id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-016538-4og05fuo	Dolja, V. V.	Biotechnology Applications of Grapevine Viruses	2017-03-30		.txt	text/plain	6266	292	44	Although in theory any of the grapevine-infecting viruses can be engineered into transient gene expression or VIGS vector, in practice, only one of them, the filamentous Grapevine leafroll-associated virus-2 (GLRaV-2) from the genus Closterovirus (family Closteroviridae), was demonstrated to fulfill these roles (Dolja and Koonin 2013; Kurth et al. Among these viruses, only GLRaV-2, a closterovirus, has been so far engineered into a vector capable of systemic infection of grapevine that either produces recombinant protein or elicits VIGS response (Kurth et al. The more recently developed CTV-based gene expression vectors were shown to be not only capable of systemic infection in the natural citrus hosts but also exhibited remark-able genetic stability in regard to retention of the inserted recombinant gene, as well as VIGS capability (Dawson et al. Another candidate to be developed as a vector for protein expression and VIGS is GRSPaV, which is the only grapevine-infecting member of the genus Foveavirus that was recently characterized (Meng et al.	./cache/cord-016538-4og05fuo.txt	./txt/cord-016538-4og05fuo.txt