id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298051-ej8qxkce	Louten, Jennifer	Detection and Diagnosis of Viral Infections	2016-05-06		.txt	text/plain	11204	602	57	Cell lines can be infected with patient samples to allow viral replication within the cells; observable cytopathic effects can help to identify the identity of the virus. Infected cells can also be used for immunofluorescence assays, which use fluorescently labeled virus-specific antibodies to identify viruses in fixed cells or tissues. In the process of PCR, DNA (including any viral DNA present) is isolated from the clinical specimen, generally blood cells or tissue, and added to a tube containing primers, DNA polymerase, and nucleotides ( Fig. 7.14) . The diagnostic techniques described in this chapter identify the presence of a virus in a sample, or even the amount of viral nucleic acid, but these assays cannot determine the amount of virus present that is capable of productively infecting cells. Fluorescently labeled antibodies bind to viral antigens present in infected cells.	./cache/cord-298051-ej8qxkce.txt	./txt/cord-298051-ej8qxkce.txt