id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001985-iwfidoer	Urayama, Syun-ichi	FLDS: A Comprehensive dsRNA Sequencing Method for Intracellular RNA Virus Surveillance	2016-02-13		.txt	text/plain	5007	281	50	This method revealed a previously unidentified viral RNA diversity of more than 20 complete RNA viral genomes including dsRNA and ssRNA viruses associated with an environmental diatom colony. We herein established a novel strategy to obtain full-length RNA virus sequences with extremely high efficiency by applying a short dsRNA full-length cloning method (8) for physically fragmented dsRNAs. The improved method, named FLDS (fragmented and loop primer ligated dsRNA sequencing), was applied to a diatom colony in a tide pool and revealed previously unidentified RNA viruses. These results indicated that FLDS effectively enriched dsRNA reads, thereby allowing the retrieval of complete genome sequences including terminal regions without the requirement for the additional rapid amplification of cDNA ends (RACE). A phylogenetic analysis of RdRp in DCASSRV-2 suggested that the RNA virus was classified into the genus Mitovirus, which has a non-segmented ssRNA genome, infects the mitochondria of fungi, and lacks viral particles (Fig. S4E) .	./cache/cord-001985-iwfidoer.txt	./txt/cord-001985-iwfidoer.txt