id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000777-7cty5s6o	Merten, O.-W.	Virus contaminations of cell cultures – A biotechnological view	2002-01-01		.txt	text/plain	14936	650	48	These may include the use of production media devoid of animal derived substances, validation of viral clearance in downstream processing or analytics for detecting adventitious viruses in cell culture and final biological product. However, the best means to increase the biological safety of the produced viral vaccines is the use of diploid or continuous cell lines, because it can be determined that such cells are free of animal derived viruses: This can be achieved by establishing master (seed stock) and working (distribution and user stocks) cell banks which have been rigorously tested and validated for the absence of microbial as well as viral contaminants (see chapter by Freshney and the section on 'Testing-virus screening in cell banks' of this article). As animal derived substances such as serum can be contaminated by adventitious viruses, γ irradition is, after routine quality control for virus detection, the best method to increase the safety of using serum in the production of animal cell culture derived biologicals.	./cache/cord-000777-7cty5s6o.txt	./txt/cord-000777-7cty5s6o.txt