id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-002407-25cawzi0	Nogales, Aitor	Reverse Genetics Approaches for the Development of Influenza Vaccines	2016-12-22		.txt	text/plain	11127	598	41	To date, three types of influenza virus vaccines are approved by the Food and Drug Administration (FDA) for human use: recombinant viral HA, inactivated virus and live-attenuated viruses [65] [66] [67] [68] [69] . Plasmid-based reverse genetics for influenza virus allows for the simultaneous expression of the viral components involved in viral genome replication and gene transcription (PB2, PB1, PA and NP) and the eight negative-stranded vRNAs in transfected susceptible cells, which together generate de novo, recombinant IAVs or IBVs (Figure 3 ) [48] . Plasmid-based reverse genetic technologies have allowed the engineering of recombinant influenza viruses that contain single or multiple mutations in the viral genome, which can be potentially implemented as novel or improved vaccine approaches. Importantly, influenza viruses generated by codon deoptimization showed similar viral replication kinetics to WT virus in MDCK cells, which is important for their effective use for vaccine production.	./cache/cord-002407-25cawzi0.txt	./txt/cord-002407-25cawzi0.txt