id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-010188-884d196k	Schlesinger, Sondra	Alphaviruses — vectors for the expression of heterologous genes	2004-08-26		.txt	text/plain	3049	140	47	Sindbis virus and Semliki Forest vires are best known as valuable models for molecular and cell biology, and it is these two viruses that are presently being developed as vectors for the expression of heterologous genes. The basic strategy for using alphaviruses as vectors for the expression of heterologous genes has been to construct cDNAs of the alphavirus genome, in which the heterologous gene is placed downstream from the promoter used to transcribe a subgenomic RNA 13 (Fig. 2a) . A second potential problem is recombination between the packaging helper virus RNA and vector RNAs. The two Sindbis RNAs can undergo recombination to produce a single molecule of RNA containing the genes that encode both the nonstructural and structural proteins m. The initial studies with Sindbis and Semliki Forest virus suggest that both viruses are promising as vectors for heterologous gene expression.	./cache/cord-010188-884d196k.txt	./txt/cord-010188-884d196k.txt