id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263157-8jin6oru	Martínez, Miguel Angel	Progress in the Therapeutic Applications of siRNAs Against HIV-1	2008-10-13		.txt	text/plain	9234	466	45	Recent advances regarding the utility of RNA-mediated interference (RNAi) to specifically inhibit HIV-1 replication have opened new possibilities for the development of gene-based therapies against HIV-1 infection. Importantly, this study made the extraordinary demonstration that cell transfection of synthetic 21 base pairs (bp) short interfering RNA (siRNA) duplexes can mediate RNAi in a sequence-specific manner; this finding enabled the specific regulation of gene expression in a variety of biological systems, including diseased cells. Soon after the demonstration that synthetic siRNAs were able to induce the RNAi mechanism in mammalian cells (15) , several studies reported that HIV-1 gene expression and replication ex vivo could be inhibited by virus-specific synthetic siR-NAs (16 22) or expressed siRNAs (16, 18) that were targeted to early or late phases of virus replication. To counteract this strategic weakness, co-expression of multiple siRNAs or shRNAs that target conserved RNA sequences could reduce the emergence of single siRNA-resistant virus with a comparable effect to that achieved by the multiple anti-HIV drug combination approach employed by HAART.	./cache/cord-263157-8jin6oru.txt	./txt/cord-263157-8jin6oru.txt