id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274110-nyyunoha	Orlinger, Klaus K.	An inactivated West Nile Virus vaccine derived from a chemically synthesized cDNA system	2010-04-26		.txt	text/plain	5113	271	50	A cDNA comprising the complete genome of West Nile Virus (WNV) was generated by chemical synthesis using published sequence data, independent of any preformed viral components. The synthetic WNV, produced by transfection of in vitro transcribed RNA into cell culture, exhibited undistinguishable biological properties compared to the corresponding animal-derived wild-type virus. Taking advantage of the rapid progression of gene synthesis technology (for review [24] ), we intended to adopt such a synthetic approach to produce a flavivirus cDNA system for the generation of a synthetic WNV seed virus for use in vaccine development. The production and characterization of the resulting West Nile Virus, which fully matched the sequence of the in silico designed viral genome, confirms the feasibility and accuracy of the synthetic flavivirus reverse genetic system. Cover slips with fixed cells were dried, rehydrated with phosphate-buffered saline and treated with a polyclonal mouse anti-WNV serum (1:50 dilution) obtained after immunization of mice with a formalin-inactivated whole virus vaccine preparation.	./cache/cord-274110-nyyunoha.txt	./txt/cord-274110-nyyunoha.txt