id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349689-njb6619x	Khan, Mohsin	Assessment of in vitro prophylactic and therapeutic efficacy of chloroquine against chikungunya virus in vero cells	2010-03-24		.txt	text/plain	3937	201	51	The goal of the current study was to evaluate the doseand time-dependent effects of chloroquine on CHIKV replication, and to elucidate the mechanism of viral inhibition in Vero cells. Anti-viral activity was assessed by performing cell viability assays on cells that had been infected with CHIKV in the presence of various concentrations of ammonium chloride, ribavirin, or chloroquine. The mechanism of inhibition of CHIKV activity by chloroquine was assessed by comparing the effects of chloroquine to those of a known lysomotropic agent (ammonium chloride) that interferes with early stages of infection, and a standard anti-viral compound (ribavirin) that inhibits virus replication during late stages of infection. To determine the anti-CHIKV activity of chloroquine, we analyzed virus yield in Vero cells treated with drug as compared to untreated infected control cells. We demonstrated that chloroquine is an effective anti-viral agent against CHIKV infection in Vero cells in culture, thus, demonstrating the in vitro prophylactic and therapeutic potential of chloroquine in inhibiting CHIVK infection.	./cache/cord-349689-njb6619x.txt	./txt/cord-349689-njb6619x.txt