id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-258489-pyfc7jde	Lico, Chiara	Viral vectors for production of recombinant proteins in plants	2008-03-10		.txt	text/plain	11091	527	39	In this review, we will focus on transient production strategies using plant viral expression systems, with a particular focus on the variety of proteins produced, and their applications. The unique properties of viruses such as ease of manipulation, high level amplification, site specific recombination, strong infectivity, enhanced translation and compact and repetitive morphological structure have enabled their broad application, from basic research to product development, including the generation of robust expression systems. From the discovery of viruses in 1898 (tobacco mosaic virus, TMV) (Bos, 1999) , to the first demonstration of RNAs role in virus replication by turnip yellow mosaic virus (TYMV) (Matthews, 1989) , to the very recent discovery of gene silencing and its implication in host response to infection, gene regulation and transgene expression (Baulcombe, 1999; Lu et al., 2003; Waterhouse and Helliwell, 2003) , plant virology has played a crucial role in the understanding of the most fundamental concepts of modern biology. Thanks to the recent improvements of viral-based vectors, mAbs have been produced with transient expression systems to quickly achieve much higher production levels along with other complex proteins.	./cache/cord-258489-pyfc7jde.txt	./txt/cord-258489-pyfc7jde.txt