id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260690-h5pjv2dw	Druce, Julian	Laboratory diagnosis and surveillance of human respiratory viruses by PCR in Victoria, Australia, 2002–2003	2004-11-12		.txt	text/plain	3803	201	45	A total of 333 additional respiratory specimens, including 20 from asymptomatic laboratory staff was used to validate the PCR assays for influenza A virus (H1 and H3 subtypes), influenza B virus, RSV, parainfluenza viruses (at least one of types 1-3), picornaviruses (a mixture of enteroviruses and rhinoviruses), and adenoviruses (each of different serotype) (Table III) . The process included the design and evaluation of primers; optimization of nucleic acid extraction conditions; establishment of optimum PCR amplification conditions; evaluation of applicable specimen types; determination of assay sensitivity compared to conventional assays; specificity testing using clinical material likely to be negative (including asymptomatic staff volunteers); or material previously shown to be positive for respiratory viruses by conventional assays or by sequencing of an amplified product where no other confirmatory method was available.	./cache/cord-260690-h5pjv2dw.txt	./txt/cord-260690-h5pjv2dw.txt