id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302047-vv5gpldi	Willemsen, Anouk	On the stability of sequences inserted into viral genomes	2019-11-14		.txt	text/plain	12557	598	43	Viruses are widely used as vectors for heterologous gene expression in cultured cells or natural hosts, and therefore a large number of viruses with exogenous sequences inserted into their genomes have been engineered. Viruses genera covered in relevant studies Conclusions of this review All viruses • Inserted sequences are often unstable and rapidly lost upon passaging of an engineered virus • The position at which a sequence is integrated in the genome can be important for stability • Sequence stability is not an intrinsic property of genomes because demographic parameters, such as population size and bottleneck size, can have important effects on sequence stability • The multiplicity of cellular infection affects sequence stability, and can in some cases directly affect whether there is selection for deletion variants • Deletions are not the only class of mutations that can reduce the cost of inserted sequences, although they are the most common I: dsDNA	./cache/cord-302047-vv5gpldi.txt	./txt/cord-302047-vv5gpldi.txt