id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300837-d0a8y5qh	Khetawat, Dimple	A Functional Henipavirus Envelope Glycoprotein Pseudotyped Lentivirus Assay System	2010-11-12		.txt	text/plain	8988	355	44	To circumvent this problem, we have developed a henipavirus envelope glycoprotein pseudotyped lentivirus assay system using either a luciferase gene or green fluorescent protein (GFP) gene encoding human immunodeficiency virus type-1 (HIV-1) genome in conjunction with the HeV and NiV fusion (F) and attachment (G) glycoproteins. Pseudotyped virus particles generated with the NiV F and G glycoproteins were able to infect and produce luciferase reporter gene activity at various levels on all permissive receptor expressing cells ( Figure 1A ) while no signal was observed with the receptor negative HeLa-USU or with control virus particles generated by transfection with empty vector (pCAGGs). To confirm these findings and demonstrate an expanded utility of the henipavirus envelope glycoprotein pseudotyping systems, NiV and HeV F and G glycoprotein bearing lentivirus particles were prepared with the GFP reporter gene encoding construct pNL4-3-GFP-E-R + and used to infect receptor positive 293T cells ( Figure 2 ).	./cache/cord-300837-d0a8y5qh.txt	./txt/cord-300837-d0a8y5qh.txt