id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-003880-uuuzfyjm	Shen, Meng-xin	Antiviral Properties of R. tanguticum Nanoparticles on Herpes Simplex Virus Type I In Vitro and In Vivo	2019-09-04		.txt	text/plain	6674	346	49	We first conducted plaque reduction assays using HEp-2 cells to test the capacity of these nanoparticles to inactivate the HSV-1 virions and block the viral attachment and entry into cells, following the evaluation of inhibitory effect on viral replication using real-time quantitative PCR, Western blot, and immunofluorescence methods. tanguticum nanoparticles were added to HEp-2 cell monolayers at indicated time periods after HSV-1 infection (MOI = 0.01). tanguticum nanoparticles could suppress HSV-1 viral plaque formation in a dose-dependent manner ( Figure 3B ) and with the increase of drug concentrations, a noted increased antiviral activity was observed. Our previous study showed that the ethanol extracts of Rheum tanguticum, emodin inhibit the herpes simplex virus in vitro and in vivo, which the most effective dose orally administered to mice was 6.7 g/kg/day (Xiong et al., 2011) .	./cache/cord-003880-uuuzfyjm.txt	./txt/cord-003880-uuuzfyjm.txt