id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-017167-8cdbcrh7	Ahola, Tero	Functions of Chikungunya Virus Nonstructural Proteins	2016-12-03		.txt	text/plain	10491	530	49	The nonstructural proteins (nsPs) of chikungunya virus (CHIKV) are expressed as one or two polyprotein precursors, which are translated directly from the viral genomic RNA. Similar to other alphaviruses, CHIKV nsPs not only perform virus RNA replication but are also crucial for other activities essential for virus infection and pathogenesis. However, recent studies of SFV P1234 processing reveal that a second mechanism, the presentation of cleavage sequences via long-range interactions between different domains of the polyprotein, Processing of CHIKV ns polyprotein P1234 and RNA synthesis. The main interaction appears to be mediated by a membrane-binding peptide located in the central part of the protein (approximately residues 244-263 in CHIKV nsP1; Fig. 2 ), which forms an amphipathic alpha helix, as characterized for the corresponding peptide from SFV (Ahola et al. However, the effects of mutations introduced into the NTPase/helicase active site were different for these viruses: in SINV such a mutation strongly reduced the nsP2-dependent degradation of Rpb1 whereas CHIKV nsP2 mostly retained its ability to block host gene expression.	./cache/cord-017167-8cdbcrh7.txt	./txt/cord-017167-8cdbcrh7.txt