id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-254963-cnvxlv6h	Paskey, Adrian C.	Enrichment post-library preparation enhances the sensitivity of high-throughput sequencing-based detection and characterization of viruses from complex samples	2019-02-26		.txt	text/plain	6201	267	45	In order to test this newly expanded probe panel and to specifically assess the effect of hybridization-based viral enrichment on the sensitivity of HTS for detection of a single virus within a complex environmental sample, commercial bat guano was spiked with increasing concentrations of Influenza virus (IFV). As expected, a dose-dependent effect in the proportion of sequencing reads derived from IFV was observed as the number of spiked genome copies increased ( Fig. 1a and Additional file 1: Table S1 ), in both the unbiased shotgun sequence data as well as the virus enriched sequence data. Such limitations have been of particular concern for U.S. Department of Defense (DoD) laboratories tasked with biosurveillance and biodefense activities in regions with limited material resources and human We demonstrate here that hybridization-based viral target enrichment yields robust coverage of small genomes from clinical samples, even yielding full-length, deeply covered genomes at concentrations whereby Fig. 3 Detection of close relative viruses irrespective of extensive multiplexing.	./cache/cord-254963-cnvxlv6h.txt	./txt/cord-254963-cnvxlv6h.txt