70 22 sequenced sequence VBN cord-275413-e2rhioty 70 23 using use VBG cord-275413-e2rhioty 70 24 M13 M13 NNP cord-275413-e2rhioty 70 25 forward forward RB cord-275413-e2rhioty 70 26 and and CC cord-275413-e2rhioty 70 27 reverse reverse VB cord-275413-e2rhioty 70 28 primers primer NNS cord-275413-e2rhioty 70 29 . . . cord-275413-e2rhioty 71 1 Sequences sequence NNS cord-275413-e2rhioty 71 2 were be VBD cord-275413-e2rhioty 71 3 analyzed analyze VBN cord-275413-e2rhioty 71 4 using use VBG cord-275413-e2rhioty 71 5 Gene Gene NNP cord-275413-e2rhioty 71 6 Jockey Jockey NNP cord-275413-e2rhioty 71 7 II II NNP cord-275413-e2rhioty 71 8 software software NN cord-275413-e2rhioty 71 9 . . . cord-275413-e2rhioty 72 1 Tissue tissue NN cord-275413-e2rhioty 72 2 samples sample NNS cord-275413-e2rhioty 72 3 for for IN cord-275413-e2rhioty 72 4 RT RT NNP cord-275413-e2rhioty 72 5 - - HYPH cord-275413-e2rhioty 72 6 PCR PCR NNP cord-275413-e2rhioty 72 7 were be VBD cord-275413-e2rhioty 72 8 immediately immediately RB cord-275413-e2rhioty 72 9 placed place VBN cord-275413-e2rhioty 72 10 in in IN cord-275413-e2rhioty 72 11 RNA RNA NNP cord-275413-e2rhioty 72 12 - - : cord-275413-e2rhioty 72 13 Later Later NNP cord-275413-e2rhioty 72 14 ( ( -LRB- cord-275413-e2rhioty 72 15 Ambion Ambion NNP cord-275413-e2rhioty 72 16 ) ) -RRB- cord-275413-e2rhioty 72 17 and and CC cord-275413-e2rhioty 72 18 stored store VBN cord-275413-e2rhioty 72 19 at at IN cord-275413-e2rhioty 72 20 −80 −80 NNP cord-275413-e2rhioty 73 1 • • NNP cord-275413-e2rhioty 74 1 C. C. NNP cord-275413-e2rhioty 74 2 Total Total NNP cord-275413-e2rhioty 74 3 RNA RNA NNP cord-275413-e2rhioty 74 4 was be VBD cord-275413-e2rhioty 74 5 extracted extract VBN cord-275413-e2rhioty 74 6 from from IN cord-275413-e2rhioty 74 7 approximately approximately RB cord-275413-e2rhioty 74 8 50 50 CD cord-275413-e2rhioty 74 9 g g NN cord-275413-e2rhioty 74 10 of of IN cord-275413-e2rhioty 74 11 tissue tissue NN cord-275413-e2rhioty 74 12 using use VBG cord-275413-e2rhioty 74 13 RNeasy RNeasy NNP cord-275413-e2rhioty 74 14 kit kit NN cord-275413-e2rhioty 74 15 ( ( -LRB- cord-275413-e2rhioty 74 16 Qiagen Qiagen NNP cord-275413-e2rhioty 74 17 ) ) -RRB- cord-275413-e2rhioty 74 18 according accord VBG cord-275413-e2rhioty 74 19 to to IN cord-275413-e2rhioty 74 20 manufacturer manufacturer NN cord-275413-e2rhioty 74 21 's 's POS cord-275413-e2rhioty 74 22 instructions instruction NNS cord-275413-e2rhioty 74 23 . . . cord-275413-e2rhioty 75 1 The the DT cord-275413-e2rhioty 75 2 design design NN cord-275413-e2rhioty 75 3 of of IN cord-275413-e2rhioty 75 4 cytokinespecific cytokinespecific NN cord-275413-e2rhioty 75 5 primers primer NNS cord-275413-e2rhioty 75 6 and and CC cord-275413-e2rhioty 75 7 RT RT NNP cord-275413-e2rhioty 75 8 - - HYPH cord-275413-e2rhioty 75 9 PCR PCR NNP cord-275413-e2rhioty 75 10 procedures procedure NNS cord-275413-e2rhioty 75 11 were be VBD cord-275413-e2rhioty 75 12 performed perform VBN cord-275413-e2rhioty 75 13 according accord VBG cord-275413-e2rhioty 75 14 to to IN cord-275413-e2rhioty 75 15 Reddy Reddy NNP cord-275413-e2rhioty 75 16 and and CC cord-275413-e2rhioty 75 17 Wilkie Wilkie NNP cord-275413-e2rhioty 75 18 ( ( -LRB- cord-275413-e2rhioty 75 19 2000 2000 CD cord-275413-e2rhioty 75 20 ) ) -RRB- cord-275413-e2rhioty 75 21 . . . cord-275413-e2rhioty 76 1 Primer primer NN cord-275413-e2rhioty 76 2 sequences sequence NNS cord-275413-e2rhioty 76 3 are be VBP cord-275413-e2rhioty 76 4 listed list VBN cord-275413-e2rhioty 76 5 in in IN cord-275413-e2rhioty 76 6 Table Table NNP cord-275413-e2rhioty 76 7 1 1 CD cord-275413-e2rhioty 76 8 . . . cord-275413-e2rhioty 77 1 RNA RNA NNP cord-275413-e2rhioty 77 2 was be VBD cord-275413-e2rhioty 77 3 diluted dilute VBN cord-275413-e2rhioty 77 4 to to IN cord-275413-e2rhioty 77 5 a a DT cord-275413-e2rhioty 77 6 final final JJ cord-275413-e2rhioty 77 7 volume volume NN cord-275413-e2rhioty 77 8 of of IN cord-275413-e2rhioty 77 9 50 50 CD cord-275413-e2rhioty 77 10 l l NN cord-275413-e2rhioty 77 11 in in IN cord-275413-e2rhioty 77 12 nuclease nuclease DT cord-275413-e2rhioty 77 13 free free JJ cord-275413-e2rhioty 77 14 water water NN cord-275413-e2rhioty 77 15 . . . cord-275413-e2rhioty 78 1 cDNA cDNA NNP cord-275413-e2rhioty 78 2 was be VBD cord-275413-e2rhioty 78 3 prepared prepared JJ cord-275413-e2rhioty 78 4 from10 from10 NNP cord-275413-e2rhioty 78 5 l l NNP cord-275413-e2rhioty 78 6 of of IN cord-275413-e2rhioty 78 7 total total NNP cord-275413-e2rhioty 78 8 RNA RNA NNP cord-275413-e2rhioty 78 9 by by IN cord-275413-e2rhioty 78 10 reverse reverse JJ cord-275413-e2rhioty 78 11 transcription transcription NN cord-275413-e2rhioty 78 12 using use VBG cord-275413-e2rhioty 78 13 Molony Molony NNP cord-275413-e2rhioty 78 14 murine murine JJ cord-275413-e2rhioty 78 15 leukemia leukemia NN cord-275413-e2rhioty 78 16 virus virus NN cord-275413-e2rhioty 78 17 reverse reverse NN cord-275413-e2rhioty 78 18 transcriptase transcriptase NN cord-275413-e2rhioty 78 19 ( ( -LRB- cord-275413-e2rhioty 78 20 Promega Promega NNP cord-275413-e2rhioty 78 21 ) ) -RRB- cord-275413-e2rhioty 78 22 and and CC cord-275413-e2rhioty 78 23 random random JJ cord-275413-e2rhioty 78 24 hexamers hexamer NNS cord-275413-e2rhioty 78 25 as as IN cord-275413-e2rhioty 78 26 primers primer NNS cord-275413-e2rhioty 78 27 . . . cord-275413-e2rhioty 79 1 The the DT cord-275413-e2rhioty 79 2 amplification amplification NN cord-275413-e2rhioty 79 3 of of IN cord-275413-e2rhioty 79 4 ␤ ␤ CD cord-275413-e2rhioty 79 5 2 2 CD cord-275413-e2rhioty 79 6 m m NNP cord-275413-e2rhioty 79 7 mRNA mRNA NNP cord-275413-e2rhioty 79 8 was be VBD cord-275413-e2rhioty 79 9 used use VBN cord-275413-e2rhioty 79 10 as as IN cord-275413-e2rhioty 79 11 an an DT cord-275413-e2rhioty 79 12 internal internal JJ cord-275413-e2rhioty 79 13 control control NN cord-275413-e2rhioty 79 14 . . . cord-275413-e2rhioty 80 1 PCR PCR NNP cord-275413-e2rhioty 80 2 amplification amplification NN cord-275413-e2rhioty 80 3 of of IN cord-275413-e2rhioty 80 4 cytokine cytokine NN cord-275413-e2rhioty 80 5 and and CC cord-275413-e2rhioty 80 6 control control NN cord-275413-e2rhioty 80 7 cDNAs cdna NNS cord-275413-e2rhioty 80 8 consisted consist VBD cord-275413-e2rhioty 80 9 of of IN cord-275413-e2rhioty 80 10 35 35 CD cord-275413-e2rhioty 80 11 cycles cycle NNS cord-275413-e2rhioty 80 12 ( ( -LRB- cord-275413-e2rhioty 80 13 45 45 CD cord-275413-e2rhioty 80 14 s s NN cord-275413-e2rhioty 80 15 at at IN cord-275413-e2rhioty 80 16 94 94 CD cord-275413-e2rhioty 80 17 • • NN cord-275413-e2rhioty 80 18 C C NNP cord-275413-e2rhioty 80 19 , , , cord-275413-e2rhioty 80 20 45 45 CD cord-275413-e2rhioty 80 21 s s NN cord-275413-e2rhioty 80 22 at at IN cord-275413-e2rhioty 80 23 55 55 CD cord-275413-e2rhioty 80 24 • • NN cord-275413-e2rhioty 80 25 C C NNP cord-275413-e2rhioty 80 26 , , , cord-275413-e2rhioty 80 27 and and CC cord-275413-e2rhioty 80 28 45 45 CD cord-275413-e2rhioty 80 29 s s NN cord-275413-e2rhioty 80 30 at at IN cord-275413-e2rhioty 80 31 72 72 CD cord-275413-e2rhioty 81 1 • • NNP cord-275413-e2rhioty 81 2 C C NNP cord-275413-e2rhioty 81 3 ) ) -RRB- cord-275413-e2rhioty 81 4 and and CC cord-275413-e2rhioty 81 5 DNA dna NN cord-275413-e2rhioty 81 6 products product NNS cord-275413-e2rhioty 81 7 electrophoresed electrophorese VBN cord-275413-e2rhioty 81 8 on on IN cord-275413-e2rhioty 81 9 a a DT cord-275413-e2rhioty 81 10 2.0 2.0 CD cord-275413-e2rhioty 81 11 % % NN cord-275413-e2rhioty 81 12 agarose agarose NN cord-275413-e2rhioty 81 13 gel gel NN cord-275413-e2rhioty 81 14 and and CC cord-275413-e2rhioty 81 15 visualized visualize VBD cord-275413-e2rhioty 81 16 using use VBG cord-275413-e2rhioty 81 17 ethidium ethidium NN cord-275413-e2rhioty 81 18 bromide bromide NN cord-275413-e2rhioty 81 19 . . . cord-275413-e2rhioty 82 1 The the DT cord-275413-e2rhioty 82 2 identity identity NN cord-275413-e2rhioty 82 3 of of IN cord-275413-e2rhioty 82 4 the the DT cord-275413-e2rhioty 82 5 DNA dna NN cord-275413-e2rhioty 82 6 products product NNS cord-275413-e2rhioty 82 7 was be VBD cord-275413-e2rhioty 82 8 confirmed confirm VBN cord-275413-e2rhioty 82 9 by by IN cord-275413-e2rhioty 82 10 DNA dna NN cord-275413-e2rhioty 82 11 sequencing sequencing NN cord-275413-e2rhioty 82 12 . . . cord-275413-e2rhioty 83 1 Tissue tissue NN cord-275413-e2rhioty 83 2 samples sample NNS cord-275413-e2rhioty 83 3 were be VBD cord-275413-e2rhioty 83 4 collected collect VBN cord-275413-e2rhioty 83 5 and and CC cord-275413-e2rhioty 83 6 immediately immediately RB cord-275413-e2rhioty 83 7 placed place VBD cord-275413-e2rhioty 83 8 in in IN cord-275413-e2rhioty 83 9 10 10 CD cord-275413-e2rhioty 83 10 % % NN cord-275413-e2rhioty 83 11 buffered buffer VBN cord-275413-e2rhioty 83 12 formalin formalin NN cord-275413-e2rhioty 83 13 . . . cord-275413-e2rhioty 84 1 Paraffin paraffin NN cord-275413-e2rhioty 84 2 - - HYPH cord-275413-e2rhioty 84 3 embedded embed VBN cord-275413-e2rhioty 84 4 thin thin JJ cord-275413-e2rhioty 84 5 sections section NNS cord-275413-e2rhioty 84 6 were be VBD cord-275413-e2rhioty 84 7 mounted mount VBN cord-275413-e2rhioty 84 8 on on IN cord-275413-e2rhioty 84 9 slides slide NNS cord-275413-e2rhioty 84 10 , , , cord-275413-e2rhioty 84 11 deparaffinized deparaffinized JJ cord-275413-e2rhioty 84 12 and and CC cord-275413-e2rhioty 84 13 stained stain VBN cord-275413-e2rhioty 84 14 with with IN cord-275413-e2rhioty 84 15 hematoxylin hematoxylin NNP cord-275413-e2rhioty 84 16 For for IN cord-275413-e2rhioty 84 17 the the DT cord-275413-e2rhioty 84 18 detection detection NN cord-275413-e2rhioty 84 19 of of IN cord-275413-e2rhioty 84 20 PRRSV PRRSV NNP cord-275413-e2rhioty 84 21 antigen antigen NNP cord-275413-e2rhioty 84 22 , , , cord-275413-e2rhioty 84 23 slides slide NNS cord-275413-e2rhioty 84 24 were be VBD cord-275413-e2rhioty 84 25 incubated incubate VBN cord-275413-e2rhioty 84 26 for for IN cord-275413-e2rhioty 84 27 30 30 CD cord-275413-e2rhioty 84 28 min min NN cord-275413-e2rhioty 84 29 with with IN cord-275413-e2rhioty 84 30 a a DT cord-275413-e2rhioty 84 31 1:100 1:100 CD cord-275413-e2rhioty 84 32 dilution dilution NN cord-275413-e2rhioty 84 33 of of IN cord-275413-e2rhioty 84 34 mAb mAb NNP cord-275413-e2rhioty 84 35 SR-30 SR-30 NNP cord-275413-e2rhioty 84 36 anti anti JJ cord-275413-e2rhioty 84 37 - - JJ cord-275413-e2rhioty 84 38 nucleocapsid nucleocapsid JJ cord-275413-e2rhioty 84 39 antibody antibody NN cord-275413-e2rhioty 84 40 ( ( -LRB- cord-275413-e2rhioty 84 41 Rural Rural NNP cord-275413-e2rhioty 84 42 Technologies Technologies NNPS cord-275413-e2rhioty 84 43 ) ) -RRB- cord-275413-e2rhioty 84 44 . . . cord-275413-e2rhioty 85 1 Other other JJ cord-275413-e2rhioty 85 2 antibodies antibody NNS cord-275413-e2rhioty 85 3 included include VBD cord-275413-e2rhioty 85 4 a a DT cord-275413-e2rhioty 85 5 polyclonal polyclonal JJ cord-275413-e2rhioty 85 6 anti anti JJ cord-275413-e2rhioty 85 7 - - JJ cord-275413-e2rhioty 85 8 human human JJ cord-275413-e2rhioty 85 9 CD3 cd3 NN cord-275413-e2rhioty 85 10 and and CC cord-275413-e2rhioty 85 11 B B NNP cord-275413-e2rhioty 85 12 cell cell NN cord-275413-e2rhioty 85 13 antibodies antibody NNS cord-275413-e2rhioty 85 14 , , , cord-275413-e2rhioty 85 15 anti anti JJ cord-275413-e2rhioty 85 16 - - NNS cord-275413-e2rhioty 85 17 CDw75 cdw75 JJ cord-275413-e2rhioty 85 18 and and CC cord-275413-e2rhioty 85 19 anti anti JJ cord-275413-e2rhioty 85 20 - - JJ cord-275413-e2rhioty 85 21 CD79 CD79 NNP cord-275413-e2rhioty 86 1 ␣ ␣ NNP cord-275413-e2rhioty 86 2 . . . cord-275413-e2rhioty 87 1 Bound bound JJ cord-275413-e2rhioty 87 2 antibody antibody NN cord-275413-e2rhioty 87 3 was be VBD cord-275413-e2rhioty 87 4 detected detect VBN cord-275413-e2rhioty 87 5 with with IN cord-275413-e2rhioty 88 1 biotinylated biotinylated NNP cord-275413-e2rhioty 88 2 goat goat NNP cord-275413-e2rhioty 88 3 anti anti NNP cord-275413-e2rhioty 88 4 - - JJ cord-275413-e2rhioty 88 5 mouse mouse JJ cord-275413-e2rhioty 88 6 or or CC cord-275413-e2rhioty 88 7 anti anti JJ cord-275413-e2rhioty 88 8 - - JJ cord-275413-e2rhioty 88 9 rabbit rabbit JJ cord-275413-e2rhioty 88 10 Ig Ig NNPS cord-275413-e2rhioty 88 11 followed follow VBN cord-275413-e2rhioty 88 12 by by IN cord-275413-e2rhioty 88 13 avidin avidin NN cord-275413-e2rhioty 88 14 - - HYPH cord-275413-e2rhioty 88 15 HRPO HRPO NNP cord-275413-e2rhioty 88 16 and and CC cord-275413-e2rhioty 88 17 DAB DAB NNP cord-275413-e2rhioty 88 18 chromagen chromagen NNP cord-275413-e2rhioty 88 19 ( ( -LRB- cord-275413-e2rhioty 88 20 Ventana Ventana NNP cord-275413-e2rhioty 88 21 Medical Medical NNP cord-275413-e2rhioty 88 22 ) ) -RRB- cord-275413-e2rhioty 88 23 . . . cord-275413-e2rhioty 89 1 Slides slide NNS cord-275413-e2rhioty 89 2 were be VBD cord-275413-e2rhioty 89 3 counterstained counterstaine VBN cord-275413-e2rhioty 89 4 with with IN cord-275413-e2rhioty 89 5 hematoxylin hematoxylin NNP cord-275413-e2rhioty 89 6 . . . cord-275413-e2rhioty 90 1 The the DT cord-275413-e2rhioty 90 2 experiment experiment NN cord-275413-e2rhioty 90 3 incorporated incorporate VBD cord-275413-e2rhioty 90 4 four four CD cord-275413-e2rhioty 90 5 PRRSV PRRSV NNP cord-275413-e2rhioty 90 6 - - HYPH cord-275413-e2rhioty 90 7 infected infect VBN cord-275413-e2rhioty 90 8 and and CC cord-275413-e2rhioty 90 9 twomock twomock NN cord-275413-e2rhioty 90 10 - - HYPH cord-275413-e2rhioty 90 11 infected infect VBN cord-275413-e2rhioty 90 12 dams dam NNS cord-275413-e2rhioty 90 13 . . . cord-275413-e2rhioty 91 1 All all DT cord-275413-e2rhioty 91 2 maternal maternal JJ cord-275413-e2rhioty 91 3 serum serum NN cord-275413-e2rhioty 91 4 samples sample NNS cord-275413-e2rhioty 91 5 were be VBD cord-275413-e2rhioty 91 6 VI vi NN cord-275413-e2rhioty 91 7 - - HYPH cord-275413-e2rhioty 91 8 negative negative JJ cord-275413-e2rhioty 91 9 and and CC cord-275413-e2rhioty 91 10 seronegative seronegative JJ cord-275413-e2rhioty 91 11 for for IN cord-275413-e2rhioty 91 12 PRRSV PRRSV NNP cord-275413-e2rhioty 91 13 prior prior RB cord-275413-e2rhioty 91 14 to to IN cord-275413-e2rhioty 91 15 infection infection NN cord-275413-e2rhioty 91 16 . . . cord-275413-e2rhioty 92 1 Between between IN cord-275413-e2rhioty 92 2 one one CD cord-275413-e2rhioty 92 3 and and CC cord-275413-e2rhioty 92 4 two two CD cord-275413-e2rhioty 92 5 weeks week NNS cord-275413-e2rhioty 92 6 after after IN cord-275413-e2rhioty 92 7 virus virus NN cord-275413-e2rhioty 92 8 challenge challenge NN cord-275413-e2rhioty 92 9 , , , cord-275413-e2rhioty 92 10 all all DT cord-275413-e2rhioty 92 11 infected infect VBN cord-275413-e2rhioty 92 12 sows sow NNS cord-275413-e2rhioty 92 13 were be VBD cord-275413-e2rhioty 92 14 VI vi NN cord-275413-e2rhioty 92 15 - - HYPH cord-275413-e2rhioty 92 16 positive positive JJ cord-275413-e2rhioty 92 17 in in IN cord-275413-e2rhioty 92 18 serum serum NN cord-275413-e2rhioty 92 19 , , , cord-275413-e2rhioty 92 20 confirming confirm VBG cord-275413-e2rhioty 92 21 the the DT cord-275413-e2rhioty 92 22 presence presence NN cord-275413-e2rhioty 92 23 of of IN cord-275413-e2rhioty 92 24 an an DT cord-275413-e2rhioty 92 25 active active JJ cord-275413-e2rhioty 92 26 infection infection NN cord-275413-e2rhioty 92 27 . . . cord-275413-e2rhioty 93 1 By by IN cord-275413-e2rhioty 93 2 the the DT cord-275413-e2rhioty 93 3 time time NN cord-275413-e2rhioty 93 4 of of IN cord-275413-e2rhioty 93 5 necropsy necropsy JJ cord-275413-e2rhioty 93 6 , , , cord-275413-e2rhioty 93 7 the the DT cord-275413-e2rhioty 93 8 concentration concentration NN cord-275413-e2rhioty 93 9 of of IN cord-275413-e2rhioty 93 10 circulating circulate VBG cord-275413-e2rhioty 93 11 virus virus NN cord-275413-e2rhioty 93 12 in in IN cord-275413-e2rhioty 93 13 the the DT cord-275413-e2rhioty 93 14 dams dam NNS cord-275413-e2rhioty 93 15 had have VBD cord-275413-e2rhioty 93 16 dipped dip VBN cord-275413-e2rhioty 93 17 to to IN cord-275413-e2rhioty 93 18 below below IN cord-275413-e2rhioty 93 19 detectable detectable JJ cord-275413-e2rhioty 93 20 levels level NNS cord-275413-e2rhioty 93 21 by by IN cord-275413-e2rhioty 93 22 VI VI NNP cord-275413-e2rhioty 93 23 . . . cord-275413-e2rhioty 94 1 A a DT cord-275413-e2rhioty 94 2 total total NN cord-275413-e2rhioty 94 3 of of IN cord-275413-e2rhioty 94 4 44 44 CD cord-275413-e2rhioty 94 5 viable viable JJ cord-275413-e2rhioty 94 6 fetuses fetus NNS cord-275413-e2rhioty 94 7 were be VBD cord-275413-e2rhioty 94 8 recovered recover VBN cord-275413-e2rhioty 94 9 from from IN cord-275413-e2rhioty 94 10 the the DT cord-275413-e2rhioty 94 11 four four CD cord-275413-e2rhioty 94 12 infected infected JJ cord-275413-e2rhioty 94 13 dams dam NNS cord-275413-e2rhioty 94 14 ( ( -LRB- cord-275413-e2rhioty 94 15 see see VB cord-275413-e2rhioty 94 16 Table table NN cord-275413-e2rhioty 94 17 2 2 CD cord-275413-e2rhioty 94 18 for for IN cord-275413-e2rhioty 94 19 summary summary NN cord-275413-e2rhioty 94 20 ) ) -RRB- cord-275413-e2rhioty 94 21 . . . cord-275413-e2rhioty 95 1 Two two CD cord-275413-e2rhioty 95 2 fetuses fetus NNS cord-275413-e2rhioty 95 3 were be VBD cord-275413-e2rhioty 95 4 dead dead JJ cord-275413-e2rhioty 95 5 and and CC cord-275413-e2rhioty 95 6 partially partially RB cord-275413-e2rhioty 95 7 autolysed autolyse VBN cord-275413-e2rhioty 95 8 and and CC cord-275413-e2rhioty 95 9 not not RB cord-275413-e2rhioty 95 10 subjected subject VBN cord-275413-e2rhioty 95 11 to to IN cord-275413-e2rhioty 95 12 further further JJ cord-275413-e2rhioty 95 13 study study NN cord-275413-e2rhioty 95 14 . . . cord-275413-e2rhioty 96 1 10 10 CD cord-275413-e2rhioty 96 2 ( ( -LRB- cord-275413-e2rhioty 96 3 22 22 CD cord-275413-e2rhioty 96 4 % % NN cord-275413-e2rhioty 96 5 ) ) -RRB- cord-275413-e2rhioty 96 6 of of IN cord-275413-e2rhioty 96 7 the the DT cord-275413-e2rhioty 96 8 44 44 CD cord-275413-e2rhioty 96 9 viable viable JJ cord-275413-e2rhioty 96 10 fetuses fetus NNS cord-275413-e2rhioty 96 11 were be VBD cord-275413-e2rhioty 96 12 positive positive JJ cord-275413-e2rhioty 96 13 for for IN cord-275413-e2rhioty 96 14 PRRSV PRRSV NNP cord-275413-e2rhioty 96 15 by by IN cord-275413-e2rhioty 96 16 VI VI NNP cord-275413-e2rhioty 96 17 . . . cord-275413-e2rhioty 97 1 Since since IN cord-275413-e2rhioty 97 2 dams dam NNS cord-275413-e2rhioty 97 3 were be VBD cord-275413-e2rhioty 97 4 VI vi NN cord-275413-e2rhioty 97 5 - - HYPH cord-275413-e2rhioty 97 6 negative negative JJ cord-275413-e2rhioty 97 7 in in IN cord-275413-e2rhioty 97 8 blood blood NN cord-275413-e2rhioty 97 9 at at IN cord-275413-e2rhioty 97 10 the the DT cord-275413-e2rhioty 97 11 time time NN cord-275413-e2rhioty 97 12 of of IN cord-275413-e2rhioty 97 13 necropsy necropsy JJ cord-275413-e2rhioty 97 14 , , , cord-275413-e2rhioty 97 15 it -PRON- PRP cord-275413-e2rhioty 97 16 was be VBD cord-275413-e2rhioty 97 17 concluded conclude VBN cord-275413-e2rhioty 97 18 that that IN cord-275413-e2rhioty 97 19 the the DT cord-275413-e2rhioty 97 20 presence presence NN cord-275413-e2rhioty 97 21 of of IN cord-275413-e2rhioty 97 22 virus virus NN cord-275413-e2rhioty 97 23 Serology serology NN cord-275413-e2rhioty 97 24 results result NNS cord-275413-e2rhioty 97 25 , , , cord-275413-e2rhioty 97 26 shown show VBN cord-275413-e2rhioty 97 27 in in IN cord-275413-e2rhioty 97 28 parentheses parenthesis NNS cord-275413-e2rhioty 97 29 , , , cord-275413-e2rhioty 97 30 are be VBP cord-275413-e2rhioty 97 31 presented present VBN cord-275413-e2rhioty 97 32 as as IN cord-275413-e2rhioty 97 33 the the DT cord-275413-e2rhioty 97 34 S S NNP cord-275413-e2rhioty 97 35 / / SYM cord-275413-e2rhioty 97 36 P p NN cord-275413-e2rhioty 97 37 ratio ratio NN cord-275413-e2rhioty 97 38 . . . cord-275413-e2rhioty 98 1 S S NNP cord-275413-e2rhioty 98 2 / / SYM cord-275413-e2rhioty 98 3 P p NN cord-275413-e2rhioty 98 4 ratios ratio NNS cord-275413-e2rhioty 98 5 greater great JJR cord-275413-e2rhioty 98 6 than than IN cord-275413-e2rhioty 98 7 0.4 0.4 CD cord-275413-e2rhioty 98 8 were be VBD cord-275413-e2rhioty 98 9 considered consider VBN cord-275413-e2rhioty 98 10 positive positive JJ cord-275413-e2rhioty 98 11 for for IN cord-275413-e2rhioty 98 12 PRRSV PRRSV NNP cord-275413-e2rhioty 98 13 antibody antibody NN cord-275413-e2rhioty 98 14 . . . cord-275413-e2rhioty 99 1 Dead dead JJ cord-275413-e2rhioty 99 2 fetuses fetus NNS cord-275413-e2rhioty 99 3 were be VBD cord-275413-e2rhioty 99 4 partially partially RB cord-275413-e2rhioty 99 5 autolysed autolyse VBN cord-275413-e2rhioty 99 6 and and CC cord-275413-e2rhioty 99 7 not not RB cord-275413-e2rhioty 99 8 tested test VBN cord-275413-e2rhioty 99 9 . . . cord-275413-e2rhioty 100 1 Gross gross JJ cord-275413-e2rhioty 100 2 pathology pathology NN cord-275413-e2rhioty 100 3 key key NN cord-275413-e2rhioty 100 4 : : : cord-275413-e2rhioty 101 1 * * NFP cord-275413-e2rhioty 101 2 1 1 CD cord-275413-e2rhioty 101 3 , , , cord-275413-e2rhioty 101 4 partially partially RB cord-275413-e2rhioty 101 5 mummified mummify VBN cord-275413-e2rhioty 101 6 ; ; : cord-275413-e2rhioty 101 7 * * NFP cord-275413-e2rhioty 101 8 2 2 CD cord-275413-e2rhioty 101 9 , , , cord-275413-e2rhioty 101 10 non non JJ cord-275413-e2rhioty 101 11 - - JJ cord-275413-e2rhioty 101 12 viable viable JJ cord-275413-e2rhioty 101 13 fetus fetus NN cord-275413-e2rhioty 101 14 or or CC cord-275413-e2rhioty 101 15 relatively relatively RB cord-275413-e2rhioty 101 16 low low JJ cord-275413-e2rhioty 101 17 quantity quantity NN cord-275413-e2rhioty 101 18 of of IN cord-275413-e2rhioty 101 19 amniotic amniotic NN cord-275413-e2rhioty 101 20 fluid fluid NN cord-275413-e2rhioty 101 21 ; ; : cord-275413-e2rhioty 101 22 * * NFP cord-275413-e2rhioty 101 23 3 3 CD cord-275413-e2rhioty 101 24 , , , cord-275413-e2rhioty 101 25 necrotic necrotic JJ cord-275413-e2rhioty 101 26 placenta placenta NN cord-275413-e2rhioty 101 27 ; ; . cord-275413-e2rhioty 102 1 * * NFP cord-275413-e2rhioty 102 2 4 4 CD cord-275413-e2rhioty 102 3 , , , cord-275413-e2rhioty 102 4 merconium merconium NNP cord-275413-e2rhioty 102 5 - - HYPH cord-275413-e2rhioty 102 6 and and CC cord-275413-e2rhioty 102 7 / / SYM cord-275413-e2rhioty 102 8 or or CC cord-275413-e2rhioty 102 9 blood blood NN cord-275413-e2rhioty 102 10 - - HYPH cord-275413-e2rhioty 102 11 stained stain VBN cord-275413-e2rhioty 102 12 amniotic amniotic NN cord-275413-e2rhioty 102 13 fluid fluid NN cord-275413-e2rhioty 102 14 ; ; . cord-275413-e2rhioty 103 1 * * NFP cord-275413-e2rhioty 103 2 5 5 CD cord-275413-e2rhioty 103 3 , , , cord-275413-e2rhioty 103 4 small small JJ cord-275413-e2rhioty 103 5 , , , cord-275413-e2rhioty 103 6 underdeveloped underdeveloped JJ cord-275413-e2rhioty 103 7 fetus fetus NN cord-275413-e2rhioty 103 8 . . . cord-275413-e2rhioty 104 1 in in IN cord-275413-e2rhioty 104 2 the the DT cord-275413-e2rhioty 104 3 fetal fetal JJ cord-275413-e2rhioty 104 4 circulation circulation NN cord-275413-e2rhioty 104 5 was be VBD cord-275413-e2rhioty 104 6 the the DT cord-275413-e2rhioty 104 7 result result NN cord-275413-e2rhioty 104 8 of of IN cord-275413-e2rhioty 104 9 virus virus NN cord-275413-e2rhioty 104 10 replication replication NN cord-275413-e2rhioty 104 11 in in IN cord-275413-e2rhioty 104 12 the the DT cord-275413-e2rhioty 104 13 fetus fetus NN cord-275413-e2rhioty 104 14 and and CC cord-275413-e2rhioty 104 15 not not RB cord-275413-e2rhioty 104 16 from from IN cord-275413-e2rhioty 104 17 contamination contamination NN cord-275413-e2rhioty 104 18 with with IN cord-275413-e2rhioty 104 19 maternal maternal JJ cord-275413-e2rhioty 104 20 blood blood NN cord-275413-e2rhioty 104 21 . . . cord-275413-e2rhioty 105 1 ( ( -LRB- cord-275413-e2rhioty 105 2 The the DT cord-275413-e2rhioty 105 3 virus virus NN cord-275413-e2rhioty 105 4 isolation isolation NN cord-275413-e2rhioty 105 5 technique technique NN cord-275413-e2rhioty 105 6 is be VBZ cord-275413-e2rhioty 105 7 not not RB cord-275413-e2rhioty 105 8 subject subject JJ cord-275413-e2rhioty 105 9 to to IN cord-275413-e2rhioty 105 10 false false JJ cord-275413-e2rhioty 105 11 positives positive NNS cord-275413-e2rhioty 105 12 from from IN cord-275413-e2rhioty 105 13 minute minute JJ cord-275413-e2rhioty 105 14 amounts amount NNS cord-275413-e2rhioty 105 15 of of IN cord-275413-e2rhioty 105 16 cross cross NN cord-275413-e2rhioty 105 17 - - NN cord-275413-e2rhioty 105 18 contamination contamination NN cord-275413-e2rhioty 105 19 with with IN cord-275413-e2rhioty 105 20 viral viral JJ cord-275413-e2rhioty 105 21 protein protein NN cord-275413-e2rhioty 105 22 or or CC cord-275413-e2rhioty 105 23 RNA RNA NNP cord-275413-e2rhioty 105 24 . . . cord-275413-e2rhioty 105 25 ) ) -RRB- cord-275413-e2rhioty 106 1 The the DT cord-275413-e2rhioty 106 2 number number NN cord-275413-e2rhioty 106 3 of of IN cord-275413-e2rhioty 106 4 infected infected JJ cord-275413-e2rhioty 106 5 fetuses fetus NNS cord-275413-e2rhioty 106 6 in in IN cord-275413-e2rhioty 106 7 each each DT cord-275413-e2rhioty 106 8 litter litter NN cord-275413-e2rhioty 106 9 varied varied JJ cord-275413-e2rhioty 106 10 from from IN cord-275413-e2rhioty 106 11 no no DT cord-275413-e2rhioty 106 12 infected infect VBN cord-275413-e2rhioty 106 13 fetuses fetus NNS cord-275413-e2rhioty 106 14 ( ( -LRB- cord-275413-e2rhioty 106 15 dam dam VBP cord-275413-e2rhioty 106 16 no no NN cord-275413-e2rhioty 106 17 . . NN cord-275413-e2rhioty 106 18 2 2 CD cord-275413-e2rhioty 106 19 ) ) -RRB- cord-275413-e2rhioty 106 20 to to IN cord-275413-e2rhioty 106 21 five five CD cord-275413-e2rhioty 106 22 of of IN cord-275413-e2rhioty 106 23 14 14 CD cord-275413-e2rhioty 106 24 ( ( -LRB- cord-275413-e2rhioty 106 25 36 36 CD cord-275413-e2rhioty 106 26 % % NN cord-275413-e2rhioty 106 27 ) ) -RRB- cord-275413-e2rhioty 106 28 infected infect VBN cord-275413-e2rhioty 106 29 fetuses fetus NNS cord-275413-e2rhioty 106 30 for for IN cord-275413-e2rhioty 106 31 dam dam NN cord-275413-e2rhioty 106 32 no no NN cord-275413-e2rhioty 106 33 . . NN cord-275413-e2rhioty 106 34 4 4 CD cord-275413-e2rhioty 106 35 . . . cord-275413-e2rhioty 107 1 Fetuses fetus NNS cord-275413-e2rhioty 107 2 that that WDT cord-275413-e2rhioty 107 3 were be VBD cord-275413-e2rhioty 107 4 VI vi NN cord-275413-e2rhioty 107 5 - - HYPH cord-275413-e2rhioty 107 6 negative negative JJ cord-275413-e2rhioty 107 7 in in IN cord-275413-e2rhioty 107 8 serum serum NN cord-275413-e2rhioty 107 9 were be VBD cord-275413-e2rhioty 107 10 confirmed confirm VBN cord-275413-e2rhioty 107 11 as as IN cord-275413-e2rhioty 107 12 PRRSV PRRSV NNP cord-275413-e2rhioty 107 13 - - HYPH cord-275413-e2rhioty 107 14 negative negative JJ cord-275413-e2rhioty 107 15 by by IN cord-275413-e2rhioty 107 16 VI vi NN cord-275413-e2rhioty 107 17 - - HYPH cord-275413-e2rhioty 107 18 negative negative JJ cord-275413-e2rhioty 107 19 results result NNS cord-275413-e2rhioty 107 20 in in IN cord-275413-e2rhioty 107 21 placenta placenta NN cord-275413-e2rhioty 107 22 , , , cord-275413-e2rhioty 107 23 lung lung NN cord-275413-e2rhioty 107 24 , , , cord-275413-e2rhioty 107 25 lymph lymph NN cord-275413-e2rhioty 107 26 nodes node NNS cord-275413-e2rhioty 107 27 and and CC cord-275413-e2rhioty 107 28 thymus thymus NN cord-275413-e2rhioty 107 29 ( ( -LRB- cord-275413-e2rhioty 107 30 data datum NNS cord-275413-e2rhioty 107 31 not not RB cord-275413-e2rhioty 107 32 shown show VBN cord-275413-e2rhioty 107 33 ) ) -RRB- cord-275413-e2rhioty 107 34 . . . cord-275413-e2rhioty 108 1 One one CD cord-275413-e2rhioty 108 2 fetus fetus NN cord-275413-e2rhioty 108 3 , , , cord-275413-e2rhioty 108 4 1 1 CD cord-275413-e2rhioty 108 5 - - SYM cord-275413-e2rhioty 108 6 1 1 CD cord-275413-e2rhioty 108 7 , , , cord-275413-e2rhioty 108 8 was be VBD cord-275413-e2rhioty 108 9 seropositive seropositive JJ cord-275413-e2rhioty 108 10 for for IN cord-275413-e2rhioty 108 11 PRRSV PRRSV NNP cord-275413-e2rhioty 109 1 ( ( -LRB- cord-275413-e2rhioty 109 2 S S NNP cord-275413-e2rhioty 109 3 / / SYM cord-275413-e2rhioty 109 4 P p NN cord-275413-e2rhioty 109 5 ratio ratio NN cord-275413-e2rhioty 109 6 = = SYM cord-275413-e2rhioty 109 7 0.94 0.94 CD cord-275413-e2rhioty 109 8 ) ) -RRB- cord-275413-e2rhioty 109 9 . . . cord-275413-e2rhioty 110 1 Since since IN cord-275413-e2rhioty 110 2 there there EX cord-275413-e2rhioty 110 3 is be VBZ cord-275413-e2rhioty 110 4 no no DT cord-275413-e2rhioty 110 5 maternal maternal JJ cord-275413-e2rhioty 110 6 transfer transfer NN cord-275413-e2rhioty 110 7 of of IN cord-275413-e2rhioty 110 8 antibody antibody NN cord-275413-e2rhioty 110 9 from from IN cord-275413-e2rhioty 110 10 mother mother NN cord-275413-e2rhioty 110 11 to to IN cord-275413-e2rhioty 110 12 fetus fetus NN cord-275413-e2rhioty 110 13 ( ( -LRB- cord-275413-e2rhioty 110 14 Tizard Tizard NNP cord-275413-e2rhioty 110 15 , , , cord-275413-e2rhioty 110 16 1996 1996 CD cord-275413-e2rhioty 110 17 ) ) -RRB- cord-275413-e2rhioty 110 18 , , , cord-275413-e2rhioty 110 19 it -PRON- PRP cord-275413-e2rhioty 110 20 was be VBD cord-275413-e2rhioty 110 21 concluded conclude VBN cord-275413-e2rhioty 110 22 that that IN cord-275413-e2rhioty 110 23 this this DT cord-275413-e2rhioty 110 24 fetus fetus NN cord-275413-e2rhioty 110 25 generated generate VBD cord-275413-e2rhioty 110 26 an an DT cord-275413-e2rhioty 110 27 antibody antibody NN cord-275413-e2rhioty 110 28 response response NN cord-275413-e2rhioty 110 29 to to IN cord-275413-e2rhioty 110 30 PRRSV PRRSV NNP cord-275413-e2rhioty 110 31 in in IN cord-275413-e2rhioty 110 32 utero utero NNS cord-275413-e2rhioty 110 33 . . . cord-275413-e2rhioty 111 1 7 7 CD cord-275413-e2rhioty 111 2 of of IN cord-275413-e2rhioty 111 3 the the DT cord-275413-e2rhioty 111 4 10 10 CD cord-275413-e2rhioty 111 5 infected infected JJ cord-275413-e2rhioty 111 6 fetuses fetus NNS cord-275413-e2rhioty 111 7 showed show VBD cord-275413-e2rhioty 111 8 some some DT cord-275413-e2rhioty 111 9 form form NN cord-275413-e2rhioty 111 10 of of IN cord-275413-e2rhioty 111 11 gross gross JJ cord-275413-e2rhioty 111 12 pathology pathology NN cord-275413-e2rhioty 111 13 , , , cord-275413-e2rhioty 111 14 including include VBG cord-275413-e2rhioty 111 15 growth growth NN cord-275413-e2rhioty 111 16 retardation retardation NN cord-275413-e2rhioty 111 17 ( ( -LRB- cord-275413-e2rhioty 111 18 two two CD cord-275413-e2rhioty 111 19 fetuses fetus NNS cord-275413-e2rhioty 111 20 ) ) -RRB- cord-275413-e2rhioty 111 21 or or CC cord-275413-e2rhioty 111 22 reduced reduce VBN cord-275413-e2rhioty 111 23 amounts amount NNS cord-275413-e2rhioty 111 24 and/or and/or CC cord-275413-e2rhioty 111 25 merconium merconium NN cord-275413-e2rhioty 111 26 - - HYPH cord-275413-e2rhioty 111 27 stained stain VBN cord-275413-e2rhioty 111 28 amniotic amniotic NN cord-275413-e2rhioty 111 29 fluid fluid NN cord-275413-e2rhioty 111 30 ( ( -LRB- cord-275413-e2rhioty 111 31 five five CD cord-275413-e2rhioty 111 32 fetuses fetus NNS cord-275413-e2rhioty 111 33 ) ) -RRB- cord-275413-e2rhioty 111 34 . . . cord-275413-e2rhioty 112 1 Ongoing ongoing JJ cord-275413-e2rhioty 112 2 virus virus NN cord-275413-e2rhioty 112 3 replication replication NN cord-275413-e2rhioty 112 4 in in IN cord-275413-e2rhioty 112 5 the the DT cord-275413-e2rhioty 112 6 fetus fetus NN cord-275413-e2rhioty 112 7 as as IN cord-275413-e2rhioty 112 8 the the DT cord-275413-e2rhioty 112 9 source source NN cord-275413-e2rhioty 112 10 of of IN cord-275413-e2rhioty 112 11 these these DT cord-275413-e2rhioty 112 12 gross gross JJ cord-275413-e2rhioty 112 13 pathological pathological JJ cord-275413-e2rhioty 112 14 changes change NNS cord-275413-e2rhioty 112 15 is be VBZ cord-275413-e2rhioty 112 16 questionable questionable JJ cord-275413-e2rhioty 112 17 , , , cord-275413-e2rhioty 112 18 since since IN cord-275413-e2rhioty 112 19 non non JJ cord-275413-e2rhioty 112 20 - - JJ cord-275413-e2rhioty 112 21 infected infected JJ cord-275413-e2rhioty 112 22 fetuses fetus NNS cord-275413-e2rhioty 112 23 from from IN cord-275413-e2rhioty 112 24 infected infected JJ cord-275413-e2rhioty 112 25 dams dam NNS cord-275413-e2rhioty 112 26 exhibited exhibit VBD cord-275413-e2rhioty 112 27 similar similar JJ cord-275413-e2rhioty 112 28 changes change NNS cord-275413-e2rhioty 112 29 . . . cord-275413-e2rhioty 113 1 For for IN cord-275413-e2rhioty 113 2 example example NN cord-275413-e2rhioty 113 3 , , , cord-275413-e2rhioty 113 4 fetuses fetus NNS cord-275413-e2rhioty 113 5 from from IN cord-275413-e2rhioty 113 6 dam dam NN cord-275413-e2rhioty 113 7 no no NN cord-275413-e2rhioty 113 8 . . NN cord-275413-e2rhioty 113 9 2 2 LS cord-275413-e2rhioty 113 10 ( ( -LRB- cord-275413-e2rhioty 113 11 see see VB cord-275413-e2rhioty 113 12 Fig Fig NNP cord-275413-e2rhioty 113 13 . . . cord-275413-e2rhioty 114 1 1 1 LS cord-275413-e2rhioty 114 2 ) ) -RRB- cord-275413-e2rhioty 114 3 were be VBD cord-275413-e2rhioty 114 4 either either RB cord-275413-e2rhioty 114 5 merconium merconium NN cord-275413-e2rhioty 114 6 stained stain VBN cord-275413-e2rhioty 114 7 ( ( -LRB- cord-275413-e2rhioty 114 8 two two CD cord-275413-e2rhioty 114 9 fetuses fetus NNS cord-275413-e2rhioty 114 10 ) ) -RRB- cord-275413-e2rhioty 114 11 , , , cord-275413-e2rhioty 114 12 non non JJ cord-275413-e2rhioty 114 13 - - JJ cord-275413-e2rhioty 114 14 viable viable JJ cord-275413-e2rhioty 114 15 , , , cord-275413-e2rhioty 114 16 possessed possess VBD cord-275413-e2rhioty 114 17 reduced reduced JJ cord-275413-e2rhioty 114 18 amniotic amniotic NN cord-275413-e2rhioty 114 19 fluid fluid NN cord-275413-e2rhioty 114 20 levels level NNS cord-275413-e2rhioty 114 21 ( ( -LRB- cord-275413-e2rhioty 114 22 four four CD cord-275413-e2rhioty 114 23 fetuses fetus NNS cord-275413-e2rhioty 114 24 ) ) -RRB- cord-275413-e2rhioty 114 25 or or CC cord-275413-e2rhioty 114 26 small small JJ cord-275413-e2rhioty 114 27 ( ( -LRB- cord-275413-e2rhioty 114 28 one one CD cord-275413-e2rhioty 114 29 fetus fetus NN cord-275413-e2rhioty 114 30 ) ) -RRB- cord-275413-e2rhioty 114 31 . . . cord-275413-e2rhioty 115 1 Except except IN cord-275413-e2rhioty 115 2 for for IN cord-275413-e2rhioty 115 3 one one CD cord-275413-e2rhioty 115 4 autolysed autolysed JJ cord-275413-e2rhioty 115 5 fetus fetus NN cord-275413-e2rhioty 115 6 , , , cord-275413-e2rhioty 115 7 the the DT cord-275413-e2rhioty 115 8 23 23 CD cord-275413-e2rhioty 115 9 fetuses fetus NNS cord-275413-e2rhioty 115 10 from from IN cord-275413-e2rhioty 115 11 the the DT cord-275413-e2rhioty 115 12 two two CD cord-275413-e2rhioty 115 13 control control NN cord-275413-e2rhioty 115 14 dams dam NNS cord-275413-e2rhioty 115 15 showed show VBD cord-275413-e2rhioty 115 16 no no DT cord-275413-e2rhioty 115 17 evidence evidence NN cord-275413-e2rhioty 115 18 of of IN cord-275413-e2rhioty 115 19 gross gross JJ cord-275413-e2rhioty 115 20 pathology pathology NN cord-275413-e2rhioty 115 21 ( ( -LRB- cord-275413-e2rhioty 115 22 data datum NNS cord-275413-e2rhioty 115 23 not not RB cord-275413-e2rhioty 115 24 shown show VBN cord-275413-e2rhioty 115 25 ) ) -RRB- cord-275413-e2rhioty 115 26 . . . cord-275413-e2rhioty 116 1 RNA RNA NNP cord-275413-e2rhioty 116 2 viruses virus NNS cord-275413-e2rhioty 116 3 frequently frequently RB cord-275413-e2rhioty 116 4 exist exist VBP cord-275413-e2rhioty 116 5 as as IN cord-275413-e2rhioty 116 6 a a DT cord-275413-e2rhioty 116 7 heterogeneous heterogeneous JJ cord-275413-e2rhioty 116 8 population population NN cord-275413-e2rhioty 116 9 , , , cord-275413-e2rhioty 116 10 frequently frequently RB cord-275413-e2rhioty 116 11 referred refer VBD cord-275413-e2rhioty 116 12 to to IN cord-275413-e2rhioty 116 13 as as IN cord-275413-e2rhioty 116 14 a a DT cord-275413-e2rhioty 116 15 quasispecies quasispecie NNS cord-275413-e2rhioty 116 16 . . . cord-275413-e2rhioty 117 1 The the DT cord-275413-e2rhioty 117 2 appearance appearance NN cord-275413-e2rhioty 117 3 or or CC cord-275413-e2rhioty 117 4 disappearance disappearance NN cord-275413-e2rhioty 117 5 of of IN cord-275413-e2rhioty 117 6 individual individual JJ cord-275413-e2rhioty 117 7 viral viral JJ cord-275413-e2rhioty 117 8 sequences sequence NNS cord-275413-e2rhioty 117 9 within within IN cord-275413-e2rhioty 117 10 a a DT cord-275413-e2rhioty 117 11 quasispecies quasispecie NNS cord-275413-e2rhioty 117 12 population population NN cord-275413-e2rhioty 117 13 is be VBZ cord-275413-e2rhioty 117 14 often often RB cord-275413-e2rhioty 117 15 used use VBN cord-275413-e2rhioty 117 16 as as IN cord-275413-e2rhioty 117 17 evidence evidence NN cord-275413-e2rhioty 117 18 to to TO cord-275413-e2rhioty 117 19 support support VB cord-275413-e2rhioty 117 20 the the DT cord-275413-e2rhioty 117 21 existence existence NN cord-275413-e2rhioty 117 22 of of IN cord-275413-e2rhioty 117 23 positive positive JJ cord-275413-e2rhioty 117 24 or or CC cord-275413-e2rhioty 117 25 negative negative JJ cord-275413-e2rhioty 117 26 selection selection NN cord-275413-e2rhioty 117 27 during during IN cord-275413-e2rhioty 117 28 infection infection NN cord-275413-e2rhioty 117 29 ( ( -LRB- cord-275413-e2rhioty 117 30 Elena Elena NNP cord-275413-e2rhioty 117 31 et et FW cord-275413-e2rhioty 117 32 al al NNP cord-275413-e2rhioty 117 33 . . . cord-275413-e2rhioty 118 1 , , , cord-275413-e2rhioty 118 2 2000 2000 CD cord-275413-e2rhioty 118 3 ; ; : cord-275413-e2rhioty 118 4 Forns Forns NNP cord-275413-e2rhioty 118 5 et et NNP cord-275413-e2rhioty 118 6 al al NNP cord-275413-e2rhioty 118 7 . . NNP cord-275413-e2rhioty 118 8 , , , cord-275413-e2rhioty 118 9 1999 1999 CD cord-275413-e2rhioty 118 10 ; ; : cord-275413-e2rhioty 119 1 Tsibris Tsibris NNP cord-275413-e2rhioty 119 2 et et FW cord-275413-e2rhioty 119 3 al al NNP cord-275413-e2rhioty 119 4 . . NNP cord-275413-e2rhioty 119 5 , , , cord-275413-e2rhioty 119 6 2009 2009 CD cord-275413-e2rhioty 119 7 ) ) -RRB- cord-275413-e2rhioty 119 8 . . . cord-275413-e2rhioty 120 1 Mutations mutation NNS cord-275413-e2rhioty 120 2 that that WDT cord-275413-e2rhioty 120 3 appear appear VBP cord-275413-e2rhioty 120 4 in in IN cord-275413-e2rhioty 120 5 the the DT cord-275413-e2rhioty 120 6 PRRSV PRRSV NNP cord-275413-e2rhioty 120 7 genome genome NN cord-275413-e2rhioty 120 8 are be VBP cord-275413-e2rhioty 120 9 useful useful JJ cord-275413-e2rhioty 120 10 as as IN cord-275413-e2rhioty 120 11 markers marker NNS cord-275413-e2rhioty 120 12 to to TO cord-275413-e2rhioty 120 13 identify identify VB cord-275413-e2rhioty 120 14 and and CC cord-275413-e2rhioty 120 15 follow follow VB cord-275413-e2rhioty 120 16 the the DT cord-275413-e2rhioty 120 17 appearance appearance NN cord-275413-e2rhioty 120 18 and and CC cord-275413-e2rhioty 120 19 disappearance disappearance NN cord-275413-e2rhioty 120 20 of of IN cord-275413-e2rhioty 120 21 viruses virus NNS cord-275413-e2rhioty 120 22 within within IN cord-275413-e2rhioty 120 23 the the DT cord-275413-e2rhioty 120 24 population population NN cord-275413-e2rhioty 120 25 ( ( -LRB- cord-275413-e2rhioty 120 26 Allende Allende NNP cord-275413-e2rhioty 120 27 et et NNP cord-275413-e2rhioty 120 28 al al NNP cord-275413-e2rhioty 120 29 . . NNP cord-275413-e2rhioty 120 30 , , , cord-275413-e2rhioty 120 31 2000 2000 CD cord-275413-e2rhioty 120 32 ; ; : cord-275413-e2rhioty 120 33 Rowland Rowland NNP cord-275413-e2rhioty 120 34 et et NNP cord-275413-e2rhioty 120 35 al al NNP cord-275413-e2rhioty 120 36 . . NNP cord-275413-e2rhioty 120 37 , , , cord-275413-e2rhioty 120 38 1999 1999 CD cord-275413-e2rhioty 120 39 ) ) -RRB- cord-275413-e2rhioty 120 40 . . . cord-275413-e2rhioty 121 1 DNA dna NN cord-275413-e2rhioty 121 2 sequencing sequencing NN cord-275413-e2rhioty 121 3 of of IN cord-275413-e2rhioty 121 4 ORF5 ORF5 NNP cord-275413-e2rhioty 121 5 PCR PCR NNP cord-275413-e2rhioty 121 6 products product NNS cord-275413-e2rhioty 121 7 , , , cord-275413-e2rhioty 121 8 amplified amplify VBN cord-275413-e2rhioty 121 9 directly directly RB cord-275413-e2rhioty 121 10 from from IN cord-275413-e2rhioty 121 11 the the DT cord-275413-e2rhioty 121 12 sera sera NNP cord-275413-e2rhioty 121 13 of of IN cord-275413-e2rhioty 121 14 infected infected JJ cord-275413-e2rhioty 121 15 dams dam NNS cord-275413-e2rhioty 121 16 , , , cord-275413-e2rhioty 121 17 Table Table NNP cord-275413-e2rhioty 121 18 3 3 CD cord-275413-e2rhioty 121 19 Frequency Frequency NNP cord-275413-e2rhioty 121 20 of of IN cord-275413-e2rhioty 121 21 T T NNP cord-275413-e2rhioty 121 22 at at IN cord-275413-e2rhioty 121 23 nucleotide nucleotide JJ cord-275413-e2rhioty 121 24 position position NN cord-275413-e2rhioty 121 25 77 77 CD cord-275413-e2rhioty 121 26 of of IN cord-275413-e2rhioty 121 27 ORF5 ORF5 NNP cord-275413-e2rhioty 121 28 . . . cord-275413-e2rhioty 122 1 Consensus consensus NN cord-275413-e2rhioty 122 2 nucleotide nucleotide JJ cord-275413-e2rhioty 122 3 at at IN cord-275413-e2rhioty 122 4 position position NN cord-275413-e2rhioty 122 5 77 77 CD cord-275413-e2rhioty 122 6 of of IN cord-275413-e2rhioty 122 7 ORF5 ORF5 NNP cord-275413-e2rhioty 122 8 T t NN cord-275413-e2rhioty 122 9 : : : cord-275413-e2rhioty 123 1 C c NN cord-275413-e2rhioty 123 2 ratio ratio NN cord-275413-e2rhioty 123 3 in in IN cord-275413-e2rhioty 123 4 cloned clone VBN cord-275413-e2rhioty 123 5 PCR PCR NNP cord-275413-e2rhioty 123 6 products product NNS cord-275413-e2rhioty 123 7 ( ( -LRB- cord-275413-e2rhioty 123 8 percent percent NN cord-275413-e2rhioty 123 9 ) ) -RRB- cord-275413-e2rhioty 124 1 T T NNP cord-275413-e2rhioty 124 2 3/5 3/5 CD cord-275413-e2rhioty 124 3 ( ( -LRB- cord-275413-e2rhioty 124 4 60 60 CD cord-275413-e2rhioty 124 5 ) ) -RRB- cord-275413-e2rhioty 125 1 Fetus fetus NN cord-275413-e2rhioty 125 2 4 4 CD cord-275413-e2rhioty 125 3 - - SYM cord-275413-e2rhioty 125 4 09 09 CD cord-275413-e2rhioty 125 5 T T NNP cord-275413-e2rhioty 125 6 6/7 6/7 CD cord-275413-e2rhioty 125 7 ( ( -LRB- cord-275413-e2rhioty 125 8 86 86 CD cord-275413-e2rhioty 125 9 ) ) -RRB- cord-275413-e2rhioty 126 1 Fetus fetus NN cord-275413-e2rhioty 126 2 4 4 CD cord-275413-e2rhioty 126 3 - - SYM cord-275413-e2rhioty 126 4 10 10 CD cord-275413-e2rhioty 126 5 T T NNP cord-275413-e2rhioty 126 6 3/6 3/6 CD cord-275413-e2rhioty 126 7 ( ( -LRB- cord-275413-e2rhioty 126 8 50 50 CD cord-275413-e2rhioty 126 9 ) ) -RRB- cord-275413-e2rhioty 127 1 Fetus fetus NN cord-275413-e2rhioty 127 2 4 4 CD cord-275413-e2rhioty 127 3 - - SYM cord-275413-e2rhioty 127 4 11 11 CD cord-275413-e2rhioty 127 5 C c NN cord-275413-e2rhioty 127 6 1/4 1/4 CD cord-275413-e2rhioty 127 7 ( ( -LRB- cord-275413-e2rhioty 127 8 25 25 CD cord-275413-e2rhioty 127 9 ) ) -RRB- cord-275413-e2rhioty 128 1 Fetus fetus NN cord-275413-e2rhioty 128 2 4 4 CD cord-275413-e2rhioty 128 3 - - SYM cord-275413-e2rhioty 128 4 12 12 CD cord-275413-e2rhioty 128 5 T T NNP cord-275413-e2rhioty 128 6 6/6 6/6 CD cord-275413-e2rhioty 128 7 ( ( -LRB- cord-275413-e2rhioty 128 8 100 100 CD cord-275413-e2rhioty 128 9 ) ) -RRB- cord-275413-e2rhioty 129 1 Fetus fetus NN cord-275413-e2rhioty 129 2 4 4 CD cord-275413-e2rhioty 129 3 - - SYM cord-275413-e2rhioty 129 4 13 13 CD cord-275413-e2rhioty 129 5 T T NNP cord-275413-e2rhioty 129 6 5/5 5/5 CD cord-275413-e2rhioty 129 7 ( ( -LRB- cord-275413-e2rhioty 129 8 100 100 CD cord-275413-e2rhioty 129 9 ) ) -RRB- cord-275413-e2rhioty 129 10 identified identify VBD cord-275413-e2rhioty 129 11 one one CD cord-275413-e2rhioty 129 12 dam dam NN cord-275413-e2rhioty 129 13 , , , cord-275413-e2rhioty 129 14 no no NN cord-275413-e2rhioty 129 15 . . NN cord-275413-e2rhioty 129 16 4 4 CD cord-275413-e2rhioty 129 17 , , , cord-275413-e2rhioty 129 18 which which WDT cord-275413-e2rhioty 129 19 possessed possess VBD cord-275413-e2rhioty 129 20 a a DT cord-275413-e2rhioty 129 21 virus virus NN cord-275413-e2rhioty 129 22 with with IN cord-275413-e2rhioty 129 23 a a DT cord-275413-e2rhioty 129 24 mutation mutation NN cord-275413-e2rhioty 129 25 within within IN cord-275413-e2rhioty 129 26 the the DT cord-275413-e2rhioty 129 27 hypervariable hypervariable JJ cord-275413-e2rhioty 129 28 region region NN cord-275413-e2rhioty 129 29 of of IN cord-275413-e2rhioty 129 30 ORF5 ORF5 NNP cord-275413-e2rhioty 129 31 . . . cord-275413-e2rhioty 130 1 The the DT cord-275413-e2rhioty 130 2 change change NN cord-275413-e2rhioty 130 3 detected detect VBN cord-275413-e2rhioty 130 4 by by IN cord-275413-e2rhioty 130 5 sequencing sequence VBG cord-275413-e2rhioty 130 6 the the DT cord-275413-e2rhioty 130 7 PCR PCR NNP cord-275413-e2rhioty 130 8 product product NN cord-275413-e2rhioty 130 9 was be VBD cord-275413-e2rhioty 130 10 a a DT cord-275413-e2rhioty 130 11 C c NN cord-275413-e2rhioty 130 12 to to IN cord-275413-e2rhioty 130 13 T T NNP cord-275413-e2rhioty 130 14 ( ( -LRB- cord-275413-e2rhioty 130 15 U U NNP cord-275413-e2rhioty 130 16 for for IN cord-275413-e2rhioty 130 17 RNA RNA NNP cord-275413-e2rhioty 130 18 ) ) -RRB- cord-275413-e2rhioty 130 19 nucleotide nucleotide JJ cord-275413-e2rhioty 130 20 transition transition NN cord-275413-e2rhioty 130 21 at at IN cord-275413-e2rhioty 130 22 position position NN cord-275413-e2rhioty 130 23 77 77 CD cord-275413-e2rhioty 130 24 that that WDT cord-275413-e2rhioty 130 25 resulted result VBD cord-275413-e2rhioty 130 26 in in IN cord-275413-e2rhioty 130 27 a a DT cord-275413-e2rhioty 130 28 non non JJ cord-275413-e2rhioty 130 29 - - JJ cord-275413-e2rhioty 130 30 conserved conserved JJ cord-275413-e2rhioty 130 31 amino amino NN cord-275413-e2rhioty 130 32 acid acid NN cord-275413-e2rhioty 130 33 change change NN cord-275413-e2rhioty 130 34 from from IN cord-275413-e2rhioty 130 35 threonine threonine NN cord-275413-e2rhioty 130 36 to to IN cord-275413-e2rhioty 130 37 isoleucine isoleucine NNP cord-275413-e2rhioty 130 38 in in IN cord-275413-e2rhioty 130 39 GP5 GP5 NNP cord-275413-e2rhioty 130 40 . . . cord-275413-e2rhioty 131 1 The the DT cord-275413-e2rhioty 131 2 T-77 T-77 NNP cord-275413-e2rhioty 131 3 mutation mutation NN cord-275413-e2rhioty 131 4 was be VBD cord-275413-e2rhioty 131 5 not not RB cord-275413-e2rhioty 131 6 detected detect VBN cord-275413-e2rhioty 131 7 after after IN cord-275413-e2rhioty 131 8 sequencing sequence VBG cord-275413-e2rhioty 131 9 the the DT cord-275413-e2rhioty 131 10 ORF5 ORF5 NNP cord-275413-e2rhioty 131 11 PCR PCR NNP cord-275413-e2rhioty 131 12 products product NNS cord-275413-e2rhioty 131 13 from from IN cord-275413-e2rhioty 131 14 the the DT cord-275413-e2rhioty 131 15 other other JJ cord-275413-e2rhioty 131 16 dams dam NNS cord-275413-e2rhioty 131 17 ( ( -LRB- cord-275413-e2rhioty 131 18 see see VB cord-275413-e2rhioty 131 19 Table Table NNP cord-275413-e2rhioty 131 20 3 3 CD cord-275413-e2rhioty 131 21 ) ) -RRB- cord-275413-e2rhioty 131 22 . . . cord-275413-e2rhioty 132 1 PCR PCR NNP cord-275413-e2rhioty 132 2 products product NNS cord-275413-e2rhioty 132 3 were be VBD cord-275413-e2rhioty 132 4 cloned clone VBN cord-275413-e2rhioty 132 5 into into IN cord-275413-e2rhioty 132 6 a a DT cord-275413-e2rhioty 132 7 TA TA NNP cord-275413-e2rhioty 132 8 plasmid plasmid NN cord-275413-e2rhioty 132 9 and and CC cord-275413-e2rhioty 132 10 the the DT cord-275413-e2rhioty 132 11 individual individual JJ cord-275413-e2rhioty 132 12 plasmids plasmid NNS cord-275413-e2rhioty 132 13 sequenced sequence VBN cord-275413-e2rhioty 132 14 . . . cord-275413-e2rhioty 133 1 Even even RB cord-275413-e2rhioty 133 2 though though IN cord-275413-e2rhioty 133 3 T-77 T-77 NNP cord-275413-e2rhioty 133 4 was be VBD cord-275413-e2rhioty 133 5 detected detect VBN cord-275413-e2rhioty 133 6 in in IN cord-275413-e2rhioty 133 7 the the DT cord-275413-e2rhioty 133 8 PCR PCR NNP cord-275413-e2rhioty 133 9 product product NN cord-275413-e2rhioty 133 10 from from IN cord-275413-e2rhioty 133 11 dam dam NN cord-275413-e2rhioty 133 12 no no NN cord-275413-e2rhioty 133 13 . . NN cord-275413-e2rhioty 133 14 4 4 CD cord-275413-e2rhioty 133 15 , , , cord-275413-e2rhioty 133 16 the the DT cord-275413-e2rhioty 133 17 sequence sequence NN cord-275413-e2rhioty 133 18 of of IN cord-275413-e2rhioty 133 19 individual individual JJ cord-275413-e2rhioty 133 20 clones clone NNS cord-275413-e2rhioty 133 21 showed show VBD cord-275413-e2rhioty 133 22 that that IN cord-275413-e2rhioty 133 23 two two CD cord-275413-e2rhioty 133 24 of of IN cord-275413-e2rhioty 133 25 the the DT cord-275413-e2rhioty 133 26 five five CD cord-275413-e2rhioty 133 27 sequences sequence NNS cord-275413-e2rhioty 133 28 possessed possess VBD cord-275413-e2rhioty 133 29 a a DT cord-275413-e2rhioty 133 30 C c NN cord-275413-e2rhioty 133 31 at at IN cord-275413-e2rhioty 133 32 position position NN cord-275413-e2rhioty 133 33 77 77 CD cord-275413-e2rhioty 133 34 mutation mutation NN cord-275413-e2rhioty 133 35 , , , cord-275413-e2rhioty 133 36 which which WDT cord-275413-e2rhioty 133 37 indicated indicate VBD cord-275413-e2rhioty 133 38 that that IN cord-275413-e2rhioty 133 39 viruses virus NNS cord-275413-e2rhioty 133 40 with with IN cord-275413-e2rhioty 133 41 the the DT cord-275413-e2rhioty 133 42 wild wild JJ cord-275413-e2rhioty 133 43 - - HYPH cord-275413-e2rhioty 133 44 type type NN cord-275413-e2rhioty 133 45 ORF5 orf5 NN cord-275413-e2rhioty 133 46 sequence sequence NN cord-275413-e2rhioty 133 47 were be VBD cord-275413-e2rhioty 133 48 still still RB cord-275413-e2rhioty 133 49 present present JJ cord-275413-e2rhioty 133 50 in in IN cord-275413-e2rhioty 133 51 the the DT cord-275413-e2rhioty 133 52 population population NN cord-275413-e2rhioty 133 53 . . . cord-275413-e2rhioty 134 1 Whole whole JJ cord-275413-e2rhioty 134 2 PCR pcr NN cord-275413-e2rhioty 134 3 and and CC cord-275413-e2rhioty 134 4 plasmid plasmid NN cord-275413-e2rhioty 134 5 - - HYPH cord-275413-e2rhioty 134 6 cloned clone VBN cord-275413-e2rhioty 134 7 PCR PCR NNP cord-275413-e2rhioty 134 8 products product NNS cord-275413-e2rhioty 134 9 were be VBD cord-275413-e2rhioty 134 10 sequenced sequence VBN cord-275413-e2rhioty 134 11 for for IN cord-275413-e2rhioty 134 12 the the DT cord-275413-e2rhioty 134 13 five five CD cord-275413-e2rhioty 134 14 infected infected JJ cord-275413-e2rhioty 134 15 fetuses fetus NNS cord-275413-e2rhioty 134 16 from from IN cord-275413-e2rhioty 134 17 dam dam NN cord-275413-e2rhioty 134 18 no no NN cord-275413-e2rhioty 134 19 . . NN cord-275413-e2rhioty 134 20 4 4 CD cord-275413-e2rhioty 134 21 . . . cord-275413-e2rhioty 135 1 The the DT cord-275413-e2rhioty 135 2 frequency frequency NN cord-275413-e2rhioty 135 3 of of IN cord-275413-e2rhioty 135 4 the the DT cord-275413-e2rhioty 135 5 T-77 T-77 NNP cord-275413-e2rhioty 135 6 mutation mutation NN cord-275413-e2rhioty 135 7 ranged range VBD cord-275413-e2rhioty 135 8 from from IN cord-275413-e2rhioty 135 9 25 25 CD cord-275413-e2rhioty 135 10 % % NN cord-275413-e2rhioty 135 11 ( ( -LRB- cord-275413-e2rhioty 135 12 fetus fetus NN cord-275413-e2rhioty 135 13 4 4 CD cord-275413-e2rhioty 135 14 - - SYM cord-275413-e2rhioty 135 15 11 11 CD cord-275413-e2rhioty 135 16 ) ) -RRB- cord-275413-e2rhioty 135 17 to to IN cord-275413-e2rhioty 135 18 100 100 CD cord-275413-e2rhioty 135 19 % % NN cord-275413-e2rhioty 135 20 ( ( -LRB- cord-275413-e2rhioty 135 21 fetuses fetus NNS cord-275413-e2rhioty 135 22 4 4 CD cord-275413-e2rhioty 135 23 - - SYM cord-275413-e2rhioty 135 24 12 12 CD cord-275413-e2rhioty 135 25 and and CC cord-275413-e2rhioty 135 26 4 4 CD cord-275413-e2rhioty 135 27 - - SYM cord-275413-e2rhioty 135 28 13 13 CD cord-275413-e2rhioty 135 29 ) ) -RRB- cord-275413-e2rhioty 135 30 . . . cord-275413-e2rhioty 136 1 These these DT cord-275413-e2rhioty 136 2 results result NNS cord-275413-e2rhioty 136 3 indicate indicate VBP cord-275413-e2rhioty 136 4 that that IN cord-275413-e2rhioty 136 5 the the DT cord-275413-e2rhioty 136 6 fetus fetus NN cord-275413-e2rhioty 136 7 is be VBZ cord-275413-e2rhioty 136 8 capable capable JJ cord-275413-e2rhioty 136 9 of of IN cord-275413-e2rhioty 136 10 selecting select VBG cord-275413-e2rhioty 136 11 for for IN cord-275413-e2rhioty 136 12 a a DT cord-275413-e2rhioty 136 13 particular particular JJ cord-275413-e2rhioty 136 14 virus virus NN cord-275413-e2rhioty 136 15 population population NN cord-275413-e2rhioty 136 16 , , , cord-275413-e2rhioty 136 17 which which WDT cord-275413-e2rhioty 136 18 either either CC cord-275413-e2rhioty 136 19 arises arise VBZ cord-275413-e2rhioty 136 20 in in IN cord-275413-e2rhioty 136 21 the the DT cord-275413-e2rhioty 136 22 dam dam NN cord-275413-e2rhioty 136 23 or or CC cord-275413-e2rhioty 136 24 fetus fetus NN cord-275413-e2rhioty 136 25 . . . cord-275413-e2rhioty 137 1 Therefore therefore RB cord-275413-e2rhioty 137 2 , , , cord-275413-e2rhioty 137 3 fetal fetal JJ cord-275413-e2rhioty 137 4 infection infection NN cord-275413-e2rhioty 137 5 is be VBZ cord-275413-e2rhioty 137 6 a a DT cord-275413-e2rhioty 137 7 potential potential JJ cord-275413-e2rhioty 137 8 source source NN cord-275413-e2rhioty 137 9 of of IN cord-275413-e2rhioty 137 10 PRRSV PRRSV NNP cord-275413-e2rhioty 137 11 diversity diversity NN cord-275413-e2rhioty 137 12 . . . cord-275413-e2rhioty 138 1 During during IN cord-275413-e2rhioty 138 2 acute acute JJ cord-275413-e2rhioty 138 3 infection infection NN cord-275413-e2rhioty 138 4 of of IN cord-275413-e2rhioty 138 5 the the DT cord-275413-e2rhioty 138 6 postnatal postnatal JJ cord-275413-e2rhioty 138 7 pig pig NN cord-275413-e2rhioty 138 8 , , , cord-275413-e2rhioty 138 9 the the DT cord-275413-e2rhioty 138 10 largest large JJS cord-275413-e2rhioty 138 11 quantity quantity NN cord-275413-e2rhioty 138 12 of of IN cord-275413-e2rhioty 138 13 virus virus NN cord-275413-e2rhioty 138 14 and and CC cord-275413-e2rhioty 138 15 greatest great JJS cord-275413-e2rhioty 138 16 number number NN cord-275413-e2rhioty 138 17 of of IN cord-275413-e2rhioty 138 18 cells cell NNS cord-275413-e2rhioty 138 19 supporting support VBG cord-275413-e2rhioty 138 20 virus virus NN cord-275413-e2rhioty 138 21 replication replication NN cord-275413-e2rhioty 138 22 are be VBP cord-275413-e2rhioty 138 23 found find VBN cord-275413-e2rhioty 138 24 in in IN cord-275413-e2rhioty 138 25 the the DT cord-275413-e2rhioty 138 26 lung lung NN cord-275413-e2rhioty 138 27 , , , cord-275413-e2rhioty 138 28 a a DT cord-275413-e2rhioty 138 29 consequence consequence NN cord-275413-e2rhioty 138 30 of of IN cord-275413-e2rhioty 138 31 targeting target VBG cord-275413-e2rhioty 138 32 alveolar alveolar NN cord-275413-e2rhioty 138 33 macrophages macrophage NNS cord-275413-e2rhioty 138 34 . . . cord-275413-e2rhioty 139 1 During during IN cord-275413-e2rhioty 139 2 the the DT cord-275413-e2rhioty 139 3 later later JJ cord-275413-e2rhioty 139 4 stages stage NNS cord-275413-e2rhioty 139 5 of of IN cord-275413-e2rhioty 139 6 PRRSV PRRSV NNP cord-275413-e2rhioty 139 7 infection infection NN cord-275413-e2rhioty 139 8 , , , cord-275413-e2rhioty 139 9 secondary secondary JJ cord-275413-e2rhioty 139 10 lymphoid lymphoid JJ cord-275413-e2rhioty 139 11 organs organ NNS cord-275413-e2rhioty 139 12 , , , cord-275413-e2rhioty 139 13 including include VBG cord-275413-e2rhioty 139 14 tonsil tonsil NN cord-275413-e2rhioty 139 15 and and CC cord-275413-e2rhioty 139 16 lymph lymph NN cord-275413-e2rhioty 139 17 nodes node NNS cord-275413-e2rhioty 139 18 , , , cord-275413-e2rhioty 139 19 become become VB cord-275413-e2rhioty 139 20 sources source NNS cord-275413-e2rhioty 139 21 of of IN cord-275413-e2rhioty 139 22 virus virus NN cord-275413-e2rhioty 139 23 replication replication NN cord-275413-e2rhioty 139 24 ( ( -LRB- cord-275413-e2rhioty 139 25 Allende Allende NNP cord-275413-e2rhioty 139 26 et et NNP cord-275413-e2rhioty 139 27 al al NNP cord-275413-e2rhioty 139 28 . . NNP cord-275413-e2rhioty 139 29 , , , cord-275413-e2rhioty 139 30 2000 2000 CD cord-275413-e2rhioty 139 31 ; ; : cord-275413-e2rhioty 139 32 Rossow Rossow NNP cord-275413-e2rhioty 139 33 , , , cord-275413-e2rhioty 139 34 1998 1998 CD cord-275413-e2rhioty 139 35 ; ; : cord-275413-e2rhioty 139 36 Rowland Rowland NNP cord-275413-e2rhioty 139 37 et et NNP cord-275413-e2rhioty 139 38 al al NNP cord-275413-e2rhioty 139 39 . . NNP cord-275413-e2rhioty 139 40 , , , cord-275413-e2rhioty 139 41 1999 1999 CD cord-275413-e2rhioty 139 42 ; ; : cord-275413-e2rhioty 139 43 . . . cord-275413-e2rhioty 140 1 Virus virus NN cord-275413-e2rhioty 140 2 replication replication NN cord-275413-e2rhioty 140 3 in in IN cord-275413-e2rhioty 140 4 fetal fetal JJ cord-275413-e2rhioty 140 5 tissues tissue NNS cord-275413-e2rhioty 140 6 was be VBD cord-275413-e2rhioty 140 7 assessed assess VBN cord-275413-e2rhioty 140 8 using use VBG cord-275413-e2rhioty 140 9 a a DT cord-275413-e2rhioty 140 10 combination combination NN cord-275413-e2rhioty 140 11 of of IN cord-275413-e2rhioty 140 12 virus virus NN cord-275413-e2rhioty 140 13 isolation isolation NN cord-275413-e2rhioty 140 14 and and CC cord-275413-e2rhioty 140 15 IHC IHC NNP cord-275413-e2rhioty 140 16 detection detection NN cord-275413-e2rhioty 140 17 of of IN cord-275413-e2rhioty 140 18 nucleocapsid nucleocapsid NN cord-275413-e2rhioty 140 19 antigen antigen NN cord-275413-e2rhioty 140 20 in in IN cord-275413-e2rhioty 140 21 formalin formalin NN cord-275413-e2rhioty 140 22 - - HYPH cord-275413-e2rhioty 140 23 fixed fix VBN cord-275413-e2rhioty 140 24 tissues tissue NNS cord-275413-e2rhioty 140 25 . . . cord-275413-e2rhioty 141 1 As as IN cord-275413-e2rhioty 141 2 summarized summarize VBN cord-275413-e2rhioty 141 3 in in IN cord-275413-e2rhioty 141 4 Table Table NNP cord-275413-e2rhioty 141 5 4 4 CD cord-275413-e2rhioty 141 6 , , , cord-275413-e2rhioty 141 7 virus virus NN cord-275413-e2rhioty 141 8 was be VBD cord-275413-e2rhioty 141 9 isolated isolate VBN cord-275413-e2rhioty 141 10 from from IN cord-275413-e2rhioty 141 11 all all DT cord-275413-e2rhioty 141 12 tissues tissue NNS cord-275413-e2rhioty 141 13 from from IN cord-275413-e2rhioty 141 14 infected infected JJ cord-275413-e2rhioty 141 15 fetuses fetus NNS cord-275413-e2rhioty 141 16 , , , cord-275413-e2rhioty 141 17 including include VBG cord-275413-e2rhioty 141 18 placenta placenta NN cord-275413-e2rhioty 141 19 , , , cord-275413-e2rhioty 141 20 umbilical umbilical JJ cord-275413-e2rhioty 141 21 cord cord NN cord-275413-e2rhioty 141 22 , , , cord-275413-e2rhioty 141 23 heart heart NN cord-275413-e2rhioty 141 24 , , , cord-275413-e2rhioty 141 25 lung lung NN cord-275413-e2rhioty 141 26 , , , cord-275413-e2rhioty 141 27 spleen spleen NNS cord-275413-e2rhioty 141 28 , , , cord-275413-e2rhioty 141 29 lymph lymph NN cord-275413-e2rhioty 141 30 nodes node NNS cord-275413-e2rhioty 141 31 and and CC cord-275413-e2rhioty 141 32 thymus thymus NN cord-275413-e2rhioty 141 33 . . . cord-275413-e2rhioty 142 1 Overall overall RB cord-275413-e2rhioty 142 2 , , , cord-275413-e2rhioty 142 3 the the DT cord-275413-e2rhioty 142 4 thymus thymus NN cord-275413-e2rhioty 142 5 contained contain VBD cord-275413-e2rhioty 142 6 the the DT cord-275413-e2rhioty 142 7 largest large JJS cord-275413-e2rhioty 142 8 quantity quantity NN cord-275413-e2rhioty 142 9 of of IN cord-275413-e2rhioty 142 10 virus virus NN cord-275413-e2rhioty 142 11 . . . cord-275413-e2rhioty 143 1 Nine nine CD cord-275413-e2rhioty 143 2 of of IN cord-275413-e2rhioty 143 3 ten ten CD cord-275413-e2rhioty 143 4 fetuses fetus NNS cord-275413-e2rhioty 143 5 yielded yield VBD cord-275413-e2rhioty 143 6 measurable measurable JJ cord-275413-e2rhioty 143 7 amounts amount NNS cord-275413-e2rhioty 143 8 of of IN cord-275413-e2rhioty 143 9 virus virus NN cord-275413-e2rhioty 143 10 with with IN cord-275413-e2rhioty 143 11 five five CD cord-275413-e2rhioty 143 12 of of IN cord-275413-e2rhioty 143 13 the the DT cord-275413-e2rhioty 143 14 ten ten CD cord-275413-e2rhioty 143 15 fetal fetal JJ cord-275413-e2rhioty 143 16 thymuses thymus NNS cord-275413-e2rhioty 143 17 producing produce VBG cord-275413-e2rhioty 143 18 titers titer NNS cord-275413-e2rhioty 143 19 greater great JJR cord-275413-e2rhioty 143 20 than than IN cord-275413-e2rhioty 143 21 3.0 3.0 CD cord-275413-e2rhioty 143 22 . . . cord-275413-e2rhioty 144 1 For for IN cord-275413-e2rhioty 144 2 lung lung NN cord-275413-e2rhioty 144 3 , , , cord-275413-e2rhioty 144 4 6 6 CD cord-275413-e2rhioty 144 5 of of IN cord-275413-e2rhioty 144 6 10 10 CD cord-275413-e2rhioty 144 7 fetuses fetus NNS cord-275413-e2rhioty 144 8 were be VBD cord-275413-e2rhioty 144 9 VI vi NN cord-275413-e2rhioty 144 10 - - HYPH cord-275413-e2rhioty 144 11 positive positive JJ cord-275413-e2rhioty 144 12 and and CC cord-275413-e2rhioty 144 13 only only RB cord-275413-e2rhioty 144 14 one one CD cord-275413-e2rhioty 144 15 fetal fetal JJ cord-275413-e2rhioty 144 16 lung lung NN cord-275413-e2rhioty 144 17 yielded yield VBD cord-275413-e2rhioty 144 18 a a DT cord-275413-e2rhioty 144 19 virus virus NN cord-275413-e2rhioty 144 20 titer titer NN cord-275413-e2rhioty 144 21 greater great JJR cord-275413-e2rhioty 144 22 than than IN cord-275413-e2rhioty 144 23 2.0 2.0 CD cord-275413-e2rhioty 144 24 . . . cord-275413-e2rhioty 145 1 The the DT cord-275413-e2rhioty 145 2 recovery recovery NN cord-275413-e2rhioty 145 3 of of IN cord-275413-e2rhioty 145 4 virus virus NN cord-275413-e2rhioty 145 5 from from IN cord-275413-e2rhioty 145 6 a a DT cord-275413-e2rhioty 145 7 tissue tissue NN cord-275413-e2rhioty 145 8 can can MD cord-275413-e2rhioty 145 9 represent represent VB cord-275413-e2rhioty 145 10 virus virus NN cord-275413-e2rhioty 145 11 in in IN cord-275413-e2rhioty 145 12 circulating circulate VBG cord-275413-e2rhioty 145 13 blood blood NN cord-275413-e2rhioty 145 14 . . . cord-275413-e2rhioty 146 1 Therefore therefore RB cord-275413-e2rhioty 146 2 , , , cord-275413-e2rhioty 146 3 to to TO cord-275413-e2rhioty 146 4 determine determine VB cord-275413-e2rhioty 146 5 if if IN cord-275413-e2rhioty 146 6 cells cell NNS cord-275413-e2rhioty 146 7 in in IN cord-275413-e2rhioty 146 8 the the DT cord-275413-e2rhioty 146 9 thymus thymus NN cord-275413-e2rhioty 146 10 and and CC cord-275413-e2rhioty 146 11 other other JJ cord-275413-e2rhioty 146 12 tissues tissue NNS cord-275413-e2rhioty 146 13 were be VBD cord-275413-e2rhioty 146 14 a a DT cord-275413-e2rhioty 146 15 source source NN cord-275413-e2rhioty 146 16 of of IN cord-275413-e2rhioty 146 17 PRRSV PRRSV NNP cord-275413-e2rhioty 146 18 , , , cord-275413-e2rhioty 146 19 tissue tissue NN cord-275413-e2rhioty 146 20 thin thin JJ cord-275413-e2rhioty 146 21 sections section NNS cord-275413-e2rhioty 146 22 were be VBD cord-275413-e2rhioty 146 23 stained stain VBN cord-275413-e2rhioty 146 24 with with IN cord-275413-e2rhioty 146 25 PRRSV PRRSV NNP cord-275413-e2rhioty 146 26 anti anti NNP cord-275413-e2rhioty 146 27 - - JJ cord-275413-e2rhioty 146 28 nucleocapsid nucleocapsid JJ cord-275413-e2rhioty 146 29 antibody antibody NN cord-275413-e2rhioty 146 30 . . . cord-275413-e2rhioty 147 1 The the DT cord-275413-e2rhioty 147 2 IHC IHC NNP cord-275413-e2rhioty 147 3 staining staining NN cord-275413-e2rhioty 147 4 procedure procedure NN cord-275413-e2rhioty 147 5 included include VBD cord-275413-e2rhioty 147 6 two two CD cord-275413-e2rhioty 147 7 sets set NNS cord-275413-e2rhioty 147 8 of of IN cord-275413-e2rhioty 147 9 negative negative JJ cord-275413-e2rhioty 147 10 controls control NNS cord-275413-e2rhioty 147 11 : : : cord-275413-e2rhioty 147 12 tissue tissue NN cord-275413-e2rhioty 147 13 thin thin JJ cord-275413-e2rhioty 147 14 sections section NNS cord-275413-e2rhioty 147 15 from from IN cord-275413-e2rhioty 147 16 non non JJ cord-275413-e2rhioty 147 17 - - JJ cord-275413-e2rhioty 147 18 infected infected JJ cord-275413-e2rhioty 147 19 fetuses fetus NNS cord-275413-e2rhioty 147 20 and and CC cord-275413-e2rhioty 147 21 from from IN cord-275413-e2rhioty 147 22 infected infected JJ cord-275413-e2rhioty 147 23 fetuses fetus NNS cord-275413-e2rhioty 147 24 stained stain VBN cord-275413-e2rhioty 147 25 with with IN cord-275413-e2rhioty 147 26 only only JJ cord-275413-e2rhioty 147 27 secondary secondary JJ cord-275413-e2rhioty 147 28 antibody antibody NN cord-275413-e2rhioty 147 29 . . . cord-275413-e2rhioty 148 1 Both both DT cord-275413-e2rhioty 148 2 controls control NNS cord-275413-e2rhioty 148 3 were be VBD cord-275413-e2rhioty 148 4 negative negative JJ cord-275413-e2rhioty 148 5 for for IN cord-275413-e2rhioty 148 6 staining stain VBG cord-275413-e2rhioty 148 7 ( ( -LRB- cord-275413-e2rhioty 148 8 data datum NNS cord-275413-e2rhioty 148 9 not not RB cord-275413-e2rhioty 148 10 shown show VBN cord-275413-e2rhioty 148 11 ) ) -RRB- cord-275413-e2rhioty 148 12 . . . cord-275413-e2rhioty 149 1 The the DT cord-275413-e2rhioty 149 2 results result NNS cord-275413-e2rhioty 149 3 in in IN cord-275413-e2rhioty 149 4 Table Table NNP cord-275413-e2rhioty 149 5 4 4 CD cord-275413-e2rhioty 149 6 showed show VBD cord-275413-e2rhioty 149 7 the the DT cord-275413-e2rhioty 149 8 largest large JJS cord-275413-e2rhioty 149 9 number number NN cord-275413-e2rhioty 149 10 of of IN cord-275413-e2rhioty 149 11 positive positive JJ cord-275413-e2rhioty 149 12 tissues tissue NNS cord-275413-e2rhioty 149 13 for for IN cord-275413-e2rhioty 149 14 the the DT cord-275413-e2rhioty 149 15 thymus thymus NN cord-275413-e2rhioty 149 16 ( ( -LRB- cord-275413-e2rhioty 149 17 8 8 CD cord-275413-e2rhioty 149 18 of of IN cord-275413-e2rhioty 149 19 10 10 CD cord-275413-e2rhioty 149 20 positive positive JJ cord-275413-e2rhioty 149 21 ) ) -RRB- cord-275413-e2rhioty 149 22 , , , cord-275413-e2rhioty 149 23 followed follow VBN cord-275413-e2rhioty 149 24 by by IN cord-275413-e2rhioty 149 25 spleen spleen NNS cord-275413-e2rhioty 149 26 ( ( -LRB- cord-275413-e2rhioty 149 27 2 2 CD cord-275413-e2rhioty 149 28 of of IN cord-275413-e2rhioty 149 29 8 8 CD cord-275413-e2rhioty 149 30 positive positive JJ cord-275413-e2rhioty 149 31 ) ) -RRB- cord-275413-e2rhioty 149 32 and and CC cord-275413-e2rhioty 149 33 lymph lymph NN cord-275413-e2rhioty 149 34 node node NN cord-275413-e2rhioty 149 35 ( ( -LRB- cord-275413-e2rhioty 149 36 1 1 CD cord-275413-e2rhioty 149 37 of of IN cord-275413-e2rhioty 149 38 9 9 CD cord-275413-e2rhioty 149 39 positive positive JJ cord-275413-e2rhioty 149 40 ) ) -RRB- cord-275413-e2rhioty 149 41 . . . cord-275413-e2rhioty 150 1 Within within IN cord-275413-e2rhioty 150 2 the the DT cord-275413-e2rhioty 150 3 thymus thymus NN cord-275413-e2rhioty 150 4 , , , cord-275413-e2rhioty 150 5 antigen antigen NN cord-275413-e2rhioty 150 6 - - HYPH cord-275413-e2rhioty 150 7 positive positive JJ cord-275413-e2rhioty 150 8 cells cell NNS cord-275413-e2rhioty 150 9 were be VBD cord-275413-e2rhioty 150 10 located locate VBN cord-275413-e2rhioty 150 11 in in IN cord-275413-e2rhioty 150 12 both both DT cord-275413-e2rhioty 150 13 medullar medullar JJ cord-275413-e2rhioty 150 14 and and CC cord-275413-e2rhioty 150 15 cortical cortical JJ cord-275413-e2rhioty 150 16 regions region NNS cord-275413-e2rhioty 150 17 ( ( -LRB- cord-275413-e2rhioty 150 18 data datum NNS cord-275413-e2rhioty 150 19 not not RB cord-275413-e2rhioty 150 20 shown show VBN cord-275413-e2rhioty 150 21 ) ) -RRB- cord-275413-e2rhioty 150 22 . . . cord-275413-e2rhioty 151 1 PRRSV PRRSV NNP cord-275413-e2rhioty 151 2 antigen antigen NN cord-275413-e2rhioty 151 3 - - HYPH cord-275413-e2rhioty 151 4 positive positive JJ cord-275413-e2rhioty 151 5 cells cell NNS cord-275413-e2rhioty 151 6 were be VBD cord-275413-e2rhioty 151 7 not not RB cord-275413-e2rhioty 151 8 detected detect VBN cord-275413-e2rhioty 151 9 in in IN cord-275413-e2rhioty 151 10 lung lung NN cord-275413-e2rhioty 151 11 or or CC cord-275413-e2rhioty 151 12 tonsil tonsil NN cord-275413-e2rhioty 151 13 . . . cord-275413-e2rhioty 152 1 Taken take VBN cord-275413-e2rhioty 152 2 together together RB cord-275413-e2rhioty 152 3 , , , cord-275413-e2rhioty 152 4 the the DT cord-275413-e2rhioty 152 5 virus virus NN cord-275413-e2rhioty 152 6 titration titration NN cord-275413-e2rhioty 152 7 and and CC cord-275413-e2rhioty 152 8 IHC IHC NNP cord-275413-e2rhioty 152 9 results result NNS cord-275413-e2rhioty 152 10 identify identify VBP cord-275413-e2rhioty 152 11 the the DT cord-275413-e2rhioty 152 12 thymus thymus NN cord-275413-e2rhioty 152 13 as as IN cord-275413-e2rhioty 152 14 a a DT cord-275413-e2rhioty 152 15 principal principal JJ cord-275413-e2rhioty 152 16 source source NN cord-275413-e2rhioty 152 17 of of IN cord-275413-e2rhioty 152 18 virus virus NN cord-275413-e2rhioty 152 19 replication replication NN cord-275413-e2rhioty 152 20 in in IN cord-275413-e2rhioty 152 21 the the DT cord-275413-e2rhioty 152 22 PRRSVinfected prrsvinfected JJ cord-275413-e2rhioty 152 23 fetus fetus NN cord-275413-e2rhioty 152 24 . . . cord-275413-e2rhioty 153 1 In in IN cord-275413-e2rhioty 153 2 the the DT cord-275413-e2rhioty 153 3 post post JJ cord-275413-e2rhioty 153 4 - - JJ cord-275413-e2rhioty 153 5 natal natal JJ cord-275413-e2rhioty 153 6 pig pig NN cord-275413-e2rhioty 153 7 , , , cord-275413-e2rhioty 153 8 PRRSV PRRSV NNP cord-275413-e2rhioty 153 9 infection infection NN cord-275413-e2rhioty 153 10 results result VBZ cord-275413-e2rhioty 153 11 in in IN cord-275413-e2rhioty 153 12 distinct distinct JJ cord-275413-e2rhioty 153 13 pathology pathology NN cord-275413-e2rhioty 153 14 in in IN cord-275413-e2rhioty 153 15 the the DT cord-275413-e2rhioty 153 16 lung lung NN cord-275413-e2rhioty 153 17 , , , cord-275413-e2rhioty 153 18 including include VBG cord-275413-e2rhioty 153 19 the the DT cord-275413-e2rhioty 153 20 appearance appearance NN cord-275413-e2rhioty 153 21 of of IN cord-275413-e2rhioty 153 22 interstitial interstitial JJ cord-275413-e2rhioty 153 23 pneumonia pneumonia NN cord-275413-e2rhioty 153 24 . . . cord-275413-e2rhioty 154 1 Representative representative JJ cord-275413-e2rhioty 154 2 lymph lymph NN cord-275413-e2rhioty 154 3 node node NN cord-275413-e2rhioty 154 4 and and CC cord-275413-e2rhioty 154 5 lung lung NN cord-275413-e2rhioty 154 6 tissues tissue NNS cord-275413-e2rhioty 154 7 from from IN cord-275413-e2rhioty 154 8 non non JJ cord-275413-e2rhioty 154 9 - - JJ cord-275413-e2rhioty 154 10 infected infected JJ cord-275413-e2rhioty 154 11 and and CC cord-275413-e2rhioty 154 12 infected infected JJ cord-275413-e2rhioty 154 13 fetuses fetus NNS cord-275413-e2rhioty 154 14 are be VBP cord-275413-e2rhioty 154 15 shown show VBN cord-275413-e2rhioty 154 16 in in IN cord-275413-e2rhioty 154 17 Fig Fig NNP cord-275413-e2rhioty 154 18 . . . cord-275413-e2rhioty 155 1 2 2 LS cord-275413-e2rhioty 155 2 . . . cord-275413-e2rhioty 156 1 There there EX cord-275413-e2rhioty 156 2 was be VBD cord-275413-e2rhioty 156 3 no no DT cord-275413-e2rhioty 156 4 discernable discernable JJ cord-275413-e2rhioty 156 5 difference difference NN cord-275413-e2rhioty 156 6 between between IN cord-275413-e2rhioty 156 7 lungs lung NNS cord-275413-e2rhioty 156 8 from from IN cord-275413-e2rhioty 156 9 infected infected JJ cord-275413-e2rhioty 156 10 and and CC cord-275413-e2rhioty 156 11 non non JJ cord-275413-e2rhioty 156 12 - - JJ cord-275413-e2rhioty 156 13 infected infected JJ cord-275413-e2rhioty 156 14 fetuses fetus NNS cord-275413-e2rhioty 156 15 ( ( -LRB- cord-275413-e2rhioty 156 16 compare compare VB cord-275413-e2rhioty 156 17 Fig Fig NNP cord-275413-e2rhioty 156 18 . . . cord-275413-e2rhioty 157 1 2 2 CD cord-275413-e2rhioty 157 2 panels panel NNS cord-275413-e2rhioty 157 3 C c NN cord-275413-e2rhioty 157 4 and and CC cord-275413-e2rhioty 157 5 D D NNP cord-275413-e2rhioty 157 6 ) ) -RRB- cord-275413-e2rhioty 157 7 . . . cord-275413-e2rhioty 158 1 Lymph lymph NN cord-275413-e2rhioty 158 2 nodes node NNS cord-275413-e2rhioty 158 3 from from IN cord-275413-e2rhioty 158 4 PRRSV prrsv NN cord-275413-e2rhioty 158 5 - - HYPH cord-275413-e2rhioty 158 6 negative negative JJ cord-275413-e2rhioty 158 7 fetuses fetus NNS cord-275413-e2rhioty 158 8 appeared appear VBD cord-275413-e2rhioty 158 9 largely largely RB cord-275413-e2rhioty 158 10 undeveloped undeveloped JJ cord-275413-e2rhioty 158 11 and and CC cord-275413-e2rhioty 158 12 devoid devoid JJ cord-275413-e2rhioty 158 13 of of IN cord-275413-e2rhioty 158 14 well well RB cord-275413-e2rhioty 158 15 - - HYPH cord-275413-e2rhioty 158 16 defined define VBN cord-275413-e2rhioty 158 17 germinal germinal JJ cord-275413-e2rhioty 158 18 centers center NNS cord-275413-e2rhioty 158 19 . . . cord-275413-e2rhioty 159 1 In in IN cord-275413-e2rhioty 159 2 contrast contrast NN cord-275413-e2rhioty 159 3 , , , cord-275413-e2rhioty 159 4 the the DT cord-275413-e2rhioty 159 5 lymph lymph NN cord-275413-e2rhioty 159 6 nodes node NNS cord-275413-e2rhioty 159 7 from from IN cord-275413-e2rhioty 159 8 PRRSV PRRSV NNP cord-275413-e2rhioty 159 9 - - HYPH cord-275413-e2rhioty 159 10 infected infect VBN cord-275413-e2rhioty 159 11 fetuses fetus NNS cord-275413-e2rhioty 159 12 appeared appear VBD cord-275413-e2rhioty 159 13 much much RB cord-275413-e2rhioty 159 14 more more RBR cord-275413-e2rhioty 159 15 pronounced pronounced JJ cord-275413-e2rhioty 159 16 and and CC cord-275413-e2rhioty 159 17 enlarged enlarged JJ cord-275413-e2rhioty 159 18 . . . cord-275413-e2rhioty 160 1 At at IN cord-275413-e2rhioty 160 2 the the DT cord-275413-e2rhioty 160 3 microscopic microscopic JJ cord-275413-e2rhioty 160 4 level level NN cord-275413-e2rhioty 160 5 , , , cord-275413-e2rhioty 160 6 the the DT cord-275413-e2rhioty 160 7 increased increase VBN cord-275413-e2rhioty 160 8 lymph lymph NN cord-275413-e2rhioty 160 9 node node NN cord-275413-e2rhioty 160 10 volume volume NN cord-275413-e2rhioty 160 11 was be VBD cord-275413-e2rhioty 160 12 associated associate VBN cord-275413-e2rhioty 160 13 with with IN cord-275413-e2rhioty 160 14 increased increase VBN cord-275413-e2rhioty 160 15 numbers number NNS cord-275413-e2rhioty 160 16 of of IN cord-275413-e2rhioty 160 17 cells cell NNS cord-275413-e2rhioty 160 18 and and CC cord-275413-e2rhioty 160 19 the the DT cord-275413-e2rhioty 160 20 formation formation NN cord-275413-e2rhioty 160 21 of of IN cord-275413-e2rhioty 160 22 distinct distinct JJ cord-275413-e2rhioty 160 23 germinal germinal JJ cord-275413-e2rhioty 160 24 centers center NNS cord-275413-e2rhioty 160 25 ( ( -LRB- cord-275413-e2rhioty 160 26 see see VB cord-275413-e2rhioty 160 27 Fig Fig NNP cord-275413-e2rhioty 160 28 . . NNP cord-275413-e2rhioty 160 29 2 2 CD cord-275413-e2rhioty 160 30 panels panel NNS cord-275413-e2rhioty 160 31 A a VBP cord-275413-e2rhioty 160 32 and and CC cord-275413-e2rhioty 160 33 _SP cord-275413-e2rhioty 160 34 B B NNP cord-275413-e2rhioty 160 35 ) ) -RRB- cord-275413-e2rhioty 160 36 . . . cord-275413-e2rhioty 161 1 The the DT cord-275413-e2rhioty 161 2 overall overall JJ cord-275413-e2rhioty 161 3 appearance appearance NN cord-275413-e2rhioty 161 4 is be VBZ cord-275413-e2rhioty 161 5 consistent consistent JJ cord-275413-e2rhioty 161 6 with with IN cord-275413-e2rhioty 161 7 an an DT cord-275413-e2rhioty 161 8 antigen antigen NN cord-275413-e2rhioty 161 9 - - HYPH cord-275413-e2rhioty 161 10 activated activate VBN cord-275413-e2rhioty 161 11 lymph lymph NN cord-275413-e2rhioty 161 12 node node NN cord-275413-e2rhioty 161 13 . . . cord-275413-e2rhioty 162 1 To to TO cord-275413-e2rhioty 162 2 determine determine VB cord-275413-e2rhioty 162 3 the the DT cord-275413-e2rhioty 162 4 source source NN cord-275413-e2rhioty 162 5 of of IN cord-275413-e2rhioty 162 6 the the DT cord-275413-e2rhioty 162 7 increased increase VBN cord-275413-e2rhioty 162 8 cell cell NN cord-275413-e2rhioty 162 9 volume volume NN cord-275413-e2rhioty 162 10 , , , cord-275413-e2rhioty 162 11 formalin formalin NN cord-275413-e2rhioty 162 12 - - HYPH cord-275413-e2rhioty 162 13 fixed fix VBN cord-275413-e2rhioty 162 14 thin thin JJ cord-275413-e2rhioty 162 15 sections section NNS cord-275413-e2rhioty 162 16 of of IN cord-275413-e2rhioty 162 17 lymph lymph NN cord-275413-e2rhioty 162 18 nodes node NNS cord-275413-e2rhioty 162 19 were be VBD cord-275413-e2rhioty 162 20 stained stain VBN cord-275413-e2rhioty 162 21 with with IN cord-275413-e2rhioty 162 22 T T NNP cord-275413-e2rhioty 162 23 cell cell NN cord-275413-e2rhioty 162 24 - - HYPH cord-275413-e2rhioty 162 25 specific specific JJ cord-275413-e2rhioty 162 26 ( ( -LRB- cord-275413-e2rhioty 162 27 CD3 CD3 NNP cord-275413-e2rhioty 162 28 ) ) -RRB- cord-275413-e2rhioty 162 29 and and CC cord-275413-e2rhioty 162 30 B b NN cord-275413-e2rhioty 162 31 cell cell NN cord-275413-e2rhioty 162 32 - - HYPH cord-275413-e2rhioty 162 33 specific specific JJ cord-275413-e2rhioty 162 34 ( ( -LRB- cord-275413-e2rhioty 162 35 CDw75 CDw75 NNS cord-275413-e2rhioty 162 36 and and CC cord-275413-e2rhioty 162 37 CD79 CD79 NNP cord-275413-e2rhioty 162 38 ␣ ␣ NNP cord-275413-e2rhioty 162 39 ) ) -RRB- cord-275413-e2rhioty 162 40 antibodies antibody NNS cord-275413-e2rhioty 162 41 . . . cord-275413-e2rhioty 163 1 Representative representative JJ cord-275413-e2rhioty 163 2 results result NNS cord-275413-e2rhioty 163 3 for for IN cord-275413-e2rhioty 163 4 mandibular mandibular JJ cord-275413-e2rhioty 163 5 lymph lymph NN cord-275413-e2rhioty 163 6 nodes node NNS cord-275413-e2rhioty 163 7 from from IN cord-275413-e2rhioty 163 8 infected infected JJ cord-275413-e2rhioty 163 9 and and CC cord-275413-e2rhioty 163 10 non non JJ cord-275413-e2rhioty 163 11 - - JJ cord-275413-e2rhioty 163 12 infected infected JJ cord-275413-e2rhioty 163 13 fetuses fetus NNS cord-275413-e2rhioty 163 14 are be VBP cord-275413-e2rhioty 163 15 shown show VBN cord-275413-e2rhioty 163 16 in in IN cord-275413-e2rhioty 163 17 Fig Fig NNP cord-275413-e2rhioty 163 18 . . . cord-275413-e2rhioty 164 1 3 3 LS cord-275413-e2rhioty 164 2 . . . cord-275413-e2rhioty 165 1 The the DT cord-275413-e2rhioty 165 2 lymph lymph NN cord-275413-e2rhioty 165 3 nodes node NNS cord-275413-e2rhioty 165 4 from from IN cord-275413-e2rhioty 165 5 both both CC cord-275413-e2rhioty 165 6 infected infected JJ cord-275413-e2rhioty 165 7 and and CC cord-275413-e2rhioty 165 8 non non JJ cord-275413-e2rhioty 165 9 - - JJ cord-275413-e2rhioty 165 10 infected infected JJ cord-275413-e2rhioty 165 11 fetuses fetus NNS cord-275413-e2rhioty 165 12 showed show VBD cord-275413-e2rhioty 165 13 exten- exten- JJ cord-275413-e2rhioty 165 14 _SP cord-275413-e2rhioty 165 15 sive sive JJ cord-275413-e2rhioty 165 16 areas area NNS cord-275413-e2rhioty 165 17 of of IN cord-275413-e2rhioty 165 18 staining stain VBG cord-275413-e2rhioty 165 19 with with IN cord-275413-e2rhioty 165 20 anti anti JJ cord-275413-e2rhioty 165 21 - - JJ cord-275413-e2rhioty 165 22 CD3 CD3 NNP cord-275413-e2rhioty 165 23 , , , cord-275413-e2rhioty 165 24 indicating indicate VBG cord-275413-e2rhioty 165 25 the the DT cord-275413-e2rhioty 165 26 presence presence NN cord-275413-e2rhioty 165 27 of of IN cord-275413-e2rhioty 165 28 T t NN cord-275413-e2rhioty 165 29 cells cell NNS cord-275413-e2rhioty 165 30 . . . cord-275413-e2rhioty 166 1 Lymph lymph NN cord-275413-e2rhioty 166 2 nodes node NNS cord-275413-e2rhioty 166 3 from from IN cord-275413-e2rhioty 166 4 non non JJ cord-275413-e2rhioty 166 5 - - JJ cord-275413-e2rhioty 166 6 infected infected JJ cord-275413-e2rhioty 166 7 fetuses fetus NNS cord-275413-e2rhioty 166 8 were be VBD cord-275413-e2rhioty 166 9 negative negative JJ cord-275413-e2rhioty 166 10 for for IN cord-275413-e2rhioty 166 11 CwD75 CwD75 NNP cord-275413-e2rhioty 166 12 staining staining NN cord-275413-e2rhioty 166 13 , , , cord-275413-e2rhioty 166 14 but but CC cord-275413-e2rhioty 166 15 possessed possess VBD cord-275413-e2rhioty 166 16 some some DT cord-275413-e2rhioty 166 17 regions region NNS cord-275413-e2rhioty 166 18 that that WDT cord-275413-e2rhioty 166 19 were be VBD cord-275413-e2rhioty 166 20 positive positive JJ cord-275413-e2rhioty 166 21 for for IN cord-275413-e2rhioty 166 22 CD79 CD79 NNP cord-275413-e2rhioty 167 1 ␣ ␣ NNP cord-275413-e2rhioty 167 2 . . . cord-275413-e2rhioty 168 1 The the DT cord-275413-e2rhioty 168 2 B b NN cord-275413-e2rhioty 168 3 cell cell NN cord-275413-e2rhioty 168 4 receptor receptor NN cord-275413-e2rhioty 168 5 for for IN cord-275413-e2rhioty 168 6 antigen antigen NN cord-275413-e2rhioty 168 7 ( ( -LRB- cord-275413-e2rhioty 168 8 BCR BCR NNP cord-275413-e2rhioty 168 9 ) ) -RRB- cord-275413-e2rhioty 168 10 signal signal NN cord-275413-e2rhioty 168 11 transduction transduction NN cord-275413-e2rhioty 168 12 complex complex NN cord-275413-e2rhioty 168 13 is be VBZ cord-275413-e2rhioty 168 14 composed compose VBN cord-275413-e2rhioty 168 15 of of IN cord-275413-e2rhioty 168 16 a a DT cord-275413-e2rhioty 168 17 heterodimer heterodimer NN cord-275413-e2rhioty 168 18 of of IN cord-275413-e2rhioty 168 19 Ig Ig NNP cord-275413-e2rhioty 168 20 ␣ ␣ NNP cord-275413-e2rhioty 168 21 and and CC cord-275413-e2rhioty 168 22 Ig Ig NNP cord-275413-e2rhioty 168 23 ␤ ␤ NNP cord-275413-e2rhioty 168 24 chains chain NNS cord-275413-e2rhioty 168 25 , , , cord-275413-e2rhioty 168 26 which which WDT cord-275413-e2rhioty 168 27 are be VBP cord-275413-e2rhioty 168 28 also also RB cord-275413-e2rhioty 168 29 known know VBN cord-275413-e2rhioty 168 30 as as IN cord-275413-e2rhioty 168 31 CD79 CD79 NNP cord-275413-e2rhioty 169 1 ␣ ␣ NNP cord-275413-e2rhioty 169 2 and and CC cord-275413-e2rhioty 169 3 CD79 CD79 NNP cord-275413-e2rhioty 169 4 ␤ ␤ NNP cord-275413-e2rhioty 169 5 , , , cord-275413-e2rhioty 169 6 respectively respectively RB cord-275413-e2rhioty 169 7 . . . cord-275413-e2rhioty 170 1 CD79 CD79 NNP cord-275413-e2rhioty 171 1 ␣ ␣ LS cord-275413-e2rhioty 171 2 staining stain VBG cord-275413-e2rhioty 171 3 in in IN cord-275413-e2rhioty 171 4 the the DT cord-275413-e2rhioty 171 5 lymph lymph NN cord-275413-e2rhioty 171 6 node node NN cord-275413-e2rhioty 171 7 from from IN cord-275413-e2rhioty 171 8 non non JJ cord-275413-e2rhioty 171 9 - - JJ cord-275413-e2rhioty 171 10 infected infected JJ cord-275413-e2rhioty 171 11 fetuses fetus NNS cord-275413-e2rhioty 171 12 is be VBZ cord-275413-e2rhioty 171 13 consistent consistent JJ cord-275413-e2rhioty 171 14 with with IN cord-275413-e2rhioty 171 15 the the DT cord-275413-e2rhioty 171 16 presence presence NN cord-275413-e2rhioty 171 17 of of IN cord-275413-e2rhioty 171 18 pro pro JJ cord-275413-e2rhioty 171 19 - - JJ cord-275413-e2rhioty 171 20 and and CC cord-275413-e2rhioty 171 21 pre pre JJ cord-275413-e2rhioty 171 22 - - JJ cord-275413-e2rhioty 171 23 B b NN cord-275413-e2rhioty 171 24 cells cell NNS cord-275413-e2rhioty 171 25 ( ( -LRB- cord-275413-e2rhioty 171 26 Lee Lee NNP cord-275413-e2rhioty 171 27 et et NNP cord-275413-e2rhioty 171 28 al al NNP cord-275413-e2rhioty 171 29 . . NNP cord-275413-e2rhioty 171 30 , , , cord-275413-e2rhioty 171 31 2008 2008 CD cord-275413-e2rhioty 171 32 ) ) -RRB- cord-275413-e2rhioty 171 33 . . . cord-275413-e2rhioty 172 1 The the DT cord-275413-e2rhioty 172 2 principle principle JJ cord-275413-e2rhioty 172 3 difference difference NN cord-275413-e2rhioty 172 4 between between IN cord-275413-e2rhioty 172 5 infected infected JJ cord-275413-e2rhioty 172 6 and and CC cord-275413-e2rhioty 172 7 non non JJ cord-275413-e2rhioty 172 8 - - JJ cord-275413-e2rhioty 172 9 infected infected JJ cord-275413-e2rhioty 172 10 fetuses fetus NNS cord-275413-e2rhioty 172 11 was be VBD cord-275413-e2rhioty 172 12 found find VBN cord-275413-e2rhioty 172 13 in in IN cord-275413-e2rhioty 172 14 an an DT cord-275413-e2rhioty 172 15 overall overall JJ cord-275413-e2rhioty 172 16 increase increase NN cord-275413-e2rhioty 172 17 in in IN cord-275413-e2rhioty 172 18 CD79 CD79 NNP cord-275413-e2rhioty 173 1 ␣ ␣ NNP cord-275413-e2rhioty 173 2 + + CC cord-275413-e2rhioty 173 3 cells cell NNS cord-275413-e2rhioty 173 4 as as RB cord-275413-e2rhioty 173 5 well well RB cord-275413-e2rhioty 173 6 as as IN cord-275413-e2rhioty 173 7 the the DT cord-275413-e2rhioty 173 8 appearance appearance NN cord-275413-e2rhioty 173 9 of of IN cord-275413-e2rhioty 173 10 CDw75 CDw75 NNS cord-275413-e2rhioty 173 11 + + CC cord-275413-e2rhioty 173 12 cells cell NNS cord-275413-e2rhioty 173 13 , , , cord-275413-e2rhioty 173 14 which which WDT cord-275413-e2rhioty 173 15 were be VBD cord-275413-e2rhioty 173 16 associated associate VBN cord-275413-e2rhioty 173 17 with with IN cord-275413-e2rhioty 173 18 germinal germinal JJ cord-275413-e2rhioty 173 19 centers center NNS cord-275413-e2rhioty 173 20 . . . cord-275413-e2rhioty 174 1 CDw79 CDw79 NNP cord-275413-e2rhioty 174 2 is be VBZ cord-275413-e2rhioty 174 3 beta beta NN cord-275413-e2rhioty 174 4 - - HYPH cord-275413-e2rhioty 174 5 galactoside galactoside JJ cord-275413-e2rhioty 174 6 alpha-2,6-sialyltransferase alpha-2,6-sialyltransferase NNP cord-275413-e2rhioty 174 7 , , , cord-275413-e2rhioty 174 8 which which WDT cord-275413-e2rhioty 174 9 is be VBZ cord-275413-e2rhioty 174 10 up up RB cord-275413-e2rhioty 174 11 - - HYPH cord-275413-e2rhioty 174 12 regulated regulate VBN cord-275413-e2rhioty 174 13 in in IN cord-275413-e2rhioty 174 14 activated activate VBN cord-275413-e2rhioty 174 15 B b NN cord-275413-e2rhioty 174 16 cells cell NNS cord-275413-e2rhioty 175 1 ( ( -LRB- cord-275413-e2rhioty 175 2 Erikstein Erikstein NNP cord-275413-e2rhioty 175 3 et et NNP cord-275413-e2rhioty 175 4 al al NNP cord-275413-e2rhioty 175 5 . . NNP cord-275413-e2rhioty 175 6 , , , cord-275413-e2rhioty 175 7 1992 1992 CD cord-275413-e2rhioty 175 8 ) ) -RRB- cord-275413-e2rhioty 175 9 . . . cord-275413-e2rhioty 176 1 The the DT cord-275413-e2rhioty 176 2 absence absence NN cord-275413-e2rhioty 176 3 of of IN cord-275413-e2rhioty 176 4 CDw75 CDw75 NNS cord-275413-e2rhioty 176 5 staining staining NN cord-275413-e2rhioty 176 6 in in IN cord-275413-e2rhioty 176 7 non non JJ cord-275413-e2rhioty 176 8 - - JJ cord-275413-e2rhioty 176 9 infected infected JJ cord-275413-e2rhioty 176 10 fetuses fetus NNS cord-275413-e2rhioty 176 11 is be VBZ cord-275413-e2rhioty 176 12 consistent consistent JJ cord-275413-e2rhioty 176 13 with with IN cord-275413-e2rhioty 176 14 the the DT cord-275413-e2rhioty 176 15 overall overall JJ cord-275413-e2rhioty 176 16 quiescent quiescent JJ cord-275413-e2rhioty 176 17 nature nature NN cord-275413-e2rhioty 176 18 of of IN cord-275413-e2rhioty 176 19 the the DT cord-275413-e2rhioty 176 20 non non JJ cord-275413-e2rhioty 176 21 - - JJ cord-275413-e2rhioty 176 22 stimulated stimulated JJ cord-275413-e2rhioty 176 23 fetal fetal JJ cord-275413-e2rhioty 176 24 immune immune JJ cord-275413-e2rhioty 176 25 system system NN cord-275413-e2rhioty 176 26 . . . cord-275413-e2rhioty 177 1 The the DT cord-275413-e2rhioty 177 2 up up JJ cord-275413-e2rhioty 177 3 - - HYPH cord-275413-e2rhioty 177 4 regulation regulation NN cord-275413-e2rhioty 177 5 of of IN cord-275413-e2rhioty 177 6 CDw75 CDw75 NNS cord-275413-e2rhioty 177 7 after after IN cord-275413-e2rhioty 177 8 PRRSV PRRSV NNP cord-275413-e2rhioty 177 9 infection infection NN cord-275413-e2rhioty 177 10 is be VBZ cord-275413-e2rhioty 177 11 consistent consistent JJ cord-275413-e2rhioty 177 12 with with IN cord-275413-e2rhioty 177 13 B b NN cord-275413-e2rhioty 177 14 cell cell NN cord-275413-e2rhioty 177 15 activation activation NN cord-275413-e2rhioty 177 16 and and CC cord-275413-e2rhioty 177 17 the the DT cord-275413-e2rhioty 177 18 formation formation NN cord-275413-e2rhioty 177 19 of of IN cord-275413-e2rhioty 177 20 mature mature JJ cord-275413-e2rhioty 177 21 germinal germinal JJ cord-275413-e2rhioty 177 22 centers center NNS cord-275413-e2rhioty 177 23 in in IN cord-275413-e2rhioty 177 24 response response NN cord-275413-e2rhioty 177 25 to to IN cord-275413-e2rhioty 177 26 infection infection NN cord-275413-e2rhioty 177 27 . . . cord-275413-e2rhioty 178 1 It -PRON- PRP cord-275413-e2rhioty 178 2 should should MD cord-275413-e2rhioty 178 3 be be VB cord-275413-e2rhioty 178 4 noted note VBN cord-275413-e2rhioty 178 5 that that IN cord-275413-e2rhioty 178 6 infected infected JJ cord-275413-e2rhioty 178 7 fetuses fetus NNS cord-275413-e2rhioty 178 8 showed show VBD cord-275413-e2rhioty 178 9 different different JJ cord-275413-e2rhioty 178 10 degrees degree NNS cord-275413-e2rhioty 178 11 of of IN cord-275413-e2rhioty 178 12 staining staining NN cord-275413-e2rhioty 178 13 , , , cord-275413-e2rhioty 178 14 a a DT cord-275413-e2rhioty 178 15 likely likely JJ cord-275413-e2rhioty 178 16 consequence consequence NN cord-275413-e2rhioty 178 17 of of IN cord-275413-e2rhioty 178 18 the the DT cord-275413-e2rhioty 178 19 different different JJ cord-275413-e2rhioty 178 20 stages stage NNS cord-275413-e2rhioty 178 21 of of IN cord-275413-e2rhioty 178 22 fetal fetal JJ cord-275413-e2rhioty 178 23 infection infection NN cord-275413-e2rhioty 178 24 ; ; : cord-275413-e2rhioty 178 25 i.e. i.e. FW cord-275413-e2rhioty 178 26 less less RBR cord-275413-e2rhioty 178 27 staining staining NN cord-275413-e2rhioty 178 28 was be VBD cord-275413-e2rhioty 178 29 the the DT cord-275413-e2rhioty 178 30 result result NN cord-275413-e2rhioty 178 31 of of IN cord-275413-e2rhioty 178 32 early early JJ cord-275413-e2rhioty 178 33 infection infection NN cord-275413-e2rhioty 178 34 . . . cord-275413-e2rhioty 179 1 Together together RB cord-275413-e2rhioty 179 2 , , , cord-275413-e2rhioty 179 3 the the DT cord-275413-e2rhioty 179 4 overall overall JJ cord-275413-e2rhioty 179 5 morphology morphology NN cord-275413-e2rhioty 179 6 and and CC cord-275413-e2rhioty 179 7 lymphocyte lymphocyte NN cord-275413-e2rhioty 179 8 marker marker NN cord-275413-e2rhioty 179 9 expression expression NN cord-275413-e2rhioty 179 10 results result NNS cord-275413-e2rhioty 179 11 indicate indicate VBP cord-275413-e2rhioty 179 12 that that IN cord-275413-e2rhioty 179 13 the the DT cord-275413-e2rhioty 179 14 increased increase VBN cord-275413-e2rhioty 179 15 volume volume NN cord-275413-e2rhioty 179 16 in in IN cord-275413-e2rhioty 179 17 the the DT cord-275413-e2rhioty 179 18 lymph lymph NN cord-275413-e2rhioty 179 19 nodes node NNS cord-275413-e2rhioty 179 20 of of IN cord-275413-e2rhioty 179 21 infected infected JJ cord-275413-e2rhioty 179 22 fetuses fetus NNS cord-275413-e2rhioty 179 23 is be VBZ cord-275413-e2rhioty 179 24 largely largely RB cord-275413-e2rhioty 179 25 the the DT cord-275413-e2rhioty 179 26 result result NN cord-275413-e2rhioty 179 27 of of IN cord-275413-e2rhioty 179 28 increased increase VBN cord-275413-e2rhioty 179 29 numbers number NNS cord-275413-e2rhioty 179 30 of of IN cord-275413-e2rhioty 179 31 mature mature JJ cord-275413-e2rhioty 179 32 activated activate VBN cord-275413-e2rhioty 179 33 B b NN cord-275413-e2rhioty 179 34 cells cell NNS cord-275413-e2rhioty 179 35 , , , cord-275413-e2rhioty 179 36 which which WDT cord-275413-e2rhioty 179 37 occupy occupy VBP cord-275413-e2rhioty 179 38 germinal germinal JJ cord-275413-e2rhioty 179 39 centers center NNS cord-275413-e2rhioty 179 40 . . . cord-275413-e2rhioty 180 1 Immune immune JJ cord-275413-e2rhioty 180 2 cytokines cytokine NNS cord-275413-e2rhioty 180 3 are be VBP cord-275413-e2rhioty 180 4 important important JJ cord-275413-e2rhioty 180 5 factors factor NNS cord-275413-e2rhioty 180 6 in in IN cord-275413-e2rhioty 180 7 antiviral antiviral JJ cord-275413-e2rhioty 180 8 immunity immunity NN cord-275413-e2rhioty 180 9 and and CC cord-275413-e2rhioty 180 10 can can MD cord-275413-e2rhioty 180 11 influence influence VB cord-275413-e2rhioty 180 12 the the DT cord-275413-e2rhioty 180 13 outcome outcome NN cord-275413-e2rhioty 180 14 of of IN cord-275413-e2rhioty 180 15 pregnancy pregnancy NN cord-275413-e2rhioty 181 1 ( ( -LRB- cord-275413-e2rhioty 181 2 Arck Arck NNP cord-275413-e2rhioty 181 3 et et FW cord-275413-e2rhioty 181 4 al al NNP cord-275413-e2rhioty 181 5 . . NNP cord-275413-e2rhioty 181 6 , , , cord-275413-e2rhioty 181 7 1999 1999 CD cord-275413-e2rhioty 181 8 ; ; : cord-275413-e2rhioty 181 9 Basurko Basurko NNP cord-275413-e2rhioty 181 10 et et NNP cord-275413-e2rhioty 181 11 al al NNP cord-275413-e2rhioty 181 12 . . NNP cord-275413-e2rhioty 181 13 , , , cord-275413-e2rhioty 181 14 2009 2009 CD cord-275413-e2rhioty 181 15 ) ) -RRB- cord-275413-e2rhioty 181 16 . . . cord-275413-e2rhioty 182 1 The the DT cord-275413-e2rhioty 182 2 detection detection NN cord-275413-e2rhioty 182 3 of of IN cord-275413-e2rhioty 182 4 cytokine cytokine NN cord-275413-e2rhioty 182 5 gene gene NN cord-275413-e2rhioty 182 6 expression expression NN cord-275413-e2rhioty 182 7 in in IN cord-275413-e2rhioty 182 8 tissues tissue NNS cord-275413-e2rhioty 182 9 was be VBD cord-275413-e2rhioty 182 10 performed perform VBN cord-275413-e2rhioty 182 11 using use VBG cord-275413-e2rhioty 182 12 a a DT cord-275413-e2rhioty 182 13 steady steady JJ cord-275413-e2rhioty 182 14 state state NN cord-275413-e2rhioty 182 15 RT RT NNP cord-275413-e2rhioty 182 16 - - HYPH cord-275413-e2rhioty 182 17 PCR PCR NNP cord-275413-e2rhioty 182 18 procedure procedure NN cord-275413-e2rhioty 182 19 . . . cord-275413-e2rhioty 183 1 RT RT NNP cord-275413-e2rhioty 183 2 - - HYPH cord-275413-e2rhioty 183 3 PCR PCR NNP cord-275413-e2rhioty 183 4 was be VBD cord-275413-e2rhioty 183 5 performed perform VBN cord-275413-e2rhioty 183 6 on on IN cord-275413-e2rhioty 183 7 RNA RNA NNP cord-275413-e2rhioty 183 8 isolated isolate VBN cord-275413-e2rhioty 183 9 from from IN cord-275413-e2rhioty 183 10 four four CD cord-275413-e2rhioty 183 11 fetuses fetus NNS cord-275413-e2rhioty 183 12 randomly randomly RB cord-275413-e2rhioty 183 13 chosen choose VBN cord-275413-e2rhioty 183 14 from from IN cord-275413-e2rhioty 183 15 the the DT cord-275413-e2rhioty 183 16 two two CD cord-275413-e2rhioty 183 17 mock mock JJ cord-275413-e2rhioty 183 18 - - HYPH cord-275413-e2rhioty 183 19 infected infect VBN cord-275413-e2rhioty 183 20 dams dam NNS cord-275413-e2rhioty 183 21 and and CC cord-275413-e2rhioty 183 22 four four CD cord-275413-e2rhioty 183 23 randomly randomly RB cord-275413-e2rhioty 183 24 selected select VBN cord-275413-e2rhioty 183 25 PRRSV PRRSV NNP cord-275413-e2rhioty 183 26 - - HYPH cord-275413-e2rhioty 183 27 infected infect VBN cord-275413-e2rhioty 183 28 fetuses fetus NNS cord-275413-e2rhioty 183 29 . . . cord-275413-e2rhioty 184 1 The the DT cord-275413-e2rhioty 184 2 tissues tissue NNS cord-275413-e2rhioty 184 3 selected select VBN cord-275413-e2rhioty 184 4 for for IN cord-275413-e2rhioty 184 5 RT RT NNP cord-275413-e2rhioty 184 6 - - HYPH cord-275413-e2rhioty 184 7 PCR PCR NNP cord-275413-e2rhioty 184 8 amplification amplification NN cord-275413-e2rhioty 184 9 were be VBD cord-275413-e2rhioty 184 10 lung lung NN cord-275413-e2rhioty 184 11 , , , cord-275413-e2rhioty 184 12 lymph lymph NN cord-275413-e2rhioty 184 13 node node NN cord-275413-e2rhioty 184 14 and and CC cord-275413-e2rhioty 184 15 placenta placenta NN cord-275413-e2rhioty 184 16 . . . cord-275413-e2rhioty 185 1 Lung lung NN cord-275413-e2rhioty 185 2 and and CC cord-275413-e2rhioty 185 3 lymph lymph NN cord-275413-e2rhioty 185 4 node node NN cord-275413-e2rhioty 185 5 represent represent VBP cord-275413-e2rhioty 185 6 sites site NNS cord-275413-e2rhioty 185 7 cytokine cytokine JJ cord-275413-e2rhioty 185 8 alterations alteration NNS cord-275413-e2rhioty 185 9 in in IN cord-275413-e2rhioty 185 10 the the DT cord-275413-e2rhioty 185 11 post post JJ cord-275413-e2rhioty 185 12 - - JJ cord-275413-e2rhioty 185 13 natal natal JJ cord-275413-e2rhioty 185 14 pig pig NN cord-275413-e2rhioty 185 15 . . . cord-275413-e2rhioty 186 1 Placenta placenta NN cord-275413-e2rhioty 186 2 was be VBD cord-275413-e2rhioty 186 3 selected select VBN cord-275413-e2rhioty 186 4 as as IN cord-275413-e2rhioty 186 5 an an DT cord-275413-e2rhioty 186 6 accessory accessory NN cord-275413-e2rhioty 186 7 tissue tissue NN cord-275413-e2rhioty 186 8 located locate VBN cord-275413-e2rhioty 186 9 at at IN cord-275413-e2rhioty 186 10 the the DT cord-275413-e2rhioty 186 11 fetal fetal JJ cord-275413-e2rhioty 186 12 maternal maternal JJ cord-275413-e2rhioty 186 13 interface interface NN cord-275413-e2rhioty 186 14 . . . cord-275413-e2rhioty 187 1 Amplification amplification NN cord-275413-e2rhioty 187 2 of of IN cord-275413-e2rhioty 187 3 mRNAs mRNAs NNPS cord-275413-e2rhioty 187 4 included include VBD cord-275413-e2rhioty 187 5 cytokines cytokine NNS cord-275413-e2rhioty 187 6 associated associate VBN cord-275413-e2rhioty 187 7 with with IN cord-275413-e2rhioty 187 8 inflammatory inflammatory JJ cord-275413-e2rhioty 187 9 ( ( -LRB- cord-275413-e2rhioty 187 10 IL-6 IL-6 NNP cord-275413-e2rhioty 187 11 , , , cord-275413-e2rhioty 187 12 IL-8 IL-8 NNP cord-275413-e2rhioty 187 13 ) ) -RRB- cord-275413-e2rhioty 187 14 , , , cord-275413-e2rhioty 187 15 Th1 th1 NN cord-275413-e2rhioty 187 16 ( ( -LRB- cord-275413-e2rhioty 187 17 IL-12 IL-12 NNP cord-275413-e2rhioty 187 18 , , , cord-275413-e2rhioty 187 19 IFN- IFN- NNP cord-275413-e2rhioty 187 20 ␥ ␥ NNP cord-275413-e2rhioty 187 21 , , , cord-275413-e2rhioty 187 22 IL-2 IL-2 NNP cord-275413-e2rhioty 187 23 ) ) -RRB- cord-275413-e2rhioty 187 24 and and CC cord-275413-e2rhioty 187 25 Th2 Th2 NNP cord-275413-e2rhioty 187 26 / / SYM cord-275413-e2rhioty 187 27 regulatory regulatory JJ cord-275413-e2rhioty 187 28 ( ( -LRB- cord-275413-e2rhioty 187 29 IL-4 IL-4 NNP cord-275413-e2rhioty 187 30 , , , cord-275413-e2rhioty 187 31 IL-10 IL-10 NNP cord-275413-e2rhioty 187 32 ) ) -RRB- cord-275413-e2rhioty 187 33 responses response NNS cord-275413-e2rhioty 187 34 . . . cord-275413-e2rhioty 188 1 The the DT cord-275413-e2rhioty 188 2 amplification amplification NN cord-275413-e2rhioty 188 3 of of IN cord-275413-e2rhioty 188 4 ␤ ␤ CD cord-275413-e2rhioty 188 5 2 2 CD cord-275413-e2rhioty 188 6 m m NNP cord-275413-e2rhioty 188 7 mRNA mRNA NNP cord-275413-e2rhioty 188 8 was be VBD cord-275413-e2rhioty 188 9 included include VBN cord-275413-e2rhioty 188 10 as as IN cord-275413-e2rhioty 188 11 an an DT cord-275413-e2rhioty 188 12 internal internal JJ cord-275413-e2rhioty 188 13 control control NN cord-275413-e2rhioty 188 14 . . . cord-275413-e2rhioty 189 1 The the DT cord-275413-e2rhioty 189 2 determination determination NN cord-275413-e2rhioty 189 3 of of IN cord-275413-e2rhioty 189 4 a a DT cord-275413-e2rhioty 189 5 cytokine cytokine NN cord-275413-e2rhioty 189 6 response response NN cord-275413-e2rhioty 189 7 was be VBD cord-275413-e2rhioty 189 8 based base VBN cord-275413-e2rhioty 189 9 on on IN cord-275413-e2rhioty 189 10 the the DT cord-275413-e2rhioty 189 11 presence presence NN cord-275413-e2rhioty 189 12 or or CC cord-275413-e2rhioty 189 13 absence absence NN cord-275413-e2rhioty 189 14 of of IN cord-275413-e2rhioty 189 15 a a DT cord-275413-e2rhioty 189 16 PCR PCR NNP cord-275413-e2rhioty 189 17 product product NN cord-275413-e2rhioty 189 18 . . . cord-275413-e2rhioty 190 1 IL-6 IL-6 NNP cord-275413-e2rhioty 190 2 , , , cord-275413-e2rhioty 190 3 IL-8 IL-8 NNP cord-275413-e2rhioty 190 4 , , , cord-275413-e2rhioty 190 5 IL-12 IL-12 NNP cord-275413-e2rhioty 190 6 and and CC cord-275413-e2rhioty 190 7 IL-10 IL-10 NNP cord-275413-e2rhioty 190 8 products product NNS cord-275413-e2rhioty 190 9 were be VBD cord-275413-e2rhioty 190 10 detected detect VBN cord-275413-e2rhioty 190 11 in in IN cord-275413-e2rhioty 190 12 tissues tissue NNS cord-275413-e2rhioty 190 13 from from IN cord-275413-e2rhioty 190 14 both both DT cord-275413-e2rhioty 190 15 control control NN cord-275413-e2rhioty 190 16 and and CC cord-275413-e2rhioty 190 17 infected infected JJ cord-275413-e2rhioty 190 18 fetuses fetus NNS cord-275413-e2rhioty 190 19 . . . cord-275413-e2rhioty 191 1 Because because IN cord-275413-e2rhioty 191 2 of of IN cord-275413-e2rhioty 191 3 the the DT cord-275413-e2rhioty 191 4 qualitative qualitative JJ cord-275413-e2rhioty 191 5 nature nature NN cord-275413-e2rhioty 191 6 of of IN cord-275413-e2rhioty 191 7 the the DT cord-275413-e2rhioty 191 8 PCR PCR NNP cord-275413-e2rhioty 191 9 method method NN cord-275413-e2rhioty 191 10 , , , cord-275413-e2rhioty 191 11 it -PRON- PRP cord-275413-e2rhioty 191 12 was be VBD cord-275413-e2rhioty 191 13 not not RB cord-275413-e2rhioty 191 14 possible possible JJ cord-275413-e2rhioty 191 15 to to TO cord-275413-e2rhioty 191 16 accurately accurately RB cord-275413-e2rhioty 191 17 determine determine VB cord-275413-e2rhioty 191 18 quantitative quantitative JJ cord-275413-e2rhioty 191 19 differences difference NNS cord-275413-e2rhioty 191 20 between between IN cord-275413-e2rhioty 191 21 control control NN cord-275413-e2rhioty 191 22 and and CC cord-275413-e2rhioty 191 23 infected infected JJ cord-275413-e2rhioty 191 24 fetuses fetus NNS cord-275413-e2rhioty 191 25 ; ; : cord-275413-e2rhioty 191 26 and and CC cord-275413-e2rhioty 191 27 therefore therefore RB cord-275413-e2rhioty 191 28 , , , cord-275413-e2rhioty 191 29 these these DT cord-275413-e2rhioty 191 30 cytokines cytokine NNS cord-275413-e2rhioty 191 31 were be VBD cord-275413-e2rhioty 191 32 not not RB cord-275413-e2rhioty 191 33 subjected subject VBN cord-275413-e2rhioty 191 34 to to TO cord-275413-e2rhioty 191 35 further further JJ cord-275413-e2rhioty 191 36 study study VB cord-275413-e2rhioty 191 37 ( ( -LRB- cord-275413-e2rhioty 191 38 data datum NNS cord-275413-e2rhioty 191 39 not not RB cord-275413-e2rhioty 191 40 shown show VBN cord-275413-e2rhioty 191 41 ) ) -RRB- cord-275413-e2rhioty 191 42 . . . cord-275413-e2rhioty 192 1 IL-4 IL-4 NNP cord-275413-e2rhioty 193 1 mRNA mRNA NNP cord-275413-e2rhioty 193 2 was be VBD cord-275413-e2rhioty 193 3 not not RB cord-275413-e2rhioty 193 4 detected detect VBN cord-275413-e2rhioty 193 5 in in IN cord-275413-e2rhioty 193 6 any any DT cord-275413-e2rhioty 193 7 of of IN cord-275413-e2rhioty 193 8 the the DT cord-275413-e2rhioty 193 9 selected select VBN cord-275413-e2rhioty 193 10 tissues tissue NNS cord-275413-e2rhioty 193 11 for for IN cord-275413-e2rhioty 193 12 control control NN cord-275413-e2rhioty 193 13 and and CC cord-275413-e2rhioty 193 14 infected infected JJ cord-275413-e2rhioty 193 15 fetuses fetus NNS cord-275413-e2rhioty 193 16 . . . cord-275413-e2rhioty 194 1 Marked marked JJ cord-275413-e2rhioty 194 2 differences difference NNS cord-275413-e2rhioty 194 3 in in IN cord-275413-e2rhioty 194 4 expression expression NN cord-275413-e2rhioty 194 5 were be VBD cord-275413-e2rhioty 194 6 observed observe VBN cord-275413-e2rhioty 194 7 for for IN cord-275413-e2rhioty 194 8 TNF- TNF- NNP cord-275413-e2rhioty 194 9 ␣ ␣ NNP cord-275413-e2rhioty 194 10 and and CC cord-275413-e2rhioty 194 11 IFN- IFN- NNP cord-275413-e2rhioty 194 12 ␥ ␥ NNP cord-275413-e2rhioty 194 13 mRNAs mrnas ADD cord-275413-e2rhioty 194 14 . . . cord-275413-e2rhioty 195 1 The the DT cord-275413-e2rhioty 195 2 results result NNS cord-275413-e2rhioty 195 3 for for IN cord-275413-e2rhioty 196 1 IFN- IFN- NNP cord-275413-e2rhioty 196 2 ␥ ␥ CD cord-275413-e2rhioty 196 3 and and CC cord-275413-e2rhioty 196 4 TNF- TNF- NNP cord-275413-e2rhioty 196 5 ␣ ␣ NN cord-275413-e2rhioty 196 6 from from IN cord-275413-e2rhioty 196 7 lung lung NN cord-275413-e2rhioty 196 8 , , , cord-275413-e2rhioty 196 9 mandibular mandibular JJ cord-275413-e2rhioty 196 10 lymph lymph NN cord-275413-e2rhioty 196 11 node node NN cord-275413-e2rhioty 196 12 and and CC cord-275413-e2rhioty 196 13 placenta placenta NN cord-275413-e2rhioty 196 14 are be VBP cord-275413-e2rhioty 196 15 presented present VBN cord-275413-e2rhioty 196 16 in in IN cord-275413-e2rhioty 196 17 Fig Fig NNP cord-275413-e2rhioty 196 18 . . . cord-275413-e2rhioty 197 1 4 4 LS cord-275413-e2rhioty 197 2 . . . cord-275413-e2rhioty 198 1 The the DT cord-275413-e2rhioty 198 2 results result NNS cord-275413-e2rhioty 198 3 for for IN cord-275413-e2rhioty 198 4 IL-10 IL-10 NNP cord-275413-e2rhioty 198 5 are be VBP cord-275413-e2rhioty 198 6 also also RB cord-275413-e2rhioty 198 7 shown show VBN cord-275413-e2rhioty 198 8 . . . cord-275413-e2rhioty 199 1 The the DT cord-275413-e2rhioty 199 2 internal internal JJ cord-275413-e2rhioty 199 3 control control NN cord-275413-e2rhioty 199 4 mRNA mrna NN cord-275413-e2rhioty 199 5 , , , cord-275413-e2rhioty 199 6 ␤ ␤ NNP cord-275413-e2rhioty 199 7 2 2 CD cord-275413-e2rhioty 199 8 m m NNP cord-275413-e2rhioty 199 9 , , , cord-275413-e2rhioty 199 10 was be VBD cord-275413-e2rhioty 199 11 amplified amplify VBN cord-275413-e2rhioty 199 12 from from IN cord-275413-e2rhioty 199 13 all all DT cord-275413-e2rhioty 199 14 tissues tissue NNS cord-275413-e2rhioty 199 15 , , , cord-275413-e2rhioty 199 16 indicating indicate VBG cord-275413-e2rhioty 199 17 that that IN cord-275413-e2rhioty 199 18 the the DT cord-275413-e2rhioty 199 19 RNA RNA NNP cord-275413-e2rhioty 199 20 was be VBD cord-275413-e2rhioty 199 21 intact intact JJ cord-275413-e2rhioty 199 22 . . . cord-275413-e2rhioty 200 1 IL-10 IL-10 NNP cord-275413-e2rhioty 200 2 was be VBD cord-275413-e2rhioty 200 3 amplified amplify VBN cord-275413-e2rhioty 200 4 from from IN cord-275413-e2rhioty 200 5 lung lung NN cord-275413-e2rhioty 200 6 and and CC cord-275413-e2rhioty 200 7 mandibular mandibular JJ cord-275413-e2rhioty 200 8 lymph lymph NN cord-275413-e2rhioty 200 9 nodes node NNS cord-275413-e2rhioty 200 10 from from IN cord-275413-e2rhioty 200 11 two two CD cord-275413-e2rhioty 200 12 of of IN cord-275413-e2rhioty 200 13 the the DT cord-275413-e2rhioty 200 14 four four CD cord-275413-e2rhioty 200 15 control control NN cord-275413-e2rhioty 200 16 fetuses fetus NNS cord-275413-e2rhioty 200 17 and and CC cord-275413-e2rhioty 200 18 from from IN cord-275413-e2rhioty 200 19 all all DT cord-275413-e2rhioty 200 20 infected infect VBN cord-275413-e2rhioty 200 21 fetuses fetus NNS cord-275413-e2rhioty 200 22 . . . cord-275413-e2rhioty 201 1 The the DT cord-275413-e2rhioty 201 2 presence presence NN cord-275413-e2rhioty 201 3 of of IN cord-275413-e2rhioty 201 4 IL-10 IL-10 NNP cord-275413-e2rhioty 201 5 was be VBD cord-275413-e2rhioty 201 6 not not RB cord-275413-e2rhioty 201 7 unexpected unexpected JJ cord-275413-e2rhioty 201 8 , , , cord-275413-e2rhioty 201 9 since since IN cord-275413-e2rhioty 201 10 increased increase VBN cord-275413-e2rhioty 201 11 IL-10 IL-10 NNP cord-275413-e2rhioty 201 12 production production NN cord-275413-e2rhioty 201 13 is be VBZ cord-275413-e2rhioty 201 14 associated associate VBN cord-275413-e2rhioty 201 15 with with IN cord-275413-e2rhioty 201 16 fetal fetal JJ cord-275413-e2rhioty 201 17 T t NN cord-275413-e2rhioty 201 18 cell cell NN cord-275413-e2rhioty 201 19 responses response NNS cord-275413-e2rhioty 201 20 ( ( -LRB- cord-275413-e2rhioty 201 21 Lin Lin NNP cord-275413-e2rhioty 201 22 et et NNP cord-275413-e2rhioty 201 23 al al NNP cord-275413-e2rhioty 201 24 . . NNP cord-275413-e2rhioty 201 25 , , , cord-275413-e2rhioty 201 26 1993 1993 CD cord-275413-e2rhioty 201 27 ; ; : cord-275413-e2rhioty 202 1 Rainsford Rainsford NNP cord-275413-e2rhioty 202 2 and and CC cord-275413-e2rhioty 202 3 Reen Reen NNP cord-275413-e2rhioty 202 4 , , , cord-275413-e2rhioty 202 5 2002 2002 CD cord-275413-e2rhioty 202 6 ) ) -RRB- cord-275413-e2rhioty 202 7 . . . cord-275413-e2rhioty 203 1 As as IN cord-275413-e2rhioty 203 2 shown show VBN cord-275413-e2rhioty 203 3 in in IN cord-275413-e2rhioty 203 4 Fig Fig NNP cord-275413-e2rhioty 203 5 . . . cord-275413-e2rhioty 204 1 4A 4a CD cord-275413-e2rhioty 204 2 , , , cord-275413-e2rhioty 205 1 IFN- IFN- NNP cord-275413-e2rhioty 205 2 ␥ ␥ CD cord-275413-e2rhioty 205 3 and and CC cord-275413-e2rhioty 205 4 TNF- TNF- NNP cord-275413-e2rhioty 205 5 ␣ ␣ NN cord-275413-e2rhioty 205 6 PCR PCR NNP cord-275413-e2rhioty 205 7 products product NNS cord-275413-e2rhioty 205 8 were be VBD cord-275413-e2rhioty 205 9 not not RB cord-275413-e2rhioty 205 10 detected detect VBN cord-275413-e2rhioty 205 11 in in IN cord-275413-e2rhioty 205 12 lung lung NN cord-275413-e2rhioty 205 13 , , , cord-275413-e2rhioty 205 14 lymph lymph NN cord-275413-e2rhioty 205 15 node node NN cord-275413-e2rhioty 205 16 or or CC cord-275413-e2rhioty 205 17 placenta placenta NN cord-275413-e2rhioty 205 18 from from IN cord-275413-e2rhioty 205 19 the the DT cord-275413-e2rhioty 205 20 non non JJ cord-275413-e2rhioty 205 21 - - JJ cord-275413-e2rhioty 205 22 infected infected JJ cord-275413-e2rhioty 205 23 fetuses fetus NNS cord-275413-e2rhioty 205 24 . . . cord-275413-e2rhioty 206 1 However however RB cord-275413-e2rhioty 206 2 , , , cord-275413-e2rhioty 206 3 IFN- IFN- NNP cord-275413-e2rhioty 206 4 ␥ ␥ CD cord-275413-e2rhioty 206 5 PCR PCR NNP cord-275413-e2rhioty 206 6 products product NNS cord-275413-e2rhioty 206 7 were be VBD cord-275413-e2rhioty 206 8 obtained obtain VBN cord-275413-e2rhioty 206 9 for for IN cord-275413-e2rhioty 206 10 lung lung NN cord-275413-e2rhioty 206 11 and and CC cord-275413-e2rhioty 206 12 lymph lymph NN cord-275413-e2rhioty 206 13 nodes node NNS cord-275413-e2rhioty 206 14 from from IN cord-275413-e2rhioty 206 15 infected infected JJ cord-275413-e2rhioty 206 16 fetuses fetus NNS cord-275413-e2rhioty 206 17 . . . cord-275413-e2rhioty 207 1 One one CD cord-275413-e2rhioty 207 2 infected infected JJ cord-275413-e2rhioty 207 3 fetus fetus NN cord-275413-e2rhioty 207 4 , , , cord-275413-e2rhioty 207 5 4 4 CD cord-275413-e2rhioty 207 6 - - SYM cord-275413-e2rhioty 207 7 3 3 CD cord-275413-e2rhioty 207 8 , , , cord-275413-e2rhioty 207 9 yielded yield VBD cord-275413-e2rhioty 207 10 a a DT cord-275413-e2rhioty 207 11 faint faint JJ cord-275413-e2rhioty 207 12 IFN- IFN- NNP cord-275413-e2rhioty 207 13 ␥ ␥ CD cord-275413-e2rhioty 207 14 product product NN cord-275413-e2rhioty 207 15 for for IN cord-275413-e2rhioty 207 16 placenta placenta NN cord-275413-e2rhioty 207 17 . . . cord-275413-e2rhioty 208 1 For for IN cord-275413-e2rhioty 208 2 infected infected JJ cord-275413-e2rhioty 208 3 fetuses fetus NNS cord-275413-e2rhioty 208 4 , , , cord-275413-e2rhioty 208 5 TNF- TNF- NNP cord-275413-e2rhioty 208 6 ␣ ␣ NNP cord-275413-e2rhioty 208 7 mRNA mrna NN cord-275413-e2rhioty 208 8 was be VBD cord-275413-e2rhioty 208 9 amplified amplify VBN cord-275413-e2rhioty 208 10 from from IN cord-275413-e2rhioty 208 11 lung lung NN cord-275413-e2rhioty 208 12 , , , cord-275413-e2rhioty 208 13 but but CC cord-275413-e2rhioty 208 14 not not RB cord-275413-e2rhioty 208 15 lymph lymph NN cord-275413-e2rhioty 208 16 node node NN cord-275413-e2rhioty 208 17 or or CC cord-275413-e2rhioty 208 18 placenta placenta NN cord-275413-e2rhioty 208 19 ( ( -LRB- cord-275413-e2rhioty 208 20 Fig fig NN cord-275413-e2rhioty 208 21 . . . cord-275413-e2rhioty 208 22 4B 4b CD cord-275413-e2rhioty 208 23 ) ) -RRB- cord-275413-e2rhioty 208 24 . . . cord-275413-e2rhioty 209 1 To to TO cord-275413-e2rhioty 209 2 determine determine VB cord-275413-e2rhioty 209 3 if if IN cord-275413-e2rhioty 209 4 cytokine cytokine NN cord-275413-e2rhioty 209 5 gene gene NN cord-275413-e2rhioty 209 6 expression expression NN cord-275413-e2rhioty 209 7 was be VBD cord-275413-e2rhioty 209 8 the the DT cord-275413-e2rhioty 209 9 direct direct JJ cord-275413-e2rhioty 209 10 result result NN cord-275413-e2rhioty 209 11 of of IN cord-275413-e2rhioty 209 12 PRRSV PRRSV NNP cord-275413-e2rhioty 209 13 infection infection NN cord-275413-e2rhioty 209 14 , , , cord-275413-e2rhioty 209 15 RT RT NNP cord-275413-e2rhioty 209 16 - - HYPH cord-275413-e2rhioty 209 17 PCR PCR NNP cord-275413-e2rhioty 209 18 for for IN cord-275413-e2rhioty 210 1 IFN- IFN- NNP cord-275413-e2rhioty 210 2 ␥ ␥ CD cord-275413-e2rhioty 210 3 and and CC cord-275413-e2rhioty 210 4 TNF- TNF- NNP cord-275413-e2rhioty 210 5 ␣ ␣ NNP cord-275413-e2rhioty 210 6 was be VBD cord-275413-e2rhioty 210 7 performed perform VBN cord-275413-e2rhioty 210 8 on on IN cord-275413-e2rhioty 210 9 the the DT cord-275413-e2rhioty 210 10 same same JJ cord-275413-e2rhioty 210 11 tissues tissue NNS cord-275413-e2rhioty 210 12 from from IN cord-275413-e2rhioty 210 13 fetuses fetus NNS cord-275413-e2rhioty 210 14 of of IN cord-275413-e2rhioty 210 15 infected infected JJ cord-275413-e2rhioty 210 16 dam dam NN cord-275413-e2rhioty 210 17 no no NN cord-275413-e2rhioty 210 18 . . NN cord-275413-e2rhioty 210 19 2 2 CD cord-275413-e2rhioty 210 20 , , , cord-275413-e2rhioty 210 21 which which WDT cord-275413-e2rhioty 210 22 produced produce VBD cord-275413-e2rhioty 210 23 only only RB cord-275413-e2rhioty 210 24 PRRSV PRRSV NNP cord-275413-e2rhioty 210 25 VI VI NNP cord-275413-e2rhioty 210 26 - - HYPH cord-275413-e2rhioty 210 27 negative negative JJ cord-275413-e2rhioty 210 28 fetuses fetus NNS cord-275413-e2rhioty 210 29 ( ( -LRB- cord-275413-e2rhioty 210 30 see see VB cord-275413-e2rhioty 210 31 Fig Fig NNP cord-275413-e2rhioty 210 32 . . NNP cord-275413-e2rhioty 210 33 1 1 CD cord-275413-e2rhioty 210 34 ) ) -RRB- cord-275413-e2rhioty 210 35 . . . cord-275413-e2rhioty 211 1 The the DT cord-275413-e2rhioty 211 2 analysis analysis NN cord-275413-e2rhioty 211 3 of of IN cord-275413-e2rhioty 211 4 mRNA mRNA NNP cord-275413-e2rhioty 211 5 expression expression NN cord-275413-e2rhioty 211 6 in in IN cord-275413-e2rhioty 211 7 lungs lung NNS cord-275413-e2rhioty 211 8 and and CC cord-275413-e2rhioty 211 9 lymph lymph NN cord-275413-e2rhioty 211 10 nodes node NNS cord-275413-e2rhioty 211 11 from from IN cord-275413-e2rhioty 211 12 six six CD cord-275413-e2rhioty 211 13 fetuses fetus NNS cord-275413-e2rhioty 211 14 from from IN cord-275413-e2rhioty 211 15 dam dam NN cord-275413-e2rhioty 211 16 no no NN cord-275413-e2rhioty 211 17 . . . cord-275413-e2rhioty 212 1 Fig Fig NNP cord-275413-e2rhioty 212 2 . . NNP cord-275413-e2rhioty 212 3 4 4 CD cord-275413-e2rhioty 212 4 . . . cord-275413-e2rhioty 213 1 Cytokine cytokine NN cord-275413-e2rhioty 213 2 gene gene NN cord-275413-e2rhioty 213 3 expression expression NN cord-275413-e2rhioty 213 4 in in IN cord-275413-e2rhioty 213 5 fetal fetal JJ cord-275413-e2rhioty 213 6 tissues tissue NNS cord-275413-e2rhioty 213 7 . . . cord-275413-e2rhioty 214 1 RT RT NNP cord-275413-e2rhioty 214 2 - - HYPH cord-275413-e2rhioty 214 3 PCR PCR NNP cord-275413-e2rhioty 214 4 for for IN cord-275413-e2rhioty 214 5 cytokine cytokine NN cord-275413-e2rhioty 214 6 mRNAs mrnas CD cord-275413-e2rhioty 214 7 was be VBD cord-275413-e2rhioty 214 8 performed perform VBN cord-275413-e2rhioty 214 9 on on IN cord-275413-e2rhioty 214 10 lung lung NN cord-275413-e2rhioty 214 11 ( ( -LRB- cord-275413-e2rhioty 214 12 L L NNP cord-275413-e2rhioty 214 13 ) ) -RRB- cord-275413-e2rhioty 214 14 , , , cord-275413-e2rhioty 214 15 mandibular mandibular JJ cord-275413-e2rhioty 214 16 lymph lymph NN cord-275413-e2rhioty 214 17 node node NN cord-275413-e2rhioty 214 18 ( ( -LRB- cord-275413-e2rhioty 214 19 N n NN cord-275413-e2rhioty 214 20 ) ) -RRB- cord-275413-e2rhioty 214 21 and and CC cord-275413-e2rhioty 214 22 placenta placenta NN cord-275413-e2rhioty 214 23 ( ( -LRB- cord-275413-e2rhioty 214 24 P p NN cord-275413-e2rhioty 214 25 ) ) -RRB- cord-275413-e2rhioty 214 26 for for IN cord-275413-e2rhioty 214 27 control control NN cord-275413-e2rhioty 214 28 ( ( -LRB- cord-275413-e2rhioty 214 29 panel panel NN cord-275413-e2rhioty 214 30 A A NNP cord-275413-e2rhioty 214 31 ) ) -RRB- cord-275413-e2rhioty 214 32 and and CC cord-275413-e2rhioty 214 33 infected infect VBN cord-275413-e2rhioty 214 34 ( ( -LRB- cord-275413-e2rhioty 214 35 panel panel NN cord-275413-e2rhioty 214 36 B b NN cord-275413-e2rhioty 214 37 ) ) -RRB- cord-275413-e2rhioty 214 38 fetuses fetus NNS cord-275413-e2rhioty 214 39 as as IN cord-275413-e2rhioty 214 40 described describe VBN cord-275413-e2rhioty 214 41 in in IN cord-275413-e2rhioty 214 42 the the DT cord-275413-e2rhioty 214 43 text text NN cord-275413-e2rhioty 214 44 using use VBG cord-275413-e2rhioty 214 45 the the DT cord-275413-e2rhioty 214 46 primers primer NNS cord-275413-e2rhioty 214 47 in in IN cord-275413-e2rhioty 214 48 Table Table NNP cord-275413-e2rhioty 214 49 2 2 CD cord-275413-e2rhioty 214 50 . . . cord-275413-e2rhioty 215 1 Amplification amplification NN cord-275413-e2rhioty 215 2 of of IN cord-275413-e2rhioty 216 1 ␤ ␤ IN cord-275413-e2rhioty 216 2 2-microglobulin 2-microglobulin CD cord-275413-e2rhioty 216 3 mRNA mrna NN cord-275413-e2rhioty 216 4 was be VBD cord-275413-e2rhioty 216 5 used use VBN cord-275413-e2rhioty 216 6 as as IN cord-275413-e2rhioty 216 7 an an DT cord-275413-e2rhioty 216 8 internal internal JJ cord-275413-e2rhioty 216 9 control control NN cord-275413-e2rhioty 216 10 . . . cord-275413-e2rhioty 217 1 PCR PCR NNP cord-275413-e2rhioty 217 2 products product NNS cord-275413-e2rhioty 217 3 were be VBD cord-275413-e2rhioty 217 4 electrophoresed electrophorese VBN cord-275413-e2rhioty 217 5 on on IN cord-275413-e2rhioty 217 6 a a DT cord-275413-e2rhioty 217 7 1.2 1.2 CD cord-275413-e2rhioty 217 8 % % NN cord-275413-e2rhioty 217 9 agarose agarose NN cord-275413-e2rhioty 217 10 gel gel NN cord-275413-e2rhioty 217 11 and and CC cord-275413-e2rhioty 217 12 stained stain VBN cord-275413-e2rhioty 217 13 with with IN cord-275413-e2rhioty 217 14 ethidium ethidium NN cord-275413-e2rhioty 217 15 bromide bromide NN cord-275413-e2rhioty 217 16 . . . cord-275413-e2rhioty 218 1 2 2 CD cord-275413-e2rhioty 218 2 showed show VBD cord-275413-e2rhioty 218 3 only only RB cord-275413-e2rhioty 218 4 the the DT cord-275413-e2rhioty 218 5 amplification amplification NN cord-275413-e2rhioty 218 6 of of IN cord-275413-e2rhioty 218 7 the the DT cord-275413-e2rhioty 218 8 ␤ ␤ NNP cord-275413-e2rhioty 218 9 2 2 CD cord-275413-e2rhioty 218 10 m m NN cord-275413-e2rhioty 218 11 , , , cord-275413-e2rhioty 218 12 but but CC cord-275413-e2rhioty 219 1 not not RB cord-275413-e2rhioty 219 2 TNF- tnf- RB cord-275413-e2rhioty 219 3 ␣ ␣ . cord-275413-e2rhioty 220 1 or or CC cord-275413-e2rhioty 220 2 IFN- IFN- NNP cord-275413-e2rhioty 220 3 ␥ ␥ XX cord-275413-e2rhioty 220 4 mRNAs mrnas XX cord-275413-e2rhioty 221 1 ( ( -LRB- cord-275413-e2rhioty 221 2 data datum NNS cord-275413-e2rhioty 221 3 not not RB cord-275413-e2rhioty 221 4 shown show VBN cord-275413-e2rhioty 221 5 ) ) -RRB- cord-275413-e2rhioty 221 6 . . . cord-275413-e2rhioty 222 1 Similar similar JJ cord-275413-e2rhioty 222 2 results result NNS cord-275413-e2rhioty 222 3 were be VBD cord-275413-e2rhioty 222 4 obtained obtain VBN cord-275413-e2rhioty 222 5 from from IN cord-275413-e2rhioty 222 6 virusnegative virusnegative JJ cord-275413-e2rhioty 222 7 fetuses fetus NNS cord-275413-e2rhioty 222 8 located locate VBN cord-275413-e2rhioty 222 9 immediately immediately RB cord-275413-e2rhioty 222 10 adjacent adjacent JJ cord-275413-e2rhioty 222 11 to to IN cord-275413-e2rhioty 222 12 infected infected JJ cord-275413-e2rhioty 222 13 fetuses fetus NNS cord-275413-e2rhioty 222 14 ( ( -LRB- cord-275413-e2rhioty 222 15 data datum NNS cord-275413-e2rhioty 222 16 not not RB cord-275413-e2rhioty 222 17 shown show VBN cord-275413-e2rhioty 222 18 ) ) -RRB- cord-275413-e2rhioty 222 19 . . . cord-275413-e2rhioty 223 1 In in IN cord-275413-e2rhioty 223 2 order order NN cord-275413-e2rhioty 223 3 to to TO cord-275413-e2rhioty 223 4 confirm confirm VB cord-275413-e2rhioty 223 5 that that IN cord-275413-e2rhioty 223 6 TNF- TNF- NNP cord-275413-e2rhioty 223 7 ␣ ␣ NN cord-275413-e2rhioty 223 8 and and CC cord-275413-e2rhioty 223 9 IFN- IFN- NNP cord-275413-e2rhioty 223 10 ␥ ␥ NNPS cord-275413-e2rhioty 223 11 were be VBD cord-275413-e2rhioty 223 12 produced produce VBN cord-275413-e2rhioty 223 13 during during IN cord-275413-e2rhioty 223 14 infection infection NN cord-275413-e2rhioty 223 15 , , , cord-275413-e2rhioty 223 16 cytokine cytokine NN cord-275413-e2rhioty 223 17 protein protein NN cord-275413-e2rhioty 223 18 levels level NNS cord-275413-e2rhioty 223 19 were be VBD cord-275413-e2rhioty 223 20 measured measure VBN cord-275413-e2rhioty 223 21 in in IN cord-275413-e2rhioty 223 22 fetal fetal JJ cord-275413-e2rhioty 223 23 sera sera NN cord-275413-e2rhioty 223 24 and and CC cord-275413-e2rhioty 223 25 amniotic amniotic NN cord-275413-e2rhioty 223 26 fluid fluid NN cord-275413-e2rhioty 223 27 from from IN cord-275413-e2rhioty 223 28 infected infected JJ cord-275413-e2rhioty 223 29 fetuses fetus NNS cord-275413-e2rhioty 223 30 and and CC cord-275413-e2rhioty 223 31 fetuses fetus NNS cord-275413-e2rhioty 223 32 from from IN cord-275413-e2rhioty 223 33 mock mock JJ cord-275413-e2rhioty 223 34 - - HYPH cord-275413-e2rhioty 223 35 infected infect VBN cord-275413-e2rhioty 223 36 dams dam NNS cord-275413-e2rhioty 223 37 . . . cord-275413-e2rhioty 224 1 As as IN cord-275413-e2rhioty 224 2 shown show VBN cord-275413-e2rhioty 224 3 in in IN cord-275413-e2rhioty 224 4 Fig Fig NNP cord-275413-e2rhioty 224 5 . . . cord-275413-e2rhioty 225 1 5 5 LS cord-275413-e2rhioty 225 2 , , , cord-275413-e2rhioty 225 3 the the DT cord-275413-e2rhioty 225 4 concentration concentration NN cord-275413-e2rhioty 225 5 of of IN cord-275413-e2rhioty 225 6 TNF- TNF- NNP cord-275413-e2rhioty 225 7 ␣ ␣ NN cord-275413-e2rhioty 225 8 in in IN cord-275413-e2rhioty 225 9 serum serum NN cord-275413-e2rhioty 225 10 for for IN cord-275413-e2rhioty 225 11 PRRSV PRRSV NNP cord-275413-e2rhioty 225 12 - - HYPH cord-275413-e2rhioty 225 13 infected infect VBN cord-275413-e2rhioty 225 14 fetuses fetus NNS cord-275413-e2rhioty 225 15 ranged range VBD cord-275413-e2rhioty 225 16 between between IN cord-275413-e2rhioty 225 17 20 20 CD cord-275413-e2rhioty 225 18 and and CC cord-275413-e2rhioty 225 19 100 100 CD cord-275413-e2rhioty 225 20 pg pg NN cord-275413-e2rhioty 225 21 / / SYM cord-275413-e2rhioty 225 22 ml ml NNS cord-275413-e2rhioty 225 23 compared compare VBN cord-275413-e2rhioty 225 24 to to IN cord-275413-e2rhioty 225 25 less less JJR cord-275413-e2rhioty 225 26 than than IN cord-275413-e2rhioty 225 27 30 30 CD cord-275413-e2rhioty 225 28 pg pg NN cord-275413-e2rhioty 225 29 / / SYM cord-275413-e2rhioty 225 30 ml ml NN cord-275413-e2rhioty 225 31 for for IN cord-275413-e2rhioty 225 32 control control NN cord-275413-e2rhioty 225 33 fetuses fetus NNS cord-275413-e2rhioty 225 34 . . . cord-275413-e2rhioty 226 1 Even even RB cord-275413-e2rhioty 226 2 though though IN cord-275413-e2rhioty 226 3 the the DT cord-275413-e2rhioty 226 4 maximum maximum JJ cord-275413-e2rhioty 226 5 quantities quantity NNS cord-275413-e2rhioty 226 6 obtained obtain VBN cord-275413-e2rhioty 226 7 for for IN cord-275413-e2rhioty 226 8 infected infected JJ cord-275413-e2rhioty 226 9 fetuses fetus NNS cord-275413-e2rhioty 226 10 were be VBD cord-275413-e2rhioty 226 11 near near IN cord-275413-e2rhioty 226 12 the the DT cord-275413-e2rhioty 226 13 lower low JJR cord-275413-e2rhioty 226 14 limit limit NN cord-275413-e2rhioty 226 15 of of IN cord-275413-e2rhioty 226 16 detection detection NN cord-275413-e2rhioty 226 17 for for IN cord-275413-e2rhioty 226 18 the the DT cord-275413-e2rhioty 226 19 ELISA ELISA NNP cord-275413-e2rhioty 226 20 test test NN cord-275413-e2rhioty 226 21 , , , cord-275413-e2rhioty 226 22 it -PRON- PRP cord-275413-e2rhioty 226 23 was be VBD cord-275413-e2rhioty 226 24 clear clear JJ cord-275413-e2rhioty 226 25 that that IN cord-275413-e2rhioty 226 26 TNF- TNF- NNP cord-275413-e2rhioty 226 27 ␣ ␣ NNP cord-275413-e2rhioty 226 28 was be VBD cord-275413-e2rhioty 226 29 elevated elevate VBN cord-275413-e2rhioty 226 30 during during IN cord-275413-e2rhioty 226 31 infection infection NN cord-275413-e2rhioty 226 32 . . . cord-275413-e2rhioty 227 1 Detectable detectable JJ cord-275413-e2rhioty 227 2 concentrations concentration NNS cord-275413-e2rhioty 227 3 of of IN cord-275413-e2rhioty 227 4 TNF- TNF- NNP cord-275413-e2rhioty 227 5 ␣ ␣ NN cord-275413-e2rhioty 227 6 ( ( -LRB- cord-275413-e2rhioty 227 7 > > NFP cord-275413-e2rhioty 227 8 23 23 CD cord-275413-e2rhioty 227 9 pg pg NNP cord-275413-e2rhioty 227 10 / / SYM cord-275413-e2rhioty 227 11 ml ml NN cord-275413-e2rhioty 227 12 ) ) -RRB- cord-275413-e2rhioty 227 13 were be VBD cord-275413-e2rhioty 227 14 found find VBN cord-275413-e2rhioty 227 15 in in IN cord-275413-e2rhioty 227 16 amniotic amniotic NN cord-275413-e2rhioty 227 17 fluid fluid NN cord-275413-e2rhioty 227 18 from from IN cord-275413-e2rhioty 227 19 only only RB cord-275413-e2rhioty 227 20 two two CD cord-275413-e2rhioty 227 21 control control NN cord-275413-e2rhioty 227 22 and and CC cord-275413-e2rhioty 227 23 two two CD cord-275413-e2rhioty 227 24 infected infected JJ cord-275413-e2rhioty 227 25 fetuses fetus NNS cord-275413-e2rhioty 227 26 . . . cord-275413-e2rhioty 228 1 Compared compare VBN cord-275413-e2rhioty 228 2 to to IN cord-275413-e2rhioty 228 3 fetuses fetus NNS cord-275413-e2rhioty 228 4 from from IN cord-275413-e2rhioty 228 5 mockinfected mockinfected JJ cord-275413-e2rhioty 228 6 dams dam NNS cord-275413-e2rhioty 228 7 , , , cord-275413-e2rhioty 228 8 IFN- IFN- NNP cord-275413-e2rhioty 228 9 ␥ ␥ CD cord-275413-e2rhioty 228 10 concentrations concentration NNS cord-275413-e2rhioty 228 11 were be VBD cord-275413-e2rhioty 228 12 detected detect VBN cord-275413-e2rhioty 228 13 in in IN cord-275413-e2rhioty 228 14 sera sera NNP cord-275413-e2rhioty 228 15 , , , cord-275413-e2rhioty 228 16 with with IN cord-275413-e2rhioty 228 17 values value NNS cord-275413-e2rhioty 228 18 ranging range VBG cord-275413-e2rhioty 228 19 from from IN cord-275413-e2rhioty 228 20 a a DT cord-275413-e2rhioty 228 21 low low NN cord-275413-e2rhioty 228 22 of of IN cord-275413-e2rhioty 228 23 60 60 CD cord-275413-e2rhioty 228 24 to to TO cord-275413-e2rhioty 228 25 more more JJR cord-275413-e2rhioty 228 26 than than IN cord-275413-e2rhioty 228 27 500 500 CD cord-275413-e2rhioty 228 28 pg pg NN cord-275413-e2rhioty 228 29 / / SYM cord-275413-e2rhioty 228 30 ml ml NN cord-275413-e2rhioty 228 31 . . . cord-275413-e2rhioty 229 1 A a DT cord-275413-e2rhioty 229 2 maximum maximum JJ cord-275413-e2rhioty 229 3 level level NN cord-275413-e2rhioty 229 4 of of IN cord-275413-e2rhioty 229 5 60 60 CD cord-275413-e2rhioty 229 6 pg pg NN cord-275413-e2rhioty 229 7 / / SYM cord-275413-e2rhioty 229 8 ml ml NN cord-275413-e2rhioty 229 9 was be VBD cord-275413-e2rhioty 229 10 obtained obtain VBN cord-275413-e2rhioty 229 11 for for IN cord-275413-e2rhioty 229 12 a a DT cord-275413-e2rhioty 229 13 single single JJ cord-275413-e2rhioty 229 14 non non JJ cord-275413-e2rhioty 229 15 - - JJ cord-275413-e2rhioty 229 16 infected infected JJ cord-275413-e2rhioty 229 17 fetus fetus NN cord-275413-e2rhioty 229 18 . . . cord-275413-e2rhioty 230 1 IFN- IFN- NNP cord-275413-e2rhioty 230 2 ␥ ␥ NNP cord-275413-e2rhioty 230 3 was be VBD cord-275413-e2rhioty 230 4 not not RB cord-275413-e2rhioty 230 5 detected detect VBN cord-275413-e2rhioty 230 6 in in IN cord-275413-e2rhioty 230 7 amniotic amniotic NN cord-275413-e2rhioty 230 8 fluid fluid NN cord-275413-e2rhioty 230 9 from from IN cord-275413-e2rhioty 230 10 either either CC cord-275413-e2rhioty 230 11 the the DT cord-275413-e2rhioty 230 12 infected infected JJ cord-275413-e2rhioty 230 13 or or CC cord-275413-e2rhioty 230 14 control control NN cord-275413-e2rhioty 230 15 fetuses fetus NNS cord-275413-e2rhioty 230 16 . . . cord-275413-e2rhioty 231 1 The the DT cord-275413-e2rhioty 231 2 presence presence NN cord-275413-e2rhioty 231 3 of of IN cord-275413-e2rhioty 232 1 IFN- IFN- NNP cord-275413-e2rhioty 232 2 ␥ ␥ CD cord-275413-e2rhioty 232 3 and and CC cord-275413-e2rhioty 232 4 TNF- TNF- NNP cord-275413-e2rhioty 232 5 ␣ ␣ NN cord-275413-e2rhioty 232 6 in in IN cord-275413-e2rhioty 232 7 serum serum NN cord-275413-e2rhioty 232 8 , , , cord-275413-e2rhioty 232 9 but but CC cord-275413-e2rhioty 232 10 not not RB cord-275413-e2rhioty 232 11 amniotic amniotic NN cord-275413-e2rhioty 232 12 fluid fluid NN cord-275413-e2rhioty 232 13 , , , cord-275413-e2rhioty 232 14 further further RB cord-275413-e2rhioty 232 15 supports support VBZ cord-275413-e2rhioty 232 16 the the DT cord-275413-e2rhioty 232 17 notion notion NN cord-275413-e2rhioty 232 18 that that IN cord-275413-e2rhioty 232 19 the the DT cord-275413-e2rhioty 232 20 IFN- IFN- NNP cord-275413-e2rhioty 232 21 ␥ ␥ NNP cord-275413-e2rhioty 232 22 and and CC cord-275413-e2rhioty 232 23 TNF- TNF- NNP cord-275413-e2rhioty 232 24 ␣ ␣ JJ cord-275413-e2rhioty 232 25 responses response NNS cord-275413-e2rhioty 232 26 are be VBP cord-275413-e2rhioty 232 27 primarily primarily RB cord-275413-e2rhioty 232 28 restricted restrict VBN cord-275413-e2rhioty 232 29 to to IN cord-275413-e2rhioty 232 30 the the DT cord-275413-e2rhioty 232 31 PRRSV PRRSV NNP cord-275413-e2rhioty 232 32 - - HYPH cord-275413-e2rhioty 232 33 infected infect VBN cord-275413-e2rhioty 232 34 fetus fetus NN cord-275413-e2rhioty 232 35 and and CC cord-275413-e2rhioty 232 36 do do VBP cord-275413-e2rhioty 232 37 not not RB cord-275413-e2rhioty 232 38 extend extend VB cord-275413-e2rhioty 232 39 to to IN cord-275413-e2rhioty 232 40 the the DT cord-275413-e2rhioty 232 41 accessory accessory JJ cord-275413-e2rhioty 232 42 tissue tissue NN cord-275413-e2rhioty 232 43 compartments compartment NNS cord-275413-e2rhioty 232 44 . . . cord-275413-e2rhioty 233 1 This this DT cord-275413-e2rhioty 233 2 study study NN cord-275413-e2rhioty 233 3 characterizes characterize VBZ cord-275413-e2rhioty 233 4 the the DT cord-275413-e2rhioty 233 5 unique unique JJ cord-275413-e2rhioty 233 6 biology biology NN cord-275413-e2rhioty 233 7 associated associate VBN cord-275413-e2rhioty 233 8 with with IN cord-275413-e2rhioty 233 9 the the DT cord-275413-e2rhioty 233 10 interaction interaction NN cord-275413-e2rhioty 233 11 between between IN cord-275413-e2rhioty 233 12 PRRSV PRRSV NNP cord-275413-e2rhioty 233 13 and and CC cord-275413-e2rhioty 233 14 the the DT cord-275413-e2rhioty 233 15 late late JJ cord-275413-e2rhioty 233 16 gestation gestation NN cord-275413-e2rhioty 233 17 fetus fetus NN cord-275413-e2rhioty 233 18 . . . cord-275413-e2rhioty 234 1 Consistent consistent JJ cord-275413-e2rhioty 234 2 with with IN cord-275413-e2rhioty 234 3 the the DT cord-275413-e2rhioty 234 4 body body NN cord-275413-e2rhioty 234 5 of of IN cord-275413-e2rhioty 234 6 published publish VBN cord-275413-e2rhioty 234 7 literature literature NN cord-275413-e2rhioty 234 8 , , , cord-275413-e2rhioty 234 9 the the DT cord-275413-e2rhioty 234 10 fetuses fetus NNS cord-275413-e2rhioty 234 11 from from IN cord-275413-e2rhioty 234 12 the the DT cord-275413-e2rhioty 234 13 infected infected JJ cord-275413-e2rhioty 234 14 dams dam NNS cord-275413-e2rhioty 234 15 obtained obtain VBN cord-275413-e2rhioty 234 16 in in IN cord-275413-e2rhioty 234 17 this this DT cord-275413-e2rhioty 234 18 study study NN cord-275413-e2rhioty 234 19 exhibited exhibit VBD cord-275413-e2rhioty 234 20 several several JJ cord-275413-e2rhioty 234 21 anatomic anatomic JJ cord-275413-e2rhioty 234 22 pathological pathological JJ cord-275413-e2rhioty 234 23 features feature NNS cord-275413-e2rhioty 234 24 typical typical JJ cord-275413-e2rhioty 234 25 of of IN cord-275413-e2rhioty 234 26 PRRSV PRRSV NNP cord-275413-e2rhioty 234 27 infection infection NN cord-275413-e2rhioty 234 28 of of IN cord-275413-e2rhioty 234 29 the the DT cord-275413-e2rhioty 234 30 fetus fetus NN cord-275413-e2rhioty 234 31 , , , cord-275413-e2rhioty 234 32 including include VBG cord-275413-e2rhioty 234 33 lesions lesion NNS cord-275413-e2rhioty 234 34 associated associate VBN cord-275413-e2rhioty 234 35 with with IN cord-275413-e2rhioty 234 36 the the DT cord-275413-e2rhioty 234 37 accessory accessory JJ cord-275413-e2rhioty 234 38 organs organ NNS cord-275413-e2rhioty 234 39 , , , cord-275413-e2rhioty 234 40 such such JJ cord-275413-e2rhioty 234 41 as as IN cord-275413-e2rhioty 234 42 umbilical umbilical JJ cord-275413-e2rhioty 234 43 cord cord NN cord-275413-e2rhioty 234 44 and and CC cord-275413-e2rhioty 234 45 amniotic amniotic NN cord-275413-e2rhioty 234 46 sac sac NN cord-275413-e2rhioty 234 47 ( ( -LRB- cord-275413-e2rhioty 234 48 Lager Lager NNP cord-275413-e2rhioty 234 49 and and CC cord-275413-e2rhioty 234 50 Halbur Halbur NNP cord-275413-e2rhioty 234 51 , , , cord-275413-e2rhioty 234 52 1996 1996 CD cord-275413-e2rhioty 234 53 ; ; : cord-275413-e2rhioty 234 54 Mengeling Mengeling NNP cord-275413-e2rhioty 234 55 et et FW cord-275413-e2rhioty 234 56 al al NNP cord-275413-e2rhioty 234 57 . . NNP cord-275413-e2rhioty 234 58 , , , cord-275413-e2rhioty 234 59 1996 1996 CD cord-275413-e2rhioty 234 60 ) ) -RRB- cord-275413-e2rhioty 234 61 . . . cord-275413-e2rhioty 235 1 One one CD cord-275413-e2rhioty 235 2 interesting interesting JJ cord-275413-e2rhioty 235 3 observation observation NN cord-275413-e2rhioty 235 4 from from IN cord-275413-e2rhioty 235 5 this this DT cord-275413-e2rhioty 235 6 study study NN cord-275413-e2rhioty 235 7 was be VBD cord-275413-e2rhioty 235 8 the the DT cord-275413-e2rhioty 235 9 apparent apparent JJ cord-275413-e2rhioty 235 10 absence absence NN cord-275413-e2rhioty 235 11 of of IN cord-275413-e2rhioty 235 12 a a DT cord-275413-e2rhioty 235 13 correlation correlation NN cord-275413-e2rhioty 235 14 between between IN cord-275413-e2rhioty 235 15 the the DT cord-275413-e2rhioty 235 16 presence presence NN cord-275413-e2rhioty 235 17 of of IN cord-275413-e2rhioty 235 18 gross gross JJ cord-275413-e2rhioty 235 19 abnormalities abnormality NNS cord-275413-e2rhioty 235 20 and and CC cord-275413-e2rhioty 235 21 productive productive JJ cord-275413-e2rhioty 235 22 fetal fetal JJ cord-275413-e2rhioty 235 23 infection infection NN cord-275413-e2rhioty 235 24 . . . cord-275413-e2rhioty 236 1 For for IN cord-275413-e2rhioty 236 2 example example NN cord-275413-e2rhioty 236 3 , , , cord-275413-e2rhioty 236 4 several several JJ cord-275413-e2rhioty 236 5 fetuses fetus NNS cord-275413-e2rhioty 236 6 from from IN cord-275413-e2rhioty 236 7 dam dam NN cord-275413-e2rhioty 236 8 no no NN cord-275413-e2rhioty 236 9 . . NN cord-275413-e2rhioty 236 10 2 2 CD cord-275413-e2rhioty 236 11 exhibited exhibit VBD cord-275413-e2rhioty 236 12 several several JJ cord-275413-e2rhioty 236 13 types type NNS cord-275413-e2rhioty 236 14 of of IN cord-275413-e2rhioty 236 15 gross gross JJ cord-275413-e2rhioty 236 16 pathology pathology NN cord-275413-e2rhioty 236 17 , , , cord-275413-e2rhioty 236 18 including include VBG cord-275413-e2rhioty 236 19 death death NN cord-275413-e2rhioty 236 20 , , , cord-275413-e2rhioty 236 21 growth growth NN cord-275413-e2rhioty 236 22 retardation retardation NN cord-275413-e2rhioty 236 23 , , , cord-275413-e2rhioty 236 24 or or CC cord-275413-e2rhioty 236 25 merconium merconium NN cord-275413-e2rhioty 236 26 / / SYM cord-275413-e2rhioty 236 27 blood blood NN cord-275413-e2rhioty 236 28 stained stain VBN cord-275413-e2rhioty 236 29 amniotic amniotic NN cord-275413-e2rhioty 236 30 fluid fluid NN cord-275413-e2rhioty 236 31 . . . cord-275413-e2rhioty 237 1 There there EX cord-275413-e2rhioty 237 2 were be VBD cord-275413-e2rhioty 237 3 also also RB cord-275413-e2rhioty 237 4 examples example NNS cord-275413-e2rhioty 237 5 of of IN cord-275413-e2rhioty 237 6 productively productively RB cord-275413-e2rhioty 237 7 infected infect VBN cord-275413-e2rhioty 237 8 fetuses fetus NNS cord-275413-e2rhioty 237 9 that that WDT cord-275413-e2rhioty 237 10 showed show VBD cord-275413-e2rhioty 237 11 no no DT cord-275413-e2rhioty 237 12 evidence evidence NN cord-275413-e2rhioty 237 13 of of IN cord-275413-e2rhioty 237 14 gross gross JJ cord-275413-e2rhioty 237 15 pathology pathology NN cord-275413-e2rhioty 237 16 ( ( -LRB- cord-275413-e2rhioty 237 17 see see VB cord-275413-e2rhioty 237 18 fetuses fetus NNS cord-275413-e2rhioty 237 19 1 1 CD cord-275413-e2rhioty 237 20 - - SYM cord-275413-e2rhioty 237 21 2 2 CD cord-275413-e2rhioty 237 22 , , , cord-275413-e2rhioty 237 23 3 3 CD cord-275413-e2rhioty 237 24 - - SYM cord-275413-e2rhioty 237 25 12 12 CD cord-275413-e2rhioty 237 26 , , , cord-275413-e2rhioty 237 27 4 4 CD cord-275413-e2rhioty 237 28 - - SYM cord-275413-e2rhioty 237 29 9 9 CD cord-275413-e2rhioty 237 30 , , , cord-275413-e2rhioty 237 31 4 4 CD cord-275413-e2rhioty 237 32 - - SYM cord-275413-e2rhioty 237 33 11 11 CD cord-275413-e2rhioty 237 34 , , , cord-275413-e2rhioty 237 35 4 4 CD cord-275413-e2rhioty 237 36 - - SYM cord-275413-e2rhioty 237 37 13 13 CD cord-275413-e2rhioty 237 38 in in IN cord-275413-e2rhioty 237 39 Fig Fig NNP cord-275413-e2rhioty 237 40 . . NNP cord-275413-e2rhioty 237 41 1 1 CD cord-275413-e2rhioty 237 42 ) ) -RRB- cord-275413-e2rhioty 237 43 . . . cord-275413-e2rhioty 238 1 The the DT cord-275413-e2rhioty 238 2 mechanism mechanism NN cord-275413-e2rhioty 238 3 for for IN cord-275413-e2rhioty 238 4 fetal fetal JJ cord-275413-e2rhioty 238 5 pathology pathology NN cord-275413-e2rhioty 238 6 remains remain VBZ cord-275413-e2rhioty 238 7 unclear unclear JJ cord-275413-e2rhioty 238 8 , , , cord-275413-e2rhioty 238 9 but but CC cord-275413-e2rhioty 238 10 the the DT cord-275413-e2rhioty 238 11 results result NNS cord-275413-e2rhioty 238 12 suggest suggest VBP cord-275413-e2rhioty 238 13 that that IN cord-275413-e2rhioty 238 14 the the DT cord-275413-e2rhioty 238 15 source source NN cord-275413-e2rhioty 238 16 pathology pathology NN cord-275413-e2rhioty 238 17 is be VBZ cord-275413-e2rhioty 238 18 likely likely JJ cord-275413-e2rhioty 238 19 result result NN cord-275413-e2rhioty 238 20 of of IN cord-275413-e2rhioty 238 21 the the DT cord-275413-e2rhioty 238 22 infection infection NN cord-275413-e2rhioty 238 23 of of IN cord-275413-e2rhioty 238 24 tissues tissue NNS cord-275413-e2rhioty 238 25 on on IN cord-275413-e2rhioty 238 26 the the DT cord-275413-e2rhioty 238 27 maternal maternal JJ cord-275413-e2rhioty 238 28 side side NN cord-275413-e2rhioty 238 29 and and CC cord-275413-e2rhioty 238 30 damage damage NN cord-275413-e2rhioty 238 31 to to IN cord-275413-e2rhioty 238 32 maternal maternal JJ cord-275413-e2rhioty 238 33 tissues tissue NNS cord-275413-e2rhioty 238 34 or or CC cord-275413-e2rhioty 238 35 production production NN cord-275413-e2rhioty 238 36 of of IN cord-275413-e2rhioty 238 37 maternal maternal JJ cord-275413-e2rhioty 238 38 factors factor NNS cord-275413-e2rhioty 238 39 that that WDT cord-275413-e2rhioty 238 40 affect affect VBP cord-275413-e2rhioty 238 41 the the DT cord-275413-e2rhioty 238 42 fetus fetus NN cord-275413-e2rhioty 238 43 . . . cord-275413-e2rhioty 239 1 In in IN cord-275413-e2rhioty 239 2 this this DT cord-275413-e2rhioty 239 3 study study NN cord-275413-e2rhioty 239 4 we -PRON- PRP cord-275413-e2rhioty 239 5 did do VBD cord-275413-e2rhioty 239 6 not not RB cord-275413-e2rhioty 239 7 observe observe VB cord-275413-e2rhioty 239 8 lesions lesion NNS cord-275413-e2rhioty 239 9 in in IN cord-275413-e2rhioty 239 10 the the DT cord-275413-e2rhioty 239 11 myometrium myometrium NN cord-275413-e2rhioty 239 12 or or CC cord-275413-e2rhioty 239 13 placenta placenta NN cord-275413-e2rhioty 239 14 ; ; : cord-275413-e2rhioty 239 15 however however RB cord-275413-e2rhioty 239 16 , , , cord-275413-e2rhioty 239 17 Stockhofe Stockhofe NNP cord-275413-e2rhioty 239 18 - - HYPH cord-275413-e2rhioty 239 19 Zurwieden Zurwieden NNP cord-275413-e2rhioty 239 20 et et NNP cord-275413-e2rhioty 239 21 al al NNP cord-275413-e2rhioty 239 22 . . . cord-275413-e2rhioty 240 1 ( ( -LRB- cord-275413-e2rhioty 240 2 1995 1995 CD cord-275413-e2rhioty 240 3 ) ) -RRB- cord-275413-e2rhioty 240 4 reported report VBD cord-275413-e2rhioty 240 5 PRRS PRRS NNP cord-275413-e2rhioty 240 6 virions virion NNS cord-275413-e2rhioty 240 7 budding bud VBG cord-275413-e2rhioty 240 8 from from IN cord-275413-e2rhioty 240 9 maternal maternal JJ cord-275413-e2rhioty 240 10 vascular vascular JJ cord-275413-e2rhioty 240 11 endothelial endothelial JJ cord-275413-e2rhioty 240 12 cells cell NNS cord-275413-e2rhioty 240 13 at at IN cord-275413-e2rhioty 240 14 the the DT cord-275413-e2rhioty 240 15 maternal maternal JJ cord-275413-e2rhioty 240 16 - - HYPH cord-275413-e2rhioty 240 17 fetal fetal JJ cord-275413-e2rhioty 240 18 interface interface NN cord-275413-e2rhioty 240 19 . . . cord-275413-e2rhioty 241 1 Lager Lager NNP cord-275413-e2rhioty 241 2 and and CC cord-275413-e2rhioty 241 3 Halbur Halbur NNP cord-275413-e2rhioty 241 4 ( ( -LRB- cord-275413-e2rhioty 241 5 1996 1996 CD cord-275413-e2rhioty 241 6 ) ) -RRB- cord-275413-e2rhioty 241 7 reported report VBD cord-275413-e2rhioty 241 8 damage damage NN cord-275413-e2rhioty 241 9 to to IN cord-275413-e2rhioty 241 10 the the DT cord-275413-e2rhioty 241 11 myometrium myometrium NN cord-275413-e2rhioty 241 12 during during IN cord-275413-e2rhioty 241 13 PRRSV PRRSV NNP cord-275413-e2rhioty 241 14 infection infection NN cord-275413-e2rhioty 241 15 . . . cord-275413-e2rhioty 242 1 Virus virus NN cord-275413-e2rhioty 242 2 - - HYPH cord-275413-e2rhioty 242 3 associated associate VBN cord-275413-e2rhioty 242 4 lesions lesion NNS cord-275413-e2rhioty 242 5 in in IN cord-275413-e2rhioty 242 6 the the DT cord-275413-e2rhioty 242 7 myometrium myometrium NN cord-275413-e2rhioty 242 8 are be VBP cord-275413-e2rhioty 242 9 observed observe VBN cord-275413-e2rhioty 242 10 for for IN cord-275413-e2rhioty 242 11 horses horse NNS cord-275413-e2rhioty 242 12 infected infect VBN cord-275413-e2rhioty 242 13 with with IN cord-275413-e2rhioty 242 14 EAV EAV NNP cord-275413-e2rhioty 242 15 ( ( -LRB- cord-275413-e2rhioty 242 16 Coignoul Coignoul NNP cord-275413-e2rhioty 242 17 and and CC cord-275413-e2rhioty 242 18 Cheville Cheville NNP cord-275413-e2rhioty 242 19 , , , cord-275413-e2rhioty 242 20 1984 1984 CD cord-275413-e2rhioty 242 21 ) ) -RRB- cord-275413-e2rhioty 242 22 . . . cord-275413-e2rhioty 243 1 The the DT cord-275413-e2rhioty 243 2 appar appar NNP cord-275413-e2rhioty 243 3 - - HYPH cord-275413-e2rhioty 243 4 ent ent NNP cord-275413-e2rhioty 243 5 discrepancy discrepancy NN cord-275413-e2rhioty 243 6 between between IN cord-275413-e2rhioty 243 7 pathology pathology NN cord-275413-e2rhioty 243 8 and and CC cord-275413-e2rhioty 243 9 infection infection NN cord-275413-e2rhioty 243 10 helps help VBZ cord-275413-e2rhioty 243 11 to to TO cord-275413-e2rhioty 243 12 explain explain VB cord-275413-e2rhioty 243 13 the the DT cord-275413-e2rhioty 243 14 stealthy stealthy JJ cord-275413-e2rhioty 243 15 nature nature NN cord-275413-e2rhioty 243 16 of of IN cord-275413-e2rhioty 243 17 the the DT cord-275413-e2rhioty 243 18 PRRSV PRRSV NNP cord-275413-e2rhioty 243 19 . . . cord-275413-e2rhioty 244 1 A a DT cord-275413-e2rhioty 244 2 significant significant JJ cord-275413-e2rhioty 244 3 number number NN cord-275413-e2rhioty 244 4 of of IN cord-275413-e2rhioty 244 5 apparently apparently RB cord-275413-e2rhioty 244 6 healthy healthy JJ cord-275413-e2rhioty 244 7 , , , cord-275413-e2rhioty 244 8 but but CC cord-275413-e2rhioty 244 9 infected infected JJ cord-275413-e2rhioty 244 10 fetuses fetus NNS cord-275413-e2rhioty 244 11 , , , cord-275413-e2rhioty 244 12 are be VBP cord-275413-e2rhioty 244 13 likely likely RB cord-275413-e2rhioty 244 14 go go VB cord-275413-e2rhioty 244 15 on on RP cord-275413-e2rhioty 244 16 to to TO cord-275413-e2rhioty 244 17 become become VB cord-275413-e2rhioty 244 18 healthy healthy JJ cord-275413-e2rhioty 244 19 growing grow VBG cord-275413-e2rhioty 244 20 pigs pig NNS cord-275413-e2rhioty 244 21 with with IN cord-275413-e2rhioty 244 22 the the DT cord-275413-e2rhioty 244 23 capacity capacity NN cord-275413-e2rhioty 244 24 to to TO cord-275413-e2rhioty 244 25 shed shed VB cord-275413-e2rhioty 244 26 virus virus NN cord-275413-e2rhioty 244 27 . . . cord-275413-e2rhioty 245 1 RNA RNA NNP cord-275413-e2rhioty 245 2 viruses virus NNS cord-275413-e2rhioty 245 3 often often RB cord-275413-e2rhioty 245 4 exist exist VBP cord-275413-e2rhioty 245 5 as as IN cord-275413-e2rhioty 245 6 a a DT cord-275413-e2rhioty 245 7 population population NN cord-275413-e2rhioty 245 8 of of IN cord-275413-e2rhioty 245 9 closely closely RB cord-275413-e2rhioty 245 10 related related JJ cord-275413-e2rhioty 245 11 sequences sequence NNS cord-275413-e2rhioty 245 12 , , , cord-275413-e2rhioty 245 13 frequently frequently RB cord-275413-e2rhioty 245 14 referred refer VBD cord-275413-e2rhioty 245 15 to to IN cord-275413-e2rhioty 245 16 as as IN cord-275413-e2rhioty 245 17 a a DT cord-275413-e2rhioty 245 18 quasispecies quasispecie NNS cord-275413-e2rhioty 245 19 . . . cord-275413-e2rhioty 246 1 The the DT cord-275413-e2rhioty 246 2 appearance appearance NN cord-275413-e2rhioty 246 3 or or CC cord-275413-e2rhioty 246 4 disappearance disappearance NN cord-275413-e2rhioty 246 5 of of IN cord-275413-e2rhioty 246 6 individual individual JJ cord-275413-e2rhioty 246 7 gene gene NN cord-275413-e2rhioty 246 8 sequences sequence NNS cord-275413-e2rhioty 246 9 in in IN cord-275413-e2rhioty 246 10 the the DT cord-275413-e2rhioty 246 11 population population NN cord-275413-e2rhioty 246 12 over over IN cord-275413-e2rhioty 246 13 the the DT cord-275413-e2rhioty 246 14 course course NN cord-275413-e2rhioty 246 15 of of IN cord-275413-e2rhioty 246 16 infection infection NN cord-275413-e2rhioty 246 17 is be VBZ cord-275413-e2rhioty 246 18 used use VBN cord-275413-e2rhioty 246 19 as as IN cord-275413-e2rhioty 246 20 evidence evidence NN cord-275413-e2rhioty 246 21 for for IN cord-275413-e2rhioty 246 22 changes change NNS cord-275413-e2rhioty 246 23 in in IN cord-275413-e2rhioty 246 24 fitness fitness NN cord-275413-e2rhioty 246 25 that that WDT cord-275413-e2rhioty 246 26 result result VBP cord-275413-e2rhioty 246 27 from from IN cord-275413-e2rhioty 246 28 selection selection NN cord-275413-e2rhioty 246 29 . . . cord-275413-e2rhioty 247 1 PRRSV PRRSV NNP cord-275413-e2rhioty 247 2 variants variant NNS cord-275413-e2rhioty 247 3 with with IN cord-275413-e2rhioty 247 4 mutations mutation NNS cord-275413-e2rhioty 247 5 in in IN cord-275413-e2rhioty 247 6 ORF5 ORF5 NNP cord-275413-e2rhioty 247 7 typically typically RB cord-275413-e2rhioty 247 8 appear appear VBP cord-275413-e2rhioty 247 9 during during IN cord-275413-e2rhioty 247 10 the the DT cord-275413-e2rhioty 247 11 serial serial JJ cord-275413-e2rhioty 247 12 passage passage NN cord-275413-e2rhioty 247 13 of of IN cord-275413-e2rhioty 247 14 virus virus NN cord-275413-e2rhioty 247 15 in in IN cord-275413-e2rhioty 247 16 culture culture NN cord-275413-e2rhioty 247 17 and and CC cord-275413-e2rhioty 247 18 during during IN cord-275413-e2rhioty 247 19 infection infection NN cord-275413-e2rhioty 247 20 of of IN cord-275413-e2rhioty 247 21 pigs pig NNS cord-275413-e2rhioty 247 22 Allende Allende NNP cord-275413-e2rhioty 247 23 et et NNP cord-275413-e2rhioty 247 24 al al NNP cord-275413-e2rhioty 247 25 . . NNP cord-275413-e2rhioty 247 26 , , , cord-275413-e2rhioty 247 27 2000 2000 CD cord-275413-e2rhioty 247 28 ) ) -RRB- cord-275413-e2rhioty 247 29 . . . cord-275413-e2rhioty 248 1 The the DT cord-275413-e2rhioty 248 2 significance significance NN cord-275413-e2rhioty 248 3 of of IN cord-275413-e2rhioty 248 4 mutations mutation NNS cord-275413-e2rhioty 248 5 in in IN cord-275413-e2rhioty 248 6 ORF5 ORF5 NNP cord-275413-e2rhioty 248 7 as as IN cord-275413-e2rhioty 248 8 a a DT cord-275413-e2rhioty 248 9 source source NN cord-275413-e2rhioty 248 10 for for IN cord-275413-e2rhioty 248 11 increased increased JJ cord-275413-e2rhioty 248 12 fitness fitness NN cord-275413-e2rhioty 248 13 during during IN cord-275413-e2rhioty 248 14 infection infection NN cord-275413-e2rhioty 248 15 is be VBZ cord-275413-e2rhioty 248 16 not not RB cord-275413-e2rhioty 248 17 completely completely RB cord-275413-e2rhioty 248 18 understood understand VBN cord-275413-e2rhioty 248 19 ; ; : cord-275413-e2rhioty 248 20 but but CC cord-275413-e2rhioty 248 21 is be VBZ cord-275413-e2rhioty 248 22 useful useful JJ cord-275413-e2rhioty 248 23 for for IN cord-275413-e2rhioty 248 24 following follow VBG cord-275413-e2rhioty 248 25 the the DT cord-275413-e2rhioty 248 26 fate fate NN cord-275413-e2rhioty 248 27 of of IN cord-275413-e2rhioty 248 28 individual individual JJ cord-275413-e2rhioty 248 29 PRRSV PRRSV NNP cord-275413-e2rhioty 248 30 subpopulations subpopulation NNS cord-275413-e2rhioty 248 31 over over IN cord-275413-e2rhioty 248 32 the the DT cord-275413-e2rhioty 248 33 course course NN cord-275413-e2rhioty 248 34 of of IN cord-275413-e2rhioty 248 35 a a DT cord-275413-e2rhioty 248 36 long long JJ cord-275413-e2rhioty 248 37 - - HYPH cord-275413-e2rhioty 248 38 term term NN cord-275413-e2rhioty 248 39 infection infection NN cord-275413-e2rhioty 248 40 . . . cord-275413-e2rhioty 249 1 In in IN cord-275413-e2rhioty 249 2 this this DT cord-275413-e2rhioty 249 3 study study NN cord-275413-e2rhioty 249 4 , , , cord-275413-e2rhioty 249 5 one one CD cord-275413-e2rhioty 249 6 dam dam NN cord-275413-e2rhioty 249 7 , , , cord-275413-e2rhioty 249 8 no no NN cord-275413-e2rhioty 249 9 . . NN cord-275413-e2rhioty 249 10 4 4 CD cord-275413-e2rhioty 249 11 , , , cord-275413-e2rhioty 249 12 showed show VBD cord-275413-e2rhioty 249 13 evidence evidence NN cord-275413-e2rhioty 249 14 of of IN cord-275413-e2rhioty 249 15 a a DT cord-275413-e2rhioty 249 16 mixed mixed JJ cord-275413-e2rhioty 249 17 PRRSV prrsv NN cord-275413-e2rhioty 249 18 infection infection NN cord-275413-e2rhioty 249 19 as as IN cord-275413-e2rhioty 249 20 indicated indicate VBN cord-275413-e2rhioty 249 21 by by IN cord-275413-e2rhioty 249 22 the the DT cord-275413-e2rhioty 249 23 presence presence NN cord-275413-e2rhioty 249 24 of of IN cord-275413-e2rhioty 249 25 two two CD cord-275413-e2rhioty 249 26 different different JJ cord-275413-e2rhioty 249 27 ORF5 orf5 NN cord-275413-e2rhioty 249 28 sequences sequence NNS cord-275413-e2rhioty 249 29 , , , cord-275413-e2rhioty 249 30 which which WDT cord-275413-e2rhioty 249 31 were be VBD cord-275413-e2rhioty 249 32 distinguished distinguish VBN cord-275413-e2rhioty 249 33 from from IN cord-275413-e2rhioty 249 34 each each DT cord-275413-e2rhioty 249 35 other other JJ cord-275413-e2rhioty 249 36 by by IN cord-275413-e2rhioty 249 37 a a DT cord-275413-e2rhioty 249 38 nucleotide nucleotide JJ cord-275413-e2rhioty 249 39 substitution substitution NN cord-275413-e2rhioty 249 40 at at IN cord-275413-e2rhioty 249 41 position position NN cord-275413-e2rhioty 249 42 77 77 CD cord-275413-e2rhioty 249 43 in in IN cord-275413-e2rhioty 249 44 ORF5 ORF5 NNP cord-275413-e2rhioty 249 45 . . . cord-275413-e2rhioty 250 1 The the DT cord-275413-e2rhioty 250 2 C C NNP cord-275413-e2rhioty 250 3 to to IN cord-275413-e2rhioty 250 4 T T NNP cord-275413-e2rhioty 250 5 change change NN cord-275413-e2rhioty 250 6 was be VBD cord-275413-e2rhioty 250 7 observed observe VBN cord-275413-e2rhioty 250 8 in in IN cord-275413-e2rhioty 250 9 approximately approximately RB cord-275413-e2rhioty 250 10 60 60 CD cord-275413-e2rhioty 250 11 % % NN cord-275413-e2rhioty 250 12 of of IN cord-275413-e2rhioty 250 13 the the DT cord-275413-e2rhioty 250 14 cloned clone VBN cord-275413-e2rhioty 250 15 plasmid plasmid NN cord-275413-e2rhioty 250 16 products product NNS cord-275413-e2rhioty 250 17 obtained obtain VBN cord-275413-e2rhioty 250 18 from from IN cord-275413-e2rhioty 250 19 dam dam NN cord-275413-e2rhioty 250 20 no no NN cord-275413-e2rhioty 250 21 . . NN cord-275413-e2rhioty 250 22 4 4 LS cord-275413-e2rhioty 250 23 ( ( -LRB- cord-275413-e2rhioty 250 24 see see VB cord-275413-e2rhioty 250 25 Table Table NNP cord-275413-e2rhioty 250 26 2 2 CD cord-275413-e2rhioty 250 27 ) ) -RRB- cord-275413-e2rhioty 250 28 . . . cord-275413-e2rhioty 251 1 However however RB cord-275413-e2rhioty 251 2 , , , cord-275413-e2rhioty 251 3 the the DT cord-275413-e2rhioty 251 4 same same JJ cord-275413-e2rhioty 251 5 frequency frequency NN cord-275413-e2rhioty 251 6 was be VBD cord-275413-e2rhioty 251 7 not not RB cord-275413-e2rhioty 251 8 transferred transfer VBN cord-275413-e2rhioty 251 9 to to IN cord-275413-e2rhioty 251 10 the the DT cord-275413-e2rhioty 251 11 individual individual JJ cord-275413-e2rhioty 251 12 fetuses fetus NNS cord-275413-e2rhioty 251 13 , , , cord-275413-e2rhioty 251 14 which which WDT cord-275413-e2rhioty 251 15 included include VBD cord-275413-e2rhioty 251 16 two two CD cord-275413-e2rhioty 251 17 fetuses fetus NNS cord-275413-e2rhioty 251 18 that that WDT cord-275413-e2rhioty 251 19 possessed possess VBD cord-275413-e2rhioty 251 20 only only RB cord-275413-e2rhioty 251 21 viruses virus NNS cord-275413-e2rhioty 251 22 with with IN cord-275413-e2rhioty 251 23 the the DT cord-275413-e2rhioty 251 24 T-77 T-77 NNP cord-275413-e2rhioty 251 25 mutation mutation NN cord-275413-e2rhioty 251 26 and and CC cord-275413-e2rhioty 251 27 a a DT cord-275413-e2rhioty 251 28 single single JJ cord-275413-e2rhioty 251 29 fetus fetus NN cord-275413-e2rhioty 251 30 with with IN cord-275413-e2rhioty 251 31 a a DT cord-275413-e2rhioty 251 32 virus virus NN cord-275413-e2rhioty 251 33 population population NN cord-275413-e2rhioty 251 34 dominated dominate VBN cord-275413-e2rhioty 251 35 by by IN cord-275413-e2rhioty 251 36 C C NNP cord-275413-e2rhioty 251 37 at at IN cord-275413-e2rhioty 251 38 position position NN cord-275413-e2rhioty 251 39 77 77 CD cord-275413-e2rhioty 251 40 . . . cord-275413-e2rhioty 252 1 These these DT cord-275413-e2rhioty 252 2 results result NNS cord-275413-e2rhioty 252 3 suggest suggest VBP cord-275413-e2rhioty 252 4 that that IN cord-275413-e2rhioty 252 5 fetal fetal JJ cord-275413-e2rhioty 252 6 infection infection NN cord-275413-e2rhioty 252 7 can can MD cord-275413-e2rhioty 252 8 alter alter VB cord-275413-e2rhioty 252 9 the the DT cord-275413-e2rhioty 252 10 selection selection NN cord-275413-e2rhioty 252 11 of of IN cord-275413-e2rhioty 252 12 PRRSV PRRSV NNP cord-275413-e2rhioty 252 13 variants variant NNS cord-275413-e2rhioty 252 14 and and CC cord-275413-e2rhioty 252 15 may may MD cord-275413-e2rhioty 252 16 represent represent VB cord-275413-e2rhioty 252 17 a a DT cord-275413-e2rhioty 252 18 source source NN cord-275413-e2rhioty 252 19 of of IN cord-275413-e2rhioty 252 20 PRRSV PRRSV NNP cord-275413-e2rhioty 252 21 genetic genetic JJ cord-275413-e2rhioty 252 22 diversity diversity NN cord-275413-e2rhioty 252 23 . . . cord-275413-e2rhioty 253 1 To to TO cord-275413-e2rhioty 253 2 identify identify VB cord-275413-e2rhioty 253 3 the the DT cord-275413-e2rhioty 253 4 targets target NNS cord-275413-e2rhioty 253 5 of of IN cord-275413-e2rhioty 253 6 virus virus NN cord-275413-e2rhioty 253 7 replication replication NN cord-275413-e2rhioty 253 8 in in IN cord-275413-e2rhioty 253 9 the the DT cord-275413-e2rhioty 253 10 fetus fetus NN cord-275413-e2rhioty 253 11 , , , cord-275413-e2rhioty 253 12 a a DT cord-275413-e2rhioty 253 13 variety variety NN cord-275413-e2rhioty 253 14 of of IN cord-275413-e2rhioty 253 15 tissues tissue NNS cord-275413-e2rhioty 253 16 were be VBD cord-275413-e2rhioty 253 17 assessed assess VBN cord-275413-e2rhioty 253 18 for for IN cord-275413-e2rhioty 253 19 the the DT cord-275413-e2rhioty 253 20 presence presence NN cord-275413-e2rhioty 253 21 of of IN cord-275413-e2rhioty 253 22 virus virus NN cord-275413-e2rhioty 253 23 and and CC cord-275413-e2rhioty 253 24 virus virus NN cord-275413-e2rhioty 253 25 - - HYPH cord-275413-e2rhioty 253 26 infected infect VBN cord-275413-e2rhioty 253 27 cells cell NNS cord-275413-e2rhioty 253 28 . . . cord-275413-e2rhioty 254 1 VI VI NNP cord-275413-e2rhioty 254 2 , , , cord-275413-e2rhioty 254 3 as as IN cord-275413-e2rhioty 254 4 opposed oppose VBN cord-275413-e2rhioty 254 5 to to IN cord-275413-e2rhioty 254 6 more more RBR cord-275413-e2rhioty 254 7 sensitive sensitive JJ cord-275413-e2rhioty 254 8 approaches approach NNS cord-275413-e2rhioty 254 9 , , , cord-275413-e2rhioty 254 10 such such JJ cord-275413-e2rhioty 254 11 as as IN cord-275413-e2rhioty 254 12 PCR PCR NNP cord-275413-e2rhioty 254 13 , , , cord-275413-e2rhioty 254 14 was be VBD cord-275413-e2rhioty 254 15 selected select VBN cord-275413-e2rhioty 254 16 as as IN cord-275413-e2rhioty 254 17 the the DT cord-275413-e2rhioty 254 18 means mean NNS cord-275413-e2rhioty 254 19 for for IN cord-275413-e2rhioty 254 20 measuring measure VBG cord-275413-e2rhioty 254 21 virus virus NN cord-275413-e2rhioty 254 22 , , , cord-275413-e2rhioty 254 23 because because IN cord-275413-e2rhioty 254 24 the the DT cord-275413-e2rhioty 254 25 relative relative JJ cord-275413-e2rhioty 254 26 insensitivity insensitivity NN cord-275413-e2rhioty 254 27 of of IN cord-275413-e2rhioty 254 28 VI VI NNP cord-275413-e2rhioty 254 29 avoids avoid VBZ cord-275413-e2rhioty 254 30 the the DT cord-275413-e2rhioty 254 31 possibility possibility NN cord-275413-e2rhioty 254 32 of of IN cord-275413-e2rhioty 254 33 false false JJ cord-275413-e2rhioty 254 34 positive positive JJ cord-275413-e2rhioty 254 35 PCR PCR NNP cord-275413-e2rhioty 254 36 results result NNS cord-275413-e2rhioty 254 37 that that WDT cord-275413-e2rhioty 254 38 might may MD cord-275413-e2rhioty 254 39 result result VB cord-275413-e2rhioty 254 40 from from IN cord-275413-e2rhioty 254 41 small small JJ cord-275413-e2rhioty 254 42 amounts amount NNS cord-275413-e2rhioty 254 43 of of IN cord-275413-e2rhioty 254 44 contaminating contaminate VBG cord-275413-e2rhioty 254 45 maternal maternal JJ cord-275413-e2rhioty 254 46 material material NN cord-275413-e2rhioty 254 47 . . . cord-275413-e2rhioty 255 1 Within within IN cord-275413-e2rhioty 255 2 the the DT cord-275413-e2rhioty 255 3 group group NN cord-275413-e2rhioty 255 4 of of IN cord-275413-e2rhioty 255 5 selected select VBN cord-275413-e2rhioty 255 6 tissues tissue NNS cord-275413-e2rhioty 255 7 , , , cord-275413-e2rhioty 255 8 virus virus NN cord-275413-e2rhioty 255 9 could could MD cord-275413-e2rhioty 255 10 be be VB cord-275413-e2rhioty 255 11 isolated isolate VBN cord-275413-e2rhioty 255 12 from from IN cord-275413-e2rhioty 255 13 all all DT cord-275413-e2rhioty 255 14 tissues tissue NNS cord-275413-e2rhioty 255 15 . . . cord-275413-e2rhioty 256 1 However however RB cord-275413-e2rhioty 256 2 , , , cord-275413-e2rhioty 256 3 the the DT cord-275413-e2rhioty 256 4 most most RBS cord-275413-e2rhioty 256 5 consistent consistent JJ cord-275413-e2rhioty 256 6 source source NN cord-275413-e2rhioty 256 7 and and CC cord-275413-e2rhioty 256 8 largest large JJS cord-275413-e2rhioty 256 9 quantity quantity NN cord-275413-e2rhioty 256 10 of of IN cord-275413-e2rhioty 256 11 virus virus NN cord-275413-e2rhioty 256 12 were be VBD cord-275413-e2rhioty 256 13 obtained obtain VBN cord-275413-e2rhioty 256 14 from from IN cord-275413-e2rhioty 256 15 the the DT cord-275413-e2rhioty 256 16 thymus thymus NN cord-275413-e2rhioty 256 17 . . . cord-275413-e2rhioty 257 1 Collectively collectively RB cord-275413-e2rhioty 257 2 , , , cord-275413-e2rhioty 257 3 these these DT cord-275413-e2rhioty 257 4 data datum NNS cord-275413-e2rhioty 257 5 identify identify VBP cord-275413-e2rhioty 257 6 the the DT cord-275413-e2rhioty 257 7 thymus thymus NN cord-275413-e2rhioty 257 8 as as IN cord-275413-e2rhioty 257 9 a a DT cord-275413-e2rhioty 257 10 primary primary JJ cord-275413-e2rhioty 257 11 site site NN cord-275413-e2rhioty 257 12 of of IN cord-275413-e2rhioty 257 13 virus virus NN cord-275413-e2rhioty 257 14 replication replication NN cord-275413-e2rhioty 257 15 in in IN cord-275413-e2rhioty 257 16 the the DT cord-275413-e2rhioty 257 17 PRRSV PRRSV NNP cord-275413-e2rhioty 257 18 - - HYPH cord-275413-e2rhioty 257 19 infected infect VBN cord-275413-e2rhioty 257 20 fetus fetus NN cord-275413-e2rhioty 257 21 and and CC cord-275413-e2rhioty 257 22 confirm confirm VB cord-275413-e2rhioty 257 23 an an DT cord-275413-e2rhioty 257 24 earlier early JJR cord-275413-e2rhioty 257 25 observation observation NN cord-275413-e2rhioty 257 26 for for IN cord-275413-e2rhioty 257 27 PRRSV PRRSV NNP cord-275413-e2rhioty 257 28 by by IN cord-275413-e2rhioty 257 29 Benson Benson NNP cord-275413-e2rhioty 257 30 et et NNP cord-275413-e2rhioty 257 31 al al NNP cord-275413-e2rhioty 257 32 . . . cord-275413-e2rhioty 258 1 ( ( -LRB- cord-275413-e2rhioty 258 2 2002 2002 CD cord-275413-e2rhioty 258 3 ) ) -RRB- cord-275413-e2rhioty 258 4 . . . cord-275413-e2rhioty 259 1 The the DT cord-275413-e2rhioty 259 2 specific specific JJ cord-275413-e2rhioty 259 3 cell cell NN cord-275413-e2rhioty 259 4 population population NN cord-275413-e2rhioty 259 5 in in IN cord-275413-e2rhioty 259 6 the the DT cord-275413-e2rhioty 259 7 thymus thymus NN cord-275413-e2rhioty 259 8 targeted target VBN cord-275413-e2rhioty 259 9 PRRSV PRRSV NNP cord-275413-e2rhioty 259 10 replication replication NN cord-275413-e2rhioty 259 11 remains remain VBZ cord-275413-e2rhioty 259 12 unknown unknown JJ cord-275413-e2rhioty 259 13 . . . cord-275413-e2rhioty 260 1 The the DT cord-275413-e2rhioty 260 2 natural natural JJ cord-275413-e2rhioty 260 3 predisposition predisposition NN cord-275413-e2rhioty 260 4 of of IN cord-275413-e2rhioty 260 5 maternal maternal JJ cord-275413-e2rhioty 260 6 immunity immunity NN cord-275413-e2rhioty 260 7 towards towards IN cord-275413-e2rhioty 260 8 Th2like th2like JJ cord-275413-e2rhioty 260 9 responses response NNS cord-275413-e2rhioty 260 10 aids aid NNS cord-275413-e2rhioty 260 11 in in IN cord-275413-e2rhioty 260 12 protecting protect VBG cord-275413-e2rhioty 260 13 the the DT cord-275413-e2rhioty 260 14 fetus fetus NN cord-275413-e2rhioty 260 15 by by IN cord-275413-e2rhioty 260 16 blocking block VBG cord-275413-e2rhioty 260 17 cell cell NN cord-275413-e2rhioty 260 18 - - HYPH cord-275413-e2rhioty 260 19 mediated mediate VBN cord-275413-e2rhioty 260 20 Th1-related Th1-related NNP cord-275413-e2rhioty 260 21 allorejection allorejection NN cord-275413-e2rhioty 260 22 responses response NNS cord-275413-e2rhioty 260 23 ( ( -LRB- cord-275413-e2rhioty 260 24 Arck Arck NNP cord-275413-e2rhioty 260 25 et et FW cord-275413-e2rhioty 260 26 al al NNP cord-275413-e2rhioty 260 27 . . NNP cord-275413-e2rhioty 260 28 , , , cord-275413-e2rhioty 260 29 1999 1999 CD cord-275413-e2rhioty 260 30 ; ; : cord-275413-e2rhioty 260 31 Entrican Entrican NNP cord-275413-e2rhioty 260 32 , , , cord-275413-e2rhioty 260 33 2002 2002 CD cord-275413-e2rhioty 260 34 ; ; : cord-275413-e2rhioty 260 35 Raghupathy Raghupathy NNP cord-275413-e2rhioty 260 36 , , , cord-275413-e2rhioty 260 37 2001 2001 CD cord-275413-e2rhioty 260 38 ; ; : cord-275413-e2rhioty 260 39 recently recently RB cord-275413-e2rhioty 260 40 reviewed review VBN cord-275413-e2rhioty 260 41 in in IN cord-275413-e2rhioty 260 42 Challis Challis NNP cord-275413-e2rhioty 260 43 et et NNP cord-275413-e2rhioty 260 44 al al NNP cord-275413-e2rhioty 260 45 . . NNP cord-275413-e2rhioty 260 46 , , , cord-275413-e2rhioty 260 47 2009 2009 CD cord-275413-e2rhioty 260 48 ) ) -RRB- cord-275413-e2rhioty 260 49 . . . cord-275413-e2rhioty 261 1 The the DT cord-275413-e2rhioty 261 2 negative negative JJ cord-275413-e2rhioty 261 3 influence influence NN cord-275413-e2rhioty 261 4 of of IN cord-275413-e2rhioty 261 5 Th1 th1 NN cord-275413-e2rhioty 261 6 cytokines cytokine NNS cord-275413-e2rhioty 261 7 on on IN cord-275413-e2rhioty 261 8 fetal fetal JJ cord-275413-e2rhioty 261 9 development development NN cord-275413-e2rhioty 261 10 can can MD cord-275413-e2rhioty 261 11 be be VB cord-275413-e2rhioty 261 12 demonstrated demonstrate VBN cord-275413-e2rhioty 261 13 experimentally experimentally RB cord-275413-e2rhioty 261 14 in in IN cord-275413-e2rhioty 261 15 mice mouse NNS cord-275413-e2rhioty 261 16 , , , cord-275413-e2rhioty 261 17 which which WDT cord-275413-e2rhioty 261 18 show show VBP cord-275413-e2rhioty 261 19 that that IN cord-275413-e2rhioty 261 20 a a DT cord-275413-e2rhioty 261 21 single single JJ cord-275413-e2rhioty 261 22 injection injection NN cord-275413-e2rhioty 261 23 of of IN cord-275413-e2rhioty 261 24 IL-2 IL-2 NNP cord-275413-e2rhioty 261 25 or or CC cord-275413-e2rhioty 261 26 IFN- IFN- NNP cord-275413-e2rhioty 261 27 ␥ ␥ NNP cord-275413-e2rhioty 261 28 into into IN cord-275413-e2rhioty 261 29 certain certain JJ cord-275413-e2rhioty 261 30 strains strain NNS cord-275413-e2rhioty 261 31 induces induce VBZ cord-275413-e2rhioty 261 32 fetal fetal JJ cord-275413-e2rhioty 261 33 resorption resorption NN cord-275413-e2rhioty 261 34 ( ( -LRB- cord-275413-e2rhioty 261 35 Lala Lala NNP cord-275413-e2rhioty 261 36 , , , cord-275413-e2rhioty 261 37 1990 1990 CD cord-275413-e2rhioty 261 38 ; ; : cord-275413-e2rhioty 261 39 Chaouat Chaouat NNP cord-275413-e2rhioty 261 40 et et NNP cord-275413-e2rhioty 261 41 al al NNP cord-275413-e2rhioty 261 42 . . NNP cord-275413-e2rhioty 261 43 , , , cord-275413-e2rhioty 261 44 1990 1990 CD cord-275413-e2rhioty 261 45 ) ) -RRB- cord-275413-e2rhioty 261 46 . . . cord-275413-e2rhioty 262 1 TNF- TNF- NNP cord-275413-e2rhioty 263 1 ␣ ␣ NNP cord-275413-e2rhioty 263 2 , , , cord-275413-e2rhioty 263 3 when when WRB cord-275413-e2rhioty 263 4 infected infect VBN cord-275413-e2rhioty 263 5 into into IN cord-275413-e2rhioty 263 6 mice mouse NNS cord-275413-e2rhioty 263 7 is be VBZ cord-275413-e2rhioty 263 8 abortifacient abortifacient JJ cord-275413-e2rhioty 264 1 ( ( -LRB- cord-275413-e2rhioty 264 2 Clark Clark NNP cord-275413-e2rhioty 264 3 et et NNP cord-275413-e2rhioty 264 4 al al NNP cord-275413-e2rhioty 264 5 . . NNP cord-275413-e2rhioty 264 6 , , , cord-275413-e2rhioty 264 7 2004 2004 CD cord-275413-e2rhioty 264 8 ) ) -RRB- cord-275413-e2rhioty 264 9 . . . cord-275413-e2rhioty 265 1 Th1 th1 NN cord-275413-e2rhioty 265 2 cytokines cytokine NNS cord-275413-e2rhioty 265 3 can can MD cord-275413-e2rhioty 265 4 also also RB cord-275413-e2rhioty 265 5 have have VB cord-275413-e2rhioty 265 6 important important JJ cord-275413-e2rhioty 265 7 long long JJ cord-275413-e2rhioty 265 8 - - HYPH cord-275413-e2rhioty 265 9 term term NN cord-275413-e2rhioty 265 10 impacts impact NNS cord-275413-e2rhioty 265 11 . . . cord-275413-e2rhioty 266 1 For for IN cord-275413-e2rhioty 266 2 example example NN cord-275413-e2rhioty 266 3 , , , cord-275413-e2rhioty 266 4 newborn newborn JJ cord-275413-e2rhioty 266 5 mice mouse NNS cord-275413-e2rhioty 266 6 that that WDT cord-275413-e2rhioty 266 7 survive survive VBP cord-275413-e2rhioty 266 8 maternal maternal JJ cord-275413-e2rhioty 266 9 infection infection NN cord-275413-e2rhioty 266 10 with with IN cord-275413-e2rhioty 266 11 influenza influenza NN cord-275413-e2rhioty 266 12 virus virus NN cord-275413-e2rhioty 266 13 exhibit exhibit NNP cord-275413-e2rhioty 266 14 behav behav NNP cord-275413-e2rhioty 266 15 - - HYPH cord-275413-e2rhioty 266 16 ior ior NNP cord-275413-e2rhioty 266 17 similar similar JJ cord-275413-e2rhioty 266 18 to to IN cord-275413-e2rhioty 266 19 hyperanxiety hyperanxiety NN cord-275413-e2rhioty 266 20 and and CC cord-275413-e2rhioty 266 21 autism autism NN cord-275413-e2rhioty 266 22 . . . cord-275413-e2rhioty 267 1 The the DT cord-275413-e2rhioty 267 2 behavioral behavioral JJ cord-275413-e2rhioty 267 3 changes change NNS cord-275413-e2rhioty 267 4 are be VBP cord-275413-e2rhioty 267 5 a a DT cord-275413-e2rhioty 267 6 consequence consequence NN cord-275413-e2rhioty 267 7 of of IN cord-275413-e2rhioty 267 8 the the DT cord-275413-e2rhioty 267 9 altered altered JJ cord-275413-e2rhioty 267 10 distribution distribution NN cord-275413-e2rhioty 267 11 of of IN cord-275413-e2rhioty 267 12 dopamine dopamine NN cord-275413-e2rhioty 267 13 and and CC cord-275413-e2rhioty 267 14 glutamine glutamine NN cord-275413-e2rhioty 267 15 receptors receptor NNS cord-275413-e2rhioty 267 16 during during IN cord-275413-e2rhioty 267 17 fetal fetal JJ cord-275413-e2rhioty 267 18 brain brain NN cord-275413-e2rhioty 267 19 development development NN cord-275413-e2rhioty 267 20 . . . cord-275413-e2rhioty 268 1 The the DT cord-275413-e2rhioty 268 2 effect effect NN cord-275413-e2rhioty 268 3 of of IN cord-275413-e2rhioty 268 4 influenza influenza NN cord-275413-e2rhioty 268 5 virus virus NN cord-275413-e2rhioty 268 6 infection infection NN cord-275413-e2rhioty 268 7 on on IN cord-275413-e2rhioty 268 8 the the DT cord-275413-e2rhioty 268 9 fetal fetal JJ cord-275413-e2rhioty 268 10 brain brain NN cord-275413-e2rhioty 268 11 can can MD cord-275413-e2rhioty 268 12 be be VB cord-275413-e2rhioty 268 13 mimicked mimic VBN cord-275413-e2rhioty 268 14 by by IN cord-275413-e2rhioty 268 15 the the DT cord-275413-e2rhioty 268 16 administration administration NN cord-275413-e2rhioty 268 17 of of IN cord-275413-e2rhioty 268 18 poly poly NNP cord-275413-e2rhioty 268 19 I I NNP cord-275413-e2rhioty 268 20 : : : cord-275413-e2rhioty 268 21 C C NNP cord-275413-e2rhioty 268 22 , , , cord-275413-e2rhioty 268 23 an an DT cord-275413-e2rhioty 268 24 inducer inducer NN cord-275413-e2rhioty 268 25 of of IN cord-275413-e2rhioty 268 26 IFN IFN NNP cord-275413-e2rhioty 268 27 ( ( -LRB- cord-275413-e2rhioty 268 28 Shi Shi NNP cord-275413-e2rhioty 268 29 et et FW cord-275413-e2rhioty 268 30 al al NNP cord-275413-e2rhioty 268 31 . . NNP cord-275413-e2rhioty 268 32 , , , cord-275413-e2rhioty 268 33 2003 2003 CD cord-275413-e2rhioty 268 34 ) ) -RRB- cord-275413-e2rhioty 268 35 . . . cord-275413-e2rhioty 269 1 Furthermore furthermore RB cord-275413-e2rhioty 269 2 , , , cord-275413-e2rhioty 269 3 the the DT cord-275413-e2rhioty 269 4 addition addition NN cord-275413-e2rhioty 269 5 of of IN cord-275413-e2rhioty 269 6 IFN- IFN- NNP cord-275413-e2rhioty 269 7 ␥ ␥ NNP cord-275413-e2rhioty 269 8 to to IN cord-275413-e2rhioty 269 9 hippocampal hippocampal JJ cord-275413-e2rhioty 269 10 neuron neuron NN cord-275413-e2rhioty 269 11 cultures culture NNS cord-275413-e2rhioty 269 12 alters alter VBZ cord-275413-e2rhioty 269 13 the the DT cord-275413-e2rhioty 269 14 distribution distribution NN cord-275413-e2rhioty 269 15 of of IN cord-275413-e2rhioty 269 16 glutamate glutamate NN cord-275413-e2rhioty 269 17 receptors receptor NNS cord-275413-e2rhioty 269 18 , , , cord-275413-e2rhioty 269 19 sufficient sufficient JJ cord-275413-e2rhioty 269 20 to to TO cord-275413-e2rhioty 269 21 affect affect VB cord-275413-e2rhioty 269 22 synaptic synaptic JJ cord-275413-e2rhioty 269 23 activity activity NN cord-275413-e2rhioty 269 24 between between IN cord-275413-e2rhioty 269 25 neurons neuron NNS cord-275413-e2rhioty 269 26 ( ( -LRB- cord-275413-e2rhioty 269 27 Vikman Vikman NNP cord-275413-e2rhioty 269 28 et et NNP cord-275413-e2rhioty 269 29 al al NNP cord-275413-e2rhioty 269 30 . . NNP cord-275413-e2rhioty 269 31 , , , cord-275413-e2rhioty 269 32 2001 2001 CD cord-275413-e2rhioty 269 33 ) ) -RRB- cord-275413-e2rhioty 269 34 . . . cord-275413-e2rhioty 270 1 Virus virus NN cord-275413-e2rhioty 270 2 infections infection NNS cord-275413-e2rhioty 270 3 during during IN cord-275413-e2rhioty 270 4 pregnancy pregnancy NN cord-275413-e2rhioty 270 5 present present VBP cord-275413-e2rhioty 270 6 an an DT cord-275413-e2rhioty 270 7 interesting interesting JJ cord-275413-e2rhioty 270 8 paradox paradox NN cord-275413-e2rhioty 270 9 : : : cord-275413-e2rhioty 270 10 those those DT cord-275413-e2rhioty 270 11 cytokines cytokine NNS cord-275413-e2rhioty 270 12 that that WDT cord-275413-e2rhioty 270 13 protect protect VBP cord-275413-e2rhioty 270 14 the the DT cord-275413-e2rhioty 270 15 fetus fetus NN cord-275413-e2rhioty 270 16 from from IN cord-275413-e2rhioty 270 17 viral viral JJ cord-275413-e2rhioty 270 18 infection infection NN cord-275413-e2rhioty 270 19 , , , cord-275413-e2rhioty 270 20 tend tend VBP cord-275413-e2rhioty 270 21 to to TO cord-275413-e2rhioty 270 22 inhibit inhibit VB cord-275413-e2rhioty 270 23 fetal fetal JJ cord-275413-e2rhioty 270 24 development development NN cord-275413-e2rhioty 270 25 or or CC cord-275413-e2rhioty 270 26 potentiate potentiate VB cord-275413-e2rhioty 270 27 rejection rejection NN cord-275413-e2rhioty 270 28 of of IN cord-275413-e2rhioty 270 29 the the DT cord-275413-e2rhioty 270 30 fetus fetus NN cord-275413-e2rhioty 270 31 ; ; : cord-275413-e2rhioty 270 32 while while IN cord-275413-e2rhioty 270 33 those those DT cord-275413-e2rhioty 270 34 cytokines cytokine NNS cord-275413-e2rhioty 270 35 that that WDT cord-275413-e2rhioty 270 36 maintain maintain VBP cord-275413-e2rhioty 270 37 and and CC cord-275413-e2rhioty 270 38 promote promote VB cord-275413-e2rhioty 270 39 fetal fetal JJ cord-275413-e2rhioty 270 40 development development NN cord-275413-e2rhioty 270 41 are be VBP cord-275413-e2rhioty 270 42 associated associate VBN cord-275413-e2rhioty 270 43 with with IN cord-275413-e2rhioty 270 44 the the DT cord-275413-e2rhioty 270 45 inhibition inhibition NN cord-275413-e2rhioty 270 46 of of IN cord-275413-e2rhioty 270 47 antiviral antiviral JJ cord-275413-e2rhioty 270 48 immune immune JJ cord-275413-e2rhioty 270 49 responses response NNS cord-275413-e2rhioty 270 50 . . . cord-275413-e2rhioty 271 1 Because because IN cord-275413-e2rhioty 271 2 of of IN cord-275413-e2rhioty 271 3 the the DT cord-275413-e2rhioty 271 4 potential potential JJ cord-275413-e2rhioty 271 5 negative negative JJ cord-275413-e2rhioty 271 6 impact impact NN cord-275413-e2rhioty 271 7 of of IN cord-275413-e2rhioty 271 8 Th1 th1 NN cord-275413-e2rhioty 271 9 cytokines cytokine NNS cord-275413-e2rhioty 271 10 on on IN cord-275413-e2rhioty 271 11 fetal fetal JJ cord-275413-e2rhioty 271 12 outcome outcome NN cord-275413-e2rhioty 271 13 , , , cord-275413-e2rhioty 271 14 there there EX cord-275413-e2rhioty 271 15 is be VBZ cord-275413-e2rhioty 271 16 a a DT cord-275413-e2rhioty 271 17 natural natural JJ cord-275413-e2rhioty 271 18 predisposition predisposition NN cord-275413-e2rhioty 271 19 for for IN cord-275413-e2rhioty 271 20 the the DT cord-275413-e2rhioty 271 21 fetus fetus NN cord-275413-e2rhioty 271 22 to to TO cord-275413-e2rhioty 271 23 block block VB cord-275413-e2rhioty 271 24 the the DT cord-275413-e2rhioty 271 25 induction induction NN cord-275413-e2rhioty 271 26 of of IN cord-275413-e2rhioty 271 27 Th1-associated Th1-associated NNP cord-275413-e2rhioty 271 28 cytokines cytokine NNS cord-275413-e2rhioty 271 29 . . . cord-275413-e2rhioty 272 1 For for IN cord-275413-e2rhioty 272 2 example example NN cord-275413-e2rhioty 272 3 , , , cord-275413-e2rhioty 272 4 the the DT cord-275413-e2rhioty 272 5 IFN- IFN- NNP cord-275413-e2rhioty 272 6 ␥ ␥ CD cord-275413-e2rhioty 272 7 response response NN cord-275413-e2rhioty 272 8 in in IN cord-275413-e2rhioty 272 9 the the DT cord-275413-e2rhioty 272 10 developing develop VBG cord-275413-e2rhioty 272 11 fetus fetus NN cord-275413-e2rhioty 272 12 can can MD cord-275413-e2rhioty 272 13 be be VB cord-275413-e2rhioty 272 14 blocked block VBN cord-275413-e2rhioty 272 15 by by IN cord-275413-e2rhioty 272 16 a a DT cord-275413-e2rhioty 272 17 combination combination NN cord-275413-e2rhioty 272 18 of of IN cord-275413-e2rhioty 272 19 factors factor NNS cord-275413-e2rhioty 272 20 , , , cord-275413-e2rhioty 272 21 including include VBG cord-275413-e2rhioty 272 22 ( ( -LRB- cord-275413-e2rhioty 272 23 1 1 CD cord-275413-e2rhioty 272 24 ) ) -RRB- cord-275413-e2rhioty 272 25 defects defect NNS cord-275413-e2rhioty 272 26 in in IN cord-275413-e2rhioty 272 27 the the DT cord-275413-e2rhioty 272 28 capacity capacity NN cord-275413-e2rhioty 272 29 of of IN cord-275413-e2rhioty 272 30 fetal fetal JJ cord-275413-e2rhioty 272 31 dendritic dendritic JJ cord-275413-e2rhioty 272 32 cells cell NNS cord-275413-e2rhioty 272 33 to to TO cord-275413-e2rhioty 272 34 express express VB cord-275413-e2rhioty 272 35 MHC MHC NNP cord-275413-e2rhioty 272 36 class class NN cord-275413-e2rhioty 272 37 II II NNP cord-275413-e2rhioty 272 38 and and CC cord-275413-e2rhioty 272 39 synthesize synthesize VB cord-275413-e2rhioty 272 40 IL-12 IL-12 NNP cord-275413-e2rhioty 272 41 , , , cord-275413-e2rhioty 272 42 ( ( -LRB- cord-275413-e2rhioty 272 43 2 2 LS cord-275413-e2rhioty 272 44 ) ) -RRB- cord-275413-e2rhioty 272 45 hypermethylation hypermethylation NN cord-275413-e2rhioty 272 46 of of IN cord-275413-e2rhioty 272 47 the the DT cord-275413-e2rhioty 272 48 IFN- IFN- NNP cord-275413-e2rhioty 272 49 ␥ ␥ CD cord-275413-e2rhioty 272 50 gene gene NN cord-275413-e2rhioty 272 51 in in IN cord-275413-e2rhioty 272 52 fetal fetal JJ cord-275413-e2rhioty 272 53 T t NN cord-275413-e2rhioty 272 54 cells cell NNS cord-275413-e2rhioty 272 55 , , , cord-275413-e2rhioty 272 56 ( ( -LRB- cord-275413-e2rhioty 272 57 3 3 LS cord-275413-e2rhioty 272 58 ) ) -RRB- cord-275413-e2rhioty 272 59 the the DT cord-275413-e2rhioty 272 60 absence absence NN cord-275413-e2rhioty 272 61 of of IN cord-275413-e2rhioty 272 62 target target NN cord-275413-e2rhioty 272 63 macrophages macrophage NNS cord-275413-e2rhioty 272 64 , , , cord-275413-e2rhioty 272 65 and and CC cord-275413-e2rhioty 272 66 ( ( -LRB- cord-275413-e2rhioty 272 67 4 4 LS cord-275413-e2rhioty 272 68 ) ) -RRB- cord-275413-e2rhioty 272 69 the the DT cord-275413-e2rhioty 272 70 presence presence NN cord-275413-e2rhioty 272 71 of of IN cord-275413-e2rhioty 272 72 an an DT cord-275413-e2rhioty 272 73 immune immune JJ cord-275413-e2rhioty 272 74 environment environment NN cord-275413-e2rhioty 272 75 dominated dominate VBN cord-275413-e2rhioty 272 76 by by IN cord-275413-e2rhioty 272 77 Th2 Th2 NNP cord-275413-e2rhioty 272 78 / / SYM cord-275413-e2rhioty 272 79 regulatory regulatory JJ cord-275413-e2rhioty 272 80 cytokines cytokine NNS cord-275413-e2rhioty 272 81 ( ( -LRB- cord-275413-e2rhioty 272 82 Goriely Goriely NNP cord-275413-e2rhioty 272 83 et et NNP cord-275413-e2rhioty 272 84 al al NNP cord-275413-e2rhioty 272 85 . . NNP cord-275413-e2rhioty 272 86 , , , cord-275413-e2rhioty 272 87 2001 2001 CD cord-275413-e2rhioty 272 88 ; ; : cord-275413-e2rhioty 272 89 Langrish Langrish NNP cord-275413-e2rhioty 272 90 et et NNP cord-275413-e2rhioty 272 91 al al NNP cord-275413-e2rhioty 272 92 . . NNP cord-275413-e2rhioty 272 93 , , , cord-275413-e2rhioty 272 94 2002 2002 CD cord-275413-e2rhioty 272 95 ; ; : cord-275413-e2rhioty 272 96 Melvin Melvin NNP cord-275413-e2rhioty 272 97 et et NNP cord-275413-e2rhioty 272 98 al al NNP cord-275413-e2rhioty 272 99 . . NNP cord-275413-e2rhioty 272 100 , , , cord-275413-e2rhioty 272 101 1995 1995 CD cord-275413-e2rhioty 272 102 ; ; : cord-275413-e2rhioty 272 103 Marodi Marodi NNP cord-275413-e2rhioty 272 104 et et NNP cord-275413-e2rhioty 272 105 al al NNP cord-275413-e2rhioty 272 106 . . . cord-275413-e2rhioty 273 1 , , , cord-275413-e2rhioty 273 2 2001 2001 CD cord-275413-e2rhioty 273 3 ; ; : cord-275413-e2rhioty 273 4 Murphy Murphy NNP cord-275413-e2rhioty 273 5 et et NNP cord-275413-e2rhioty 273 6 al al NNP cord-275413-e2rhioty 273 7 . . . cord-275413-e2rhioty 274 1 , , , cord-275413-e2rhioty 274 2 2009 2009 CD cord-275413-e2rhioty 274 3 ; ; : cord-275413-e2rhioty 274 4 Prescott Prescott NNP cord-275413-e2rhioty 274 5 et et NNP cord-275413-e2rhioty 274 6 al al NNP cord-275413-e2rhioty 274 7 . . NNP cord-275413-e2rhioty 274 8 , , , cord-275413-e2rhioty 274 9 1998 1998 CD cord-275413-e2rhioty 274 10 ; ; : cord-275413-e2rhioty 274 11 White White NNP cord-275413-e2rhioty 274 12 et et NNP cord-275413-e2rhioty 274 13 al al NNP cord-275413-e2rhioty 274 14 . . NNP cord-275413-e2rhioty 274 15 , , , cord-275413-e2rhioty 274 16 2002 2002 CD cord-275413-e2rhioty 274 17 , , , cord-275413-e2rhioty 274 18 ) ) -RRB- cord-275413-e2rhioty 274 19 . . . cord-275413-e2rhioty 275 1 With with IN cord-275413-e2rhioty 275 2 this this DT cord-275413-e2rhioty 275 3 in in IN cord-275413-e2rhioty 275 4 mind mind NN cord-275413-e2rhioty 275 5 , , , cord-275413-e2rhioty 275 6 there there EX cord-275413-e2rhioty 275 7 are be VBP cord-275413-e2rhioty 275 8 examples example NNS cord-275413-e2rhioty 275 9 of of IN cord-275413-e2rhioty 275 10 viruses virus NNS cord-275413-e2rhioty 275 11 , , , cord-275413-e2rhioty 275 12 such such JJ cord-275413-e2rhioty 275 13 as as IN cord-275413-e2rhioty 275 14 Epstein Epstein NNP cord-275413-e2rhioty 275 15 - - HYPH cord-275413-e2rhioty 275 16 Barr Barr NNP cord-275413-e2rhioty 275 17 virus virus NN cord-275413-e2rhioty 275 18 ( ( -LRB- cord-275413-e2rhioty 275 19 EBV EBV NNP cord-275413-e2rhioty 275 20 ) ) -RRB- cord-275413-e2rhioty 275 21 , , , cord-275413-e2rhioty 275 22 which which WDT cord-275413-e2rhioty 275 23 are be VBP cord-275413-e2rhioty 275 24 capable capable JJ cord-275413-e2rhioty 275 25 of of IN cord-275413-e2rhioty 275 26 stimulating stimulate VBG cord-275413-e2rhioty 275 27 antigen antigen NN cord-275413-e2rhioty 275 28 - - HYPH cord-275413-e2rhioty 275 29 specific specific JJ cord-275413-e2rhioty 275 30 IFN- IFN- NNP cord-275413-e2rhioty 275 31 ␥ ␥ CD cord-275413-e2rhioty 275 32 responses response NNS cord-275413-e2rhioty 275 33 in in IN cord-275413-e2rhioty 275 34 human human JJ cord-275413-e2rhioty 275 35 umbilical umbilical JJ cord-275413-e2rhioty 275 36 cord cord NN cord-275413-e2rhioty 275 37 blood blood NN cord-275413-e2rhioty 275 38 lymphocytes lymphocyte NNS cord-275413-e2rhioty 275 39 ( ( -LRB- cord-275413-e2rhioty 275 40 Ito Ito NNP cord-275413-e2rhioty 275 41 et et FW cord-275413-e2rhioty 275 42 al al NNP cord-275413-e2rhioty 275 43 . . NNP cord-275413-e2rhioty 275 44 , , , cord-275413-e2rhioty 275 45 1998 1998 CD cord-275413-e2rhioty 275 46 ; ; : cord-275413-e2rhioty 275 47 Wilson Wilson NNP cord-275413-e2rhioty 275 48 and and CC cord-275413-e2rhioty 275 49 Morgan Morgan NNP cord-275413-e2rhioty 275 50 , , , cord-275413-e2rhioty 275 51 2002 2002 CD cord-275413-e2rhioty 275 52 ) ) -RRB- cord-275413-e2rhioty 275 53 . . . cord-275413-e2rhioty 276 1 These these DT cord-275413-e2rhioty 276 2 and and CC cord-275413-e2rhioty 276 3 other other JJ cord-275413-e2rhioty 276 4 data datum NNS cord-275413-e2rhioty 276 5 provide provide VBP cord-275413-e2rhioty 276 6 a a DT cord-275413-e2rhioty 276 7 description description NN cord-275413-e2rhioty 276 8 of of IN cord-275413-e2rhioty 276 9 the the DT cord-275413-e2rhioty 276 10 fetus fetus NN cord-275413-e2rhioty 276 11 as as IN cord-275413-e2rhioty 276 12 capable capable JJ cord-275413-e2rhioty 276 13 of of IN cord-275413-e2rhioty 276 14 initiating initiate VBG cord-275413-e2rhioty 276 15 a a DT cord-275413-e2rhioty 276 16 robust robust JJ cord-275413-e2rhioty 276 17 antiviral antiviral JJ cord-275413-e2rhioty 276 18 Th1 th1 CD cord-275413-e2rhioty 276 19 immune immune JJ cord-275413-e2rhioty 276 20 response response NN cord-275413-e2rhioty 276 21 ( ( -LRB- cord-275413-e2rhioty 276 22 Chaouat Chaouat NNP cord-275413-e2rhioty 276 23 et et NNP cord-275413-e2rhioty 276 24 al al NNP cord-275413-e2rhioty 276 25 . . NNP cord-275413-e2rhioty 276 26 , , , cord-275413-e2rhioty 276 27 2002 2002 CD cord-275413-e2rhioty 276 28 ; ; : cord-275413-e2rhioty 276 29 Chipeta Chipeta NNP cord-275413-e2rhioty 276 30 et et FW cord-275413-e2rhioty 276 31 al al NNP cord-275413-e2rhioty 276 32 . . NNP cord-275413-e2rhioty 276 33 , , , cord-275413-e2rhioty 276 34 2000 2000 CD cord-275413-e2rhioty 276 35 ; ; : cord-275413-e2rhioty 276 36 Murphy Murphy NNP cord-275413-e2rhioty 276 37 et et NNP cord-275413-e2rhioty 276 38 al al NNP cord-275413-e2rhioty 276 39 . . NNP cord-275413-e2rhioty 276 40 , , , cord-275413-e2rhioty 276 41 2009 2009 CD cord-275413-e2rhioty 276 42 ) ) -RRB- cord-275413-e2rhioty 276 43 . . . cord-275413-e2rhioty 277 1 In in IN cord-275413-e2rhioty 277 2 this this DT cord-275413-e2rhioty 277 3 study study NN cord-275413-e2rhioty 277 4 IFN- IFN- NNP cord-275413-e2rhioty 277 5 ␥ ␥ NNP cord-275413-e2rhioty 277 6 and and CC cord-275413-e2rhioty 277 7 TNF- TNF- NNP cord-275413-e2rhioty 277 8 ␣ ␣ NNP cord-275413-e2rhioty 277 9 mRNAs mrnas PRP cord-275413-e2rhioty 277 10 were be VBD cord-275413-e2rhioty 277 11 identified identify VBN cord-275413-e2rhioty 277 12 in in IN cord-275413-e2rhioty 277 13 tissues tissue NNS cord-275413-e2rhioty 277 14 from from IN cord-275413-e2rhioty 277 15 infected infected JJ cord-275413-e2rhioty 277 16 fetuses fetus NNS cord-275413-e2rhioty 277 17 . . . cord-275413-e2rhioty 278 1 RNA RNA NNP cord-275413-e2rhioty 278 2 message message NN cord-275413-e2rhioty 278 3 was be VBD cord-275413-e2rhioty 278 4 not not RB cord-275413-e2rhioty 278 5 identified identify VBN cord-275413-e2rhioty 278 6 in in IN cord-275413-e2rhioty 278 7 tissues tissue NNS cord-275413-e2rhioty 278 8 of of IN cord-275413-e2rhioty 278 9 fetuses fetus NNS cord-275413-e2rhioty 278 10 from from IN cord-275413-e2rhioty 278 11 mock mock JJ cord-275413-e2rhioty 278 12 - - HYPH cord-275413-e2rhioty 278 13 infected infect VBN cord-275413-e2rhioty 278 14 dams dam NNS cord-275413-e2rhioty 278 15 or or CC cord-275413-e2rhioty 278 16 non non JJ cord-275413-e2rhioty 278 17 - - JJ cord-275413-e2rhioty 278 18 infected infected JJ cord-275413-e2rhioty 278 19 fetuses fetus NNS cord-275413-e2rhioty 278 20 from from IN cord-275413-e2rhioty 278 21 infected infected JJ cord-275413-e2rhioty 278 22 dams dam NNS cord-275413-e2rhioty 278 23 . . . cord-275413-e2rhioty 279 1 This this DT cord-275413-e2rhioty 279 2 indicates indicate VBZ cord-275413-e2rhioty 279 3 that that IN cord-275413-e2rhioty 279 4 altered alter VBN cord-275413-e2rhioty 279 5 gene gene NN cord-275413-e2rhioty 279 6 expression expression NN cord-275413-e2rhioty 279 7 is be VBZ cord-275413-e2rhioty 279 8 the the DT cord-275413-e2rhioty 279 9 direct direct JJ cord-275413-e2rhioty 279 10 result result NN cord-275413-e2rhioty 279 11 of of IN cord-275413-e2rhioty 279 12 fetal fetal JJ cord-275413-e2rhioty 279 13 infection infection NN cord-275413-e2rhioty 279 14 . . . cord-275413-e2rhioty 280 1 Up up RB cord-275413-e2rhioty 280 2 - - HYPH cord-275413-e2rhioty 280 3 regulated regulate VBN cord-275413-e2rhioty 280 4 expression expression NN cord-275413-e2rhioty 280 5 was be VBD cord-275413-e2rhioty 280 6 confirmed confirm VBN cord-275413-e2rhioty 280 7 by by IN cord-275413-e2rhioty 280 8 the the DT cord-275413-e2rhioty 280 9 presence presence NN cord-275413-e2rhioty 280 10 of of IN cord-275413-e2rhioty 280 11 detectable detectable JJ cord-275413-e2rhioty 280 12 levels level NNS cord-275413-e2rhioty 280 13 of of IN cord-275413-e2rhioty 280 14 IFN- IFN- NNP cord-275413-e2rhioty 280 15 ␥ ␥ CD cord-275413-e2rhioty 280 16 and and CC cord-275413-e2rhioty 280 17 TNF- TNF- NNP cord-275413-e2rhioty 280 18 ␣ ␣ NN cord-275413-e2rhioty 280 19 proteins protein NNS cord-275413-e2rhioty 280 20 in in IN cord-275413-e2rhioty 280 21 serum serum NN cord-275413-e2rhioty 280 22 . . . cord-275413-e2rhioty 281 1 Furthermore furthermore RB cord-275413-e2rhioty 281 2 , , , cord-275413-e2rhioty 281 3 the the DT cord-275413-e2rhioty 281 4 results result NNS cord-275413-e2rhioty 281 5 indicate indicate VBP cord-275413-e2rhioty 281 6 that that IN cord-275413-e2rhioty 281 7 INF- INF- NNP cord-275413-e2rhioty 282 1 ␣ ␣ NNP cord-275413-e2rhioty 282 2 and and CC cord-275413-e2rhioty 283 1 IFN- IFN- NNP cord-275413-e2rhioty 283 2 ␥ ␥ CD cord-275413-e2rhioty 283 3 cytokines cytokine NNS cord-275413-e2rhioty 283 4 response response NN cord-275413-e2rhioty 283 5 are be VBP cord-275413-e2rhioty 283 6 compartmentalized compartmentalize VBN cord-275413-e2rhioty 283 7 within within IN cord-275413-e2rhioty 283 8 the the DT cord-275413-e2rhioty 283 9 fetus fetus NN cord-275413-e2rhioty 283 10 and and CC cord-275413-e2rhioty 283 11 do do VBP cord-275413-e2rhioty 283 12 not not RB cord-275413-e2rhioty 283 13 extend extend VB cord-275413-e2rhioty 283 14 to to IN cord-275413-e2rhioty 283 15 other other JJ cord-275413-e2rhioty 283 16 compartments compartment NNS cord-275413-e2rhioty 283 17 , , , cord-275413-e2rhioty 283 18 such such JJ cord-275413-e2rhioty 283 19 as as IN cord-275413-e2rhioty 283 20 amniotic amniotic NN cord-275413-e2rhioty 283 21 fluid fluid NN cord-275413-e2rhioty 283 22 or or CC cord-275413-e2rhioty 283 23 placenta placenta NN cord-275413-e2rhioty 283 24 , , , cord-275413-e2rhioty 283 25 thus thus RB cord-275413-e2rhioty 283 26 lessening lessen VBG cord-275413-e2rhioty 283 27 the the DT cord-275413-e2rhioty 283 28 probability probability NN cord-275413-e2rhioty 283 29 of of IN cord-275413-e2rhioty 283 30 allorejection allorejection NN cord-275413-e2rhioty 283 31 by by IN cord-275413-e2rhioty 283 32 the the DT cord-275413-e2rhioty 283 33 dam dam NN cord-275413-e2rhioty 283 34 . . . cord-275413-e2rhioty 284 1 From from IN cord-275413-e2rhioty 284 2 these these DT cord-275413-e2rhioty 284 3 data datum NNS cord-275413-e2rhioty 284 4 , , , cord-275413-e2rhioty 284 5 it -PRON- PRP cord-275413-e2rhioty 284 6 is be VBZ cord-275413-e2rhioty 284 7 apparent apparent JJ cord-275413-e2rhioty 284 8 that that IN cord-275413-e2rhioty 284 9 the the DT cord-275413-e2rhioty 284 10 fetus fetus NN cord-275413-e2rhioty 284 11 is be VBZ cord-275413-e2rhioty 284 12 capable capable JJ cord-275413-e2rhioty 284 13 of of IN cord-275413-e2rhioty 284 14 initiating initiate VBG cord-275413-e2rhioty 284 15 a a DT cord-275413-e2rhioty 284 16 Th1-like Th1-like NNP cord-275413-e2rhioty 284 17 response response NN cord-275413-e2rhioty 284 18 ; ; : cord-275413-e2rhioty 284 19 however however RB cord-275413-e2rhioty 284 20 , , , cord-275413-e2rhioty 284 21 the the DT cord-275413-e2rhioty 284 22 capacity capacity NN cord-275413-e2rhioty 284 23 of of IN cord-275413-e2rhioty 285 1 IFN- IFN- NNP cord-275413-e2rhioty 285 2 ␥ ␥ CD cord-275413-e2rhioty 285 3 and and CC cord-275413-e2rhioty 285 4 TNF- TNF- NNP cord-275413-e2rhioty 285 5 ␣ ␣ NNP cord-275413-e2rhioty 285 6 to to TO cord-275413-e2rhioty 285 7 control control VB cord-275413-e2rhioty 285 8 PRRSV PRRSV NNP cord-275413-e2rhioty 285 9 infection infection NN cord-275413-e2rhioty 285 10 in in IN cord-275413-e2rhioty 285 11 the the DT cord-275413-e2rhioty 285 12 fetus fetus NN cord-275413-e2rhioty 285 13 is be VBZ cord-275413-e2rhioty 285 14 not not RB cord-275413-e2rhioty 285 15 known know VBN cord-275413-e2rhioty 285 16 . . . cord-275413-e2rhioty 286 1 Additional additional JJ cord-275413-e2rhioty 286 2 evidence evidence NN cord-275413-e2rhioty 286 3 for for IN cord-275413-e2rhioty 286 4 induction induction NN cord-275413-e2rhioty 286 5 of of IN cord-275413-e2rhioty 286 6 virus virus NN cord-275413-e2rhioty 286 7 - - HYPH cord-275413-e2rhioty 286 8 specific specific JJ cord-275413-e2rhioty 286 9 immunity immunity NN cord-275413-e2rhioty 286 10 was be VBD cord-275413-e2rhioty 286 11 found find VBN cord-275413-e2rhioty 286 12 in in IN cord-275413-e2rhioty 286 13 the the DT cord-275413-e2rhioty 286 14 development development NN cord-275413-e2rhioty 286 15 of of IN cord-275413-e2rhioty 286 16 germinal germinal JJ cord-275413-e2rhioty 286 17 centers center NNS cord-275413-e2rhioty 286 18 containing contain VBG cord-275413-e2rhioty 286 19 activated activate VBN cord-275413-e2rhioty 286 20 ( ( -LRB- cord-275413-e2rhioty 286 21 CDw75 CDw75 NNS cord-275413-e2rhioty 286 22 + + CC cord-275413-e2rhioty 286 23 ) ) -RRB- cord-275413-e2rhioty 286 24 B b NN cord-275413-e2rhioty 286 25 cells cell NNS cord-275413-e2rhioty 286 26 and and CC cord-275413-e2rhioty 286 27 seroconversion seroconversion NN cord-275413-e2rhioty 286 28 in in IN cord-275413-e2rhioty 286 29 at at RB cord-275413-e2rhioty 286 30 least least RBS cord-275413-e2rhioty 286 31 one one CD cord-275413-e2rhioty 286 32 fetus fetus NN cord-275413-e2rhioty 286 33 . . . cord-275413-e2rhioty 287 1 Pro pro JJ cord-275413-e2rhioty 287 2 - - JJ cord-275413-e2rhioty 287 3 inflammatory inflammatory JJ cord-275413-e2rhioty 287 4 cytokines cytokine NNS cord-275413-e2rhioty 287 5 , , , cord-275413-e2rhioty 287 6 such such JJ cord-275413-e2rhioty 287 7 as as IN cord-275413-e2rhioty 287 8 TNF- TNF- NNP cord-275413-e2rhioty 287 9 ␣ ␣ NNP cord-275413-e2rhioty 287 10 and and CC cord-275413-e2rhioty 287 11 IFN- IFN- NNP cord-275413-e2rhioty 287 12 ␥ ␥ NNP cord-275413-e2rhioty 287 13 can can MD cord-275413-e2rhioty 287 14 contribute contribute VB cord-275413-e2rhioty 287 15 to to IN cord-275413-e2rhioty 287 16 pulmonary pulmonary JJ cord-275413-e2rhioty 287 17 distress distress NN cord-275413-e2rhioty 287 18 through through IN cord-275413-e2rhioty 287 19 the the DT cord-275413-e2rhioty 287 20 activation activation NN cord-275413-e2rhioty 287 21 of of IN cord-275413-e2rhioty 287 22 alveolar alveolar JJ cord-275413-e2rhioty 287 23 macrophages macrophage NNS cord-275413-e2rhioty 287 24 and and CC cord-275413-e2rhioty 287 25 other other JJ cord-275413-e2rhioty 287 26 cell cell NN cord-275413-e2rhioty 287 27 populations population NNS cord-275413-e2rhioty 287 28 . . . cord-275413-e2rhioty 288 1 The the DT cord-275413-e2rhioty 288 2 increased increase VBN cord-275413-e2rhioty 288 3 quantities quantity NNS cord-275413-e2rhioty 288 4 of of IN cord-275413-e2rhioty 289 1 IFN- IFN- NNP cord-275413-e2rhioty 289 2 ␥ ␥ CD cord-275413-e2rhioty 289 3 and and CC cord-275413-e2rhioty 289 4 TNF- TNF- NNP cord-275413-e2rhioty 289 5 ␣ ␣ NNP cord-275413-e2rhioty 289 6 found find VBD cord-275413-e2rhioty 289 7 in in IN cord-275413-e2rhioty 289 8 the the DT cord-275413-e2rhioty 289 9 lungs lung NNS cord-275413-e2rhioty 289 10 of of IN cord-275413-e2rhioty 289 11 PRRSV PRRSV NNP cord-275413-e2rhioty 289 12 - - HYPH cord-275413-e2rhioty 289 13 infected infect VBN cord-275413-e2rhioty 289 14 fetuses fetus NNS cord-275413-e2rhioty 289 15 may may MD cord-275413-e2rhioty 289 16 not not RB cord-275413-e2rhioty 289 17 be be VB cord-275413-e2rhioty 289 18 sufficient sufficient JJ cord-275413-e2rhioty 289 19 to to TO cord-275413-e2rhioty 289 20 cause cause VB cord-275413-e2rhioty 289 21 pulmonary pulmonary JJ cord-275413-e2rhioty 289 22 damage damage NN cord-275413-e2rhioty 289 23 to to IN cord-275413-e2rhioty 289 24 the the DT cord-275413-e2rhioty 289 25 fetal fetal JJ cord-275413-e2rhioty 289 26 lung lung NN cord-275413-e2rhioty 289 27 , , , cord-275413-e2rhioty 289 28 primarily primarily RB cord-275413-e2rhioty 289 29 because because IN cord-275413-e2rhioty 289 30 fetal fetal JJ cord-275413-e2rhioty 289 31 macrophages macrophage NNS cord-275413-e2rhioty 289 32 have have VBP cord-275413-e2rhioty 289 33 a a DT cord-275413-e2rhioty 289 34 reduced reduce VBN cord-275413-e2rhioty 289 35 capacity capacity NN cord-275413-e2rhioty 289 36 to to TO cord-275413-e2rhioty 289 37 respond respond VB cord-275413-e2rhioty 289 38 to to IN cord-275413-e2rhioty 289 39 inflammatory inflammatory JJ cord-275413-e2rhioty 289 40 stimuli stimulus NNS cord-275413-e2rhioty 289 41 . . . cord-275413-e2rhioty 290 1 In in IN cord-275413-e2rhioty 290 2 addition addition NN cord-275413-e2rhioty 290 3 , , , cord-275413-e2rhioty 290 4 IL-10 IL-10 NNP cord-275413-e2rhioty 290 5 , , , cord-275413-e2rhioty 290 6 up up RB cord-275413-e2rhioty 290 7 - - HYPH cord-275413-e2rhioty 290 8 regulated regulate VBN cord-275413-e2rhioty 290 9 in in IN cord-275413-e2rhioty 290 10 response response NN cord-275413-e2rhioty 290 11 to to IN cord-275413-e2rhioty 290 12 PRRSV PRRSV NNP cord-275413-e2rhioty 290 13 infection infection NN cord-275413-e2rhioty 290 14 , , , cord-275413-e2rhioty 290 15 is be VBZ cord-275413-e2rhioty 290 16 a a DT cord-275413-e2rhioty 290 17 potent potent JJ cord-275413-e2rhioty 290 18 antagonist antagonist NN cord-275413-e2rhioty 290 19 of of IN cord-275413-e2rhioty 291 1 IFN- IFN- NNP cord-275413-e2rhioty 291 2 ␥ ␥ CD cord-275413-e2rhioty 291 3 and and CC cord-275413-e2rhioty 291 4 TNF- TNF- NNP cord-275413-e2rhioty 291 5 ␣ ␣ NN cord-275413-e2rhioty 291 6 activation activation NN cord-275413-e2rhioty 291 7 of of IN cord-275413-e2rhioty 291 8 macrophages macrophage NNS cord-275413-e2rhioty 291 9 ( ( -LRB- cord-275413-e2rhioty 291 10 Burchett Burchett NNP cord-275413-e2rhioty 291 11 et et FW cord-275413-e2rhioty 291 12 al al NNP cord-275413-e2rhioty 291 13 . . NNP cord-275413-e2rhioty 291 14 , , , cord-275413-e2rhioty 291 15 1992 1992 CD cord-275413-e2rhioty 291 16 ; ; : cord-275413-e2rhioty 291 17 Marodi Marodi NNP cord-275413-e2rhioty 291 18 et et NNP cord-275413-e2rhioty 291 19 al al NNP cord-275413-e2rhioty 291 20 . . . cord-275413-e2rhioty 292 1 , , , cord-275413-e2rhioty 292 2 2001 2001 CD cord-275413-e2rhioty 292 3 ; ; : cord-275413-e2rhioty 292 4 Johnsen Johnsen NNP cord-275413-e2rhioty 292 5 et et NNP cord-275413-e2rhioty 292 6 al al NNP cord-275413-e2rhioty 292 7 . . . cord-275413-e2rhioty 293 1 , , , cord-275413-e2rhioty 293 2 2002 2002 CD cord-275413-e2rhioty 293 3 ; ; : cord-275413-e2rhioty 293 4 Thanawongnuwech Thanawongnuwech NNP cord-275413-e2rhioty 293 5 et et NNP cord-275413-e2rhioty 293 6 al al NNP cord-275413-e2rhioty 293 7 . . NNP cord-275413-e2rhioty 293 8 , , , cord-275413-e2rhioty 293 9 2003 2003 CD cord-275413-e2rhioty 293 10 ) ) -RRB- cord-275413-e2rhioty 293 11 . . . cord-275413-e2rhioty 294 1 However however RB cord-275413-e2rhioty 294 2 , , , cord-275413-e2rhioty 294 3 within within IN cord-275413-e2rhioty 294 4 days day NNS cord-275413-e2rhioty 294 5 after after IN cord-275413-e2rhioty 294 6 birth birth NN cord-275413-e2rhioty 294 7 , , , cord-275413-e2rhioty 294 8 adult adult NN cord-275413-e2rhioty 294 9 macrophages macrophage NNS cord-275413-e2rhioty 294 10 , , , cord-275413-e2rhioty 294 11 including include VBG cord-275413-e2rhioty 294 12 mature mature JJ cord-275413-e2rhioty 294 13 alveolar alveolar NN cord-275413-e2rhioty 294 14 and and CC cord-275413-e2rhioty 294 15 intravascular intravascular JJ cord-275413-e2rhioty 294 16 macrophages macrophage NNS cord-275413-e2rhioty 294 17 emerge emerge VBP cord-275413-e2rhioty 294 18 into into IN cord-275413-e2rhioty 294 19 an an DT cord-275413-e2rhioty 294 20 environment environment NN cord-275413-e2rhioty 294 21 already already RB cord-275413-e2rhioty 294 22 enriched enrich VBN cord-275413-e2rhioty 294 23 in in IN cord-275413-e2rhioty 294 24 IFN- IFN- NNP cord-275413-e2rhioty 294 25 ␥ ␥ NNP cord-275413-e2rhioty 294 26 and and CC cord-275413-e2rhioty 294 27 TNF- TNF- NNP cord-275413-e2rhioty 294 28 ␣ ␣ NN cord-275413-e2rhioty 294 29 . . . cord-275413-e2rhioty 295 1 The the DT cord-275413-e2rhioty 295 2 outcome outcome NN cord-275413-e2rhioty 295 3 is be VBZ cord-275413-e2rhioty 295 4 the the DT cord-275413-e2rhioty 295 5 rapid rapid JJ cord-275413-e2rhioty 295 6 recruitment recruitment NN cord-275413-e2rhioty 295 7 , , , cord-275413-e2rhioty 295 8 activation activation NN cord-275413-e2rhioty 295 9 , , , cord-275413-e2rhioty 295 10 and and CC cord-275413-e2rhioty 295 11 cytopathic cytopathic JJ cord-275413-e2rhioty 295 12 killing killing NN cord-275413-e2rhioty 295 13 of of IN cord-275413-e2rhioty 295 14 large large JJ cord-275413-e2rhioty 295 15 numbers number NNS cord-275413-e2rhioty 295 16 of of IN cord-275413-e2rhioty 295 17 virus virus NN cord-275413-e2rhioty 295 18 - - HYPH cord-275413-e2rhioty 295 19 permissive permissive JJ cord-275413-e2rhioty 295 20 macrophages macrophage NNS cord-275413-e2rhioty 295 21 ( ( -LRB- cord-275413-e2rhioty 295 22 Choi Choi NNP cord-275413-e2rhioty 295 23 and and CC cord-275413-e2rhioty 295 24 Chae Chae NNP cord-275413-e2rhioty 295 25 , , , cord-275413-e2rhioty 295 26 2002 2002 CD cord-275413-e2rhioty 295 27 ) ) -RRB- cord-275413-e2rhioty 295 28 . . . cord-275413-e2rhioty 296 1 This this DT cord-275413-e2rhioty 296 2 scenario scenario NN cord-275413-e2rhioty 296 3 as as IN cord-275413-e2rhioty 296 4 a a DT cord-275413-e2rhioty 296 5 cause cause NN cord-275413-e2rhioty 296 6 of of IN cord-275413-e2rhioty 296 7 severe severe JJ cord-275413-e2rhioty 296 8 interstitial interstitial JJ cord-275413-e2rhioty 296 9 pnuemonia pnuemonia NN cord-275413-e2rhioty 296 10 in in IN cord-275413-e2rhioty 296 11 the the DT cord-275413-e2rhioty 296 12 PRRSV PRRSV NNP cord-275413-e2rhioty 296 13 - - HYPH cord-275413-e2rhioty 296 14 infected infect VBN cord-275413-e2rhioty 296 15 newborn newborn NN cord-275413-e2rhioty 296 16 requires require VBZ cord-275413-e2rhioty 296 17 further further JJ cord-275413-e2rhioty 296 18 investigation investigation NN cord-275413-e2rhioty 296 19 , , , cord-275413-e2rhioty 296 20 but but CC cord-275413-e2rhioty 296 21 has have VBZ cord-275413-e2rhioty 296 22 obvious obvious JJ cord-275413-e2rhioty 296 23 implications implication NNS cord-275413-e2rhioty 296 24 in in IN cord-275413-e2rhioty 296 25 the the DT cord-275413-e2rhioty 296 26 etiology etiology NN cord-275413-e2rhioty 296 27 of of IN cord-275413-e2rhioty 296 28 postnatal postnatal JJ cord-275413-e2rhioty 296 29 pulmonary pulmonary JJ cord-275413-e2rhioty 296 30 complications complication NNS cord-275413-e2rhioty 296 31 following follow VBG cord-275413-e2rhioty 296 32 virus virus NN cord-275413-e2rhioty 296 33 infections infection NNS cord-275413-e2rhioty 296 34 of of IN cord-275413-e2rhioty 296 35 the the DT cord-275413-e2rhioty 296 36 fetus fetus NN cord-275413-e2rhioty 296 37 . . . cord-275413-e2rhioty 297 1 Porcine porcine JJ cord-275413-e2rhioty 297 2 reproductive reproductive JJ cord-275413-e2rhioty 297 3 and and CC cord-275413-e2rhioty 297 4 respiratory respiratory JJ cord-275413-e2rhioty 297 5 syndrome syndrome NN cord-275413-e2rhioty 297 6 virus virus NN cord-275413-e2rhioty 297 7 : : : cord-275413-e2rhioty 297 8 description description NN cord-275413-e2rhioty 297 9 of of IN cord-275413-e2rhioty 297 10 persistence persistence NN cord-275413-e2rhioty 297 11 in in IN cord-275413-e2rhioty 297 12 individual individual JJ cord-275413-e2rhioty 297 13 pigs pig NNS cord-275413-e2rhioty 297 14 upon upon IN cord-275413-e2rhioty 297 15 experimental experimental JJ cord-275413-e2rhioty 297 16 infection infection NN cord-275413-e2rhioty 297 17 Murine Murine NNP cord-275413-e2rhioty 297 18 T T NNP cord-275413-e2rhioty 297 19 cell cell NN cord-275413-e2rhioty 297 20 determination determination NN cord-275413-e2rhioty 297 21 of of IN cord-275413-e2rhioty 297 22 pregnancy pregnancy NN cord-275413-e2rhioty 297 23 outcome outcome NN cord-275413-e2rhioty 297 24 Maternal maternal JJ cord-275413-e2rhioty 297 25 and and CC cord-275413-e2rhioty 297 26 foetal foetal JJ cord-275413-e2rhioty 297 27 consequences consequence NNS cord-275413-e2rhioty 297 28 of of IN cord-275413-e2rhioty 297 29 dengue dengue NN cord-275413-e2rhioty 297 30 fever fever NN cord-275413-e2rhioty 297 31 during during IN cord-275413-e2rhioty 297 32 pregnancy pregnancy NN cord-275413-e2rhioty 297 33 Characterization characterization NN cord-275413-e2rhioty 297 34 of of IN cord-275413-e2rhioty 297 35 swine swine NN cord-275413-e2rhioty 297 36 infertility infertility NN cord-275413-e2rhioty 297 37 and and CC cord-275413-e2rhioty 297 38 respiratory respiratory JJ cord-275413-e2rhioty 297 39 syndrome syndrome NN cord-275413-e2rhioty 297 40 ( ( -LRB- cord-275413-e2rhioty 297 41 SIRS SIRS NNP cord-275413-e2rhioty 297 42 ) ) -RRB- cord-275413-e2rhioty 297 43 virus virus NN cord-275413-e2rhioty 297 44 ( ( -LRB- cord-275413-e2rhioty 297 45 Isolate isolate VB cord-275413-e2rhioty 297 46 ATCC ATCC NNP cord-275413-e2rhioty 297 47 VR-2332 vr-2332 NN cord-275413-e2rhioty 297 48 ) ) -RRB- cord-275413-e2rhioty 298 1 Porcine porcine JJ cord-275413-e2rhioty 298 2 reproductive reproductive JJ cord-275413-e2rhioty 298 3 and and CC cord-275413-e2rhioty 298 4 respiratory respiratory JJ cord-275413-e2rhioty 298 5 syndrome syndrome NN cord-275413-e2rhioty 298 6 virus virus NN cord-275413-e2rhioty 299 1 A a DT cord-275413-e2rhioty 299 2 comparison comparison NN cord-275413-e2rhioty 299 3 of of IN cord-275413-e2rhioty 299 4 virus virus NN cord-275413-e2rhioty 299 5 isolation isolation NN cord-275413-e2rhioty 299 6 , , , cord-275413-e2rhioty 299 7 immunohistochemistry immunohistochemistry NN cord-275413-e2rhioty 299 8 , , , cord-275413-e2rhioty 299 9 fetal fetal JJ cord-275413-e2rhioty 299 10 serology serology NN cord-275413-e2rhioty 299 11 , , , cord-275413-e2rhioty 299 12 and and CC cord-275413-e2rhioty 299 13 reverse reverse JJ cord-275413-e2rhioty 299 14 - - HYPH cord-275413-e2rhioty 299 15 transcription transcription NN cord-275413-e2rhioty 299 16 polymerase polymerase NN cord-275413-e2rhioty 299 17 chain chain NN cord-275413-e2rhioty 299 18 reaction reaction NN cord-275413-e2rhioty 299 19 assay assay NN cord-275413-e2rhioty 299 20 for for IN cord-275413-e2rhioty 299 21 identification identification NN cord-275413-e2rhioty 299 22 of of IN cord-275413-e2rhioty 299 23 porcine porcine JJ cord-275413-e2rhioty 299 24 reproductive reproductive JJ cord-275413-e2rhioty 299 25 and and CC cord-275413-e2rhioty 299 26 respiratory respiratory JJ cord-275413-e2rhioty 299 27 syndrome syndrome NN cord-275413-e2rhioty 299 28 virus virus NN cord-275413-e2rhioty 299 29 transplacental transplacental NNP cord-275413-e2rhioty 299 30 infection infection NN cord-275413-e2rhioty 299 31 in in IN cord-275413-e2rhioty 299 32 the the DT cord-275413-e2rhioty 299 33 fetus fetus NN cord-275413-e2rhioty 299 34 Diminished diminish VBN cord-275413-e2rhioty 299 35 interferon interferon NN cord-275413-e2rhioty 299 36 - - HYPH cord-275413-e2rhioty 299 37 gamma gamma NN cord-275413-e2rhioty 299 38 and and CC cord-275413-e2rhioty 299 39 lymphocyte lymphocyte NN cord-275413-e2rhioty 299 40 proliferation proliferation NN cord-275413-e2rhioty 299 41 in in IN cord-275413-e2rhioty 299 42 neonatal neonatal JJ cord-275413-e2rhioty 299 43 and and CC cord-275413-e2rhioty 299 44 postpartum postpartum NN cord-275413-e2rhioty 299 45 primary primary JJ cord-275413-e2rhioty 299 46 herpes herpes NN cord-275413-e2rhioty 299 47 simplex simplex NN cord-275413-e2rhioty 299 48 virus virus NN cord-275413-e2rhioty 299 49 infection infection NN cord-275413-e2rhioty 299 50 Trojan Trojan NNP cord-275413-e2rhioty 299 51 Horse Horse NNP cord-275413-e2rhioty 299 52 macrophages macrophage NNS cord-275413-e2rhioty 299 53 : : : cord-275413-e2rhioty 299 54 studies study NNS cord-275413-e2rhioty 299 55 with with IN cord-275413-e2rhioty 299 56 the the DT cord-275413-e2rhioty 299 57 murine murine JJ cord-275413-e2rhioty 299 58 lactate lactate NN cord-275413-e2rhioty 299 59 dehydrogenase dehydrogenase NN cord-275413-e2rhioty 299 60 - - HYPH cord-275413-e2rhioty 299 61 elevating elevate VBG cord-275413-e2rhioty 299 62 virus virus NN cord-275413-e2rhioty 299 63 and and CC cord-275413-e2rhioty 299 64 implications implication NNS cord-275413-e2rhioty 299 65 for for IN cord-275413-e2rhioty 299 66 sexually sexually RB cord-275413-e2rhioty 299 67 transmitted transmit VBN cord-275413-e2rhioty 299 68 virus virus NN cord-275413-e2rhioty 299 69 infection infection NN cord-275413-e2rhioty 299 70 Nidovirales Nidovirales NNP cord-275413-e2rhioty 299 71 . . . cord-275413-e2rhioty 300 1 A a DT cord-275413-e2rhioty 300 2 new new JJ cord-275413-e2rhioty 300 3 order order NN cord-275413-e2rhioty 300 4 comprising comprise VBG cord-275413-e2rhioty 300 5 Coronaviridae Coronaviridae NNP cord-275413-e2rhioty 300 6 and and CC cord-275413-e2rhioty 300 7 Arterivirdae Arterivirdae NNP cord-275413-e2rhioty 300 8 Inflammation Inflammation NNP cord-275413-e2rhioty 300 9 and and CC cord-275413-e2rhioty 300 10 pregnancy pregnancy NN cord-275413-e2rhioty 300 11 A a DT cord-275413-e2rhioty 300 12 brief brief JJ cord-275413-e2rhioty 300 13 review review NN cord-275413-e2rhioty 300 14 of of IN cord-275413-e2rhioty 300 15 recent recent JJ cord-275413-e2rhioty 300 16 data datum NNS cord-275413-e2rhioty 300 17 on on IN cord-275413-e2rhioty 300 18 some some DT cord-275413-e2rhioty 300 19 cytokine cytokine JJ cord-275413-e2rhioty 300 20 expressions expression NNS cord-275413-e2rhioty 300 21 at at IN cord-275413-e2rhioty 300 22 the the DT cord-275413-e2rhioty 300 23 materno materno NN cord-275413-e2rhioty 300 24 - - HYPH cord-275413-e2rhioty 300 25 foetal foetal JJ cord-275413-e2rhioty 300 26 interface interface NN cord-275413-e2rhioty 300 27 which which WDT cord-275413-e2rhioty 300 28 might may MD cord-275413-e2rhioty 300 29 challenge challenge VB cord-275413-e2rhioty 300 30 the the DT cord-275413-e2rhioty 300 31 classical classical JJ cord-275413-e2rhioty 300 32 Th1 Th1 NNP cord-275413-e2rhioty 300 33 / / SYM cord-275413-e2rhioty 300 34 Th2 Th2 NNP cord-275413-e2rhioty 300 35 dichotomy dichotomy NN cord-275413-e2rhioty 300 36 Control Control NNP cord-275413-e2rhioty 300 37 of of IN cord-275413-e2rhioty 300 38 fetal fetal JJ cord-275413-e2rhioty 300 39 survival survival NN cord-275413-e2rhioty 300 40 in in IN cord-275413-e2rhioty 300 41 CBA CBA NNP cord-275413-e2rhioty 300 42 × × NNP cord-275413-e2rhioty 301 1 DBA/2 DBA/2 NNP cord-275413-e2rhioty 301 2 mice mouse NNS cord-275413-e2rhioty 301 3 by by IN cord-275413-e2rhioty 301 4 lymphokine lymphokine NN cord-275413-e2rhioty 301 5 therapy therapy NN cord-275413-e2rhioty 301 6 Neonatal neonatal NN cord-275413-e2rhioty 301 7 ( ( -LRB- cord-275413-e2rhioty 301 8 cord cord NN cord-275413-e2rhioty 301 9 blood blood NN cord-275413-e2rhioty 301 10 ) ) -RRB- cord-275413-e2rhioty 302 1 T t NN cord-275413-e2rhioty 302 2 cells cell NNS cord-275413-e2rhioty 302 3 can can MD cord-275413-e2rhioty 302 4 competently competently RB cord-275413-e2rhioty 302 5 raise raise VB cord-275413-e2rhioty 302 6 type type NN cord-275413-e2rhioty 302 7 1 1 CD cord-275413-e2rhioty 302 8 and and CC cord-275413-e2rhioty 302 9 2 2 CD cord-275413-e2rhioty 302 10 immune immune JJ cord-275413-e2rhioty 302 11 responses response NNS cord-275413-e2rhioty 302 12 upon upon IN cord-275413-e2rhioty 302 13 polyclonal polyclonal JJ cord-275413-e2rhioty 302 14 activation activation NN cord-275413-e2rhioty 302 15 Expression expression NN cord-275413-e2rhioty 302 16 of of IN cord-275413-e2rhioty 302 17 tumour tumour NN cord-275413-e2rhioty 302 18 necrosis necrosis NN cord-275413-e2rhioty 302 19 factor factor NN cord-275413-e2rhioty 302 20 - - HYPH cord-275413-e2rhioty 302 21 alpha alpha NN cord-275413-e2rhioty 302 22 is be VBZ cord-275413-e2rhioty 302 23 associated associate VBN cord-275413-e2rhioty 302 24 with with IN cord-275413-e2rhioty 302 25 apoptosis apoptosis NN cord-275413-e2rhioty 302 26 in in IN cord-275413-e2rhioty 302 27 lungs lung NNS cord-275413-e2rhioty 302 28 of of IN cord-275413-e2rhioty 302 29 pigs pig NNS cord-275413-e2rhioty 302 30 experimentally experimentally RB cord-275413-e2rhioty 302 31 infected infect VBN cord-275413-e2rhioty 302 32 with with IN cord-275413-e2rhioty 302 33 porcine porcine JJ cord-275413-e2rhioty 302 34 reproductive reproductive JJ cord-275413-e2rhioty 302 35 and and CC cord-275413-e2rhioty 302 36 respiratory respiratory JJ cord-275413-e2rhioty 302 37 syndrome syndrome NN cord-275413-e2rhioty 302 38 virus virus NN cord-275413-e2rhioty 302 39 Pathogenesis Pathogenesis NNP cord-275413-e2rhioty 302 40 of of IN cord-275413-e2rhioty 302 41 porcine porcine JJ cord-275413-e2rhioty 302 42 reproductive reproductive JJ cord-275413-e2rhioty 302 43 and and CC cord-275413-e2rhioty 302 44 respiratory respiratory JJ cord-275413-e2rhioty 302 45 syndrome syndrome NN cord-275413-e2rhioty 302 46 virus virus NN cord-275413-e2rhioty 302 47 infection infection NN cord-275413-e2rhioty 302 48 in in IN cord-275413-e2rhioty 302 49 mid mid JJ cord-275413-e2rhioty 302 50 gestation gestation NN cord-275413-e2rhioty 302 51 sows sow NNS cord-275413-e2rhioty 302 52 and and CC cord-275413-e2rhioty 302 53 fetuses fetuse VBZ cord-275413-e2rhioty 302 54 Experimental experimental JJ cord-275413-e2rhioty 302 55 reproduction reproduction NN cord-275413-e2rhioty 302 56 of of IN cord-275413-e2rhioty 302 57 swine swine NN cord-275413-e2rhioty 302 58 infertility infertility NN cord-275413-e2rhioty 302 59 and and CC cord-275413-e2rhioty 302 60 respiratory respiratory JJ cord-275413-e2rhioty 302 61 syndrome syndrome NN cord-275413-e2rhioty 302 62 in in IN cord-275413-e2rhioty 302 63 pregnant pregnant JJ cord-275413-e2rhioty 302 64 sows sow NNS cord-275413-e2rhioty 302 65 Ecology Ecology NNP cord-275413-e2rhioty 302 66 of of IN cord-275413-e2rhioty 302 67 danger danger NN cord-275413-e2rhioty 302 68 - - HYPH cord-275413-e2rhioty 302 69 dependent dependent JJ cord-275413-e2rhioty 302 70 cytokine cytokine NN cord-275413-e2rhioty 302 71 - - HYPH cord-275413-e2rhioty 302 72 boosted boost VBN cord-275413-e2rhioty 302 73 spontaneous spontaneous JJ cord-275413-e2rhioty 302 74 abortion abortion NN cord-275413-e2rhioty 302 75 in in IN cord-275413-e2rhioty 302 76 the the DT cord-275413-e2rhioty 302 77 CBA CBA NNP cord-275413-e2rhioty 302 78 x x SYM cord-275413-e2rhioty 302 79 DBA/2 DBA/2 NNP cord-275413-e2rhioty 302 80 mouse mouse NN cord-275413-e2rhioty 302 81 model model NN cord-275413-e2rhioty 302 82 . . . cord-275413-e2rhioty 303 1 I. I. NNP cord-275413-e2rhioty 303 2 Synergistic synergistic JJ cord-275413-e2rhioty 303 3 effect effect NN cord-275413-e2rhioty 303 4 of of IN cord-275413-e2rhioty 303 5 LPS LPS NNP cord-275413-e2rhioty 303 6 and and CC cord-275413-e2rhioty 303 7 ( ( -LRB- cord-275413-e2rhioty 303 8 TNF tnf NN cord-275413-e2rhioty 303 9 - - HYPH cord-275413-e2rhioty 303 10 alpha alpha NN cord-275413-e2rhioty 304 1 + + CC cord-275413-e2rhioty 304 2 IFN IFN NNP cord-275413-e2rhioty 304 3 - - HYPH cord-275413-e2rhioty 304 4 gamma gamma NNP cord-275413-e2rhioty 304 5 ) ) -RRB- cord-275413-e2rhioty 304 6 on on IN cord-275413-e2rhioty 304 7 pregnancy pregnancy NN cord-275413-e2rhioty 304 8 loss loss NN cord-275413-e2rhioty 304 9 Pathology Pathology NNP cord-275413-e2rhioty 304 10 of of IN cord-275413-e2rhioty 304 11 maternal maternal JJ cord-275413-e2rhioty 304 12 genital genital JJ cord-275413-e2rhioty 304 13 tract tract NN cord-275413-e2rhioty 304 14 , , , cord-275413-e2rhioty 304 15 placenta placenta NN cord-275413-e2rhioty 304 16 , , , cord-275413-e2rhioty 304 17 and and CC cord-275413-e2rhioty 304 18 fetus fetus NN cord-275413-e2rhioty 304 19 in in IN cord-275413-e2rhioty 304 20 equine equine JJ cord-275413-e2rhioty 304 21 viral viral JJ cord-275413-e2rhioty 304 22 arteritis arteritis NN cord-275413-e2rhioty 304 23 Isolation isolation NN cord-275413-e2rhioty 304 24 of of IN cord-275413-e2rhioty 304 25 swine swine NN cord-275413-e2rhioty 304 26 infertility infertility NN cord-275413-e2rhioty 304 27 and and CC cord-275413-e2rhioty 304 28 respiratory respiratory JJ cord-275413-e2rhioty 304 29 syndrome syndrome NN cord-275413-e2rhioty 304 30 virus virus NN cord-275413-e2rhioty 304 31 ( ( -LRB- cord-275413-e2rhioty 304 32 isolate isolate VB cord-275413-e2rhioty 304 33 ATCC ATCC NNP cord-275413-e2rhioty 304 34 VR-2332 vr-2332 NN cord-275413-e2rhioty 304 35 ) ) -RRB- cord-275413-e2rhioty 304 36 in in IN cord-275413-e2rhioty 304 37 North North NNP cord-275413-e2rhioty 304 38 America America NNP cord-275413-e2rhioty 304 39 and and CC cord-275413-e2rhioty 304 40 experimental experimental JJ cord-275413-e2rhioty 304 41 reproduction reproduction NN cord-275413-e2rhioty 304 42 of of IN cord-275413-e2rhioty 304 43 the the DT cord-275413-e2rhioty 304 44 disease disease NN cord-275413-e2rhioty 304 45 in in IN cord-275413-e2rhioty 304 46 gnotobiotic gnotobiotic JJ cord-275413-e2rhioty 304 47 pigs pig NNS cord-275413-e2rhioty 305 1 The the DT cord-275413-e2rhioty 305 2 two two CD cord-275413-e2rhioty 305 3 faces face NNS cord-275413-e2rhioty 305 4 of of IN cord-275413-e2rhioty 305 5 mutation mutation NN cord-275413-e2rhioty 305 6 : : : cord-275413-e2rhioty 305 7 extinction extinction NN cord-275413-e2rhioty 305 8 and and CC cord-275413-e2rhioty 305 9 adaptation adaptation NN cord-275413-e2rhioty 305 10 in in IN cord-275413-e2rhioty 305 11 RNA RNA NNP cord-275413-e2rhioty 305 12 viruses virus NNS cord-275413-e2rhioty 305 13 Immune immune VBP cord-275413-e2rhioty 305 14 regulation regulation NN cord-275413-e2rhioty 305 15 during during IN cord-275413-e2rhioty 305 16 pregnancy pregnancy NN cord-275413-e2rhioty 305 17 and and CC cord-275413-e2rhioty 305 18 host host NN cord-275413-e2rhioty 305 19 - - HYPH cord-275413-e2rhioty 305 20 pathogen pathogen NN cord-275413-e2rhioty 305 21 interactions interaction NNS cord-275413-e2rhioty 305 22 in in IN cord-275413-e2rhioty 305 23 infectious infectious JJ cord-275413-e2rhioty 305 24 abortion abortion NN cord-275413-e2rhioty 305 25 Cell cell NN cord-275413-e2rhioty 305 26 cycle cycle NN cord-275413-e2rhioty 305 27 - - HYPH cord-275413-e2rhioty 305 28 dependent dependent JJ cord-275413-e2rhioty 305 29 regulation regulation NN cord-275413-e2rhioty 305 30 of of IN cord-275413-e2rhioty 305 31 CDw75 CDw75 NNS cord-275413-e2rhioty 305 32 ( ( -LRB- cord-275413-e2rhioty 305 33 betagalactoside betagalactoside NNP cord-275413-e2rhioty 305 34 alpha-2,6-sialyltransferase alpha-2,6-sialyltransferase NNP cord-275413-e2rhioty 305 35 ) ) -RRB- cord-275413-e2rhioty 305 36 on on IN cord-275413-e2rhioty 305 37 human human JJ cord-275413-e2rhioty 305 38 B b NN cord-275413-e2rhioty 305 39 lymphocytes lymphocyte NNS cord-275413-e2rhioty 306 1 In in IN cord-275413-e2rhioty 306 2 utero utero NN cord-275413-e2rhioty 306 3 infection infection NN cord-275413-e2rhioty 306 4 by by IN cord-275413-e2rhioty 306 5 porcine porcine JJ cord-275413-e2rhioty 306 6 reproductive reproductive JJ cord-275413-e2rhioty 306 7 and and CC cord-275413-e2rhioty 306 8 respiratory respiratory JJ cord-275413-e2rhioty 306 9 syndrome syndrome NN cord-275413-e2rhioty 306 10 virus virus NN cord-275413-e2rhioty 306 11 is be VBZ cord-275413-e2rhioty 306 12 sufficient sufficient JJ cord-275413-e2rhioty 306 13 to to TO cord-275413-e2rhioty 306 14 increase increase VB cord-275413-e2rhioty 306 15 susceptibility susceptibility NN cord-275413-e2rhioty 306 16 of of IN cord-275413-e2rhioty 306 17 piglets piglet NNS cord-275413-e2rhioty 306 18 to to TO cord-275413-e2rhioty 306 19 challenge challenge VB cord-275413-e2rhioty 306 20 by by IN cord-275413-e2rhioty 306 21 Streptococcus Streptococcus NNP cord-275413-e2rhioty 306 22 suis suis NN cord-275413-e2rhioty 306 23 type type NN cord-275413-e2rhioty 306 24 II II NNP cord-275413-e2rhioty 306 25 Quasispecies Quasispecies NNPS cord-275413-e2rhioty 306 26 in in IN cord-275413-e2rhioty 306 27 viral viral JJ cord-275413-e2rhioty 306 28 persistence persistence NN cord-275413-e2rhioty 306 29 and and CC cord-275413-e2rhioty 306 30 pathogenesis pathogenesis NN cord-275413-e2rhioty 306 31 of of IN cord-275413-e2rhioty 306 32 hepatitis hepatitis NN cord-275413-e2rhioty 306 33 C C NNP cord-275413-e2rhioty 306 34 virus virus NN cord-275413-e2rhioty 306 35 Deficient Deficient NNP cord-275413-e2rhioty 306 36 IL-12(p35 il-12(p35 NN cord-275413-e2rhioty 306 37 ) ) -RRB- cord-275413-e2rhioty 307 1 gene gene NN cord-275413-e2rhioty 307 2 expression expression NN cord-275413-e2rhioty 307 3 by by IN cord-275413-e2rhioty 307 4 dendritic dendritic JJ cord-275413-e2rhioty 307 5 cells cell NNS cord-275413-e2rhioty 307 6 derived derive VBN cord-275413-e2rhioty 307 7 from from IN cord-275413-e2rhioty 307 8 neonatal neonatal JJ cord-275413-e2rhioty 307 9 monocytes monocyte NNS cord-275413-e2rhioty 307 10 Changes change NNS cord-275413-e2rhioty 307 11 in in IN cord-275413-e2rhioty 307 12 intracellular intracellular JJ cord-275413-e2rhioty 307 13 cytokine cytokine NN cord-275413-e2rhioty 307 14 levels level NNS cord-275413-e2rhioty 307 15 in in IN cord-275413-e2rhioty 307 16 newborn newborn JJ cord-275413-e2rhioty 307 17 and and CC cord-275413-e2rhioty 307 18 adult adult NN cord-275413-e2rhioty 307 19 lymphocytes lymphocyte NNS cord-275413-e2rhioty 307 20 induced induce VBN cord-275413-e2rhioty 307 21 by by IN cord-275413-e2rhioty 308 1 HSV-1 HSV-1 NNP cord-275413-e2rhioty 308 2 Cytokine Cytokine NNP cord-275413-e2rhioty 308 3 mRNA mRNA NNP cord-275413-e2rhioty 308 4 profiles profile NNS cord-275413-e2rhioty 308 5 in in IN cord-275413-e2rhioty 308 6 bronchoalveolar bronchoalveolar JJ cord-275413-e2rhioty 308 7 cells cell NNS cord-275413-e2rhioty 308 8 of of IN cord-275413-e2rhioty 308 9 piglets piglet NNS cord-275413-e2rhioty 308 10 experimentally experimentally RB cord-275413-e2rhioty 308 11 infected infect VBN cord-275413-e2rhioty 308 12 in in IN cord-275413-e2rhioty 308 13 utero utero NN cord-275413-e2rhioty 308 14 with with IN cord-275413-e2rhioty 308 15 porcine porcine JJ cord-275413-e2rhioty 308 16 reproductive reproductive JJ cord-275413-e2rhioty 308 17 and and CC cord-275413-e2rhioty 308 18 respiratory respiratory JJ cord-275413-e2rhioty 308 19 syndrome syndrome NN cord-275413-e2rhioty 308 20 virus virus NN cord-275413-e2rhioty 308 21 : : : cord-275413-e2rhioty 308 22 association association NN cord-275413-e2rhioty 308 23 of of IN cord-275413-e2rhioty 308 24 sustained sustained JJ cord-275413-e2rhioty 308 25 expression expression NN cord-275413-e2rhioty 308 26 of of IN cord-275413-e2rhioty 308 27 IFN IFN NNP cord-275413-e2rhioty 308 28 - - HYPH cord-275413-e2rhioty 308 29 gamma gamma NN cord-275413-e2rhioty 308 30 and and CC cord-275413-e2rhioty 309 1 IL-10 IL-10 NNP cord-275413-e2rhioty 309 2 after after IN cord-275413-e2rhioty 309 3 viral viral JJ cord-275413-e2rhioty 309 4 clearance clearance NN cord-275413-e2rhioty 309 5 Gross gross JJ cord-275413-e2rhioty 309 6 and and CC cord-275413-e2rhioty 309 7 microscopic microscopic JJ cord-275413-e2rhioty 309 8 lesions lesion NNS cord-275413-e2rhioty 309 9 in in IN cord-275413-e2rhioty 309 10 porcine porcine JJ cord-275413-e2rhioty 309 11 fetuses fetus NNS cord-275413-e2rhioty 309 12 infected infect VBN cord-275413-e2rhioty 309 13 with with IN cord-275413-e2rhioty 309 14 porcine porcine JJ cord-275413-e2rhioty 309 15 reproductive reproductive JJ cord-275413-e2rhioty 309 16 and and CC cord-275413-e2rhioty 309 17 respiratory respiratory JJ cord-275413-e2rhioty 309 18 syndrome syndrome NN cord-275413-e2rhioty 309 19 virus virus NN cord-275413-e2rhioty 309 20 Interruption interruption NN cord-275413-e2rhioty 309 21 of of IN cord-275413-e2rhioty 309 22 murine murine JJ cord-275413-e2rhioty 309 23 pregnancy pregnancy NN cord-275413-e2rhioty 309 24 by by IN cord-275413-e2rhioty 309 25 activation activation NN cord-275413-e2rhioty 309 26 of of IN cord-275413-e2rhioty 309 27 antigen antigen NN cord-275413-e2rhioty 309 28 - - HYPH cord-275413-e2rhioty 309 29 nonspecific nonspecific JJ cord-275413-e2rhioty 309 30 killer killer NN cord-275413-e2rhioty 309 31 cells cell NNS cord-275413-e2rhioty 309 32 in in IN cord-275413-e2rhioty 309 33 the the DT cord-275413-e2rhioty 309 34 endometrium endometrium NN cord-275413-e2rhioty 309 35 with with IN cord-275413-e2rhioty 309 36 endomethacin endomethacin NNS cord-275413-e2rhioty 309 37 , , , cord-275413-e2rhioty 309 38 high high JJ cord-275413-e2rhioty 309 39 dose dose NN cord-275413-e2rhioty 309 40 IL-2 il-2 NN cord-275413-e2rhioty 309 41 , , , cord-275413-e2rhioty 309 42 or or CC cord-275413-e2rhioty 309 43 a a DT cord-275413-e2rhioty 309 44 combination combination NN cord-275413-e2rhioty 310 1 Neonatal neonatal JJ cord-275413-e2rhioty 310 2 dendritic dendritic JJ cord-275413-e2rhioty 310 3 cells cell NNS cord-275413-e2rhioty 310 4 are be VBP cord-275413-e2rhioty 310 5 intrinsically intrinsically RB cord-275413-e2rhioty 310 6 biased biased JJ cord-275413-e2rhioty 310 7 against against IN cord-275413-e2rhioty 310 8 Th-1 Th-1 NNP cord-275413-e2rhioty 310 9 immune immune JJ cord-275413-e2rhioty 310 10 responses response NNS cord-275413-e2rhioty 311 1 Molecular molecular JJ cord-275413-e2rhioty 311 2 cloning cloning NN cord-275413-e2rhioty 311 3 and and CC cord-275413-e2rhioty 311 4 expression expression NN cord-275413-e2rhioty 311 5 analysis analysis NN cord-275413-e2rhioty 311 6 of of IN cord-275413-e2rhioty 311 7 pig pig NN cord-275413-e2rhioty 311 8 CD79alpha CD79alpha NNP cord-275413-e2rhioty 311 9 Synthesis Synthesis NNP cord-275413-e2rhioty 311 10 of of IN cord-275413-e2rhioty 311 11 T T NNP cord-275413-e2rhioty 311 12 helper helper NN cord-275413-e2rhioty 311 13 2-type 2-type CD cord-275413-e2rhioty 311 14 cytokines cytokine NNS cord-275413-e2rhioty 311 15 at at IN cord-275413-e2rhioty 311 16 the the DT cord-275413-e2rhioty 311 17 maternal maternal JJ cord-275413-e2rhioty 311 18 - - HYPH cord-275413-e2rhioty 311 19 fetal fetal JJ cord-275413-e2rhioty 311 20 interface interface NN cord-275413-e2rhioty 311 21 Cytokine cytokine NN cord-275413-e2rhioty 311 22 receptor receptor NN cord-275413-e2rhioty 311 23 signalling signal VBG cord-275413-e2rhioty 311 24 in in IN cord-275413-e2rhioty 311 25 neonatal neonatal JJ cord-275413-e2rhioty 311 26 macrophages macrophage NNS cord-275413-e2rhioty 311 27 : : : cord-275413-e2rhioty 311 28 defective defective JJ cord-275413-e2rhioty 311 29 STAT-1 STAT-1 NNS cord-275413-e2rhioty 311 30 phosphorylation phosphorylation NN cord-275413-e2rhioty 311 31 in in IN cord-275413-e2rhioty 311 32 response response NN cord-275413-e2rhioty 311 33 to to IN cord-275413-e2rhioty 311 34 stimulation stimulation NN cord-275413-e2rhioty 311 35 with with IN cord-275413-e2rhioty 311 36 IFN IFN NNP cord-275413-e2rhioty 311 37 - - HYPH cord-275413-e2rhioty 311 38 gamma gamma NNP cord-275413-e2rhioty 311 39 Hypomethylation Hypomethylation NNP cord-275413-e2rhioty 311 40 of of IN cord-275413-e2rhioty 311 41 the the DT cord-275413-e2rhioty 311 42 interferon interferon NN cord-275413-e2rhioty 311 43 - - HYPH cord-275413-e2rhioty 311 44 gamma gamma NN cord-275413-e2rhioty 311 45 gene gene NN cord-275413-e2rhioty 311 46 correlates correlate VBZ cord-275413-e2rhioty 311 47 with with IN cord-275413-e2rhioty 311 48 its -PRON- PRP$ cord-275413-e2rhioty 311 49 expression expression NN cord-275413-e2rhioty 311 50 by by IN cord-275413-e2rhioty 311 51 primary primary JJ cord-275413-e2rhioty 311 52 T t NN cord-275413-e2rhioty 311 53 - - HYPH cord-275413-e2rhioty 311 54 lineage lineage NN cord-275413-e2rhioty 311 55 cells cell NNS cord-275413-e2rhioty 311 56 Temporal temporal JJ cord-275413-e2rhioty 311 57 characterization characterization NN cord-275413-e2rhioty 311 58 of of IN cord-275413-e2rhioty 311 59 transplacental transplacental JJ cord-275413-e2rhioty 311 60 infection infection NN cord-275413-e2rhioty 311 61 of of IN cord-275413-e2rhioty 311 62 porcine porcine JJ cord-275413-e2rhioty 311 63 fetuses fetus NNS cord-275413-e2rhioty 311 64 with with IN cord-275413-e2rhioty 311 65 porcine porcine JJ cord-275413-e2rhioty 311 66 reproductive reproductive JJ cord-275413-e2rhioty 311 67 and and CC cord-275413-e2rhioty 311 68 respiratory respiratory JJ cord-275413-e2rhioty 311 69 syndrome syndrome NN cord-275413-e2rhioty 311 70 virus virus NN cord-275413-e2rhioty 311 71 Comparison comparison NN cord-275413-e2rhioty 311 72 among among IN cord-275413-e2rhioty 311 73 strains strain NNS cord-275413-e2rhioty 311 74 of of IN cord-275413-e2rhioty 311 75 porcine porcine JJ cord-275413-e2rhioty 311 76 reproductive reproductive JJ cord-275413-e2rhioty 311 77 and and CC cord-275413-e2rhioty 311 78 respiratory respiratory JJ cord-275413-e2rhioty 311 79 syndrome syndrome NN cord-275413-e2rhioty 311 80 virus virus NN cord-275413-e2rhioty 311 81 for for IN cord-275413-e2rhioty 311 82 their -PRON- PRP$ cord-275413-e2rhioty 311 83 ability ability NN cord-275413-e2rhioty 311 84 to to TO cord-275413-e2rhioty 311 85 cause cause VB cord-275413-e2rhioty 311 86 reproductive reproductive JJ cord-275413-e2rhioty 311 87 failure failure NN cord-275413-e2rhioty 312 1 Interferon interferon NN cord-275413-e2rhioty 312 2 gamma gamma NN cord-275413-e2rhioty 312 3 in in IN cord-275413-e2rhioty 312 4 successful successful JJ cord-275413-e2rhioty 312 5 pregnancies pregnancy NNS cord-275413-e2rhioty 313 1 Porcine porcine JJ cord-275413-e2rhioty 313 2 reproductive reproductive JJ cord-275413-e2rhioty 313 3 and and CC cord-275413-e2rhioty 313 4 respiratory respiratory JJ cord-275413-e2rhioty 313 5 syndrome syndrome NN cord-275413-e2rhioty 313 6 virus virus NN cord-275413-e2rhioty 313 7 comparison comparison NN cord-275413-e2rhioty 313 8 : : : cord-275413-e2rhioty 313 9 divergent divergent JJ cord-275413-e2rhioty 313 10 evolution evolution NN cord-275413-e2rhioty 313 11 on on IN cord-275413-e2rhioty 313 12 two two CD cord-275413-e2rhioty 313 13 continents continent NNS cord-275413-e2rhioty 313 14 Differentiation differentiation NN cord-275413-e2rhioty 313 15 of of IN cord-275413-e2rhioty 313 16 United United NNP cord-275413-e2rhioty 313 17 States States NNP cord-275413-e2rhioty 313 18 and and CC cord-275413-e2rhioty 313 19 European european JJ cord-275413-e2rhioty 313 20 isolates isolate NNS cord-275413-e2rhioty 313 21 of of IN cord-275413-e2rhioty 313 22 porcine porcine JJ cord-275413-e2rhioty 313 23 reproductive reproductive JJ cord-275413-e2rhioty 313 24 and and CC cord-275413-e2rhioty 313 25 respiratory respiratory JJ cord-275413-e2rhioty 313 26 syndrome syndrome NN cord-275413-e2rhioty 313 27 ( ( -LRB- cord-275413-e2rhioty 313 28 PRRS PRRS NNP cord-275413-e2rhioty 313 29 ) ) -RRB- cord-275413-e2rhioty 313 30 virus virus NN cord-275413-e2rhioty 313 31 using use VBG cord-275413-e2rhioty 313 32 monoclonal monoclonal JJ cord-275413-e2rhioty 313 33 antibodies antibody NNS cord-275413-e2rhioty 313 34 Lactate lactate NN cord-275413-e2rhioty 313 35 dehydrogenase dehydrogenase NN cord-275413-e2rhioty 313 36 - - HYPH cord-275413-e2rhioty 313 37 elevating elevate VBG cord-275413-e2rhioty 313 38 virus virus NN cord-275413-e2rhioty 313 39 and and CC cord-275413-e2rhioty 313 40 related relate VBN cord-275413-e2rhioty 313 41 viruses virus NNS cord-275413-e2rhioty 313 42 Transplacental Transplacental NNP cord-275413-e2rhioty 313 43 priming priming NN cord-275413-e2rhioty 313 44 of of IN cord-275413-e2rhioty 313 45 the the DT cord-275413-e2rhioty 313 46 human human JJ cord-275413-e2rhioty 313 47 immune immune JJ cord-275413-e2rhioty 313 48 system system NN cord-275413-e2rhioty 313 49 to to IN cord-275413-e2rhioty 313 50 environmental environmental JJ cord-275413-e2rhioty 313 51 allergens allergen NNS cord-275413-e2rhioty 313 52 : : : cord-275413-e2rhioty 313 53 universal universal JJ cord-275413-e2rhioty 313 54 skewing skewing NN cord-275413-e2rhioty 313 55 of of IN cord-275413-e2rhioty 313 56 initial initial JJ cord-275413-e2rhioty 313 57 T T NNP cord-275413-e2rhioty 313 58 cell cell NN cord-275413-e2rhioty 313 59 responses response NNS cord-275413-e2rhioty 313 60 toward toward IN cord-275413-e2rhioty 313 61 the the DT cord-275413-e2rhioty 313 62 Th2 th2 CD cord-275413-e2rhioty 313 63 cytokine cytokine NN cord-275413-e2rhioty 313 64 profile profile NN cord-275413-e2rhioty 314 1 Pregnancy pregnancy NN cord-275413-e2rhioty 314 2 : : : cord-275413-e2rhioty 314 3 success success NN cord-275413-e2rhioty 314 4 and and CC cord-275413-e2rhioty 314 5 failure failure NN cord-275413-e2rhioty 314 6 within within IN cord-275413-e2rhioty 314 7 the the DT cord-275413-e2rhioty 314 8 Th1 th1 CD cord-275413-e2rhioty 314 9 / / SYM cord-275413-e2rhioty 314 10 Th2 th2 CD cord-275413-e2rhioty 314 11 / / SYM cord-275413-e2rhioty 314 12 Th3 th3 CD cord-275413-e2rhioty 314 13 paradigm paradigm NN cord-275413-e2rhioty 314 14 Interleukin Interleukin NNP cord-275413-e2rhioty 314 15 10 10 CD cord-275413-e2rhioty 314 16 , , , cord-275413-e2rhioty 314 17 produced produce VBN cord-275413-e2rhioty 314 18 in in IN cord-275413-e2rhioty 314 19 abundance abundance NN cord-275413-e2rhioty 314 20 by by IN cord-275413-e2rhioty 314 21 human human JJ cord-275413-e2rhioty 314 22 newborn newborn NN cord-275413-e2rhioty 314 23 T T NNP cord-275413-e2rhioty 314 24 cells cell NNS cord-275413-e2rhioty 314 25 , , , cord-275413-e2rhioty 314 26 may may MD cord-275413-e2rhioty 314 27 be be VB cord-275413-e2rhioty 314 28 the the DT cord-275413-e2rhioty 314 29 regulator regulator NN cord-275413-e2rhioty 314 30 of of IN cord-275413-e2rhioty 314 31 increased increase VBN cord-275413-e2rhioty 314 32 tolerance tolerance NN cord-275413-e2rhioty 314 33 associated associate VBN cord-275413-e2rhioty 314 34 with with IN cord-275413-e2rhioty 314 35 cord cord NN cord-275413-e2rhioty 314 36 blood blood NN cord-275413-e2rhioty 314 37 stem stem NN cord-275413-e2rhioty 314 38 cell cell NN cord-275413-e2rhioty 314 39 transplantation transplantation NN cord-275413-e2rhioty 314 40 Quantitation Quantitation NNP cord-275413-e2rhioty 314 41 of of IN cord-275413-e2rhioty 314 42 porcine porcine JJ cord-275413-e2rhioty 314 43 cytokine cytokine NN cord-275413-e2rhioty 314 44 and and CC cord-275413-e2rhioty 314 45 beta beta JJ cord-275413-e2rhioty 314 46 2-microglobulin 2-microglobulin CD cord-275413-e2rhioty 314 47 mRNA mrna NN cord-275413-e2rhioty 314 48 expression expression NN cord-275413-e2rhioty 314 49 by by IN cord-275413-e2rhioty 314 50 reverse reverse JJ cord-275413-e2rhioty 314 51 transcription transcription NN cord-275413-e2rhioty 314 52 polymerase polymerase NN cord-275413-e2rhioty 314 53 chain chain NN cord-275413-e2rhioty 314 54 reaction reaction NN cord-275413-e2rhioty 314 55 Porcine porcine JJ cord-275413-e2rhioty 314 56 reproductive reproductive JJ cord-275413-e2rhioty 314 57 and and CC cord-275413-e2rhioty 314 58 respiratory respiratory JJ cord-275413-e2rhioty 314 59 syndrome syndrome NN cord-275413-e2rhioty 314 60 Lymph lymph NN cord-275413-e2rhioty 314 61 node node NN cord-275413-e2rhioty 314 62 lesions lesion NNS cord-275413-e2rhioty 314 63 in in IN cord-275413-e2rhioty 314 64 neonatal neonatal JJ cord-275413-e2rhioty 314 65 pigs pig NNS cord-275413-e2rhioty 314 66 congenitally congenitally RB cord-275413-e2rhioty 314 67 exposed expose VBN cord-275413-e2rhioty 314 68 to to IN cord-275413-e2rhioty 314 69 porcine porcine JJ cord-275413-e2rhioty 314 70 reproductive reproductive JJ cord-275413-e2rhioty 314 71 and and CC cord-275413-e2rhioty 314 72 respiratory respiratory JJ cord-275413-e2rhioty 314 73 syndrome syndrome NN cord-275413-e2rhioty 314 74 virus virus NN cord-275413-e2rhioty 314 75 Porcine porcine JJ cord-275413-e2rhioty 314 76 reproductive reproductive JJ cord-275413-e2rhioty 314 77 and and CC cord-275413-e2rhioty 314 78 respiratory respiratory JJ cord-275413-e2rhioty 314 79 syndrome syndrome NN cord-275413-e2rhioty 314 80 virus virus NN cord-275413-e2rhioty 314 81 infection infection NN cord-275413-e2rhioty 314 82 in in IN cord-275413-e2rhioty 314 83 neonatal neonatal JJ cord-275413-e2rhioty 314 84 pigs pig NNS cord-275413-e2rhioty 314 85 characterised characterise VBN cord-275413-e2rhioty 314 86 by by IN cord-275413-e2rhioty 314 87 marked mark VBN cord-275413-e2rhioty 314 88 neurovirulence neurovirulence NN cord-275413-e2rhioty 315 1 The the DT cord-275413-e2rhioty 315 2 evolution evolution NN cord-275413-e2rhioty 315 3 of of IN cord-275413-e2rhioty 315 4 porcine porcine JJ cord-275413-e2rhioty 315 5 reproductive reproductive JJ cord-275413-e2rhioty 315 6 and and CC cord-275413-e2rhioty 315 7 respiratory respiratory JJ cord-275413-e2rhioty 315 8 syndrome syndrome NN cord-275413-e2rhioty 315 9 virus virus NN cord-275413-e2rhioty 315 10 : : : cord-275413-e2rhioty 315 11 quasispecies quasispecie NNS cord-275413-e2rhioty 315 12 and and CC cord-275413-e2rhioty 315 13 emergence emergence NN cord-275413-e2rhioty 315 14 of of IN cord-275413-e2rhioty 315 15 a a DT cord-275413-e2rhioty 315 16 virus virus NN cord-275413-e2rhioty 315 17 subpopulation subpopulation NN cord-275413-e2rhioty 315 18 during during IN cord-275413-e2rhioty 315 19 infection infection NN cord-275413-e2rhioty 315 20 of of IN cord-275413-e2rhioty 315 21 pigs pig NNS cord-275413-e2rhioty 315 22 with with IN cord-275413-e2rhioty 315 23 VR-2332 vr-2332 NN cord-275413-e2rhioty 315 24 Lymphoid lymphoid JJ cord-275413-e2rhioty 315 25 tissue tissue NN cord-275413-e2rhioty 315 26 tropism tropism NN cord-275413-e2rhioty 315 27 of of IN cord-275413-e2rhioty 315 28 porcine porcine JJ cord-275413-e2rhioty 315 29 reproductive reproductive JJ cord-275413-e2rhioty 315 30 and and CC cord-275413-e2rhioty 315 31 respiratory respiratory JJ cord-275413-e2rhioty 315 32 syndrome syndrome NN cord-275413-e2rhioty 315 33 virus virus NN cord-275413-e2rhioty 315 34 replication replication NN cord-275413-e2rhioty 315 35 during during IN cord-275413-e2rhioty 315 36 persistent persistent JJ cord-275413-e2rhioty 315 37 infection infection NN cord-275413-e2rhioty 315 38 of of IN cord-275413-e2rhioty 315 39 pigs pig NNS cord-275413-e2rhioty 315 40 originally originally RB cord-275413-e2rhioty 315 41 exposed expose VBN cord-275413-e2rhioty 315 42 to to IN cord-275413-e2rhioty 315 43 virus virus NN cord-275413-e2rhioty 315 44 in in IN cord-275413-e2rhioty 315 45 utero utero NN cord-275413-e2rhioty 315 46 Inhibition inhibition NN cord-275413-e2rhioty 315 47 of of IN cord-275413-e2rhioty 315 48 porcine porcine JJ cord-275413-e2rhioty 315 49 reproductive reproductive JJ cord-275413-e2rhioty 315 50 and and CC cord-275413-e2rhioty 315 51 respiratory respiratory JJ cord-275413-e2rhioty 315 52 syndrome syndrome NN cord-275413-e2rhioty 315 53 virus virus NN cord-275413-e2rhioty 315 54 by by IN cord-275413-e2rhioty 315 55 interferon interferon NN cord-275413-e2rhioty 315 56 - - HYPH cord-275413-e2rhioty 315 57 gamma gamma NN cord-275413-e2rhioty 315 58 and and CC cord-275413-e2rhioty 315 59 recovery recovery NN cord-275413-e2rhioty 315 60 of of IN cord-275413-e2rhioty 315 61 virus virus NN cord-275413-e2rhioty 315 62 replication replication NN cord-275413-e2rhioty 315 63 with with IN cord-275413-e2rhioty 315 64 2 2 CD cord-275413-e2rhioty 315 65 aminopurine aminopurine JJ cord-275413-e2rhioty 315 66 Maternal maternal JJ cord-275413-e2rhioty 315 67 influenza influenza NN cord-275413-e2rhioty 315 68 infection infection NN cord-275413-e2rhioty 315 69 causes cause VBZ cord-275413-e2rhioty 315 70 marked mark VBN cord-275413-e2rhioty 315 71 behavioral behavioral JJ cord-275413-e2rhioty 315 72 and and CC cord-275413-e2rhioty 315 73 pharmacological pharmacological JJ cord-275413-e2rhioty 315 74 changes change NNS cord-275413-e2rhioty 315 75 in in IN cord-275413-e2rhioty 315 76 the the DT cord-275413-e2rhioty 315 77 offspring offspring NN cord-275413-e2rhioty 315 78 The the DT cord-275413-e2rhioty 315 79 molecular molecular JJ cord-275413-e2rhioty 315 80 biology biology NN cord-275413-e2rhioty 315 81 of of IN cord-275413-e2rhioty 315 82 arteriviruses arteriviruse NNS cord-275413-e2rhioty 315 83 Uterine uterine NN cord-275413-e2rhioty 315 84 and and CC cord-275413-e2rhioty 315 85 placental placental JJ cord-275413-e2rhioty 315 86 alterations alteration NNS cord-275413-e2rhioty 315 87 in in IN cord-275413-e2rhioty 315 88 pregnant pregnant JJ cord-275413-e2rhioty 315 89 sows sow NNS cord-275413-e2rhioty 315 90 associated associate VBN cord-275413-e2rhioty 315 91 with with IN cord-275413-e2rhioty 315 92 the the DT cord-275413-e2rhioty 315 93 porcine porcine JJ cord-275413-e2rhioty 315 94 epidemic epidemic NN cord-275413-e2rhioty 315 95 abortion abortion NN cord-275413-e2rhioty 315 96 and and CC cord-275413-e2rhioty 315 97 respiratory respiratory JJ cord-275413-e2rhioty 315 98 syndrome syndrome NN cord-275413-e2rhioty 315 99 ( ( -LRB- cord-275413-e2rhioty 315 100 PEARS PEARS NNP cord-275413-e2rhioty 315 101 ) ) -RRB- cord-275413-e2rhioty 316 1 Immunohistochemical immunohistochemical JJ cord-275413-e2rhioty 316 2 staining staining NN cord-275413-e2rhioty 316 3 of of IN cord-275413-e2rhioty 316 4 IFN IFN NNP cord-275413-e2rhioty 316 5 - - HYPH cord-275413-e2rhioty 316 6 gamma gamma NN cord-275413-e2rhioty 316 7 positive positive JJ cord-275413-e2rhioty 316 8 cells cell NNS cord-275413-e2rhioty 316 9 in in IN cord-275413-e2rhioty 316 10 porcine porcine JJ cord-275413-e2rhioty 316 11 reproductive reproductive JJ cord-275413-e2rhioty 316 12 and and CC cord-275413-e2rhioty 316 13 respiratory respiratory JJ cord-275413-e2rhioty 316 14 syndrome syndrome NN cord-275413-e2rhioty 316 15 virus virus NN cord-275413-e2rhioty 316 16 - - HYPH cord-275413-e2rhioty 316 17 infected infect VBN cord-275413-e2rhioty 316 18 lung lung NN cord-275413-e2rhioty 316 19 Immunity immunity NN cord-275413-e2rhioty 316 20 in in IN cord-275413-e2rhioty 316 21 the the DT cord-275413-e2rhioty 316 22 fetus fetus NN cord-275413-e2rhioty 316 23 and and CC cord-275413-e2rhioty 316 24 newborn newborn JJ cord-275413-e2rhioty 316 25 . . . cord-275413-e2rhioty 317 1 In in IN cord-275413-e2rhioty 317 2 : : : cord-275413-e2rhioty 317 3 Veterinary Veterinary NNP cord-275413-e2rhioty 317 4 Immunology Immunology NNP cord-275413-e2rhioty 317 5 : : : cord-275413-e2rhioty 317 6 An an DT cord-275413-e2rhioty 317 7 Introduction introduction NN cord-275413-e2rhioty 317 8 , , , cord-275413-e2rhioty 317 9 Chapter chapter NN cord-275413-e2rhioty 317 10 19 19 CD cord-275413-e2rhioty 317 11 Quantitative quantitative JJ cord-275413-e2rhioty 317 12 deep deep JJ cord-275413-e2rhioty 317 13 sequencing sequencing NN cord-275413-e2rhioty 317 14 reveals reveal VBZ cord-275413-e2rhioty 317 15 dynamic dynamic JJ cord-275413-e2rhioty 317 16 HIV-1 HIV-1 NNP cord-275413-e2rhioty 317 17 escape escape NN cord-275413-e2rhioty 317 18 and and CC cord-275413-e2rhioty 317 19 large large JJ cord-275413-e2rhioty 317 20 population population NN cord-275413-e2rhioty 317 21 shifts shift NNS cord-275413-e2rhioty 317 22 during during IN cord-275413-e2rhioty 317 23 CCR5 CCR5 NNP cord-275413-e2rhioty 317 24 antagonist antagonist NN cord-275413-e2rhioty 317 25 therapy therapy NN cord-275413-e2rhioty 317 26 in in IN cord-275413-e2rhioty 317 27 vivo vivo NNP cord-275413-e2rhioty 317 28 Interferongamma Interferongamma NNP cord-275413-e2rhioty 317 29 - - HYPH cord-275413-e2rhioty 317 30 induced induce VBN cord-275413-e2rhioty 317 31 changes change NNS cord-275413-e2rhioty 317 32 in in IN cord-275413-e2rhioty 317 33 synaptic synaptic JJ cord-275413-e2rhioty 317 34 activity activity NN cord-275413-e2rhioty 317 35 and and CC cord-275413-e2rhioty 317 36 AMPA AMPA NNP cord-275413-e2rhioty 317 37 receptor receptor NN cord-275413-e2rhioty 317 38 clustering clustering NN cord-275413-e2rhioty 317 39 in in IN cord-275413-e2rhioty 317 40 hippocampal hippocampal JJ cord-275413-e2rhioty 317 41 cultures culture NNS cord-275413-e2rhioty 317 42 Differential differential JJ cord-275413-e2rhioty 317 43 patterns pattern NNS cord-275413-e2rhioty 317 44 of of IN cord-275413-e2rhioty 317 45 methylation methylation NN cord-275413-e2rhioty 317 46 of of IN cord-275413-e2rhioty 317 47 the the DT cord-275413-e2rhioty 317 48 IFN IFN NNP cord-275413-e2rhioty 317 49 - - HYPH cord-275413-e2rhioty 317 50 gamma gamma NN cord-275413-e2rhioty 317 51 promoter promoter NN cord-275413-e2rhioty 317 52 at at IN cord-275413-e2rhioty 317 53 CpG CpG NNP cord-275413-e2rhioty 317 54 and and CC cord-275413-e2rhioty 317 55 non non JJ cord-275413-e2rhioty 317 56 - - JJ cord-275413-e2rhioty 317 57 CpG cpg JJ cord-275413-e2rhioty 317 58 sites site NNS cord-275413-e2rhioty 317 59 underlie underlie VBP cord-275413-e2rhioty 317 60 differences difference NNS cord-275413-e2rhioty 317 61 in in IN cord-275413-e2rhioty 317 62 IFN IFN NNP cord-275413-e2rhioty 317 63 - - HYPH cord-275413-e2rhioty 317 64 gamma gamma NN cord-275413-e2rhioty 317 65 gene gene NN cord-275413-e2rhioty 317 66 expression expression NN cord-275413-e2rhioty 317 67 between between IN cord-275413-e2rhioty 317 68 human human JJ cord-275413-e2rhioty 317 69 neonatal neonatal NN cord-275413-e2rhioty 317 70 and and CC cord-275413-e2rhioty 317 71 adult adult NN cord-275413-e2rhioty 317 72 CD45RO CD45RO NNP cord-275413-e2rhioty 317 73 - - HYPH cord-275413-e2rhioty 317 74 T t NN cord-275413-e2rhioty 317 75 cells cell NNS cord-275413-e2rhioty 317 76 Primary primary JJ cord-275413-e2rhioty 317 77 immune immune JJ cord-275413-e2rhioty 317 78 responses response NNS cord-275413-e2rhioty 317 79 by by IN cord-275413-e2rhioty 317 80 cord cord NN cord-275413-e2rhioty 317 81 blood blood NN cord-275413-e2rhioty 317 82 CD4(+ CD4(+ NNS cord-275413-e2rhioty 317 83 ) ) -RRB- cord-275413-e2rhioty 318 1 T T NNP cord-275413-e2rhioty 318 2 cells cell NNS cord-275413-e2rhioty 318 3 and and CC cord-275413-e2rhioty 318 4 NK NK NNP cord-275413-e2rhioty 318 5 cells cell NNS cord-275413-e2rhioty 318 6 inhibit inhibit VBP cord-275413-e2rhioty 318 7 Epstein Epstein NNP cord-275413-e2rhioty 318 8 - - HYPH cord-275413-e2rhioty 318 9 Barr Barr NNP cord-275413-e2rhioty 318 10 virus virus NN cord-275413-e2rhioty 318 11 B b NN cord-275413-e2rhioty 318 12 - - HYPH cord-275413-e2rhioty 318 13 cell cell NN cord-275413-e2rhioty 318 14 transformation transformation NN cord-275413-e2rhioty 318 15 in in IN cord-275413-e2rhioty 318 16 vitro vitro FW cord-275413-e2rhioty 318 17 This this DT cord-275413-e2rhioty 318 18 work work NN cord-275413-e2rhioty 318 19 was be VBD cord-275413-e2rhioty 318 20 partially partially RB cord-275413-e2rhioty 318 21 supported support VBN cord-275413-e2rhioty 318 22 by by IN cord-275413-e2rhioty 318 23 the the DT cord-275413-e2rhioty 318 24 USDA USDA NNP cord-275413-e2rhioty 318 25 National National NNP cord-275413-e2rhioty 318 26 Research Research NNP cord-275413-e2rhioty 318 27 Initiative Initiative NNP cord-275413-e2rhioty 318 28 for for IN cord-275413-e2rhioty 318 29 Competitive Competitive NNP cord-275413-e2rhioty 318 30 Grants Grants NNPS cord-275413-e2rhioty 318 31 Program Program NNP cord-275413-e2rhioty 318 32 Grants Grants NNPS cord-275413-e2rhioty 318 33 # # $ cord-275413-e2rhioty 318 34 97 97 CD cord-275413-e2rhioty 318 35 - - HYPH cord-275413-e2rhioty 318 36 35204 35204 CD cord-275413-e2rhioty 318 37 - - HYPH cord-275413-e2rhioty 318 38 5071 5071 CD cord-275413-e2rhioty 318 39 . . .