id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-273745-mwjh5se7	Meng, Fandan	A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry	2014-03-25		.txt	text/plain	5514	291	58	Three phage-displayed peptides designated H, S and F that recognize porcine aminopeptidase N (pAPN), the cellular receptor of porcine transmissible gastroenteritis virus (TGEV) were able to inhibit cell infection by TGEV. When Vero cells were pre-treated with peptide (cell pretreatment assay) prior to virus infection (Fig. 1B) , little changes in virus titers were observed between the control and peptide treatment groups; some small effects were observed with the rabbit anti-PEDV neutralizing antibodies. Finally, in the virus pretreatment assays where PEDV was incubated with peptides prior to cell infection (Fig. 1C) , the results indicated that both peptides H and S inhibited PEDV infectivity where EC 50 values were approximately 1 μg/ml and 62.5 μg/ml, respectively. Results clearly show that peptide H had no demonstrable effects on TGEV or PrV even at very high peptide concentrations (1 mM/ml) (Fig. 3) suggesting that a non-specific reactivity with virus envelopes is unlikely to be the cause for attenuating PEDV infectivity.	./cache/cord-273745-mwjh5se7.txt	./txt/cord-273745-mwjh5se7.txt