id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-280781-u3wd27rn	Stohlman, S. A.	Stability of neurotropic mouse hepatitis virus (JHM strain) during chronic infection of neuroblastoma cells	1978		.txt	text/plain	3509	184	50	title: Stability of neurotropic mouse hepatitis virus (JHM strain) during chronic infection of neuroblastoma cells A line of mouse neuroblastoma cells which was chronically infected with the neurotropic strain (JHM) of MHV, a member of the coronavirus group, was established. The addition of low concentration antiviral antibody modulated the infection to a carrier culture with viral antigen in the cytoplasm of the cells, but no infectious virus was produced, and the cells lacked both surface viral antigen and CPE. Following incubation at room temperature for 30 minutes in the presence of anti-JHM hyperimmune aseitie fluid (50 per cent plaque reduction neutralization titer = 1/1~00), serial dilutions were plated on the indicator monolayers and fixed with 0.5 ml of DMEM plus 5 per cent FBS containing 0.6 per cent agarose. Figure 3 shows that following the initial passage in the presence of antibody, there was an increase in both the supernatant and cell associated virus titer, which rapidly declined until after 4 serial passages no infectious virus was detectable.	./cache/cord-280781-u3wd27rn.txt	./txt/cord-280781-u3wd27rn.txt