id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-274765-3wzht843	Kweon, Chang-Hee	Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate	1999-06-04		.txt	text/plain	2518	133	55	The field isolate of porcine epidemic diarrhea virus (PEDV) was serially passaged in Vero cells. The cell passaged PEDV, designated KPEDV-9, was tested for its pathogenicity in the neonatal pigs, immunogenicity and safety in the pregnant sows. The results of this study supported that the attenuated virus derived from serial passage could be applied as vaccine for protecting suckling piglets against PEDV infection. In this study, we investigated the attenuation of PEDV through serial passages in Vero cell cultures and its prophylactic eect in pregnant sows. Nevertheless, when compared with the wild PEDV, the animals inoculated with the high passage level of virus did not show any severe signs of diarrhea or death in piglets, supporting attenuation. Development of an Elispot for the detection of antibody secreting cells against the porcine epidemic diarrhea virus (PEDV) in dierent tissues	./cache/cord-274765-3wzht843.txt	./txt/cord-274765-3wzht843.txt