id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-010369-x9z8dg6a	Saito, Kyoko	Comparative characterization of flavivirus production in two cell lines: Human hepatoma-derived Huh7.5.1-8 and African green monkey kidney-derived Vero	2020-04-24		.txt	text/plain	5030	271	61	Quantification of cellular and extracellular viral RNA revealed that high JEV production in Huh7.5.1–8 cells can be attributed to rapid viral replication kinetics and efficient virus release early in infection. Huh7.5.1-8 and Vero cells under high and low confluency were infected with JEV at MOI 0.1, and then the virus titer in culture supernatant was monitored from 1 to 4 d pi (Fig 2) . The culture supernatant of Huh7.5.1-8 cells exhibited a higher relative virus titer than that of Vero cells, particularly during the early infection times. These results suggested that the Huh7.5.1-8 cell line, compared with the Vero cell line, has a higher virus productivity and susceptibility to virus-induced cell death upon flavivirus infection. First, Huh7.5.1-8 cells produced higher amounts of infectious JEV and YFV than Vero cells early in infection (Figs 2 and 8A-8D ).	./cache/cord-010369-x9z8dg6a.txt	./txt/cord-010369-x9z8dg6a.txt