id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004830-vmka378d	Cursiefen, Dagmar	In vitro cultivation of cells from the chorioallantoic membrane of chick embryos	1975		.txt	text/plain	2554	177	57	By treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens. In an attempt to plaque-purify and to quantitatc some strains of influenza viruses and of avian reoviruses we succeeded to cultivate cells from the chorioallantoic membrane of chick embryos. The following strains were tested for their ability to grow, induce a cytopathie effect and form plaques in chorioallantoic cells of the chick embryo (CAM-cells). After an incubation period of 8 to 16hrs the media were assayed for hemagglutinating activity and for infectivity by plaque tests on the same cell type used for growing the viruses, i.e. CAM cells or chick embryo fibroblasts.	./cache/cord-004830-vmka378d.txt	./txt/cord-004830-vmka378d.txt