id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-355440-20yq6zj0	Klingström, Jonas	Nitric oxide and peroxynitrite have different antiviral effects against hantavirus replication and free mature virions	2006-09-06		.txt	text/plain	4824	222	51	In this study, we compared the effects of NO and peroxynitrite on hantavirus replication and free mature virions in vitro, and of inducible nitric oxide synthase (iNOS) in hantavirus‐infected suckling mice. In the present study, we investigated the effect of NO and peroxynitrite on hantavirus replication in Vero E6 cells and on free mature virions by using S-nitroso-Nacetylpenicillamine (SNAP; an NO-donor), 3-morpholinosydnonimine hydrochloride (SIN-1; a peroxynitrite donor), and by stimulating endogenous NO production by iNOS with cytokines. From cells treated with 50 lM SNAP, 85% less viable virus was obtained, whereas lower concentrations of SNAP had no detectable effect on the virus replication, as compared to NAP-treated or medium controls ( Fig. 2A , and data not shown). However, the finding that iNOS -/suckling mice had higher levels of replicating virus than controls is in line with the finding that cytokine-stimulated NO production inhibited hantavirus replication in Vero E6 cells.	./cache/cord-355440-20yq6zj0.txt	./txt/cord-355440-20yq6zj0.txt