id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-022354-aqtceqqo	HUNTER, ERIC	Membrane Insertion and Transport of Viral Glycoproteins: A Mutational Analysis	2012-12-02		.txt	text/plain	17747	697	44	second apolar region in gp 37 consists of a 27 amino acid long stretch of hydrophobic residues near the carboxy terminus that functions during translation to stop the movement of the protein into the lumen of the rough endoplasmic reticulum (RER) and to anchor the complex in the membrane. In order to examine the role of the signal peptide in RSV glycoprotein biosynthesis we constructed a series of deletion mutations within the 5' coding region of the env gene using the double-stranded exonuclease BaBl. Oligonucleotide linkers of the sequence CATCGATG were ligated to the ends of the truncated molecules to introduce a unique restriction endonuclease cleavage site and to replace the deleted in-frame AUG. We found that replacement of the nonconserved region of the cytoplasmic domain with a longer unrelated sequence of amino acids from SV40 vector sequences (mutant Cl) did not alter the rate of transport to the Golgi apparatus nor the appearance of the glycoprotein on the cell surface.	./cache/cord-022354-aqtceqqo.txt	./txt/cord-022354-aqtceqqo.txt