id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274112-6t0wpiqy	Webby, RJ	Responsiveness to a pandemic alert: use of reverse genetics for rapid development of influenza vaccines	2004-04-03		.txt	text/plain	4199	206	48	INTERPRETATION: The ability to produce a candidate reference virus in such a short period of time sets a new standard for rapid response to emerging infectious disease threats and clearly shows the usefulness of reverse genetics for influenza vaccine development. The agent must be handled only under conditions of at least biosafety level 3 (BSL3), and it can kill fertilised chicken eggs, the standard medium for the reassortment and Responsiveness to a pandemic alert: use of reverse genetics for rapid development of influenza vaccines propagation of influenza virus before its inactivation and formulation for use in vaccines. The vaccine-candidate reference virus stock described in this report has been produced entirely on a cell substrate licensed for the manufacture of human vaccine, and as such, is-to our knowledge-the first reverse genetically derived influenza vaccine suitable for testing in clinical trials. Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses	./cache/cord-274112-6t0wpiqy.txt	./txt/cord-274112-6t0wpiqy.txt