id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-257064-iafm3pcc	Kint, Joeri	Quantification of Infectious Bronchitis Coronavirus by Titration In Vitro and In Ovo	2014-12-18		.txt	text/plain	1809	156	63	During a titration assay, tissue cultures or embryonated eggs are incubated with tenfold serial dilutions of a virus containing sample and several days later the cytopathic effect is scored. The virus titer is defined as the reciprocal of the dilution at which 50 % of the inoculated embryos or tissue cultures show CPE. Passaging of IBV in either embryonated eggs or primary cell cultures leads to attenuation of the virus in vivo [10] [11] [12] . IBV strains which have been adapted to grow in cultures of primary chicken cells can be titrated on these cells using either the TCID 50 method or plaque titration. Virus titers in the original sample, expressed as 10 log EID 50 /ml are calculated using the method described by Spearman and Kaerber [6, 7] , using the following formula: Plaque formation by infectious bronchitis virus in chicken embryo kidney cell cultures Growth kinetics of embryo-and organ-culture adapted Beaudette strain of infectious bronchitis virus in embryonated chicken eggs	./cache/cord-257064-iafm3pcc.txt	./txt/cord-257064-iafm3pcc.txt