id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005246-cskb0njm	Ludwig, George V.	Insect-transmitted vertebrate viruses: Flaviviridae	1993		.txt	text/plain	8060	416	41	Additionally, these culture systems permit the study of flavivirus attachment, penetration, replication, and release from cells and have been instrumental in the production and characterization of live-attenuated vaccines. Cell culture is essential for the study of flaviviruses, and will continue to play a pivotal role in the isolation, characterization, and development of new vaccines against current and future flavivirus health threats. More recent research has shown that fusion of dengue, yellow fever, and Japanese encephalitis viruses may in fact involve fusion of virus directly to the cell's plasma membrane (52, 108) . The observation that persistent dengue virus infections can be established in a non-vector mosquito cell line associated with changes in several of the important biological characteristics, such as temperature sensitivity and antigenic structure (64) , presents the possibility that genetic selection of attenuated virus strains may be possible with insect cell lines.	./cache/cord-005246-cskb0njm.txt	./txt/cord-005246-cskb0njm.txt