id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267140-vdcf6vok	Trudel, M.	Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide	1981-02-28		.txt	text/plain	1718	105	45	title: Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide Yields were lower in sucrose and metrizamide, while sodium metrizoate reduced the infectivity of the virus below detectable levels. This report describes the application of Sepharose 2B gel fitration for the purification of infectious rubella virus in comparison to centrifugation on sucrose, metrizamide and sodium metrizoate. Aliquots of 5 ml of viruses were purified by two cycles of density gradient centrifugation in sucrose, metrizoic acid (sodium salt) or metrizamide (Sigma Chemical Co., St. Louis, MO). Density gradient centrifugation of rubella virus on sucrose, metrizamide or sodium a Virus (5 ml, 65,566 HAU and 109*6TCID,,) was purified by ultracentrifugation or chromatography as described in Material and methods. Purification of rubella virus yielded similar results in sucrose and metrizamide but the infectivity was reduced to undetectable levels in sodium metrizoate.	./cache/cord-267140-vdcf6vok.txt	./txt/cord-267140-vdcf6vok.txt