id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281429-6lv3di4x	García-Nicolás, Obdulio	Targeting of the Nasal Mucosa by Japanese Encephalitis Virus for Non-Vector-Borne Transmission	2018-11-27		.txt	text/plain	7414	396	49	Using nasal mucosal tissue explants and primary porcine nasal epithelial cells (NEC) at the air-liquid interface (ALI) and macrophages as ex vivo and in vitro models, we determined that the nasal epithelium could represent the route of entry and exit for JEV in pigs. Porcine NEC at the ALI exposed to with JEV resulted in apical and basolateral virus shedding and release of monocyte recruiting chemokines, indicating infection and replication in macrophages. Using nasal mucosa tissue explants and three-dimensional porcine nasal epithelial cells cultures and macrophages as ex vivo and in vitro models, we determined that the nasal epithelium could be a route of entry as well as exit for the virus. In fact, our data demonstrate that JEV has the ability of infecting apically, resulting in both apical and basolateral virus shedding in swine nasal epithelial cells and indicating that the porcine nasal mucosa could represent a gateway for JEV entry and exit in pigs.	./cache/cord-281429-6lv3di4x.txt	./txt/cord-281429-6lv3di4x.txt