id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-292643-n6xp5mlz	Hall, Richard J.	Evaluation of rapid and simple techniques for the enrichment of viruses prior to metagenomic virus discovery	2013-09-13		.txt	text/plain	4803	219	44	The relative abundance of a virus (or viral nucleic acid) in a sample, compared to that of other organisms such as bacteria or host cells (or their genomes), is a critical factor for the discovery of viruses when using metagenomics. A study on human liver tissue compared enrichment techniques of freeze-thaw, centrifugation and nuclease-treatment for the detection of Hepatitis C Virus using both Roche 454 and Illumina high-throughput sequencing platforms (Daly et al., 2011) . After an initial 10 min reverse transcription step at 45 • C and 10 min denaturation Table 1 Virus enrichment process prior to sequencing in metagenomic studies on human and animal samples. This artificial sample represents a starting point to evaluate simple and rapid viral enrichment methods for use in virus metagenomics studies that seek to detect a virus that is causing disease in humans or animals.	./cache/cord-292643-n6xp5mlz.txt	./txt/cord-292643-n6xp5mlz.txt