id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002274-6rddtogo	James, Joe	Influenza A virus PB1-F2 protein prolongs viral shedding in chickens lengthening the transmission window	2016-10-13		.txt	text/plain	6599	311	52	Here we report that the presence of a full-length PB1-F2 protein, from a low pathogenicity H9N2 avian influenza virus, prolongs infectious virus shedding from directly inoculated chickens, thereby enhancing transmission of the virus by lengthening the transmission window to contact birds. Spillover of these viruses into domesticated poultry populations has resulted in the evolution of highly pathogenic avian influenza (HPAI) subtypes such as the Asian-lineage H5N1 which has been circulating continuously in birds since 2003 and has caused upwards of 700 human infections . To ascertain if the presence of a full-length PB1-F2 protein effected in vivo virus replication in chickens, we determined the level of infectious virus shed from both the buccal and cloacal cavities of birds inoculated with both high and low doses for 10 days following infection. We assessed viral infectivity, pathogenicity and transmissibility between chickens of isogenic viruses differing only by the presence of a full-length PB1-F2 protein.	./cache/cord-002274-6rddtogo.txt	./txt/cord-002274-6rddtogo.txt