id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-276052-gk6n8slx	Yadav, Pragya	Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India	2016-09-09		.txt	text/plain	3005	164	51	During the survey for Nipah virus among bats at North-East region of India; Tioman virus (TioV), a new member of the Paramyxoviridae family was isolated from tissues of Pteropus giganteus bats for the first time in India. While investigating NiV in urine samples of giant fruit bats of the Pteropus genus on Tioman Island, Malaysia, in 2001, researchers isolated a novel virus which was placed in the Rubulavirus genus of the Paramyxoviridae family. In order to study susceptibility of different vertebrate cells to TioV, the infectious virus titer was determined by estimating 50% tissue culture infective dose (TCID 50 ) using Reed and Muench method (Reed and Muench, 1938) . Negative contrast electron microscopy of the cell supernatant of Vero CCL-81 infected with virus isolate showed the presence of virus particles with the typical paramyxovirus morphology. TioV isolated from kidney tissue homogenate of bat showed a titer of 10 4.61 /100 μL by TCID 50 in Vero CCL-81 cell line.	./cache/cord-276052-gk6n8slx.txt	./txt/cord-276052-gk6n8slx.txt