id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316908-8ti75mru	Wei, Xiaona	PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway	2020-02-10		.txt	text/plain	10503	570	59	Given that the differences in gene sequences and pathogenicity between classical and mutant strains of PEDV may lead to diverse invasion mechanisms, this study focused on the cellular entry pathways and cellular transport of the PEDV GI and GII subtype strains in Vero cells and IPEC-J2 cells. To clarify the specific endocytic pathways, systematic research methods were used and showed that PEDV enters cells via the clathrinand caveolae-mediated endocytosis pathways, in which dynamin II, clathrin heavy chain, Eps15, cholesterol, and caveolin-1 were indispensably involved. Our results showed that two the subtypes of PEDV utilized clathrin-, caveolae-, and lipid raft-mediated endocytosis to enter the Vero and IPEC-J2 cells, but the utilization efficiency of each endocytic pathway varied depending on the different genotypes and types of cells. We demonstrated that PEDV GI subtype GDS09 and GII subtype GDS01 strains could enter Vero and IPEC-J2 cells via the clathrin-, caveolae-, and lipid raft-mediated endocytosis pathways.	./cache/cord-316908-8ti75mru.txt	./txt/cord-316908-8ti75mru.txt