id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-261171-iknoqb4d	Roingeard, Philippe	Viral detection by electron microscopy: past, present and future	2012-01-09		.txt	text/plain	4446	227	44	In research, new imaging techniques for fluorescence light microscopy have supplanted TEM, making it possible to study live cells and dynamic interactions between viruses and the cellular machinery. This large diversity of viruses potentially involved in human gastroenteritis contributed to the use of TEM on negatively stained samples for routine diagnosis by this rapid 'catch-all' method in clinical virology ( Figures 2C and 2D ). The new techniques, which can be used on living cells, make it possible to follow the fate of individual viral particles and to monitor dynamic interactions between viruses and cellular structures, making it possible to study steps in infection that could previously not be observed. HIV morphogenesis also provides a remarkable example of intensive research into virus-host cell interactions, for which the debate concerning the Figure 6 Budding of HCV Ultrastructural analysis of cells producing the HCV core protein shows that this protein self-assembles into HCV-like particles (arrows) at convoluted and electron-dense ER membranes surrounding the lipid droplets (LD) present in the perinuclear area.	./cache/cord-261171-iknoqb4d.txt	./txt/cord-261171-iknoqb4d.txt