id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-254916-y1rw9q11	Ogando, Natacha S.	SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology	2020-06-22		.txt	text/plain	8571	423	50	The overall level of amino acid sequence identity of viral proteins ranges from about 65 % in the least conserved parts of the S protein to about 95 % in the most conserved replicative enzyme domains, prompting the coronavirus study group of the International Committee on the Taxonomy of Viruses to classify the new agent within the species Severe acute respiratory syndrome-related coronavirus, which also includes the 2003 SARS-CoV [1] . In this report, we describe a comparative study of the basic replication features of SARS-CoV and SARS-CoV-2 in Vero E6 cells, including growth kinetics, virus titres, plaque phenotype and an analysis of intracellular viral RNA and protein synthesis. One of them is the rapid evolution -during virus passaging in Vero cells -of a specific region of the SARS-CoV-2 S protein that contains the so-called furin-like cleavage site.	./cache/cord-254916-y1rw9q11.txt	./txt/cord-254916-y1rw9q11.txt