id author title date pages extension mime words sentences flesch summary cache txt cord-004775-foaf3vyl Weiss, Marianne The proposed family toroviridae: Agents of enteric infections 1987 .txt text/plain 3946 231 56 A morphologically similar virus (Lyon 4 virus) detected in cattle in Lyon, France (6, 7) , was shown later to possess an antigenic relatedness to the Berne (BEV) and Breda (BRV) viruses. In thin sections through BEV infected cells (horse kidney, embryonic mule skin, equine dermal cells) densely staining spherical, elliptieM and elongated particles were detected (2, 9) . Thin sections through BRV infected intestinal cells of calves (10, 11, 12) showed elongated viral particles with rounded ends measuring 42 × 100.5 nm. The high molecular weight virion protein in the range of 75-100 kD of BEV is glycosylated, probably by N-linked oligosaccharides since tunicamycin, an antibiotic known to inhibit N-linked oligosaccharide synthesis, prevented the formation of infectious virus as well as appearance of the 75-100 kD band in PAGE of infected cells (20) . Evidence of a reaction of the particles in human feces with sera containing antibodies against BRV and BEV, respectively, were obtained in IEM (8), (Flewett personal communication). ./cache/cord-004775-foaf3vyl.txt ./txt/cord-004775-foaf3vyl.txt