id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-267733-fuz8r3vj	Al Ali, Sally	Use of Reporter Genes in the Generation of Vaccinia Virus-Derived Vectors	2016-05-21		.txt	text/plain	7966	392	41	This broad host range allows infection of cell lines with recombinant viruses for large-scale expression of heterologous proteins, which reduces its cost This broad host range allows infection of cell lines with recombinant viruses for large-scale expression of heterologous proteins, which reduces its cost in comparison to other production systems [21, 24] . This reporter gene system has been widely used in transgenic plants, and it has also been successfully used in mammalian cells for VACV recombinant virus selection [54] . Reporter-gene assays have helped the pox virologists in basic research, for example for the study of the location, structure and function of many VACV proteins during the infection cycle and their interaction with proteins of the host cell [44, 70] . The main limitation of using VACV as a vector is the short-term gene expression, since it is a lytic virus killing the infected cells. Insertion sites for recombinant vaccinia virus construction: Effects on expression of a foreign protein	./cache/cord-267733-fuz8r3vj.txt	./txt/cord-267733-fuz8r3vj.txt