id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-023678-9q68ftr9	Hierholzer, J.C.	Virus isolation and quantitation	2007-09-02		.txt	text/plain	7674	425	61	The method conserves the virus and viral antigens, because the supernatant fluid is decanted from the cell culture into a sterile tube at the end of the incubation period (7-10 days); the monolayer is washed twice with 2-3 ml of plain Hanks Balanced Salt Solution (HBSS) at room temperature; 1 ml of fresh HBSS followed by 0.2 ml of 0.4% mammalian erythrocyte suspension from the appropriate species is added to the monolayer; the tube is incubated stationary with the fluid covering the monolayer; and the test is read 3 times at 20-min intervals by agitating the tube in a sideways motion and then observing the monolayer at 40-100x magnification to see if the erythrocytes are firmly attached to the cultured cells or are floating free in the fluid (see Fig. 15 .4).	./cache/cord-023678-9q68ftr9.txt	./txt/cord-023678-9q68ftr9.txt