id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-020969-lh2ergpm	STRAUSS, JAMES H.	Gene Therapy	2012-07-27		.txt	text/plain	11793	597	52	Together with methods for cloning and manipulating viral genomes, this information has made possible the use of viruses as vectors to express foreign genes. The use of minus-strand RNA viruses as vectors was delayed because the virion RNA itself is not infectious, but recent developments has made it possible to rescue virus from cloned DNA by using coexpression of the appropriate viral proteins in a transfected cell. It is also possible to transfect cells with the E1 or E3 expression cassette together with DNA clones encoding the rest of the adenovirus genome, in which case homologous recombination results in the production of virus. Because the poliovirus replicon lacks a full complement of the structural genes (it is a suicide vector), packaging to produce particles requires infection of a cell that expresses the polioviral structural proteins by some mechanism.	./cache/cord-020969-lh2ergpm.txt	./txt/cord-020969-lh2ergpm.txt