id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-008700-knbf8m4x	Rodrigues, Merlyn R.	Methods for Rapid Detection of Human Ocular Viral Infections	2013-10-30		.txt	text/plain	3011	171	38	Simple techniques of immunofluorescence and negative stain electron microscopy are used for the rapid detection of viruses in human adenoviral, herpetic, rubella, molluscum contagiosum, and vaccinial infections. Lens aspirate from a 2-year-old patient with clinical ocular rubella was examined by immunofluorescence and negative stain electron microscopy. 10 In a recent epidemic of EKC at a Vietnamese refugee camp in Florida, adenovirus type 8 was recovered in 81% of cases cultured within two weeks of onset of infection.U Dawson et aP 2 described adenovirus-like particles in the conjunctiva of one patient and in the corneal epithelium of another by transmission electron microscopy of tissue culture preparations. In the present study, the typical virions of herpes simplex keratitis were readily identified both by immunofluorescence and by negative stain transmission electron microscopy. Herpes simplex hominis type 1 was recovered in culture and demonstrated by direct immunofluorescence and negative stain electron microscopy in three patients with herpetic dendritic keratitis.	./cache/cord-008700-knbf8m4x.txt	./txt/cord-008700-knbf8m4x.txt