id author title date pages extension mime words sentences flesch summary cache txt cord-022349-z8w1wkm8 Beeler, Judy A. Human and Animal Viruses 2007-09-02 .txt text/plain 4584 230 47 However, freeze-drying provided a way to maintain virally infected material over long periods of time with relative ease when compared to serial passage in a susceptible animal host. The first experiment to demonstrate that this method could provide long-term stability was reported for a bovine virus that had been freeze-dried in 1916 and was shown to be viable after being maintained at room temperature for 30 years (Fasquelle and Barbier, 1950) . In general, most viruses may be "snap frozen" in a dry ice/ethanol bath prior to storage at temperatures _<-70~ It may be preferable to freeze some viruses, such as cytomegalovirus or varicella-zoster virus, as viable infected cells. Calcium lactobionate and human serum albumin were cryoprotective for measles virus during freeze-drying (Greiff and Rightsel, 1967) , whereas cell culture medium alone was less protective. ./cache/cord-022349-z8w1wkm8.txt ./txt/cord-022349-z8w1wkm8.txt