id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267446-rpv19oy6	Park, Jung-Eun	Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion	2011-06-12		.txt	text/plain	4075	186	47	The addition of trypsin was shown to induce fusion of the infected Vero cells, resulting in the formation of multiple syncytia, and produced a significant increase in virus titer after several passages. For example, infection by severe acute respiratory syndrome coronavirus (SARS-CoV) and murine hepatitis virus strain 2 (MHV-2) requires proteolytic cleavage in their target cells, which is mediated by trypsin-like proteases [24, 29, 32] . To investigate the effects of proteolytic cleavage of the surface protein of Vero cells and free virions by trypsin, Vero cells or KPEDV-9 were pre-treated with trypsin prior to infection. These results are consistent with the suggestion that trypsin is not absolutely essential for Vero-cell-adapted PEDV infection, as reported for other group 1 coronaviruses, but the titer increases during infection with trypsin-treated virus.	./cache/cord-267446-rpv19oy6.txt	./txt/cord-267446-rpv19oy6.txt