id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-021034-hnw7a3a1	Mahony, James B.	Negative staining in the detection of viruses in clinical specimens	2002-10-09		.txt	text/plain	4164	297	46	In our experience the serum-in-agar (SIA) method is the most sensitive of the PAG IEM techniques for detection of rotavirus particles in clinical specimens. SPEIMDAGT employing colloidal gold-labeled secondary antibody has increased sensitivity and offers the advantage of detecting viral antigen when whole virus particles are not visible. Prior to the development of immunoassay techniques for detecting viral antigens, direct electron microscopy (DEM) with negative staining was the only method of providing a definitive diagnosis of several viral infections. We have used this method to detect rotavirus in fecal specimens (Wu et a!., 1989) (Fig. 2) Solid phase JEM Although IEM is considerably more sensitive than DEM, it is dependent upon the optimal concentrations of antibody and antigen and is susceptible to a prozone phenomenon. Development of a sensitive protein A-gold immunoelectron microscopy method for detecting viral antigens in fluid specimens	./cache/cord-021034-hnw7a3a1.txt	./txt/cord-021034-hnw7a3a1.txt