id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-008454-8brxpotx	Field, Anne M.	Diagnostic Virology Using Electron Microscopic Techniques	2008-04-09		.txt	text/plain	18793	981	50	The morphology of negatively stained virus particles is sufficient for grouping purposes but it is necessary to use immune electron microscopy (IEM) to differentiate morphologically identical but antigenically distinct viruses. Virus particles are sometimes present in such large numbers in clinical specimens that they can be detected directly by electron microscopy and negative staining methods in particular can be used to provide a rapid diagnosis. Immune electron microscopy on sectioned material presents considerable technical difficulties and viral content of tissue homogenates may be too low for negative stain IEM so a virus seen in the tissue cannot always be sufficiently well identified for diagnostic purposes. Similarly, thin section studies on livers of marmosets infected with hepatitis A virus showed cytoplasmic picornavirus-like particles which could be extracted for use as antigen to detect antibodies by negative stain IEM (Provost et al., 197513) and by complement fixation (Provost et al., 1975a) .	./cache/cord-008454-8brxpotx.txt	./txt/cord-008454-8brxpotx.txt