id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-282343-cko4curf	Cheng, Han	A parallel genome-wide RNAi screening strategy to identify host proteins important for entry of Marburg virus and H5N1 influenza virus	2015-11-24		.txt	text/plain	5071	278	56	RESULTS: The parallel nature of the strategy allows us to easily identify the host factors for a specific virus with a greatly reduced number of false positives in the initial screen, which is one of the major problems with high throughput screening. This strategy was used for an RNAi screen to identify host proteins specific for the entry process of Marburg virus (MARV) or avian influenza virus H5N1 (AIV), demonstrating the utility of this approach. The key feature of the protocol is that AIV and siRNA showing low signals in assay plates of both viruses is regarded as a "shared" hit by the two viruses MARV pseudovirions were used in parallel in the RNAi screen which allowed us to reduce the number of false positives and to quickly identify Marburg-specific and flu-specific host factors (which will be further discussed below).	./cache/cord-282343-cko4curf.txt	./txt/cord-282343-cko4curf.txt